Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,338,620 G→A 6.5% intergenic (+555/‑720) glnA → / → USA300TCH1516_01240 Glutamine synthetase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,338,6200GA 6.5% 276.0 / 5.6 91intergenic (+555/‑720)glnA/USA300TCH1516_01240Glutamine synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (39/46);  new base A (2/4);  total (41/50)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

TTATCGATCGGTATTTTCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATA  >  USA300TCH1516_ALE/1338481‑1338759
                                                                                                                                           |                                                                                                                                           
ttATCGATCGGTATTTTCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGa                                                                                                                                            <  2:488289/141‑1 (MQ=255)
 tATCGATCGGTATTTTCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAg                                                                                                                                           <  4:37000/141‑1 (MQ=255)
    cgatcgGTAGTTTCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCAACTAATTTATGAGTAGCGAGGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTa                                                                                                                                        >  1:1083484/1‑141 (MQ=255)
       tcgGTATTTTCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCa                                                                                                                                     <  2:404752/141‑1 (MQ=255)
       tcgGTATTTTCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCa                                                                                                                                     <  1:247010/141‑1 (MQ=255)
        cgGTATTTTCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCaa                                                                                                                                    <  1:1005113/141‑1 (MQ=255)
        cgGTATTTTCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCaa                                                                                                                                    >  2:396613/1‑141 (MQ=255)
        cgGTATTTTCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCaa                                                                                                                                    >  4:159008/1‑141 (MQ=255)
          gTATTTTCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAAt                                                                                                                                  <  2:391491/141‑1 (MQ=255)
          gTATTTTCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAAt                                                                                                                                  <  4:70365/141‑1 (MQ=255)
            aTTTTCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATta                                                                                                                                >  1:746696/1‑141 (MQ=255)
              tttCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATtaat                                                                                                                              <  1:133124/141‑1 (MQ=255)
               ttCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATtaata                                                                                                                             <  1:170819/141‑1 (MQ=255)
                tCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTGCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATtaataa                                                                                                                            <  1:284767/141‑1 (MQ=255)
                  aTTTTAGTTGTACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATaaaa                                                                                                                          >  1:956501/1‑140 (MQ=255)
                     ttAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATa                                                                                                                       <  2:663633/141‑1 (MQ=255)
                     ttAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATCAATAAAGATa                                                                                                                       <  3:277605/141‑1 (MQ=255)
                        gTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTGATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGCTAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTg                                                                                                                    >  4:166782/1‑141 (MQ=255)
                        gTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTg                                                                                                                    >  4:222945/1‑141 (MQ=255)
                        gTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTg                                                                                                                    <  2:874826/141‑1 (MQ=255)
                        gTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTg                                                                                                                    >  4:152701/1‑141 (MQ=255)
                          tataCTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTggg                                                                                                                  <  4:351362/141‑1 (MQ=255)
                           ataCTACTATTGATATTCTAAAAAATTAAACATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGa                                                                                                                 <  2:774482/141‑1 (MQ=255)
                             actactATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATa                                                                                                               <  2:832895/141‑1 (MQ=255)
                                  taTTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTa                                                                                                          >  3:219499/1‑141 (MQ=255)
                                     tGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACgg                                                                                                       <  1:517973/141‑1 (MQ=255)
                                        tatTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTaa                                                                                                    >  1:260669/1‑141 (MQ=255)
                                        tatTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTaa                                                                                                    >  1:418670/1‑141 (MQ=255)
                                            cTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATAtt                                                                                                >  1:752510/1‑141 (MQ=255)
                                             tAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTa                                                                                               >  2:1024088/1‑141 (MQ=255)
                                                aaaattaaaaattTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAAAATTGATAGAAAACAAAGAGATATCAAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATg                                                                                            >  2:568604/1‑141 (MQ=255)
                                                 aaattaaaaattTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGc                                                                                           <  2:460921/141‑1 (MQ=255)
                                                    ttaaaaattTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTAt                                                                                        >  3:424203/1‑141 (MQ=255)
                                                            ttCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTa                                                                                <  1:423430/141‑1 (MQ=255)
                                                                 cTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGagaa                                                                           <  2:397479/141‑1 (MQ=255)
                                                                 cTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGagaa                                                                           >  2:980116/1‑141 (MQ=255)
                                                                 cTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGagaa                                                                           >  2:612078/1‑141 (MQ=255)
                                                                    aTCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAAAATTGATAGAAAACAAAGAGATATCAAGTTATCAAATTAATAAATATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTGGggaaaga                                                                        >  2:547392/1‑141 (MQ=255)
                                                                       tAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAAAATTGATAGAAAACAAAGAGATATCAAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTGGGGAAagaaga                                                                     <  1:625598/141‑1 (MQ=255)
                                                                        aaTTTAGAAATTGCTATGACGAAATATTCAATACAATCGAAGAATTGATAGAAAAAAAAGATAGAGCGAGTTATCAAATTAATAAAGATACTGGTATAAGTTACGTTAATATTAATGCTATGAGCCGTAGAGAAAtaagaa                                                                    <  4:122516/141‑1 (MQ=255)
                                                                         aTTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATAGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGGAAGAAGAAt                                                                   >  4:255096/1‑141 (MQ=255)
                                                                           ttATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAg                                                                 <  3:300001/141‑1 (MQ=255)
                                                                           ttATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAg                                                                 <  1:879798/141‑1 (MQ=255)
                                                                            tATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGAGACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGa                                                                <  2:1065514/141‑1 (MQ=255)
                                                                              tGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGaaa                                                              >  2:882145/1‑141 (MQ=255)
                                                                                 aTTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAAtt                                                           <  4:405296/141‑1 (MQ=255)
                                                                                  ttGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAAttt                                                          >  3:305664/1‑141 (MQ=255)
                                                                                  ttGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAAttt                                                          >  3:305818/1‑141 (MQ=255)
                                                                                   tGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTa                                                         >  2:428965/1‑141 (MQ=255)
                                                                                      tATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGc                                                      <  2:580040/141‑1 (MQ=255)
                                                                                      tATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGc                                                      <  1:564536/141‑1 (MQ=255)
                                                                                      tATGATGAAATATTCAATACAATCAAAAAATTGATAGAAAACAAAGAGATATCAAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTGGGGAAAGAAGAATAGAAAATTTAAGc                                                      <  1:492352/141‑1 (MQ=255)
                                                                                        tgatgaAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCtt                                                    <  2:18072/141‑1 (MQ=255)
                                                                                         gatgaAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTa                                                   >  4:78585/1‑141 (MQ=255)
                                                                                          atgaAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTaa                                                  <  3:285/141‑1 (MQ=255)
                                                                                          atgaAATATTCAATAAAATCAAAAAATGGATAGAAAACCAAGAGATATCAAGTTATCAAATTAATAAATATACTGGGATAAGTTACGGTAATATTAATGCTATGCGACGTGGGGAAAGAAGAATAGAAAATTTAAGCTTaa                                                  <  4:327182/141‑1 (MQ=255)
                                                                                           tgaAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTaaa                                                 <  4:396997/141‑1 (MQ=255)
                                                                                               atatTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAAt                                             >  3:430537/1‑141 (MQ=255)
                                                                                               atatTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATCTAAGCTTAAAGAAt                                             >  1:13461/1‑141 (MQ=255)
                                                                                                 atTCAATACAATCAACGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTCACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATGTAAGCTTAAAGAATGc                                           >  2:866538/1‑141 (MQ=255)
                                                                                                 atTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGc                                           >  2:903549/1‑141 (MQ=255)
                                                                                                 atTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGc                                           <  2:965820/141‑1 (MQ=255)
                                                                                                   tCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCaa                                         <  4:305818/141‑1 (MQ=255)
                                                                                                   tCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCaa                                         <  4:305664/141‑1 (MQ=255)
                                                                                                   tCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCaa                                         >  2:696708/1‑141 (MQ=255)
                                                                                                    cAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCaaa                                        <  4:219499/141‑1 (MQ=255)
                                                                                                    cAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCaaa                                        >  2:476401/1‑141 (MQ=255)
                                                                                                       tACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGAt                                     <  1:1021219/141‑1 (MQ=255)
                                                                                                       tACAATCAAAAAATTGATAGAAAACAAAGAGATATCAAGTTATCAAATTAATAAATATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTGGGGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGAt                                     <  1:547392/141‑1 (MQ=255)
                                                                                                            tCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTtat                                <  3:221721/141‑1 (MQ=255)
                                                                                                            tCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTtat                                <  3:152597/141‑1 (MQ=255)
                                                                                                              aaaGAATTGATAGAAAACAAAGAGATATCGTGTTATCAATTTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTatat                              >  2:381837/1‑141 (MQ=255)
                                                                                                               attctttgattgaAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGTGATAAGTTACGGTAATATTAATGCTATACGCCGAAGAGAAAAAAGAATAGAAAATTTAAACTTAAAGAATGAAAAGATCTtataga                             >  4:419945/6‑139 (MQ=255)
                                                                                                                   aTTGATAGAAAACAAGGCGATATCGAGTTCTCAAGTTAATAAAGATACTGGGATAAGTTACGGTAATATCAAGGCTCTGCGCCGTAGAGAAAGAAGAACAGAAAACGTAAGCGTAAAGAATGCAAACATCTTATATGAata                         <  2:512548/141‑1 (MQ=255)
                                                                                                                   aTTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAata                         <  2:595754/141‑1 (MQ=255)
                                                                                                                   aTTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAata                         >  2:741532/1‑141 (MQ=255)
                                                                                                                     tGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACCGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAGAGATCTTATATGAATATg                       >  2:246894/1‑141 (MQ=255)
                                                                                                                         aGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGaa                   <  2:821050/141‑1 (MQ=255)
                                                                                                                           aaaaCAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGaaaa                 >  2:1070622/1‑141 (MQ=255)
                                                                                                                              aCAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAgg              <  1:1024088/141‑1 (MQ=255)
                                                                                                                              aCAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAgg              >  1:1047263/1‑141 (MQ=255)
                                                                                                                               cAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATTCAAAGATCTTATAAGAAAAAGAGAAAAAGGt             <  2:917622/141‑1 (MQ=255)
                                                                                                                                  agagATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTAtt          >  2:784967/1‑141 (MQ=255)
                                                                                                                                  agagATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTAtt          >  1:1056752/1‑141 (MQ=255)
                                                                                                                                  agagATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTAAGGTAATATTAAAGCTATGAGACATAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTAtt          <  1:213035/141‑1 (MQ=255)
                                                                                                                                    agaTATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGt        >  4:51260/1‑141 (MQ=255)
                                                                                                                                    agaTATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGAGACAAAGGAATTGt        >  3:170707/1‑141 (MQ=255)
                                                                                                                                      atatCGAGTTATCAAAATAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTAAATGAAAAAAAAAAAAAGGTATTGTaa      <  2:331375/141‑1 (MQ=255)
                                                                                                                                         tCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAatat   <  4:277120/141‑1 (MQ=255)
                                                                                                                                         tCGAGTTATCAAATTAATAAAGATACTGGGATAAGATACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAatat   <  2:84622/141‑1 (MQ=255)
                                                                                                                                          cGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAAtata  >  1:951098/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TTATCGATCGGTATTTTCATTTTAGTTATACTACTATTGATATTCTAAAAAATTAAAAATTTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATA  >  USA300TCH1516_ALE/1338481‑1338759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: