Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,315,711 T→A 7.0% I331I (ATT→ATA korA → 2‑oxoglutarate oxidoreductase subunit KorA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,315,7110TA 7.0% 357.5 / 13.3 110I331I (ATT→ATAkorA2‑oxoglutarate oxidoreductase subunit KorA
Reads supporting (aligned to +/- strand):  ref base T (61/41);  new base A (6/2);  total (67/43)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

GGATTATCTGGTATGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAG  >  USA300TCH1516_ALE/1315571‑1315850
                                                                                                                                            |                                                                                                                                           
ggATTATCTGGTATGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAAtt                                                                                                                                             <  4:443576/141‑1 (MQ=255)
 gATTATCTGGTATGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAAttg                                                                                                                                            <  2:1017964/141‑1 (MQ=255)
 gATTATCTGGTATGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAAttg                                                                                                                                            >  4:259860/1‑141 (MQ=255)
   ttATCTGGTATGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAAttgtt                                                                                                                                          <  4:368140/141‑1 (MQ=255)
   ttATCTGGTATGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAAttgtt                                                                                                                                          <  4:415818/141‑1 (MQ=255)
     aTCTGGTATGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATtgttgt                                                                                                                                        <  2:899775/141‑1 (MQ=255)
      tCTGGTATGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTa                                                                                                                                       >  4:13294/1‑141 (MQ=255)
        tGGTATGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGc                                                                                                                                     <  4:20540/141‑1 (MQ=255)
        tGGTATGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGc                                                                                                                                     <  1:343257/141‑1 (MQ=255)
          gTATGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCAc                                                                                                                                   <  1:255661/141‑1 (MQ=255)
            aTGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCa                                                                                                                                 >  3:14641/1‑141 (MQ=255)
             tGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCaa                                                                                                                                <  2:885321/141‑1 (MQ=255)
             tGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCaa                                                                                                                                >  1:187430/1‑141 (MQ=255)
               aCTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACa                                                                                                                              >  2:912036/1‑141 (MQ=255)
                cTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAg                                                                                                                             >  2:99822/1‑141 (MQ=255)
                  gAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGAt                                                                                                                           <  1:633101/141‑1 (MQ=255)
                   aaaCGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATg                                                                                                                          >  1:339092/1‑141 (MQ=255)
                   aaaCGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATg                                                                                                                          >  4:254920/1‑141 (MQ=255)
                     aCGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCa                                                                                                                        >  4:396807/1‑141 (MQ=255)
                           ttAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGAt                                                                                                                  >  1:116784/1‑141 (MQ=255)
                            tAGTCATTATTAATACTCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATg                                                                                                                 >  3:177445/1‑141 (MQ=255)
                            tAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATg                                                                                                                 <  1:163733/141‑1 (MQ=255)
                              gTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCa                                                                                                               >  3:81066/1‑141 (MQ=255)
                                  ttattaATACTCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCAtttt                                                                                                           >  2:342097/1‑141 (MQ=255)
                                    attaATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATtttat                                                                                                         <  1:187485/141‑1 (MQ=255)
                                         tACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAAc                                                                                                    <  1:201070/141‑1 (MQ=255)
                                             cAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATg                                                                                                >  1:1074105/1‑141 (MQ=255)
                                               aCGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGa                                                                                              >  2:701424/1‑141 (MQ=255)
                                               aCGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGa                                                                                              <  1:77314/141‑1 (MQ=255)
                                                 gAGGTGGAACTTCTACTGGATTACGTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGATGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAg                                                                                            <  4:397146/141‑1 (MQ=255)
                                                  aGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGc                                                                                           >  1:737431/1‑141 (MQ=255)
                                                  aGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGc                                                                                           <  4:241714/141‑1 (MQ=255)
                                                      tgatcTTTTACTTGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCattt                                                                                       >  2:74820/5‑141 (MQ=255)
                                                      ggACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCattt                                                                                       >  1:1026468/1‑141 (MQ=255)
                                                      ggACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCattt                                                                                       >  1:122941/1‑141 (MQ=255)
                                                       gACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCattta                                                                                      >  1:708546/1‑141 (MQ=255)
                                                         ccTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCatttaat                                                                                    <  1:941075/141‑1 (MQ=255)
                                                         ccTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGGAGATGCATTTTATTTAACTATGGAAGCctttaat                                                                                    >  4:194856/1‑141 (MQ=255)
                                                          cTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCatttaatt                                                                                   <  3:1487/141‑1 (MQ=255)
                                                          cTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGAAGAAGATGCATTTTATTTAACTATGGAAGCagttaatt                                                                                   >  2:112438/1‑141 (MQ=255)
                                                             cTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAg                                                                                <  2:124239/141‑1 (MQ=255)
                                                                    aTTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACa                                                                         >  1:693828/1‑141 (MQ=255)
                                                                        ccTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAatat                                                                     >  1:236664/1‑141 (MQ=255)
                                                                          tACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCa                                                                   >  2:428105/1‑141 (MQ=255)
                                                                            cGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTGATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAAt                                                                 >  2:1039366/1‑141 (MQ=255)
                                                                            cGAAACAAGAACAGTCAGATTTAATGCAAATGATTGATTGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAAt                                                                 <  1:342036/141‑1 (MQ=255)
                                                                              aaaCAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGc                                                               <  1:455065/141‑1 (MQ=255)
                                                                                 cAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATACCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCt                                                            <  2:992363/141‑1 (MQ=255)
                                                                                  aaGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTg                                                           >  1:863082/1‑141 (MQ=255)
                                                                                  aaGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTg                                                           >  1:115710/1‑141 (MQ=255)
                                                                                   aGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGcccaat                                                          >  1:546548/1‑138 (MQ=255)
                                                                                   aGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGt                                                          >  4:38581/1‑141 (MQ=255)
                                                                                   aGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGt                                                          <  4:247085/141‑1 (MQ=255)
                                                                                   aGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGt                                                          >  1:863073/1‑141 (MQ=255)
                                                                                    gAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGtt                                                         >  3:285360/1‑141 (MQ=255)
                                                                                    gAACAGTCAGATTTAATGCAAATGATATATGGTACACAAGGGGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTgat                                                         >  2:1058173/1‑139 (MQ=255)
                                                                                        aGTCAGATTTAATGCAAATGATTTATGGTACACAAGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAg                                                     <  2:89940/141‑1 (MQ=255)
                                                                                        aGTCAGATTTAATGCAAATGATTTAGGGTACACGAGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAg                                                     <  2:983499/141‑1 (MQ=255)
                                                                                         gTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGt                                                    <  4:449870/141‑1 (MQ=255)
                                                                                          tCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGtt                                                   <  2:791397/141‑1 (MQ=255)
                                                                                            aGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCt                                                 <  2:518519/141‑1 (MQ=255)
                                                                                            aGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGtttt                                                 <  3:430247/141‑3 (MQ=255)
                                                                                             gATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTa                                                >  1:1049628/1‑141 (MQ=255)
                                                                                             gATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGtttta                                                <  2:555809/141‑4 (MQ=255)
                                                                                             gATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTTTa                                                >  4:304044/1‑141 (MQ=255)
                                                                                             aaTTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTa                                                <  2:234276/140‑1 (MQ=255)
                                                                                                ttAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGt                                             <  1:516025/141‑1 (MQ=255)
                                                                                                ttAATGCAAATGATTTATGGTACACATAGAGAAAAAAAAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGt                                             <  2:406984/141‑1 (MQ=255)
                                                                                                  aaTGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGa                                           >  3:148287/1‑141 (MQ=255)
                                                                                                    tGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGAtt                                         <  1:291573/141‑1 (MQ=255)
                                                                                                     gCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGAttt                                        >  2:489954/1‑141 (MQ=255)
                                                                                                     gCAAATGATTTATAGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGAttt                                        <  4:281456/141‑1 (MQ=255)
                                                                                                       aaaTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGc                                      >  1:708439/1‑141 (MQ=255)
                                                                                                        aaTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCa                                     <  2:595032/141‑1 (MQ=255)
                                                                                                         aTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCaa                                    <  2:617741/141‑1 (MQ=255)
                                                                                                         aTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCaa                                    >  2:99457/1‑141 (MQ=255)
                                                                                                            aTTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTa                                 <  1:543867/141‑1 (MQ=255)
                                                                                                            aTTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTa                                 <  1:543862/141‑1 (MQ=255)
                                                                                                             tttATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTAt                                >  1:522935/1‑141 (MQ=255)
                                                                                                              ttATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATc                               >  3:280094/1‑141 (MQ=255)
                                                                                                                aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                             >  3:227245/1‑141 (MQ=255)
                                                                                                                aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                             >  2:917326/1‑141 (MQ=255)
                                                                                                                aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                             <  1:806693/141‑1 (MQ=255)
                                                                                                                 tGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCttt                            >  2:848184/1‑141 (MQ=255)
                                                                                                                    tACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAgg                         <  2:891681/141‑1 (MQ=255)
                                                                                                                    tACACATGGTGATATTCCAAAAATTGTTGTAAACACGACATATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAgg                         >  1:29959/1‑141 (MQ=255)
                                                                                                                       acaTGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaa                      <  2:252249/141‑1 (MQ=255)
                                                                                                                          tGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaaca                   >  2:488376/1‑141 (MQ=255)
                                                                                                                          tGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaaca                   >  2:887063/1‑141 (MQ=255)
                                                                                                                             tGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCAtt                                                                                                             <  1:674425/48‑1 (MQ=255)
                                                                                                                             tGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCAtt                                                                                                             >  2:674425/1‑48 (MQ=255)
                                                                                                                             tGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaacaaac                >  3:367160/1‑141 (MQ=255)
                                                                                                                             tGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaacaaac                >  3:105840/1‑141 (MQ=255)
                                                                                                                                tatTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGt             >  3:286662/1‑141 (MQ=255)
                                                                                                                                  ttCCAAAAAATGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTg           >  1:956513/1‑141 (MQ=255)
                                                                                                                                   tCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGa          <  2:449140/141‑1 (MQ=255)
                                                                                                                                      aaaaaTTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGaaaa       >  2:13216/1‑141 (MQ=255)
                                                                                                                                      aaaaaTTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGaaaa       >  2:767645/1‑141 (MQ=255)
                                                                                                                                       aaaaTTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGaaaaa      >  1:569208/1‑141 (MQ=255)
                                                                                                                                           ttgttgTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAg  <  3:258240/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GGATTATCTGGTATGACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAG  >  USA300TCH1516_ALE/1315571‑1315850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: