Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 70,101 A→T 5.0% I228N (ATT→AAT)  argF_1 ← Ornithine carbamoyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE70,1010AT 5.0% 445.9 / 11.9 140I228N (ATT→AAT) argF_1Ornithine carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base A (61/72);  new base T (3/4);  total (64/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

Only 100 of 141 total aligned reads displayed.

AGCAATGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGATGGAAAGT  >  USA300TCH1516_ALE/69964‑70240
                                                                                                                                          |                                                                                                                                           
aGCAATGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTa                                                                                                                                          <  4:137744/141‑1 (MQ=255)
    aagTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGTTAAGGTTCTAATAATTTGATACGTTTTTCCCAAACGTCATCAGGTTCACCCATAGATACCCAAACATCTGATGTAATTTACac                                                                                                                                       <  2:922607/139‑2 (MQ=255)
    aTGTTCAACAATTGTATGCGGATCACATCTTTTTCTGCTTCAATTTTTTCTTACTCGGTATGTTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATc                                                                                                                                      <  1:1024855/141‑1 (MQ=21)
    aTGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATNGATACCCAAACANCTGTGTAAATTACATc                                                                                                                                      <  3:311517/141‑1 (MQ=255)
    aTGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGATGTAATTTACac                                                                                                                                       <  2:801425/141‑2 (MQ=255)
    aTGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGATGTAATTTACac                                                                                                                                       <  2:801245/141‑2 (MQ=255)
    aTGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGATGTAATTTACac                                                                                                                                       <  2:756828/141‑2 (MQ=255)
    aTGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATc                                                                                                                                      <  1:113073/141‑1 (MQ=255)
    aTGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATc                                                                                                                                      <  3:18107/141‑1 (MQ=255)
        tCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGAt                                                                                                                                  >  1:45352/1‑141 (MQ=255)
         cAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATc                                                                                                                                 <  1:395196/141‑1 (MQ=255)
           aaaaTTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCt                                                                                                                               <  3:126235/141‑1 (MQ=255)
            aaaTTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCtt                                                                                                                              >  4:430037/1‑141 (MQ=255)
             aaTTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCttt                                                                                                                             <  4:75381/141‑1 (MQ=255)
              aTTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTg                                                                                                                            >  1:114391/1‑141 (MQ=255)
                tGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGac                                                                                                                          <  2:771716/141‑1 (MQ=255)
                tGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGac                                                                                                                          >  2:129927/1‑141 (MQ=255)
                tGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGac                                                                                                                          >  4:246322/1‑141 (MQ=255)
                  tATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGacac                                                                                                                        <  2:167638/141‑1 (MQ=255)
                   aTGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACAcc                                                                                                                       >  3:431847/1‑141 (MQ=255)
                   aTGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACAcc                                                                                                                       >  1:141502/1‑141 (MQ=255)
                          ttACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTtcatca                                                                                                                <  1:178913/141‑1 (MQ=255)
                                gTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATatca                                                                                                          >  3:193022/1‑141 (MQ=255)
                                 tttttttCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATatcat                                                                                                         >  1:267287/1‑141 (MQ=255)
                                       tCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTTTTTTTTTTTTTCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACATCTTCATCAATATCATCTGTta                                                                                                   >  2:2745/1‑141 (MQ=255)
                                        cATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTtat                                                                                                  >  1:946915/1‑141 (MQ=255)
                                            aaTTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAgg                                                                                              <  2:721304/141‑1 (MQ=255)
                                            aaTTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAgg                                                                                              <  4:267318/141‑1 (MQ=255)
                                             aTTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGa                                                                                             <  1:142216/141‑1 (MQ=255)
                                              ttCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTTTTTTTTTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATTAGGAt                                                                                            >  2:449786/1‑141 (MQ=255)
                                              ttCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAggct                                                                                            >  1:469791/1‑139 (MQ=255)
                                                    aGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTcacc                                                                                      <  2:243604/141‑1 (MQ=255)
                                                     gTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTcacca                                                                                     <  1:42326/141‑1 (MQ=255)
                                                      ttACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTcaccac                                                                                    >  3:416375/1‑141 (MQ=255)
                                                       tACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCaccacc                                                                                   >  1:101566/1‑141 (MQ=255)
                                                       tACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCaccacc                                                                                   <  4:394621/141‑1 (MQ=255)
                                                        acacGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCg                                                                                  <  2:601651/141‑1 (MQ=255)
                                                          acGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACAACAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGtt                                                                                <  2:539178/141‑1 (MQ=255)
                                                             gTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCGCCACCGttttt                                                                             <  1:1074672/141‑1 (MQ=255)
                                                             gTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGttttt                                                                             >  4:454984/1‑141 (MQ=255)
                                                             gTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGttttt                                                                             <  3:124128/141‑1 (MQ=255)
                                                               aTGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGt                                                                           <  4:143102/141‑1 (MQ=255)
                                                               aTGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGt                                                                           >  2:246563/1‑141 (MQ=255)
                                                                 ggTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCATCACCGTTTTTGTCa                                                                         >  2:436211/1‑141 (MQ=255)
                                                                   ttCTAATAATTTGCTACTTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGc                                                                       <  1:332776/141‑1 (MQ=255)
                                                                   ttCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGc                                                                       <  2:221320/141‑1 (MQ=255)
                                                                    tCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCt                                                                      <  1:470171/141‑1 (MQ=255)
                                                                    tCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCt                                                                      <  1:1066742/141‑1 (MQ=255)
                                                                     cTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCta                                                                     >  3:332740/1‑141 (MQ=255)
                                                                      taataaTTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCtat                                                                    <  4:402217/141‑1 (MQ=255)
                                                                       aataatTTGATACGTTTTTTTTTTTTTTTTTCAGGTTCACCCATAGATACCCAAACATCTGTGCAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCTTTTTTGTCATCtata                                                                   >  2:513869/1‑141 (MQ=255)
                                                                        ataatTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTatat                                                                  <  2:282451/141‑1 (MQ=255)
                                                                           atTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCAt                                                               >  1:229068/1‑141 (MQ=255)
                                                                            tttGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCAtt                                                              <  3:228605/141‑1 (MQ=255)
                                                                              tGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTac                                                            >  2:708982/1‑141 (MQ=255)
                                                                               gATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTaca                                                           <  1:835602/141‑1 (MQ=255)
                                                                               gATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTaca                                                           >  2:404119/1‑141 (MQ=255)
                                                                                aTACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTacac                                                          >  3:48071/1‑141 (MQ=255)
                                                                                 tACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACAcc                                                         <  1:355487/141‑1 (MQ=255)
                                                                                  aCGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCg                                                        >  2:325584/1‑141 (MQ=255)
                                                                                    gTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGAt                                                      <  2:579452/141‑1 (MQ=255)
                                                                                     tttttCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTAAGCTATATCATTACACCGAtt                                                     >  1:533044/1‑141 (MQ=255)
                                                                                         tCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTtaat                                                 <  2:278736/141‑1 (MQ=255)
                                                                                         tCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTtaat                                                 >  2:294750/1‑141 (MQ=255)
                                                                                         tCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTtaat                                                 <  2:807408/141‑1 (MQ=255)
                                                                                           ccAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTtaataa                                               >  4:277620/1‑141 (MQ=255)
                                                                                             aaaCTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAAtt                                             <  1:3260/141‑1 (MQ=255)
                                                                                              aaCTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTTATCAATATTATCAGTTATAAGGATATCACCACCGTTTTTGTCAGCTATATCATTACACCTTTTTATTAAttt                                            >  4:344840/1‑140 (MQ=255)
                                                                                                   catcaGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTTTTTTTTTTTTCATTACACCGATTGAATAATTCATCAg                                       >  4:381182/1‑141 (MQ=255)
                                                                                                   catcaGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAg                                       <  1:825268/141‑1 (MQ=255)
                                                                                                   catcaGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAg                                       <  2:141502/141‑1 (MQ=255)
                                                                                                   catcaGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAg                                       <  2:141416/141‑1 (MQ=255)
                                                                                                   catcaGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAg                                       <  1:960285/141‑1 (MQ=255)
                                                                                                    atcaGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATCTCATTACACCGATTTAATAATTCATCAGt                                      >  4:307/1‑141 (MQ=255)
                                                                                                    atcaGGTTCACCCATAGATACCCAAACATCAGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATGTAATAATTCATCAGt                                      >  4:386307/1‑141 (MQ=255)
                                                                                                     tcaGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGtt                                     >  2:523938/1‑141 (MQ=255)
                                                                                                     tcaGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGtt                                     >  1:493912/1‑141 (MQ=255)
                                                                                                     tcaGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGtt                                     >  1:51131/1‑141 (MQ=255)
                                                                                                      caGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTg                                    >  2:723690/1‑141 (MQ=255)
                                                                                                       aGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTgg                                   >  4:437680/1‑141 (MQ=255)
                                                                                                          ttCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGAtt                                <  1:594467/141‑1 (MQ=255)
                                                                                                            cACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATGCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTa                              >  4:112968/1‑141 (MQ=255)
                                                                                                             aCCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAg                             <  4:413225/141‑1 (MQ=255)
                                                                                                                  tAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCtt                        <  1:1022847/141‑1 (MQ=255)
                                                                                                                  tAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCtt                        >  1:772205/1‑141 (MQ=255)
                                                                                                                     aTACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAg                     <  3:290515/141‑1 (MQ=255)
                                                                                                                      tACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAgg                    <  1:524229/141‑1 (MQ=255)
                                                                                                                        cccAAATATCTTTGTAAATTACATCATATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGAc                  <  2:93528/141‑1 (MQ=255)
                                                                                                                         ccAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACa                 <  4:158567/141‑1 (MQ=255)
                                                                                                                         ccAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACa                 <  1:736682/141‑1 (MQ=255)
                                                                                                                           aaaCATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATa               >  2:559167/1‑141 (MQ=255)
                                                                                                                             aCATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACa             <  2:362374/141‑1 (MQ=255)
                                                                                                                               aTCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAg           >  1:130796/1‑141 (MQ=255)
                                                                                                                                tctgatgtAATTTACACAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGa          >  1:477118/6‑141 (MQ=255)
                                                                                                                                tCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGa          <  2:587084/141‑1 (MQ=255)
                                                                                                                                 ctgatgtAATTTACACAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGAt         >  1:257326/5‑141 (MQ=255)
                                                                                                                                  tgtgTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGATg        >  1:225661/1‑141 (MQ=255)
                                                                                                                                  tgtgTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAACTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGATg        <  1:844869/141‑1 (MQ=255)
                                                                                                                                  tgtgTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACAACGATCTAATACTTCATCAGTTGGATTTAGTACTTTAGGACATACAAGATg        <  2:377391/141‑1 (MQ=255)
                                                                                                                                         aaTTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGATGGAAAGt  <  1:367602/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AGCAATGTTCAAAAATTGTATGTGGATTACCTGTTTTTTTCATTAATTCTTTAGTTACACGGTATGGTTCTAATAATTTGATACGTTTTTCCCAAACTTCATCAGGTTCACCCATAGATACCCAAACATCTGTGTAAATTACATCAGATCCTTTGACACCTTCATCAATATCATCAGTTATAAGGATTTCACCACCGTTTTTGTCAGCTATATCATTACACCGATTTAATAATTCATCAGTTGGATTTAGTTCTTTAGGACATACAAGATGGAAAGT  >  USA300TCH1516_ALE/69964‑70240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: