BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,319,300	G→A	5.8%	A478A (GCG→GCA)	miaB →	tRNA‑2‑methylthio‑N(6)‑dimethylallyladenosine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,319,300	0	G	A	5.8%	430.6 / 10.7	137	A478A (GCG→GCA)	miaB	tRNA‑2‑methylthio‑N(6)‑dimethylallyladenosine synthase
Reads supporting (aligned to +/- strand): ref base G (67/62); new base A (2/6); total (69/68)
Fisher's exact test for biased strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01

Only 100 of 142 total aligned reads displayed.

AAGTTGGTCATTATTCAC‑AAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCA  >  USA300TCH1516_ALE/1319161‑1319440

aaGTTGGTCATTATTCAC‑AAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAgcgc                                                                                                                                             <  1:200130/141‑1 (MQ=255)

  gTTGGTCATTATTCACAAAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGcc                                                                                                                                            <  2:518141/141‑1 (MQ=255)

  gTTGGTCATTATTCAC‑AAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAgcgcat                                                                                                                                           >  3:92945/1‑139 (MQ=255)

  gTTGGTCATTATTCAC‑AAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCt                                                                                                                                           >  4:455850/1‑141 (MQ=255)

    tGGTCATTATTCAC‑AAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTaa                                                                                                                                         >  3:232052/1‑141 (MQ=255)

      gTCATTATTCAC‑AAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAg                                                                                                                                       >  2:1071865/1‑141 (MQ=255)

       tCATTATTCAC‑AAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGa                                                                                                                                      >  2:851220/1‑141 (MQ=255)

           tattCAC‑AAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAACAATCCGCTGGTTTTGTTCTATGCGCCTAAAGAAATg                                                                                                                                  <  2:545736/141‑1 (MQ=255)

            attCAC‑AAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGa                                                                                                                                 <  2:438117/141‑1 (MQ=255)

                 c‑aAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGt                                                                                                                            >  1:80889/1‑141 (MQ=255)

                   aaaTAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTa                                                                                                                           >  3:151317/1‑141 (MQ=255)

                   aaaTAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGGTCTTGCGGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTa                                                                                                                           >  1:487096/1‑141 (MQ=255)

                   aaaTAGCTAGGAGGAAGTACGAATGACAAACGGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGGAATGATTGGTa                                                                                                                           <  2:485767/141‑1 (MQ=255)

                      tAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAAc                                                                                                                        >  2:678539/1‑141 (MQ=255)

                      tAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGT‑AAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACt                                                                                                                       <  3:137551/141‑1 (MQ=255)

                        gCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTa                                                                                                                      >  4:431585/1‑141 (MQ=255)

                          tATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGt                                                                                                                    >  3:185848/1‑141 (MQ=255)

                          tATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGt                                                                                                                    <  2:897632/141‑1 (MQ=255)

                            tGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACTCTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTTAACTAGTgg                                                                                                                  <  4:2845/141‑1 (MQ=255)

                            tGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTgg                                                                                                                  >  2:1002824/1‑141 (MQ=255)

                            tGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTgg                                                                                                                  >  3:396227/1‑141 (MQ=255)

                              agtaagtaCGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGaa                                                                                                                >  4:374862/1‑141 (MQ=255)

                              agtaagtaCGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGaa                                                                                                                <  2:30933/141‑1 (MQ=255)

                                taagtaCGAAGGACAAACTGTAACAGTACTTTGTGAAGGTAGTAGTAAAAAAGATGATCAGGTTCTTGCTTGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCACCTAAAGAAATGATTGGTAAACTAGTGGAAGt                                                                                                              <  3:12119/141‑1 (MQ=255)

                                   gtaCGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACg                                                                                                           >  1:321212/1‑141 (MQ=255)

                                    taCGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGa                                                                                                          >  2:714684/1‑141 (MQ=255)

                                     aCGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTCGTGGAAGTACGaa                                                                                                         <  4:108066/141‑1 (MQ=255)

                                     aCGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGaa                                                                                                         <  2:799654/141‑1 (MQ=255)

                                     aCGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGaa                                                                                                         <  4:134858/141‑1 (MQ=255)

                                     aCGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGaa                                                                                                         >  2:1066967/1‑141 (MQ=255)

                                       gAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATa                                                                                                       <  1:71242/141‑1 (MQ=255)

                                            aCAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGa                                                                                                  <  2:95720/141‑1 (MQ=255)

                                             cAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGaa                                                                                                 >  4:412412/1‑141 (MQ=255)

                                              aaaCTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAg                                                                                                <  4:23682/141‑1 (MQ=255)

                                               aaCTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGc                                                                                               >  1:706729/1‑141 (MQ=255)

                                                aCTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCt                                                                                              >  1:850272/1‑141 (MQ=255)

                                                   gTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTaaa                                                                                           >  1:987299/1‑141 (MQ=255)

                                                    tAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAAc                                                                                          <  2:620366/141‑1 (MQ=255)

                                                      aCAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAg                                                                                        >  3:194491/1‑141 (MQ=255)

                                                       cAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGt                                                                                       >  4:197320/1‑141 (MQ=255)

                                                         gTACTTTGTGAAGGAAGTAGTAAAAAAGATGATTAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTAt                                                                                     >  1:975373/1‑141 (MQ=255)

                                                         gTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTAt                                                                                     <  3:126207/141‑1 (MQ=255)

                                                         gTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTAt                                                                                     >  1:434067/1‑141 (MQ=255)

                                                           aCTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTc                                                                                   <  3:138336/141‑1 (MQ=255)

                                                           aCTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTc                                                                                   >  2:457632/1‑141 (MQ=255)

                                                             tttGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCAt                                                                                 >  2:464863/1‑141 (MQ=255)

                                                              ttGTGAAGGTAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCACCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCAtt                                                                                <  2:665213/141‑1 (MQ=255)

                                                                gtgAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTaa                                                                              >  2:138957/1‑141 (MQ=255)

                                                                   aaggaagTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATg                                                                           >  1:507687/1‑141 (MQ=255)

                                                                    aggaagTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATgg                                                                          <  2:769454/141‑1 (MQ=255)

                                                                     ggaagTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGc                                                                         >  1:84533/1‑141 (MQ=255)

                                                                     ggaagTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGc                                                                         >  4:146074/1‑141 (MQ=255)

                                                                      gtagtagtAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCACCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCa                                                                        >  1:291205/3‑141 (MQ=255)

                                                                      gaagTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCa                                                                        >  1:1077607/1‑141 (MQ=255)

                                                                        agtagtAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGt                                                                      >  2:965944/1‑141 (MQ=255)

                                                                          tagtaAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGttt                                                                    <  2:464694/141‑1 (MQ=255)

                                                                           agtaAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGtttt                                                                   >  2:779341/1‑141 (MQ=255)

                                                                            gtaAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTta                                                                  >  2:804174/1‑141 (MQ=255)

                                                                            gtaAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTta                                                                  <  3:9011/141‑1 (MQ=255)

                                                                             taAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTtat                                                                 <  1:682623/141‑1 (MQ=255)

                                                                             taAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTtat                                                                 >  2:353031/1‑141 (MQ=255)

                                                                                aaaaGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATaaa                                                              <  1:244927/141‑1 (MQ=255)

                                                                                   aGATTATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGa                                                           <  1:761694/141‑1 (MQ=255)

                                                                                   aGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGa                                                           <  2:600727/141‑1 (MQ=255)

                                                                                    gatgatCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAACCTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTAGAAAGGaa                                                          <  3:271776/141‑1 (MQ=255)

                                                                                     atgatCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAg                                                         <  1:360342/141‑1 (MQ=255)

                                                                                      tgatCAGGTTCTTGCTGGCTACACTGATAAACATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTa                                                                               >  1:845909/1‑118 (MQ=255)

                                                                                      tgatCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTa                                                                               <  2:845909/118‑1 (MQ=255)

                                                                                         tCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTaga                                                     >  2:762771/1‑141 (MQ=255)

                                                                                         tCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGCaga                                                     >  4:273133/1‑141 (MQ=255)

                                                                                          cAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCACCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTGTAAAGGAAGTagag                                                    <  1:79350/141‑1 (MQ=255)

                                                                                           aGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGc                                                   <  4:320607/141‑1 (MQ=255)

                                                                                             gTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCg                                                 <  1:714684/141‑1 (MQ=255)

                                                                                             gTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCg                                                 <  2:510407/141‑1 (MQ=255)

                                                                                             gTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCg                                                 >  1:412653/1‑141 (MQ=255)

                                                                                                cTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTATTGGAAGTACGAAGAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCTGaa                                              <  4:5329/141‑1 (MQ=255)

                                                                                                cTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGaa                                              <  3:159757/141‑1 (MQ=255)

                                                                                                cTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCACCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTGTAAAGGAAGTAGAGCCGGaa                                              <  1:370962/141‑1 (MQ=255)

                                                                                                    cTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATgg                                          >  1:49060/1‑141 (MQ=255)

                                                                                                      ggatACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTg                                        <  2:956104/138‑1 (MQ=255)

                                                                                                      ggCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTg                                        >  2:154607/1‑141 (MQ=255)

                                                                                                      ggCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTg                                        >  2:496520/1‑141 (MQ=255)

                                                                                                        cTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGAt                                      <  2:400854/141‑1 (MQ=255)

                                                                                                          acacTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTc                                    >  2:407773/1‑141 (MQ=255)

                                                                                                                aTAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATaaa                              >  4:192534/1‑141 (MQ=255)

                                                                                                                  aaaaaTAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATg                            <  4:215246/141‑1 (MQ=255)

                                                                                                                       tAAGCTTGTTAATTGCAAAGCGCCTAACGAAATGATTGGTAAACTAGTGGAAGTACAAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTataa                       <  1:309551/141‑1 (MQ=255)

                                                                                                                       tAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTataa                       >  2:544610/1‑141 (MQ=255)

                                                                                                                         aGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTataata                     >  1:181428/1‑141 (MQ=255)

                                                                                                                         aGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTataata                     >  1:767455/1‑141 (MQ=255)

                                                                                                                            tAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAg                  <  4:92945/141‑1 (MQ=255)

                                                                                                                             aGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGa                 >  1:620435/1‑141 (MQ=255)

                                                                                                                             aGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGa                 >  1:14245/1‑141 (MQ=255)

                                                                                                                                tAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGa              <  1:936790/141‑1 (MQ=255)

                                                                                                                                     tCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACgtgt         <  2:498977/141‑1 (MQ=255)

                                                                                                                                      cAAAGCGACTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGTAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGtt        <  2:998486/141‑1 (MQ=255)

                                                                                                                                        aaGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGa      >  2:1039715/1‑141 (MQ=255)

                                                                                                                                         aGCGCCTAAAGAAATGATTGGTAGACAAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGaa     <  4:87629/141‑1 (MQ=255)

                                                                                                                                            gcCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCa  >  3:222490/1‑141 (MQ=255)

                                                                                                                                            gcCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCa  >  2:363625/1‑141 (MQ=255)

AAGTTGGTCATTATTCAC‑AAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTGAAGGAAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCGCCTAAAGAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTATAAAGGAAGTAGAGCCGGAAATGGTGATTCAATAAATGTATAATAAAGATGACGTGTTGAACCA  >  USA300TCH1516_ALE/1319161‑1319440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`