Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,315,726 A→T 6.8% T336T (ACA→ACT korA → 2‑oxoglutarate oxidoreductase subunit KorA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,315,7260AT 6.8% 380.1 / 12.7 118T336T (ACA→ACTkorA2‑oxoglutarate oxidoreductase subunit KorA
Reads supporting (aligned to +/- strand):  ref base A (70/40);  new base T (6/2);  total (76/42)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 119 total aligned reads displayed.

ACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTG  >  USA300TCH1516_ALE/1315586‑1315865
                                                                                                                                            |                                                                                                                                           
aCTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACa                                                                                                                                             >  2:912036/1‑141 (MQ=255)
 cTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAg                                                                                                                                            >  2:99822/1‑141 (MQ=255)
    aaaCGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATg                                                                                                                                         >  4:254920/1‑141 (MQ=255)
    aaaCGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATg                                                                                                                                         >  4:249540/1‑141 (MQ=255)
    aaaCGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATg                                                                                                                                         >  1:339092/1‑141 (MQ=255)
      aCGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCa                                                                                                                                       >  4:396807/1‑141 (MQ=255)
            ttAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGAt                                                                                                                                 >  1:116784/1‑141 (MQ=255)
             tAGTCATTATTAATACTCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATg                                                                                                                                >  3:177445/1‑141 (MQ=255)
             tAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTAGACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATg                                                                                                                                >  4:391252/1‑141 (MQ=255)
             tAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATg                                                                                                                                <  1:163733/141‑1 (MQ=255)
               gTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCa                                                                                                                              >  3:81066/1‑141 (MQ=255)
                tCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCAt                                                                                                                             <  4:450788/141‑1 (MQ=255)
                   ttattaATACTCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCAtttt                                                                                                                          >  2:342097/1‑141 (MQ=255)
                     attaATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATtttat                                                                                                                        <  1:187485/141‑1 (MQ=255)
                          tACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAAc                                                                                                                   <  1:201070/141‑1 (MQ=255)
                              cAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATg                                                                                                               >  1:1074105/1‑141 (MQ=255)
                                aCGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGa                                                                                                             >  2:701424/1‑141 (MQ=255)
                                aCGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGa                                                                                                             <  1:77314/141‑1 (MQ=255)
                                  gAGGTGGAACTTCTACTGGATTACGTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGATGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAg                                                                                                           <  4:397146/141‑1 (MQ=255)
                                   aGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGc                                                                                                          >  1:737431/1‑141 (MQ=255)
                                   aGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGc                                                                                                          <  4:241714/141‑1 (MQ=255)
                                    ggTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCa                                                                                                         <  2:90399/141‑1 (MQ=255)
                                       ggACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCattt                                                                                                      >  1:122941/1‑141 (MQ=255)
                                       ggACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCattt                                                                                                      >  1:1026468/1‑141 (MQ=255)
                                        gACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCattta                                                                                                     >  1:708546/1‑141 (MQ=255)
                                        gACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATGTATGGTACACATGGTGATATTCCAAAAATTGTTGTATCACCAACAGATGCAGAAGATGCATTTTATTTAACGATGGaatgataaa                                                                                                     >  1:500311/1‑134 (MQ=255)
                                          ccTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCatttaat                                                                                                   <  1:941075/141‑1 (MQ=255)
                                          ccTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGGAGATGCATTTTATTTAACTATGGAAGCctttaat                                                                                                   >  4:194856/1‑141 (MQ=255)
                                           cTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCatttaatt                                                                                                  <  3:1487/141‑1 (MQ=255)
                                           cTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGAAGAAGATGCATTTTATTTAACTATGGAAGCagttaatt                                                                                                  >  2:112438/1‑141 (MQ=255)
                                              cTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAg                                                                                               <  2:124239/141‑1 (MQ=255)
                                                     aTTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACa                                                                                        >  1:693828/1‑141 (MQ=255)
                                                         ccTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAatat                                                                                    >  1:236664/1‑141 (MQ=255)
                                                           tACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCa                                                                                  >  2:428105/1‑141 (MQ=255)
                                                             cGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTGATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAAt                                                                                >  2:1039366/1‑141 (MQ=255)
                                                             cGAAACAAGAACAGTCAGATTTAATGCAAATGATTGATTGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAAt                                                                                <  1:342036/141‑1 (MQ=255)
                                                               aaaCAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGc                                                                              <  1:455065/141‑1 (MQ=255)
                                                                  cAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATACCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCt                                                                           <  2:992363/141‑1 (MQ=255)
                                                                   aaGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTg                                                                          >  1:115710/1‑141 (MQ=255)
                                                                   aaGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTg                                                                          >  1:863082/1‑141 (MQ=255)
                                                                    aGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGcccaat                                                                         >  1:546548/1‑138 (MQ=255)
                                                                    aGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGt                                                                         >  1:863073/1‑141 (MQ=255)
                                                                     gAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGtt                                                                        >  3:285360/1‑141 (MQ=255)
                                                                     gAACAGTCAGATTTAATGCAAATGATATATGGTACACAAGGGGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTgat                                                                        >  2:1058173/1‑139 (MQ=255)
                                                                         aGTCAGATTTAATGCAAATGATTTATGGTACACAAGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAg                                                                    <  2:89940/141‑1 (MQ=255)
                                                                          gTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGt                                                                   <  4:449870/141‑1 (MQ=255)
                                                                           tCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGtt                                                                  <  2:791397/141‑1 (MQ=255)
                                                                             aGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCt                                                                <  2:518519/141‑1 (MQ=255)
                                                                             aGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGtttt                                                                <  3:430247/141‑3 (MQ=255)
                                                                              gATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTa                                                               >  1:1049628/1‑141 (MQ=255)
                                                                              gATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGtttta                                                               <  2:555809/141‑4 (MQ=255)
                                                                              gATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTTTa                                                               >  4:304044/1‑141 (MQ=255)
                                                                              aaTTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTa                                                               <  2:234276/140‑1 (MQ=255)
                                                                                   aaTGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGa                                                          >  3:148287/1‑141 (MQ=255)
                                                                                      gCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGAttt                                                       >  1:290574/1‑141 (MQ=255)
                                                                                      gCAAATGATTTATAGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGAttt                                                       <  4:281456/141‑1 (MQ=255)
                                                                                       cAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAANTGATTTg                                                      >  2:813906/1‑141 (MQ=255)
                                                                                        aaaTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGc                                                     >  1:708439/1‑141 (MQ=255)
                                                                                         aaTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCa                                                    <  2:595032/141‑1 (MQ=255)
                                                                                          aTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCaa                                                   <  2:617741/141‑1 (MQ=255)
                                                                                          aTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCaa                                                   >  2:99457/1‑141 (MQ=255)
                                                                                           tGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAAt                                                  <  3:25557/141‑1 (MQ=255)
                                                                                            gATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGACGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAAtt                                                 >  2:1000374/1‑141 (MQ=255)
                                                                                             aTTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTa                                                >  2:467539/1‑141 (MQ=255)
                                                                                             aTTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTa                                                <  1:543867/141‑1 (MQ=255)
                                                                                             aTTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTa                                                <  1:543862/141‑1 (MQ=255)
                                                                                              tttATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTAt                                               >  1:522935/1‑141 (MQ=255)
                                                                                               ttATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATc                                              >  3:280094/1‑141 (MQ=255)
                                                                                                 aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                            >  2:917326/1‑141 (MQ=255)
                                                                                                 aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                            >  3:72414/1‑141 (MQ=255)
                                                                                                 aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                            >  1:62746/1‑141 (MQ=255)
                                                                                                 aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                            <  1:806693/141‑1 (MQ=255)
                                                                                                  tGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCttt                                           >  2:848184/1‑141 (MQ=255)
                                                                                                     tACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAgg                                        <  2:891681/141‑1 (MQ=255)
                                                                                                     tACACATGGTGATATTCCAAAAATTGTTGTAAACACGACATATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAgg                                        >  1:29959/1‑141 (MQ=255)
                                                                                                        acaTGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaa                                     <  2:252249/141‑1 (MQ=255)
                                                                                                           tGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaaca                                  >  2:488376/1‑141 (MQ=255)
                                                                                                              tGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCAtt                                                                                                                            <  1:674425/48‑1 (MQ=255)
                                                                                                              tGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCAtt                                                                                                                            >  2:674425/1‑48 (MQ=255)
                                                                                                              tGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaacaaac                               >  3:105840/1‑141 (MQ=255)
                                                                                                              tGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaacaaac                               >  3:105818/1‑141 (MQ=255)
                                                                                                               gATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACt                              >  2:176378/1‑141 (MQ=255)
                                                                                                                 tatTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGt                            >  3:286662/1‑141 (MQ=255)
                                                                                                                    tCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGa                         <  2:449140/141‑1 (MQ=255)
                                                                                                                       aaaaaTTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGaaaa                      >  2:767645/1‑141 (MQ=255)
                                                                                                                        aaaaTTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGaaaaa                     >  1:569208/1‑141 (MQ=255)
                                                                                                                            ttgttgTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAg                 <  3:258240/141‑1 (MQ=255)
                                                                                                                               ttgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGAtt              >  2:49829/1‑141 (MQ=255)
                                                                                                                               ttgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGAtt              <  3:176745/141‑1 (MQ=255)
                                                                                                                               ttgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGAtt              >  2:377769/1‑141 (MQ=255)
                                                                                                                               ttgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGAtt              >  2:489442/1‑141 (MQ=255)
                                                                                                                                tgtAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTTTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATta             >  2:822190/1‑141 (MQ=255)
                                                                                                                                tgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATta             >  3:296468/1‑141 (MQ=255)
                                                                                                                                tgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATta             >  2:629588/1‑141 (MQ=255)
                                                                                                                                tgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATta             >  2:707619/1‑141 (MQ=255)
                                                                                                                                 gtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGTTtat            >  3:169421/1‑141 (MQ=255)
                                                                                                                                 gtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATtat            >  2:172169/1‑141 (MQ=255)
                                                                                                                                         aaCAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTAt    >  2:565384/1‑141 (MQ=255)
                                                                                                                                          aCAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTAtt   >  4:337128/1‑141 (MQ=255)
                                                                                                                                          aCAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTAtt   >  2:837146/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
ACTGAAACGCCATTAGTCATTATTAATACCCAACGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTG  >  USA300TCH1516_ALE/1315586‑1315865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: