BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,341,936	C→A	5.7%	intergenic (+344/‑168)	USA300HOU_RS06615 → / → USA300TCH1516_01244	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,341,936	0	C	A	5.7%	365.0 / 7.4	114	intergenic (+344/‑168)	USA300HOU_RS06615/USA300TCH1516_01244	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (50/57); new base A (4/3); total (54/60)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

Only 100 of 116 total aligned reads displayed.

AAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATACTTGCT  >  USA300TCH1516_ALE/1341798‑1342076

aaaaGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGa                                                                                                                                            <  2:622039/141‑1 (MQ=255)

 aaaGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAg                                                                                                                                           <  1:893794/141‑1 (MQ=255)

   aGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTg                                                                                                                                         >  1:902788/1‑141 (MQ=255)

   aGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTg                                                                                                                                         <  3:99036/141‑1 (MQ=255)

   aGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTg                                                                                                                                         <  1:238312/141‑1 (MQ=255)

    gATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGa                                                                                                                                        >  3:53035/1‑141 (MQ=255)

        ggAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGa                                                                                                                                    <  3:377929/141‑1 (MQ=255)

        ggAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGa                                                                                                                                    <  1:244204/141‑1 (MQ=255)

        ggAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGa                                                                                                                                    >  1:288414/1‑141 (MQ=255)

              gATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATaaa                                                                                                                              >  2:427820/1‑141 (MQ=255)

                tCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTAAAAGTCGAATTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAaaaaaaaaaaaa                                                                                                                            <  4:208160/141‑1 (MQ=39)

                   tCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTTTTTTTTTTTTTTTTTTTGTTCTTTTTCTTTTTTTTTTTTTATCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGTAATAAAAAAGc                                                                                                                         >  4:115792/1‑141 (MQ=255)

                   tCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGc                                                                                                                         >  3:182271/1‑141 (MQ=255)

                        aaaTGCTCCCTTCAAAGTTTTCATTTTTGCAATGTCGACGTCGAAGGGGGCGTTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGGGAAGGAAATAAAAAAGCTCaaa                                                                                                                    >  4:329570/1‑141 (MQ=255)

                          aTGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATg                                                                                                                  <  1:444294/141‑1 (MQ=255)

                          aTGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATg                                                                                                                  <  2:1065953/141‑1 (MQ=255)

                            gCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTa                                                                                                                <  3:205155/141‑1 (MQ=255)

                            gCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTa                                                                                                                >  1:87425/1‑141 (MQ=255)

                               cccTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCa                                                                                                             >  3:421918/1‑141 (MQ=255)

                                 cTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCaaa                                                                                                           <  2:424719/141‑1 (MQ=255)

                                      aaGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGtt                                                                                                      <  1:330067/141‑1 (MQ=255)

                                         ttttCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGCTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCCCAAATGTACCAAATTGTTAAt                                                                                                   >  1:1060926/1‑141 (MQ=255)

                                           ttCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCt                                                                                                 >  1:1056057/1‑141 (MQ=255)

                                           ttCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCt                                                                                                 >  1:1003280/1‑141 (MQ=255)

                                              aTTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTtaa                                                                                              >  1:630735/1‑141 (MQ=255)

                                               ttttttCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTtaat                                                                                             >  2:875874/1‑141 (MQ=255)

                                                tttttCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTtaata                                                                                            <  1:538264/141‑1 (MQ=255)

                                                tttttCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATCGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTtaata                                                                                            >  2:934058/1‑141 (MQ=255)

                                                   ttttttGTCGACAACGCAGGCGGCATTTTCATTAAATTGTTATAGCTTTTTATCTTTGTATAATGAACATATTATTTTTATTTTTTTCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAAt                                                                                         >  2:1080651/6‑141 (MQ=255)

                                                   ttCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAAt                                                                                         <  2:566413/141‑1 (MQ=255)

                                                    tCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAAtc                                                                                        >  2:1084416/1‑141 (MQ=255)

                                                        tGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTa                                                                                    >  1:658064/1‑141 (MQ=255)

                                                         gtcgtcTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTAc                                                                                   <  2:753710/141‑1 (MQ=255)

                                                         gTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTAc                                                                                   <  3:136970/141‑1 (MQ=255)

                                                         gTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTAc                                                                                   <  1:207381/141‑1 (MQ=255)

                                                          tCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACt                                                                                  >  4:454277/1‑141 (MQ=255)

                                                            gtgttCGAAGGGGGCATTTTCATTAAATTGTTGTAGGTGTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGTAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACttt                                                                                <  2:942859/138‑1 (MQ=255)

                                                              cTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTtat                                                                              >  1:352606/1‑141 (MQ=255)

                                                               ttCGAAGGGGGCGTTTTCATTAAATTGTTATAGCTTTTTATATTTGTATGATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTtata                                                                             <  2:649145/141‑1 (MQ=255)

                                                               ttCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTtata                                                                             >  1:185597/1‑141 (MQ=255)

                                                               ttCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTtata                                                                             <  1:328185/141‑1 (MQ=255)

                                                                tCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATaa                                                                            <  2:236199/141‑1 (MQ=255)

                                                                tCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATaa                                                                            <  2:147156/141‑1 (MQ=255)

                                                                    aGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGAt                                                                        <  1:454950/141‑1 (MQ=255)

                                                                     gggggCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGAtt                                                                       >  1:872028/1‑141 (MQ=255)

                                                                      ggggCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTg                                                                      >  1:1065926/1‑141 (MQ=255)

                                                                      ggggCATTTCCATTATATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTg                                                                      >  2:985215/1‑141 (MQ=255)

                                                                          cATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATg                                                                  <  4:403850/141‑1 (MQ=255)

                                                                             tttCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGAc                                                               >  2:212326/1‑141 (MQ=255)

                                                                              ttCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACa                                                              <  1:81477/141‑1 (MQ=255)

                                                                                  ttataTTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCg                                                          >  1:85716/5‑141 (MQ=255)

                                                                                  ttAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCg                                                          <  1:646146/141‑1 (MQ=255)

                                                                                   tAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGAGAAAGAAAAAAAAAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGa                                                         <  2:134415/141‑1 (MQ=255)

                                                                                    ataTTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAg                                                        <  2:483140/139‑1 (MQ=255)

                                                                                       ttGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGt                                                     >  3:275210/1‑141 (MQ=255)

                                                                                         gTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTa                                                   >  4:105970/1‑141 (MQ=255)

                                                                                          ttATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTaa                                                  >  1:916562/1‑141 (MQ=255)

                                                                                          ttATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTaa                                                  >  2:625314/1‑141 (MQ=255)

                                                                                           tataGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAt                                                 >  4:297739/1‑141 (MQ=255)

                                                                                           tataGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAt                                                 <  3:424438/141‑1 (MQ=255)

                                                                                            ataGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtc                                                <  2:335370/141‑1 (MQ=255)

                                                                                             taGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtca                                               <  1:913020/141‑1 (MQ=255)

                                                                                              aGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAATCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtcag                                              >  3:354052/1‑141 (MQ=255)

                                                                                              aGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtcat                                              >  2:809040/1‑140 (MQ=255)

                                                                                               gCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtcagt                                             <  2:468720/141‑1 (MQ=255)

                                                                                                 ttttttTAGTTGTATAATGAACCTATAAGTTGAAGTAAGTGCTGATGTAAATAATAAAAAAGCTCAAATGTACCAAATGGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtcagtca                                           <  3:153132/141‑1 (MQ=255)

                                                                                                 tttttATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtcagtca                                           <  2:591297/141‑1 (MQ=255)

                                                                                                   tttATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                         <  1:20806/141‑1 (MQ=255)

                                                                                                   tttATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                         <  1:886013/141‑1 (MQ=255)

                                                                                                   tttATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                         <  2:352606/141‑1 (MQ=255)

                                                                                                   tttATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                         >  3:182714/1‑141 (MQ=255)

                                                                                                   tttATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                         >  2:285706/1‑141 (MQ=255)

                                                                                                      gtatTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAGTGGACATTCGAGCGTTAATCAGTCaggagg                                      <  2:491687/140‑1 (MQ=255)

                                                                                                      atatTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCaggagg                                      >  2:33906/1‑141 (MQ=255)

                                                                                                      atatTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCaggagg                                      <  2:70138/141‑1 (MQ=255)

                                                                                                         tttGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGAc                                   >  1:684973/1‑141 (MQ=255)

                                                                                                          ttGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGaaa                                  <  1:694239/141‑3 (MQ=255)

                                                                                                          ttGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACt                                  <  2:217378/141‑1 (MQ=255)

                                                                                                           tGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACtt                                 <  1:684333/141‑1 (MQ=255)

                                                                                                            gTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGaaagt                                <  3:238977/141‑5 (MQ=255)

                                                                                                            gTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACttt                                <  4:450547/141‑1 (MQ=255)

                                                                                                              ataATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGaaagtcc                              <  4:240895/141‑7 (MQ=255)

                                                                                                                 aTGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACAATCGAGCGTTAATCAGTTAGGAGGGACTTTCactc                           >  2:403135/1‑141 (MQ=255)

                                                                                                                  tGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCcctcc                          >  3:105333/1‑141 (MQ=255)

                                                                                                                      cATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACa                      >  2:36375/1‑141 (MQ=255)

                                                                                                                           aaGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTta                 >  2:890315/1‑141 (MQ=255)

                                                                                                                            aGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaa                >  1:271063/1‑141 (MQ=255)

                                                                                                                              tttAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaata              >  2:512776/1‑141 (MQ=255)

                                                                                                                                tAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaataat            <  3:424575/141‑1 (MQ=255)

                                                                                                                                tAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaataat            <  1:82241/141‑1 (MQ=255)

                                                                                                                                tAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaataat            <  2:630735/141‑1 (MQ=255)

                                                                                                                                   gaagGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTaataataat         >  1:320746/1‑141 (MQ=255)

                                                                                                                                    aagGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAataataata        >  1:737256/1‑141 (MQ=255)

                                                                                                                                     agGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTATTCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATAc       >  4:425258/1‑141 (MQ=255)

                                                                                                                                         gCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATACTTGc   >  1:886470/1‑141 (MQ=255)

                                                                                                                                         gCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATACTTGc   <  1:1070724/141‑1 (MQ=255)

                                                                                                                                         gCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATACTTGc   <  1:1070687/141‑1 (MQ=255)

                                                                                                                                         gCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATACTTGc   <  1:946549/141‑1 (MQ=255)

                                                                                                                                          cGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATACTTGCt  <  1:979694/141‑1 (MQ=255)

                                                                                                                                          cGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATACTTGCt  <  2:143842/141‑1 (MQ=255)

AAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATACTTGCT  >  USA300TCH1516_ALE/1341798‑1342076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`