Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,191,016 C→G 6.5% T241T (ACC→ACG mraY → Phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,191,0160CG 6.5% 343.0 / 11.4 104T241T (ACC→ACGmraYPhospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
Reads supporting (aligned to +/- strand):  ref base C (58/39);  new base G (2/5);  total (60/44)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 105 total aligned reads displayed.

TTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTAAAATGAGTCCGATTCATCA  >  USA300TCH1516_ALE/1190879‑1191156
                                                                                                                                         |                                                                                                                                            
ttAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCAtt                                                                                                                                           <  1:805007/141‑1 (MQ=255)
ttAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCAtt                                                                                                                                           >  1:80498/1‑141 (MQ=255)
  aGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTc                                                                                                                                         >  2:128669/1‑141 (MQ=255)
   ggAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACTATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCa                                                                                                                                        >  2:485936/1‑141 (MQ=255)
     agaAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACGATTTCAAt                                                                                                                                      >  4:273986/1‑141 (MQ=255)
     agaAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAAt                                                                                                                                      <  2:128226/141‑1 (MQ=255)
     agaAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAAt                                                                                                                                      >  2:504066/1‑141 (MQ=255)
       agaCGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACGATTTCAATCa                                                                                                                                    <  3:280026/139‑1 (MQ=255)
       aaaCGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCa                                                                                                                                    <  4:136946/141‑1 (MQ=255)
        aaCGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCAt                                                                                                                                   <  2:919578/141‑1 (MQ=255)
         aCGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATg                                                                                                                                  >  3:305825/1‑141 (MQ=255)
         aCGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATg                                                                                                                                  >  2:928906/1‑141 (MQ=255)
            gCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCtt                                                                                                                               <  2:459138/141‑1 (MQ=255)
             cAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTa                                                                                                                              >  1:638673/1‑141 (MQ=255)
             cAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTa                                                                                                                              >  3:456545/1‑141 (MQ=255)
              aaTTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTaa                                                                                                                             <  2:205400/141‑1 (MQ=255)
                ttGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATc                                                                                                                           >  2:1017011/1‑141 (MQ=255)
                  ggTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAg                                                                                                                         >  2:622299/1‑141 (MQ=255)
                   gTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAgg                                                                                                                        >  2:399749/1‑141 (MQ=255)
                   gTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAgg                                                                                                                        >  1:659127/1‑141 (MQ=255)
                       tttCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAAtt                                                                                                                    <  1:926964/141‑1 (MQ=255)
                         tCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTAt                                                                                                                  <  2:291013/141‑1 (MQ=255)
                          cTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACGATTTCAATCATGCTTAATCAGGAATTATc                                                                                                                 <  2:1070870/141‑1 (MQ=255)
                          cTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATc                                                                                                                 >  2:573665/1‑141 (MQ=255)
                           tGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCa                                                                                                                <  3:193841/141‑1 (MQ=255)
                           tGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCa                                                                                                                <  1:131178/141‑1 (MQ=255)
                            gTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCAt                                                                                                               <  2:11969/141‑1 (MQ=255)
                               tCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTaa                                                                                                            >  4:387610/1‑141 (MQ=255)
                               tCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTaa                                                                                                            <  1:1076806/141‑1 (MQ=255)
                                    aTGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAAttttt                                                                                                       <  1:1063670/141‑1 (MQ=255)
                                      gttgttTGCACTTTTAGGATTTTTACCATATAACAGTAACCCAGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTtat                                                                                                     >  1:933956/1‑141 (MQ=255)
                                       ttgttTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTtata                                                                                                    >  1:774184/1‑141 (MQ=255)
                                         gttTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCGTTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAgg                                                                                                  >  4:425497/1‑141 (MQ=255)
                                         gttTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAgg                                                                                                  >  3:95932/1‑141 (MQ=255)
                                          tttGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGt                                                                                                 <  1:1026564/141‑1 (MQ=255)
                                           ttGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGtt                                                                                                >  3:110202/1‑141 (MQ=255)
                                              caattttAGGATAATTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTATTATAGGTTTAg                                                                                             >  2:1001784/4‑141 (MQ=255)
                                                     aGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATTCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGt                                                                                      <  1:497728/141‑1 (MQ=255)
                                                      ggATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTACTCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTa                                                                                     >  4:338973/1‑141 (MQ=255)
                                                      ggATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTa                                                                                     >  4:298149/1‑141 (MQ=255)
                                                       gATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTaa                                                                                    >  1:137965/1‑141 (MQ=255)
                                                        aTTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCAtt                                                                                                                                           <  1:876785/85‑1 (MQ=255)
                                                        aTTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCAtt                                                                                                                                           >  2:876785/1‑85 (MQ=255)
                                                        aTTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAAt                                                                                   >  3:196403/1‑141 (MQ=255)
                                                          ttttACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTg                                                                                 <  1:749249/141‑1 (MQ=255)
                                                          ttttACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTg                                                                                 >  1:159049/1‑141 (MQ=255)
                                                          ttttACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTg                                                                                 <  1:847488/141‑1 (MQ=255)
                                                           tttACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACGATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGa                                                                                <  2:330037/141‑1 (MQ=255)
                                                            ttACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGaa                                                                               <  1:726628/141‑1 (MQ=255)
                                                                cATATAANATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACAt                                                                           <  2:816205/141‑1 (MQ=255)
                                                                  tataACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTa                                                                         <  2:991082/141‑1 (MQ=255)
                                                                    taACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATc                                                                       >  2:351866/1‑141 (MQ=255)
                                                                       cATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCAGGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCtgt                                                                    >  1:214590/1‑141 (MQ=255)
                                                                         ttAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCtgtta                                                                  >  3:107981/1‑141 (MQ=255)
                                                                          tAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCtgttat                                                                 >  3:261204/1‑141 (MQ=255)
                                                                          tAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCtgttat                                                                 >  3:34518/1‑141 (MQ=255)
                                                                            aCCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCtgttatgt                                                               <  2:666878/141‑1 (MQ=255)
                                                                              ccTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCtgttatgtta                                                             >  3:403585/1‑141 (MQ=255)
                                                                               cTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTAc                                                            >  2:214582/1‑141 (MQ=255)
                                                                                 gCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACGATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACaa                                                          <  1:46397/141‑1 (MQ=255)
                                                                                 gCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACaa                                                          <  4:306082/141‑1 (MQ=255)
                                                                                 gCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACaa                                                          <  2:250939/141‑1 (MQ=255)
                                                                                 gCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACaa                                                          >  1:1084659/1‑141 (MQ=255)
                                                                                  cTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAg                                                         >  3:393166/1‑141 (MQ=255)
                                                                                  cTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAg                                                         >  2:72873/1‑141 (MQ=255)
                                                                                      aGTGTTTATTGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGc                                                     <  2:961057/141‑1 (MQ=255)
                                                                                      aGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATNGAAACATTATCTNTTATGTTACAAGTCGc                                                     <  1:1038451/141‑1 (MQ=255)
                                                                                       gtgtTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCt                                                    >  2:115150/1‑141 (MQ=255)
                                                                                       gtgtTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCt                                                    >  2:362044/1‑141 (MQ=255)
                                                                                         ttatATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACGATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAg                                                  <  3:122638/138‑1 (MQ=255)
                                                                                         gtTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAg                                                  >  4:277135/1‑141 (MQ=255)
                                                                                           ttATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCt                                                <  1:5896/141‑1 (MQ=255)
                                                                                                ggAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTaaa                                           >  1:257776/1‑141 (MQ=255)
                                                                                                  agaTACAGGTAGCTTAGCTTTAGGTGGTATATTAGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAAtt                                         >  1:561130/1‑141 (MQ=255)
                                                                                                      aCAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGATACAAGTCGCTAGCTTTAAATTGACt                                     >  2:858180/1‑141 (MQ=255)
                                                                                                       cAGGTGGCTAAGCATTAGGGGGTCTGTAGGCTACGATATAAATGGTACTTACTCCGGCCTTACCCTTCTGTTTTCTATTTTTCGTATTCGTACTTGAAACGTTATATGTTATGTTACAAGTCGCTAGCTTTGAAGTGACTg                                    >  4:443200/1‑141 (MQ=255)
                                                                                                        aGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTgg                                   >  1:904559/1‑141 (MQ=255)
                                                                                                        aGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTgg                                   <  4:290022/141‑1 (MQ=255)
                                                                                                        aGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTgg                                   <  4:289733/141‑1 (MQ=255)
                                                                                                         ggTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGa                                  >  2:978341/1‑141 (MQ=255)
                                                                                                                tagcttTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGta                           <  1:717560/141‑1 (MQ=255)
                                                                                                                 agcttTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTTTGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGtat                          >  1:513562/1‑141 (MQ=255)
                                                                                                                 agcttTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGtat                          <  1:313238/141‑1 (MQ=255)
                                                                                                                 agcttTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGtat                          >  1:513568/1‑141 (MQ=255)
                                                                                                                   cttTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTatat                        >  3:439632/1‑141 (MQ=255)
                                                                                                                   cttTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTatat                        <  1:303970/141‑1 (MQ=255)
                                                                                                                     ttAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATAttt                      <  1:399749/141‑1 (MQ=255)
                                                                                                                      tacgtggtATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTa                     <  4:393166/138‑1 (MQ=255)
                                                                                                                      tAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTa                     <  1:580893/141‑1 (MQ=255)
                                                                                                                        ggtggtATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTACTTGAAACATTATATGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTaaa                   >  1:1081453/1‑141 (MQ=255)
                                                                                                                        ggtggtATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTaaa                   >  2:463307/1‑141 (MQ=255)
                                                                                                                        ggtggtATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTaaa                   >  1:627073/1‑141 (MQ=255)
                                                                                                                        ggtggtATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTaaa                   >  3:450298/1‑141 (MQ=255)
                                                                                                                          tggtATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATGAATTTTTATGGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGATAGCTTTAAATTGACTGGAAAGCGTATATTTAAAAt                 >  1:296487/1‑141 (MQ=255)
                                                                                                                            gtATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTAAAATGa               >  1:539387/1‑141 (MQ=255)
                                                                                                                               tatTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTAAAATGAGTc            >  1:832754/1‑141 (MQ=255)
                                                                                                                                 tttGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTAAAATGAGTCCg          <  1:103350/141‑1 (MQ=255)
                                                                                                                                   tGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTAAAATGAGTCCGAt        >  4:278832/1‑141 (MQ=255)
                                                                                                                                   tGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTAAAATGAGTCCGAt        >  1:920942/1‑141 (MQ=255)
                                                                                                                                         cATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTAAAATGAGTCCGATtcatca  >  4:273496/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                            
TTAGGAGAAACGGCAATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATTAACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCTACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTAATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATATTTAAAATGAGTCCGATTCATCA  >  USA300TCH1516_ALE/1190879‑1191156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: