Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,464 G→A 6.3% S1009S (TCG→TCA sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,4640GA 6.3% 942.3 / 10.4 320S1009S (TCG→TCAsdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base G (126/164);  new base A (8/21);  total (134/186)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 324 total aligned reads displayed.

AGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCA  >  USA300TCH1516_ALE/622374‑622554
                                                                                          |                                                                                          
   ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                                         <  1:4775348/91‑1 (MQ=39)
     cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                       >  1:5340461/1‑91 (MQ=37)
     cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                       >  1:6886844/1‑91 (MQ=37)
      ggACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCa                                                                                      >  1:130790/1‑91 (MQ=35)
      ggACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCa                                                                                      <  2:6320047/91‑1 (MQ=34)
       gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                                                     >  1:362263/1‑91 (MQ=34)
        aCAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                                    <  1:4374046/91‑1 (MQ=33)
         cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACATCGACTCAGATTCAGATAGCGATTCGGACTCAGAc                                                                                   <  2:4145543/91‑1 (MQ=18)
         cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAc                                                                                   <  2:5471659/91‑1 (MQ=33)
          aGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACa                                                                                  >  2:1225487/1‑91 (MQ=34)
           gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAg                                                                                 >  2:4335850/1‑91 (MQ=255)
            cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGc                                                                                <  1:2416352/91‑1 (MQ=34)
            cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACCCAGACAGc                                                                                >  1:3594823/1‑91 (MQ=18)
             gATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCg                                                                               >  1:5740356/1‑91 (MQ=33)
              aTTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGa                                                                              <  2:492955/91‑1 (MQ=32)
                     cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc                                                                       <  2:1801836/91‑1 (MQ=31)
                     cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc                                                                       >  1:2982958/1‑91 (MQ=31)
                     cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc                                                                       >  1:2452870/1‑91 (MQ=31)
                     cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGAc                                                                       >  2:6178096/1‑91 (MQ=14)
                       cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTc                                                                     >  1:401183/1‑91 (MQ=33)
                        aGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCa                                                                    >  2:303762/1‑91 (MQ=33)
                        aGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCa                                                                    <  2:6451762/91‑1 (MQ=34)
                        aGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCa                                                                    <  1:4706244/91‑1 (MQ=34)
                         gATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAg                                                                   <  1:558750/91‑1 (MQ=34)
                         gATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGAGTCAGACTCAg                                                                   >  2:3533374/1‑91 (MQ=255)
                            aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATa                                                                >  1:978049/1‑91 (MQ=37)
                             gCGTCTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                               <  1:3291348/91‑1 (MQ=37)
                             gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                               <  1:5913894/91‑1 (MQ=38)
                             gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                               <  1:275214/91‑1 (MQ=38)
                             gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                               <  1:2817540/91‑1 (MQ=38)
                             gCGACTCAGAGTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGCCTCAGACAGCGATTCAGACTCAGATAg                                                               <  2:5793975/91‑1 (MQ=255)
                               gACTAAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCg                                                             <  2:1832623/91‑1 (MQ=255)
                                aCTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCga                                                            <  1:2062357/91‑1 (MQ=39)
                                 cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGAGTCAGAGAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgag                                                           >  2:844590/1‑90 (MQ=255)
                                 cTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                           <  2:4803058/91‑1 (MQ=255)
                                  tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgact                                                          <  2:4067693/91‑1 (MQ=39)
                                   cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactc                                                         >  1:253067/1‑91 (MQ=39)
                                      attcagatAGGGATGCAGACTCAGACAGCGACTGAGATTCAGATAGCGATTCGGACTCAAATAGCGATTCAGACGCAGATAGCgactcaga                                                      <  1:6405119/91‑1 (MQ=14)
                                      attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcaga                                                      <  1:885686/91‑1 (MQ=39)
                                      attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcaga                                                      <  1:3268683/91‑1 (MQ=39)
                                      attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcaga                                                      <  1:6355341/91‑1 (MQ=39)
                                      attcagatAGCGATTCAGACTCAGACAGCGAATCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcaga                                                      <  1:6502690/91‑1 (MQ=37)
                                       ttcagatGGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                     <  1:5551745/91‑1 (MQ=255)
                                       ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                     <  2:1378020/91‑1 (MQ=39)
                                       ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                     <  2:393664/91‑1 (MQ=39)
                                        tcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagact                                                    >  2:1474433/1‑91 (MQ=37)
                                         cagagAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                   <  2:5372572/91‑1 (MQ=255)
                                           gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                 <  1:6980060/91‑1 (MQ=255)
                                           gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                 <  2:740144/91‑1 (MQ=255)
                                           gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                 >  2:1030445/1‑91 (MQ=39)
                                            atAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga                                                <  1:1808474/91‑1 (MQ=255)
                                            atAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga                                                <  2:2305267/91‑1 (MQ=255)
                                             tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga                                                <  1:6685855/90‑1 (MQ=255)
                                             tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga                                                >  2:6685855/1‑90 (MQ=255)
                                               gCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                             >  1:392828/1‑91 (MQ=255)
                                               gCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                             <  1:4529883/91‑1 (MQ=255)
                                                cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGc                                            >  1:1594207/1‑91 (MQ=255)
                                                cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGc                                            >  1:3842016/1‑91 (MQ=255)
                                                cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGc                                            <  2:1387999/91‑1 (MQ=255)
                                                  aTTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGa                                          <  1:3433327/91‑1 (MQ=255)
                                                  aTTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGa                                          <  2:2599214/91‑1 (MQ=255)
                                                   ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                         <  1:2276386/91‑1 (MQ=255)
                                                   ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                         >  2:1998154/1‑91 (MQ=255)
                                                    tcagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACt                                        <  1:104261/91‑1 (MQ=255)
                                                    tcagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACt                                        <  1:4868791/91‑1 (MQ=255)
                                                      agactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtca                                      <  2:6787520/91‑1 (MQ=255)
                                                       gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcag                                     >  2:6223591/1‑91 (MQ=255)
                                                         ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagac                                   >  2:1433584/1‑90 (MQ=255)
                                                            agacAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattca                                <  2:2516325/91‑1 (MQ=255)
                                                             gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcag                               <  1:349950/91‑1 (MQ=255)
                                                              acAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcaga                              <  1:3113013/91‑1 (MQ=255)
                                                              acAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcaga                              <  1:5231302/91‑1 (MQ=255)
                                                              acAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGActcagactcaga                              >  2:1760340/1‑91 (MQ=255)
                                                               cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcagat                             <  1:1225487/91‑1 (MQ=255)
                                                               cAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcagat                             <  2:1111771/91‑1 (MQ=255)
                                                               cAGCGACTCAGATTCAGACAGCGACTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcagat                             <  2:6063560/91‑1 (MQ=255)
                                                                 gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAg                           >  2:5550153/1‑91 (MQ=255)
                                                                  cGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                          <  2:3255735/91‑1 (MQ=255)
                                                                    aCTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGa                        <  1:1153845/91‑1 (MQ=255)
                                                                     cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGaa                       >  2:3788410/1‑90 (MQ=255)
                                                                      tcagattcagatCGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAtt                      <  2:4496680/91‑1 (MQ=255)
                                                                        agattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCg                    <  1:611678/91‑1 (MQ=255)
                                                                         gattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCgg                   >  1:140386/1‑91 (MQ=255)
                                                                         gattcagacAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAAATAGCGATTCgg                   <  2:6511096/91‑1 (MQ=255)
                                                                          attcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                  <  1:4946206/91‑1 (MQ=255)
                                                                          attcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                  >  1:4999031/1‑91 (MQ=255)
                                                                          attcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                  <  1:6305836/91‑1 (MQ=255)
                                                                             cagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTc               >  2:883163/1‑91 (MQ=255)
                                                                                atAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa            <  1:202098/91‑1 (MQ=255)
                                                                                atAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa            <  2:5185039/91‑1 (MQ=255)
                                                                                   gCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg         <  2:2671955/91‑1 (MQ=255)
                                                                                      aTTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa      <  2:1618615/91‑1 (MQ=255)
                                                                                       ttCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc     <  2:426104/91‑1 (MQ=255)
                                                                                       ttCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAAAGCGAc     >  2:6408084/1‑91 (MQ=255)
                                                                                        tCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACt    <  1:4211004/91‑1 (MQ=255)
                                                                                         cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTc   >  1:790393/1‑91 (MQ=255)
                                                                                         cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTc   >  2:2922581/1‑91 (MQ=255)
                                                                                         cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTc   >  2:3435766/1‑91 (MQ=255)
                                                                                         cGGACTCAGACAGCGATTCAGACGCAGATAGCGACTCAGACGCAGACAGCGACTCAGATTCAGATAGCGATGCGGACTCAGATAGCGACTc   <  2:3740369/91‑1 (MQ=17)
                                                                                          ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCa  >  2:298824/1‑91 (MQ=255)
                                                                                          |                                                                                          
AGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCA  >  USA300TCH1516_ALE/622374‑622554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: