Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,315,861 T→C 5.2% R381R (CGT→CGC korA → 2‑oxoglutarate oxidoreductase subunit KorA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,315,8610TC 5.2% 356.9 / 6.8 116R381R (CGT→CGCkorA2‑oxoglutarate oxidoreductase subunit KorA
Reads supporting (aligned to +/- strand):  ref base T (56/53);  new base C (2/4);  total (58/58)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 116 total aligned reads displayed.

AACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAAGGAGGTATTCATC  >  USA300TCH1516_ALE/1315723‑1316000
                                                                                                                                          |                                                                                                                                           
aaCAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTAt                                                                                                                                           >  2:565384/1‑141 (MQ=255)
 aCAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTAtt                                                                                                                                          >  2:837146/1‑141 (MQ=255)
 aCAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTAtt                                                                                                                                          >  4:337128/1‑141 (MQ=255)
  cAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTg                                                                                                                                         >  3:111119/1‑141 (MQ=255)
     aTGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATACCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTCAACAAACTGTTGAAAAATTAGATTATAATCGTATTGaaa                                                                                                                                      >  2:1066853/1‑141 (MQ=255)
      tGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAAt                                                                                                                                     <  1:435826/141‑1 (MQ=255)
        cAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTa                                                                                                                                   >  1:111159/1‑141 (MQ=255)
           aagaTGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAAc                                                                                                                                <  3:13294/141‑1 (MQ=255)
               tGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACgtgg                                                                                                                            >  3:80883/1‑141 (MQ=255)
                gCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACgtggt                                                                                                                           <  3:146588/141‑1 (MQ=255)
                 cATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACgtggtg                                                                                                                          >  1:737850/1‑141 (MQ=255)
                  aTTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGa                                                                                                                         >  1:123379/1‑141 (MQ=255)
                  aTTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGa                                                                                                                         <  2:708546/141‑1 (MQ=255)
                        tttaACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCAt                                                                                                                   >  3:237674/1‑141 (MQ=255)
                        tttaACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCAt                                                                                                                   <  2:938611/141‑1 (MQ=255)
                           aaCTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCa                                                                                                                >  2:486411/1‑141 (MQ=255)
                            aCTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTTTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGCATTg                                                                                                                                         <  1:1016607/115‑1 (MQ=255)
                            aCTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCaa                                                                                                               >  1:1087026/1‑141 (MQ=255)
                            aCTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCaa                                                                                                               >  3:241745/1‑141 (MQ=255)
                            aCTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCaa                                                                                                               <  4:14641/141‑1 (MQ=255)
                                tGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTg                                                                                                           <  2:868785/141‑1 (MQ=255)
                                  gAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGat                                                                                                         >  2:102015/1‑141 (MQ=255)
                                  gAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTACTGAAATTAAACGTGGTGAAATCATTCAATCTGat                                                                                                         >  1:1003880/1‑141 (MQ=255)
                                    tgtatttattttaGTATAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGTAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATATTCGTATTGAAATTAAACGTTGTGAAATCATTCAATCTGatat                                                                                                       <  1:610527/138‑1 (MQ=255)
                                    aacaataaatttaAAAAAACAATATCAATACCCTTATATAGTTATAAGTAATTTGCAATTATCTTTAGGTAAAAAAAAAATAAAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGatat                                                                                                       <  4:416118/134‑1 (MQ=255)
                                    aGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGatat                                                                                                       <  3:284211/141‑1 (MQ=255)
                                        tttaatttaGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGaa                                                                                                   >  3:7613/1‑141 (MQ=255)
                                         ttaatttaGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAAc                                                                                                  >  2:624611/1‑141 (MQ=255)
                                          taatttaGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACg                                                                                                 <  1:629588/141‑1 (MQ=255)
                                           aatttaGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGt                                                                                                >  1:917698/1‑141 (MQ=255)
                                            atttaGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTg                                                                                               <  1:895906/141‑1 (MQ=255)
                                             tttaGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGCTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTga                                                                                              <  1:428105/141‑1 (MQ=255)
                                                 gCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCTATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTgaagaa                                                                                          <  1:854559/141‑1 (MQ=255)
                                                 gCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTgaagaa                                                                                          >  3:433435/1‑141 (MQ=255)
                                                     aaCAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAgatg                                                                                      >  1:64606/1‑141 (MQ=255)
                                                          tatCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATaaa                                                                                 >  1:1048493/1‑141 (MQ=255)
                                                            tCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAgg                                                                               <  4:280094/141‑1 (MQ=255)
                                                            tCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAgg                                                                               >  2:711679/1‑141 (MQ=255)
                                                             cAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGt                                                                              <  4:286662/141‑1 (MQ=255)
                                                                tGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTGAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTAt                                                                           >  1:19084/1‑141 (MQ=255)
                                                                  cccTGTTAAAGTTCAAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTAttt                                                                         <  1:346241/141‑1 (MQ=255)
                                                                   ccTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAGTTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTc                                                                        >  2:30727/1‑141 (MQ=255)
                                                                   ccTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTc                                                                        >  1:267247/1‑141 (MQ=255)
                                                                    cTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCa                                                                       >  2:1020868/1‑141 (MQ=255)
                                                                     tGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAGAGGTTATTTCaa                                                                      <  4:148287/141‑1 (MQ=255)
                                                                       ttATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGc                                                                    <  1:498966/141‑1 (MQ=255)
                                                                       ttATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGc                                                                    <  2:872809/141‑1 (MQ=255)
                                                                        tataGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCg                                                                   <  2:115710/141‑1 (MQ=255)
                                                                         ataGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGt                                                                  <  1:608128/141‑1 (MQ=255)
                                                                         ataGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGt                                                                  >  1:409317/1‑141 (MQ=255)
                                                                            gTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGACAAAGGTTATTTCAAGCGCTAt                                                               >  2:480671/1‑141 (MQ=255)
                                                                              tCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGc                                                             >  2:887943/1‑141 (MQ=255)
                                                                                tAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGt                                                           >  3:453983/1‑141 (MQ=255)
                                                                                   gTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATAGTATTGAAATTAAAAGTGGTGAACTCATTCACTCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTaa                                                        <  1:919268/141‑1 (MQ=255)
                                                                                     gATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGCATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACa                                                      <  1:342097/141‑1 (MQ=255)
                                                                                      aTTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACAt                                                     >  4:69465/1‑141 (MQ=255)
                                                                                         tGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCg                                                  <  1:813906/141‑1 (MQ=255)
                                                                                           cAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGCTTATATTCGTATTGCAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGAt                                                <  4:129013/141‑1 (MQ=255)
                                                                                           cAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGAt                                                <  2:29959/141‑1 (MQ=255)
                                                                                           aaaTTATCTTTAGGTAAAAAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGAt                                                <  1:112438/140‑1 (MQ=255)
                                                                                             aTTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATgg                                              <  1:314519/141‑1 (MQ=255)
                                                                                             aTTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATgg                                              >  3:284834/1‑141 (MQ=255)
                                                                                             aTTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATgg                                              <  1:210555/141‑1 (MQ=255)
                                                                                              ttATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGgt                                             <  2:122941/141‑1 (MQ=255)
                                                                                                 tCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGtt                                          <  1:489954/141‑1 (MQ=255)
                                                                                                  cTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGttt                                         <  1:74820/141‑1 (MQ=255)
                                                                                                  cTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGttt                                         >  2:274223/1‑141 (MQ=255)
                                                                                                    ttAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTtct                                       <  2:116784/141‑1 (MQ=255)
                                                                                                    ttAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTtct                                       <  4:296468/141‑1 (MQ=255)
                                                                                                      aGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTcc                                     <  1:912036/141‑1 (MQ=255)
                                                                                                      aGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTcc                                     >  1:272406/1‑141 (MQ=255)
                                                                                                      aGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTcc                                     >  2:619813/1‑141 (MQ=255)
                                                                                                           aacaaacTGTTGTAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGAc                                <  4:367160/141‑1 (MQ=255)
                                                                                                           aacaaacTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGAc                                >  1:501010/1‑141 (MQ=255)
                                                                                                            acaaacTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGAcc                               >  3:403837/1‑141 (MQ=255)
                                                                                                                 cTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATcc                          >  3:193631/1‑141 (MQ=255)
                                                                                                                 cTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATcc                          >  2:315368/1‑141 (MQ=255)
                                                                                                                   gTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATcccc                        >  4:197087/1‑141 (MQ=255)
                                                                                                                     tGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCgg                      >  1:1050382/1‑141 (MQ=255)
                                                                                                                     tGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCgg                      <  2:236664/141‑1 (MQ=255)
                                                                                                                     tGAAAAATTAGATTATAATCGCATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCAATGGTGTTTCTCCTAGACCTATCCCCgg                      <  4:177445/141‑1 (MQ=255)
                                                                                                                        aaaaTTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGgtgt                   <  1:274223/141‑1 (MQ=255)
                                                                                                                         aaaTTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGtt                  <  3:259860/141‑1 (MQ=255)
                                                                                                                               gATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAagga            >  1:687604/1‑141 (MQ=255)
                                                                                                                               gATTATAATAGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAagga            >  1:954609/1‑141 (MQ=255)
                                                                                                                                aTTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAaggag           <  1:1039366/141‑1 (MQ=255)
                                                                                                                                aTTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAaggag           >  3:20155/1‑141 (MQ=255)
                                                                                                                                aTTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAaggag           >  2:838406/1‑141 (MQ=255)
                                                                                                                                 ttATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAaggagg          <  3:69465/141‑1 (MQ=255)
                                                                                                                                 ttATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAaggagg          >  1:683294/1‑141 (MQ=255)
                                                                                                                                  tataATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAAGGAGGt         >  1:877249/1‑141 (MQ=255)
                                                                                                                                  tataATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAAGGAGGt         >  2:295550/1‑141 (MQ=255)
                                                                                                                                    taATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAAGGAGGTAt       <  3:254920/141‑1 (MQ=255)
                                                                                                                                     aaTCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCATTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAAGGAGGTAtt      >  1:187082/1‑141 (MQ=255)
                                                                                                                                      aTCGTATTGAAATTAAACGTGGTGAACTCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGTCCTATCCCCGGTGTTAAAGGAGGTATtc     <  1:1065938/141‑1 (MQ=255)
                                                                                                                                        cGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAAGGAGGTATtcat   >  3:151824/1‑141 (MQ=255)
                                                                                                                                        cGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAAGGAGGTATtcat   <  1:767645/141‑1 (MQ=255)
                                                                                                                                         gTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAAGGAGGTATtcatc  <  1:701424/141‑1 (MQ=255)
                                                                                                                                         gTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAAGGAGGTATtcatc  >  3:152267/1‑141 (MQ=255)
                                                                                                                                         gTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAAGGAGGTATtcatc  <  1:99822/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCCGGTGTTAAAGGAGGTATTCATC  >  USA300TCH1516_ALE/1315723‑1316000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: