Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,315,762 A→C 7.5% A348A (GCA→GCC korA → 2‑oxoglutarate oxidoreductase subunit KorA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,315,7620AC 7.5% 325.8 / 15.9 107A348A (GCA→GCCkorA2‑oxoglutarate oxidoreductase subunit KorA
Reads supporting (aligned to +/- strand):  ref base A (63/36);  new base C (5/3);  total (68/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 118 total aligned reads displayed.

GGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGA  >  USA300TCH1516_ALE/1315622‑1315902
                                                                                                                                            |                                                                                                                                            
ggTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCa                                                                                                                                              <  2:90399/141‑1 (MQ=255)
   tgatcTTTTACTTGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCattt                                                                                                                                           >  2:74820/5‑141 (MQ=255)
   ggACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCattt                                                                                                                                           >  1:1026468/1‑141 (MQ=255)
   ggACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCattt                                                                                                                                           >  1:122941/1‑141 (MQ=255)
    gACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCattta                                                                                                                                          >  1:708546/1‑141 (MQ=255)
      ccTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCatttaat                                                                                                                                        <  1:941075/141‑1 (MQ=255)
      ccTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGGAGATGCATTTTATTTAACTATGGAAGCctttaat                                                                                                                                        >  4:194856/1‑141 (MQ=255)
       cTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGAAGAAGATGCATTTTATTTAACTATGGAAGCagttaatt                                                                                                                                       >  2:112438/1‑141 (MQ=255)
          cTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAg                                                                                                                                    <  2:124239/141‑1 (MQ=255)
                     ccTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAatat                                                                                                                         >  1:236664/1‑141 (MQ=255)
                       tACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCa                                                                                                                       >  2:428105/1‑141 (MQ=255)
                           aaaCAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGc                                                                                                                   <  1:455065/141‑1 (MQ=255)
                              cAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATACCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCt                                                                                                                <  2:992363/141‑1 (MQ=255)
                               aaGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTg                                                                                                               >  1:863082/1‑141 (MQ=255)
                                aGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGcccaat                                                                                                              >  1:546548/1‑138 (MQ=255)
                                aGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGt                                                                                                              >  4:38581/1‑141 (MQ=255)
                                 gAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGtt                                                                                                             >  3:285360/1‑141 (MQ=255)
                                 gAACAGTCAGATTTAATGCAAATGATATATGGTACACAAGGGGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTgat                                                                                                             >  2:1058173/1‑139 (MQ=255)
                                     aGTCAGATTTAATGCAAATGATTTAGGGTACACGAGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAg                                                                                                         <  2:983499/141‑1 (MQ=255)
                                      gTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGt                                                                                                        <  4:449870/141‑1 (MQ=255)
                                       tCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGtt                                                                                                       <  2:791397/141‑1 (MQ=255)
                                         aGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCt                                                                                                     <  2:518519/141‑1 (MQ=255)
                                         aGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGtttt                                                                                                     <  3:430247/141‑3 (MQ=255)
                                          gATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTa                                                                                                    >  1:1049628/1‑141 (MQ=255)
                                          gATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGtttta                                                                                                    <  2:555809/141‑4 (MQ=255)
                                          gATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTTTa                                                                                                    >  4:304044/1‑141 (MQ=255)
                                          aaTTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTa                                                                                                    <  2:234276/140‑1 (MQ=255)
                                             ttAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGt                                                                                                 <  1:516025/141‑1 (MQ=255)
                                             ttAATGCAAATGATTTATGGTACACATAGAGAAAAAAAAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGt                                                                                                 <  2:406984/141‑1 (MQ=255)
                                               aaTGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGa                                                                                               >  3:148287/1‑141 (MQ=255)
                                                 tGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGAtt                                                                                             <  1:291573/141‑1 (MQ=255)
                                                  gCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGAttt                                                                                            >  1:290574/1‑141 (MQ=255)
                                                  gCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGAttt                                                                                            >  2:489954/1‑141 (MQ=255)
                                                  gCAAATGATTTATAGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGAttt                                                                                            <  4:281456/141‑1 (MQ=255)
                                                   cAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAANTGATTTg                                                                                           >  2:813906/1‑141 (MQ=255)
                                                    aaaTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGc                                                                                          >  1:708439/1‑141 (MQ=255)
                                                     aaTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCa                                                                                         <  2:595032/141‑1 (MQ=255)
                                                      aTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCaa                                                                                        >  2:608128/1‑141 (MQ=255)
                                                      aTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCaa                                                                                        >  2:99457/1‑141 (MQ=255)
                                                      aTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCaa                                                                                        <  2:617741/141‑1 (MQ=255)
                                                       tGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAAt                                                                                       <  3:25557/141‑1 (MQ=255)
                                                        gATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGACGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAAtt                                                                                      >  2:1000374/1‑141 (MQ=255)
                                                         aTTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTa                                                                                     <  1:543867/141‑1 (MQ=255)
                                                         aTTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTa                                                                                     >  2:467539/1‑141 (MQ=255)
                                                         aTTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTa                                                                                     <  1:543862/141‑1 (MQ=255)
                                                           ttATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATc                                                                                   >  3:280094/1‑141 (MQ=255)
                                                             aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                                                                 >  2:917326/1‑141 (MQ=255)
                                                             aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                                                                 >  1:62746/1‑141 (MQ=255)
                                                             aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                                                                 <  1:806693/141‑1 (MQ=255)
                                                             aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                                                                 >  3:227245/1‑141 (MQ=255)
                                                             aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                                                                 >  3:72414/1‑141 (MQ=255)
                                                              tGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCttt                                                                                >  2:848184/1‑141 (MQ=255)
                                                                 tACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAgg                                                                             <  2:891681/141‑1 (MQ=255)
                                                                 tACACATGGTGATATTCCAAAAATTGTTGTAAACACGACATATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAgg                                                                             >  1:29959/1‑141 (MQ=255)
                                                                    acaTGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaa                                                                          <  2:252249/141‑1 (MQ=255)
                                                                       tGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaaca                                                                       >  2:488376/1‑141 (MQ=255)
                                                                       tGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaaca                                                                       >  2:887063/1‑141 (MQ=255)
                                                                          tGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaacaaac                                                                    >  3:105840/1‑141 (MQ=255)
                                                                           gATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACt                                                                   >  2:176378/1‑141 (MQ=255)
                                                                             tatTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGt                                                                 >  3:286662/1‑141 (MQ=255)
                                                                               ttCCAAAAAATGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTg                                                               >  1:956513/1‑141 (MQ=255)
                                                                                   aaaaaTTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGaaaa                                                           >  2:13216/1‑141 (MQ=255)
                                                                                   aaaaaTTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGaaaa                                                           >  2:767645/1‑141 (MQ=255)
                                                                                    aaaaTTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGaaaaa                                                          >  1:569208/1‑141 (MQ=255)
                                                                                       aTTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTa                                                       <  1:770076/141‑1 (MQ=255)
                                                                                           ttgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGAtt                                                   >  2:489442/1‑141 (MQ=255)
                                                                                           ttgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGAtt                                                   <  3:176745/141‑1 (MQ=255)
                                                                                           ttgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGAtt                                                   >  2:377769/1‑141 (MQ=255)
                                                                                            tgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATta                                                  >  3:296468/1‑141 (MQ=255)
                                                                                            tgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATta                                                  >  2:707619/1‑141 (MQ=255)
                                                                                             gtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGTTtat                                                 >  3:169421/1‑141 (MQ=255)
                                                                                                     aaCAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTAt                                         >  2:565384/1‑141 (MQ=255)
                                                                                                      aCAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTAtt                                        >  2:837146/1‑141 (MQ=255)
                                                                                                      aCAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTAtt                                        >  4:337128/1‑141 (MQ=255)
                                                                                                       cAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTg                                       >  3:111119/1‑141 (MQ=255)
                                                                                                          aTGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATACCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTCAACAAACTGTTGAAAAATTAGATTATAATCGTATTGaaa                                    >  2:1066853/1‑141 (MQ=255)
                                                                                                           tGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAAt                                   <  1:435826/141‑1 (MQ=255)
                                                                                                             cAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTa                                 >  1:111159/1‑141 (MQ=255)
                                                                                                                aagaTGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAAc                              <  3:13294/141‑1 (MQ=255)
                                                                                                                    tGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACgtgg                          >  3:80883/1‑141 (MQ=255)
                                                                                                                     gCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACgtggt                         <  3:146588/141‑1 (MQ=255)
                                                                                                                      cATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACgtggtg                        >  1:737850/1‑141 (MQ=255)
                                                                                                                       aTTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGa                       >  1:123379/1‑141 (MQ=255)
                                                                                                                       aTTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGa                       <  2:708546/141‑1 (MQ=255)
                                                                                                                           tatttaACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATc                   >  4:113045/1‑141 (MQ=255)
                                                                                                                             tttaACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCAt                 <  2:938611/141‑1 (MQ=255)
                                                                                                                             tttaACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCAt                 >  3:237674/1‑141 (MQ=255)
                                                                                                                                aaCTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCa              >  2:486411/1‑141 (MQ=255)
                                                                                                                                 aCTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTTTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGCATTg                                       <  1:1016607/115‑1 (MQ=255)
                                                                                                                                 aCTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTTTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGCATTg                                       >  2:1016607/1‑115 (MQ=255)
                                                                                                                                 aCTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCaa             >  3:241745/1‑141 (MQ=255)
                                                                                                                                 aCTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCaa             <  4:14641/141‑1 (MQ=255)
                                                                                                                                 aCTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCaa             >  1:1087026/1‑141 (MQ=255)
                                                                                                                                     tGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTg         <  2:868785/141‑1 (MQ=255)
                                                                                                                                       gAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTTTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGCATTGAAATTAAACGAGGTGAAATCATTCAATCTGat       >  1:502200/1‑141 (MQ=255)
                                                                                                                                       gAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGat       >  2:102015/1‑141 (MQ=255)
                                                                                                                                       gAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTACTGAAATTAAACGTGGTGAAATCATTCAATCTGat       >  1:1003880/1‑141 (MQ=255)
                                                                                                                                         tgtatttattttaGTATAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGTAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATATTCGTATTGAAATTAAACGTTGTGAAATCATTCAATCTGatat     <  1:610527/138‑1 (MQ=255)
                                                                                                                                         aGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGatat     <  3:284211/141‑1 (MQ=255)
                                                                                                                                            atttaatttaGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGa  <  1:377769/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGA  >  USA300TCH1516_ALE/1315622‑1315902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: