Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,338,681 A→G 6.6% intergenic (+616/‑659) glnA → / → USA300TCH1516_01240 Glutamine synthetase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,338,6810AG 6.6% 268.4 / 6.8 91intergenic (+616/‑659)glnA/USA300TCH1516_01240Glutamine synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (38/47);  new base G (2/4);  total (40/51)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

TTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGCCCAAACTAGTGACTGAGTGGGTGCTTT  >  USA300TCH1516_ALE/1338541‑1338818
                                                                                                                                            |                                                                                                                                         
ttCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTa                                                                                                                                           <  1:423430/141‑1 (MQ=255)
     cTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGagaa                                                                                                                                      >  2:612078/1‑141 (MQ=255)
     cTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGagaa                                                                                                                                      <  2:397479/141‑1 (MQ=255)
     cTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGagaa                                                                                                                                      >  2:980116/1‑141 (MQ=255)
        aTCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAAAATTGATAGAAAACAAAGAGATATCAAGTTATCAAATTAATAAATATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTGGggaaaga                                                                                                                                   >  2:547392/1‑141 (MQ=255)
           tAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAAAATTGATAGAAAACAAAGAGATATCAAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTGGGGAAagaaga                                                                                                                                <  1:625598/141‑1 (MQ=255)
            aaTTTAGAAATTGCTATGACGAAATATTCAATACAATCGAAGAATTGATAGAAAAAAAAGATAGAGCGAGTTATCAAATTAATAAAGATACTGGTATAAGTTACGTTAATATTAATGCTATGAGCCGTAGAGAAAtaagaa                                                                                                                               <  4:122516/141‑1 (MQ=255)
             aTTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATAGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGGAAGAAGAAt                                                                                                                              >  4:255096/1‑141 (MQ=255)
               ttATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAg                                                                                                                            <  1:879798/141‑1 (MQ=255)
               ttATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAg                                                                                                                            <  3:300001/141‑1 (MQ=255)
                tATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGAGACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGa                                                                                                                           <  2:1065514/141‑1 (MQ=255)
                  tGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGaaa                                                                                                                         >  2:882145/1‑141 (MQ=255)
                     aTTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAAtt                                                                                                                      <  4:405296/141‑1 (MQ=255)
                      ttGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAAttt                                                                                                                     >  3:305664/1‑141 (MQ=255)
                      ttGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAAttt                                                                                                                     >  3:305818/1‑141 (MQ=255)
                       tGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTa                                                                                                                    >  2:428965/1‑141 (MQ=255)
                          tATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGc                                                                                                                 <  2:580040/141‑1 (MQ=255)
                          tATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGc                                                                                                                 <  1:564536/141‑1 (MQ=255)
                          tATGATGAAATATTCAATACAATCAAAAAATTGATAGAAAACAAAGAGATATCAAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTGGGGAAAGAAGAATAGAAAATTTAAGc                                                                                                                 <  1:492352/141‑1 (MQ=255)
                            tgatgaAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCtt                                                                                                               <  2:18072/141‑1 (MQ=255)
                             gatgaAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTa                                                                                                              >  4:78585/1‑141 (MQ=255)
                              atgaAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTaa                                                                                                             <  3:285/141‑1 (MQ=255)
                              atgaAATATTCAATAAAATCAAAAAATGGATAGAAAACCAAGAGATATCAAGTTATCAAATTAATAAATATACTGGGATAAGTTACGGTAATATTAATGCTATGCGACGTGGGGAAAGAAGAATAGAAAATTTAAGCTTaa                                                                                                             <  4:327182/141‑1 (MQ=255)
                               tgaAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTaaa                                                                                                            <  4:396997/141‑1 (MQ=255)
                                   atatTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAAt                                                                                                        >  3:430537/1‑141 (MQ=255)
                                   atatTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATCTAAGCTTAAAGAAt                                                                                                        >  1:13461/1‑141 (MQ=255)
                                     atTCAATACAATCAACGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTCACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATGTAAGCTTAAAGAATGc                                                                                                      >  2:866538/1‑141 (MQ=255)
                                     atTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGc                                                                                                      <  2:965820/141‑1 (MQ=255)
                                     atTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGc                                                                                                      >  2:903549/1‑141 (MQ=255)
                                       tCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCaa                                                                                                    >  2:696708/1‑141 (MQ=255)
                                       tCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCaa                                                                                                    <  4:305818/141‑1 (MQ=255)
                                       tCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCaa                                                                                                    <  4:305664/141‑1 (MQ=255)
                                        cAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCaaa                                                                                                   >  2:476401/1‑141 (MQ=255)
                                        cAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCaaa                                                                                                   <  4:219499/141‑1 (MQ=255)
                                           tACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGAt                                                                                                <  1:1021219/141‑1 (MQ=255)
                                           tACAATCAAAAAATTGATAGAAAACAAAGAGATATCAAGTTATCAAATTAATAAATATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTGGGGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGAt                                                                                                <  1:547392/141‑1 (MQ=255)
                                                tCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTtat                                                                                           <  3:152597/141‑1 (MQ=255)
                                                tCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTtat                                                                                           <  3:221721/141‑1 (MQ=255)
                                                  aaaGAATTGATAGAAAACAAAGAGATATCGTGTTATCAATTTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTatat                                                                                         >  2:381837/1‑141 (MQ=255)
                                                   attctttgattgaAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGTGATAAGTTACGGTAATATTAATGCTATACGCCGAAGAGAAAAAAGAATAGAAAATTTAAACTTAAAGAATGAAAAGATCTtataga                                                                                        >  4:419945/6‑139 (MQ=255)
                                                       aTTGATAGAAAACAAGGCGATATCGAGTTCTCAAGTTAATAAAGATACTGGGATAAGTTACGGTAATATCAAGGCTCTGCGCCGTAGAGAAAGAAGAACAGAAAACGTAAGCGTAAAGAATGCAAACATCTTATATGAata                                                                                    <  2:512548/141‑1 (MQ=255)
                                                       aTTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAata                                                                                    >  2:741532/1‑141 (MQ=255)
                                                       aTTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAata                                                                                    <  2:595754/141‑1 (MQ=255)
                                                         tGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACCGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAGAGATCTTATATGAATATg                                                                                  >  2:246894/1‑141 (MQ=255)
                                                             aGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGaa                                                                              <  2:821050/141‑1 (MQ=255)
                                                               aaaaCAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGaaaa                                                                            >  2:1070622/1‑141 (MQ=255)
                                                                  aCAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAgg                                                                         <  1:1024088/141‑1 (MQ=255)
                                                                  aCAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAgg                                                                         >  1:1047263/1‑141 (MQ=255)
                                                                   cAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATTCAAAGATCTTATAAGAAAAAGAGAAAAAGGt                                                                        <  2:917622/141‑1 (MQ=255)
                                                                      agagATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTAtt                                                                     >  2:784967/1‑141 (MQ=255)
                                                                      agagATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTAtt                                                                     >  1:1056752/1‑141 (MQ=255)
                                                                      agagATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTAAGGTAATATTAAAGCTATGAGACATAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTAtt                                                                     <  1:213035/141‑1 (MQ=255)
                                                                        agaTATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGt                                                                   >  4:51260/1‑141 (MQ=255)
                                                                        agaTATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGAGACAAAGGAATTGt                                                                   >  3:170707/1‑141 (MQ=255)
                                                                          atatCGAGTTATCAAAATAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTAAATGAAAAAAAAAAAAAGGTATTGTaa                                                                 <  2:331375/141‑1 (MQ=255)
                                                                             tCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAatat                                                              <  4:277120/141‑1 (MQ=255)
                                                                             tCGAGTTATCAAATTAATAAAGATACTGGGATAAGATACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAatat                                                              <  2:84622/141‑1 (MQ=255)
                                                                              cGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAAtata                                                             >  1:951098/1‑141 (MQ=255)
                                                                                   tATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCAt                                                        <  3:374990/141‑1 (MQ=255)
                                                                                   aaTCAAAGTAATAAGTATACAGAGAAAAGTTACGGTAAAATTAATGCTATGCGCCGTATAGAAAAAAGAATAGAAAATTTAAGCTTAAAGAATGCACAGATCTTATATAAAAAAAAAAAAAAGGTATTGTAATATAAGCAt                                                        <  2:692445/140‑1 (MQ=255)
                                                                                     tCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTg                                                      >  4:29429/1‑141 (MQ=255)
                                                                                     tCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTg                                                      >  1:240831/1‑141 (MQ=255)
                                                                                     tCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTg                                                      <  1:199368/141‑1 (MQ=255)
                                                                                      cAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGa                                                     >  1:700307/1‑141 (MQ=255)
                                                                                       aaaTTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAg                                                    >  1:335586/1‑141 (MQ=255)
                                                                                        aaTTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAATAATAGTTAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGt                                                   >  3:201364/1‑141 (MQ=255)
                                                                                        aaTTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGt                                                   <  3:74208/141‑1 (MQ=255)
                                                                                         aTTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGtt                                                  >  4:81926/1‑141 (MQ=255)
                                                                                         aTTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGtt                                                  >  1:753085/1‑141 (MQ=255)
                                                                                         aTTAATAAAGATACTGGGATAAGTTAAGGTAATATAAATGGTATGCGCAGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTAAAAAAAAAAAAAAAAAAGGTATTGTAATATAAGCATTGAGtt                                                  <  2:338117/141‑1 (MQ=255)
                                                                                           taataaAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCa                                                <  1:889195/141‑1 (MQ=255)
                                                                                             ataaAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAAt                                              >  2:241841/1‑141 (MQ=255)
                                                                                              taaAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATAAAAAAAAAAAAAAAAGGTATTGTAATATAAGCATTGAGTTCAATa                                             <  2:59878/141‑1 (MQ=255)
                                                                                                 aGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGc                                          <  1:726701/141‑1 (MQ=255)
                                                                                                   aTACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCAt                                        <  2:802645/141‑1 (MQ=255)
                                                                                                    tACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATa                                       <  1:711632/141‑1 (MQ=255)
                                                                                                      cTGGGATAAGTTACGGNAATATTAATGCTNTGCGCCGTGGGGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACa                                     >  1:865535/1‑141 (MQ=255)
                                                                                                             aaGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTg                              <  4:196213/141‑1 (MQ=255)
                                                                                                               gTTACGGTAATTTTATTGCTACGCGCCGTAGAGAAAGAAAAATAGAAAATATAAGCTTAAAGAATGCAAAGATCTTTTATGAATTAGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGcc                            <  2:889572/141‑1 (MQ=255)
                                                                                                                 tACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGCCCa                          >  2:683207/1‑141 (MQ=255)
                                                                                                                 tACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGCCCa                          <  1:714146/141‑1 (MQ=255)
                                                                                                                 tACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGCCCa                          <  2:1005113/141‑1 (MQ=255)
                                                                                                                  aCGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGCCCaa                         >  1:479309/1‑141 (MQ=255)
                                                                                                                           ttAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTatat                                                                                         >  2:873527/1‑68 (MQ=255)
                                                                                                                           ttAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTatat                                                                                         <  1:873527/68‑1 (MQ=255)
                                                                                                                             aaTGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGCCCAAACTAGTGACTg              >  1:891404/1‑141 (MQ=255)
                                                                                                                               tGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGCCCAAACTAGTGACTGAg            <  1:412513/141‑1 (MQ=255)
                                                                                                                               tGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGAGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGCCCAAACTAGTGACTGAg            <  2:458776/141‑1 (MQ=255)
                                                                                                                                   aTGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGCCCAAACTAGTGACTGAGTggg        <  3:124803/141‑1 (MQ=255)
                                                                                                                                      cgcCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGCCCAAACTAGTGACTGAGTGGGTGc     <  3:186823/141‑1 (MQ=255)
                                                                                                                                        ccGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGCCCAAACTAGTGACTGAGTGGGTGCtt   <  3:152701/141‑1 (MQ=255)
                                                                                                                                         cGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGCCCAAACTAGTGACTGAGTGGGTGCttt  >  2:56487/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TTCGCCTCATCTAATTTATGAATTGCTATGATGAAATATTCAATACAATCAAAGAATTGATAGAAAACAAAGAGATATCGAGTTATCAAATTAATAAAGATACTGGGATAAGTTACGGTAATATTAATGCTATGCGCCGTAGAGAAAGAAGAATAGAAAATTTAAGCTTAAAGAATGCAAAGATCTTATATGAATATGCGAAAAAGGTATTGTAATATAAGCATTGAGTTCAATAGGCATACAATAACTGCCCAAACTAGTGACTGAGTGGGTGCTTT  >  USA300TCH1516_ALE/1338541‑1338818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: