Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 99,276 G→C 6.9% intergenic (+70/+2) USA300HOU_RS00465 → / ← tetA_1 hypothetical protein/Tetracycline resistance protein, class C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE99,2760GC 6.9% 1559.3 / 87.2 433intergenic (+70/+2)USA300HOU_RS00465/tetA_1hypothetical protein/Tetracycline resistance protein, class C
Reads supporting (aligned to +/- strand):  ref base G (196/206);  new base C (14/16);  total (210/223)
Fisher's exact test for biased strand distribution p-value = 8.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

Only 100 of 450 total aligned reads displayed.

AAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAA  >  USA300TCH1516_ALE/99186‑99366
                                                                                          |                                                                                          
  aaaTGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGt                                                                                          >  1:2012801/1‑91 (MQ=255)
  aaaTGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGt                                                                                          <  2:3051848/91‑1 (MQ=255)
   aaTGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACgggc                                                                                         <  2:5652817/91‑3 (MQ=255)
   aaTGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTc                                                                                         <  2:5364706/91‑1 (MQ=255)
   aaTGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTc                                                                                         <  1:2135243/91‑1 (MQ=255)
    aTGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCa                                                                                        <  1:1413708/91‑1 (MQ=255)
    aTGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTCa                                                                                        <  1:6118256/91‑1 (MQ=255)
     tGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCAc                                                                                       >  2:5801218/1‑91 (MQ=255)
     tGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTCAc                                                                                       <  1:3863745/91‑1 (MQ=255)
      gATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCAct                                                                                      <  2:1720448/91‑1 (MQ=255)
      gATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCAct                                                                                      <  2:6633827/91‑1 (MQ=255)
      gATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTCAct                                                                                      <  2:3646871/91‑1 (MQ=255)
       acttGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCActc                                                                                     >  2:2386947/3‑91 (MQ=255)
       aTTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCActc                                                                                     <  2:4296651/91‑1 (MQ=255)
       aTTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCActc                                                                                     <  1:4288868/91‑1 (MQ=255)
       aTTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTCActc                                                                                     <  2:394158/91‑1 (MQ=255)
        tttGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCActct                                                                                    <  1:1491953/91‑1 (MQ=255)
           gAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACtcttct                                                                                 <  1:5090984/91‑1 (MQ=255)
              aaaGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTa                                                                              >  2:1528329/1‑91 (MQ=255)
              aaaGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTa                                                                              <  2:443999/91‑1 (MQ=255)
               aaGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTAt                                                                             <  2:1896717/91‑1 (MQ=255)
                   gaagtagtgatgtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGc                                                                         <  2:5549092/91‑1 (MQ=255)
                   gaagtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGc                                                                         <  2:4550066/91‑1 (MQ=255)
                    aagtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCa                                                                        >  2:4913396/1‑91 (MQ=255)
                    aagtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCa                                                                        <  2:1201955/91‑1 (MQ=255)
                     agtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCat                                                                       >  1:3788499/1‑91 (MQ=255)
                     agtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCat                                                                       >  1:480820/1‑91 (MQ=255)
                     agtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCat                                                                       >  2:5445301/1‑91 (MQ=255)
                     agtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCat                                                                       >  1:3699595/1‑91 (MQ=255)
                     agtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCat                                                                       >  1:2917804/1‑91 (MQ=255)
                      gtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCata                                                                      <  1:6028813/91‑1 (MQ=255)
                      gtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCata                                                                      >  1:5542374/1‑91 (MQ=255)
                       tagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCatat                                                                     <  2:4803961/91‑1 (MQ=255)
                        agtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCAtatt                                                                    <  2:6503629/91‑1 (MQ=255)
                          tgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCAtatttt                                                                  >  1:5299010/1‑91 (MQ=255)
                          tgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCAtatttt                                                                  <  1:1302258/91‑1 (MQ=255)
                            aagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCAtattttat                                                                >  2:2655419/1‑91 (MQ=255)
                                  gggTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGcc                                                          <  1:5182346/91‑1 (MQ=255)
                                  gggTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGcc                                                          >  1:3589583/1‑91 (MQ=255)
                                  gggTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGcc                                                          <  2:1648039/91‑1 (MQ=255)
                                  gggTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGcc                                                          >  1:1974871/1‑91 (MQ=255)
                                  gggTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGcc                                                          <  1:1640596/91‑1 (MQ=255)
                                   ggTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCt                                                         >  1:7006162/1‑91 (MQ=255)
                                   ggTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCt                                                         >  1:658280/1‑91 (MQ=255)
                                    gTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTg                                                        >  1:246413/1‑91 (MQ=255)
                                     tGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACtctt                                                                                   >  1:4110425/1‑63 (MQ=255)
                                     tGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACtctt                                                                                   <  2:4110425/63‑1 (MQ=255)
                                     tGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGt                                                       <  2:3476992/91‑1 (MQ=255)
                                      gCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGtt                                                      <  1:3440259/91‑1 (MQ=255)
                                        agagagAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGtttt                                                    <  1:3279625/91‑1 (MQ=255)
                                        agagagAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGtttt                                                    <  1:2255954/91‑1 (MQ=255)
                                        agagagAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGtttt                                                    >  2:760218/1‑91 (MQ=255)
                                            agaACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTCCATCTTTTATTTTGACTGTATTGTTa                                                >  2:2972819/1‑91 (MQ=255)
                                             gaACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTaa                                               >  2:5998979/1‑91 (MQ=255)
                                              aaCTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAAc                                              <  2:2215927/91‑1 (MQ=255)
                                               aCTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACa                                             <  1:3508045/91‑1 (MQ=255)
                                                   aGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAagca                                         <  1:673676/91‑1 (MQ=255)
                                                   aGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAagca                                         >  1:2987257/1‑91 (MQ=255)
                                                   aGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAagca                                         >  1:3202223/1‑91 (MQ=255)
                                                     cccATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAagcagc                                       >  1:745072/1‑91 (MQ=255)
                                                      ccATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAgcagca                                      <  1:5383574/91‑1 (MQ=255)
                                                       cATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGcagcag                                     <  1:4364908/91‑1 (MQ=255)
                                                       cATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGcagcag                                     >  2:704164/1‑91 (MQ=255)
                                                         tCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAgtg                                   >  1:5471282/1‑91 (MQ=255)
                                                         tCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAgtg                                   <  2:2868828/91‑1 (MQ=255)
                                                            aTAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTaa                                <  1:4660845/91‑1 (MQ=255)
                                                            aTAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTaa                                <  2:2470844/91‑1 (MQ=255)
                                                             tAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAAc                               >  1:1014345/1‑91 (MQ=255)
                                                                aTGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACaaa                            <  2:3340753/91‑1 (MQ=255)
                                                                aTGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACaaa                            <  2:5299010/91‑1 (MQ=255)
                                                                aTGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACaaa                            <  1:159253/91‑1 (MQ=255)
                                                                    tCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATg                        >  1:1179556/1‑91 (MQ=255)
                                                                    tCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTAGTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATg                        <  1:5823796/91‑1 (MQ=255)
                                                                     cGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGa                       <  1:280283/91‑1 (MQ=255)
                                                                     cGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGa                       >  2:6333628/1‑91 (MQ=255)
                                                                        ttGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTa                    <  1:5910058/91‑1 (MQ=255)
                                                                         tGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTaa                   >  2:1854904/1‑91 (MQ=255)
                                                                          gTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAg                  >  1:2600611/1‑91 (MQ=255)
                                                                           ttAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAgg                 <  1:407681/91‑1 (MQ=255)
                                                                           atAAAGAAGAGTGACCGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAgg                 >  1:5969143/2‑91 (MQ=255)
                                                                            tAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGa                <  1:663203/91‑1 (MQ=255)
                                                                            tAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGa                <  1:2388363/91‑1 (MQ=255)
                                                                                 aagaGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATaaaa           <  1:6235008/91‑1 (MQ=255)
                                                                                  agagTGACCGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAAt          >  1:6442186/1‑91 (MQ=255)
                                                                                   gagTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATg         >  1:3087048/1‑91 (MQ=255)
                                                                                   gagTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATg         >  1:4214954/1‑91 (MQ=255)
                                                                                    agTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGa        >  1:2737263/1‑91 (MQ=255)
                                                                                    agTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGa        <  1:5330466/91‑1 (MQ=255)
                                                                                    agTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGa        <  1:4538205/91‑1 (MQ=255)
                                                                                    agTGACCGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGa        >  2:1025253/1‑91 (MQ=255)
                                                                                     gTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAg       <  1:4432210/91‑1 (MQ=255)
                                                                                     gTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAg       >  2:5105387/1‑91 (MQ=255)
                                                                                     gTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAg       >  2:6415473/1‑91 (MQ=255)
                                                                                       gACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGta     <  1:6517175/91‑1 (MQ=255)
                                                                                        aCGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGtat    >  2:4357539/1‑91 (MQ=255)
                                                                                        aCGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGtat    >  1:1745773/1‑91 (MQ=255)
                                                                                        aCGGCCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTATAACAAATATGAGTAAGGATAAAATGAGtat    <  1:6848340/91‑1 (MQ=255)
                                                                                          ggTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTataa  >  2:6384451/1‑91 (MQ=255)
                                                                                          ggTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTataa  <  2:6717949/91‑1 (MQ=255)
                                                                                          ggTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTataa  >  1:879754/1‑91 (MQ=255)
                                                                                          |                                                                                          
AAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAA  >  USA300TCH1516_ALE/99186‑99366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: