Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,346,110 G→C 5.2% intergenic (+333/+216) USA300HOU_RS06685 → / ← ltaE hypothetical protein/Low specificity L‑threonine aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,346,1100GC 5.2% 361.0 / 8.1 112intergenic (+333/+216)USA300HOU_RS06685/ltaEhypothetical protein/Low specificity L‑threonine aldolase
Reads supporting (aligned to +/- strand):  ref base G (40/66);  new base C (2/4);  total (42/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 119 total aligned reads displayed.

CATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATA  >  USA300TCH1516_ALE/1345972‑1346250
                                                                                                                                          |                                                                                                                                            
cATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATgtc                                                                                                                                            <  2:686777/141‑1 (MQ=255)
 aTCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATgtcg                                                                                                                                           <  1:843123/141‑1 (MQ=255)
 aTCGCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATgtcg                                                                                                                                           <  4:434577/141‑1 (MQ=255)
  tctcCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATgtcgt                                                                                                                                          <  2:194921/141‑1 (MQ=255)
  gctcCATATTAAAAACTTAAATAGTGCATTCCATGTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATgtcgt                                                                                                                                          <  2:894006/140‑1 (MQ=255)
    tcCATATTAAAAACTTAAATAGTGCATTTCATTTAAACTAAACATAGATATTCTTACAAATATATACTTAGTAATTCGATTGTTAATACATCACTTTCATAAAATAACTATAACCATCATAATACTTCTCAAATCTCGTCa                                                                                                                                        <  1:98612/141‑1 (MQ=255)
      cATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCaaa                                                                                                                                      <  2:138366/141‑1 (MQ=255)
      cATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCaaa                                                                                                                                      >  1:321391/1‑141 (MQ=255)
      cATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCaaa                                                                                                                                      >  1:618811/1‑141 (MQ=255)
      cATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCaaa                                                                                                                                      >  1:618470/1‑141 (MQ=255)
       atatTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAAt                                                                                                                                     <  3:67859/141‑1 (MQ=255)
        tatTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAAtt                                                                                                                                    >  1:533758/1‑141 (MQ=255)
        tatTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAAtt                                                                                                                                    >  1:533612/1‑141 (MQ=255)
          ttAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTaa                                                                                                                                  >  4:176066/1‑141 (MQ=255)
              aaaCTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTa                                                                                                                              <  2:355721/141‑1 (MQ=255)
               aaCTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTaa                                                                                                                             >  4:304593/1‑141 (MQ=255)
                  ttAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTCCCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAATATTTCTTCATCCATTATATTACTTCTCAAATGTCGGCAAATTAAAGTAAATa                                                                                                                          <  1:285437/141‑1 (MQ=255)
                   tAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATAc                                                                                                                         >  1:88080/1‑141 (MQ=255)
                      aTAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGc                                                                                                                      <  2:113192/141‑1 (MQ=255)
                       tAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGca                                                                                                                     <  2:1082230/141‑1 (MQ=255)
                            cATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAAt                                                                                                                <  1:403475/141‑1 (MQ=255)
                              ttCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTa                                                                                                              <  3:405371/141‑1 (MQ=255)
                                ccATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAAc                                                                                                            <  4:410247/141‑1 (MQ=255)
                                ccATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAAc                                                                                                            <  1:671758/141‑1 (MQ=255)
                                ccATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAAc                                                                                                            <  1:6605/141‑1 (MQ=255)
                                   tttAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCGCAAATGTCTTCAAATTAAAGTAAATACAGCACAAATTAACAtt                                                                                                         <  1:414640/141‑1 (MQ=255)
                                     taaactaaacATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACAtttt                                                                                                       >  1:927043/1‑141 (MQ=255)
                                     taaactaaacATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACAtttt                                                                                                       >  2:639927/1‑141 (MQ=255)
                                        actaaacATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATCATCCTTCTAACCTGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTaa                                                                                                    >  3:41042/1‑141 (MQ=255)
                                        actaaacATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTaa                                                                                                    >  1:265815/1‑141 (MQ=255)
                                        actaaacATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTAAAACAATCATCATCATTCCCAAATGTCGCCAAATTAAAGTAAATACAGCACAAATTAACATTTTTaa                                                                                                    >  3:41019/1‑141 (MQ=255)
                                               aTCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAAtatata                                                                                             >  2:320232/1‑141 (MQ=255)
                                                tCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataa                                                                                            <  2:85197/141‑1 (MQ=255)
                                                 cGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaa                                                                                           <  1:931818/141‑1 (MQ=255)
                                                 cGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaa                                                                                           <  4:12739/141‑1 (MQ=255)
                                                 cGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTAAAATTAATGTAACTCTAACACAAATTAACATTTTTAAATATataaa                                                                                           <  2:48479/141‑1 (MQ=255)
                                                 cGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACAACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaa                                                                                           >  3:400922/1‑141 (MQ=255)
                                                    tatTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaaata                                                                                        >  2:401372/1‑141 (MQ=255)
                                                    tagtCCGACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaaata                                                                                        >  2:482505/4‑141 (MQ=255)
                                                     atTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaaataa                                                                                       <  1:54466/141‑1 (MQ=255)
                                                     atTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACCTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATataaaataa                                                                                       <  1:54695/141‑1 (MQ=255)
                                                       tCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATAtaaaataaaa                                                                                     <  2:826789/141‑1 (MQ=255)
                                                        ccTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAAc                                                                                    <  2:180508/141‑1 (MQ=255)
                                                         cTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACa                                                                                   <  2:329421/141‑1 (MQ=255)
                                                         cTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACa                                                                                   <  3:107377/141‑1 (MQ=255)
                                                          tACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACaa                                                                                  <  4:420517/141‑1 (MQ=255)
                                                           aCCAATATAGACTTAGTAATTCGATTTTTAATACATTACATTCCTAAAATAAATACATCCATCATAATACTTCTCAAATGTCTTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAg                                                                                 <  4:46837/141‑1 (MQ=255)
                                                            ccAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTACATACAGCACAAATAAACATTTTTAAATATATAAAATAAAACAAGa                                                                                >  3:225402/1‑141 (MQ=255)
                                                              aaTATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATa                                                                              >  1:1017898/1‑141 (MQ=255)
                                                               atatatACTTAGTAATTCGATTGTTAATACATCACTTTCATAAAATAACTATAACCATCATAATACTTCTCAAATCTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATAc                                                                             <  2:484242/141‑1 (MQ=255)
                                                               atatAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATAc                                                                             >  1:297008/1‑141 (MQ=255)
                                                                 ataGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTc                                                                           <  2:240360/141‑1 (MQ=255)
                                                                  taGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCa                                                                          >  3:261149/1‑141 (MQ=255)
                                                                   ataCTTAGTAATTCGATTGTTAATACATCACTTTCATAAAATAACTATAACCATCATAATACTTCTCAAATCTCGTCAAATTAAAGTAAATACGGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACtaca                                                                         <  1:478743/139‑4 (MQ=255)
                                                                   aGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCaa                                                                         >  3:84723/1‑141 (MQ=255)
                                                                   aGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCaa                                                                         <  1:453197/141‑1 (MQ=255)
                                                                      cTTAGTAATTCGATTGTTAATACATCACTTTCATAAAATAACTATAACCATCATAATACTTCTCAAATCTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCaataa                                                                      >  2:671660/1‑141 (MQ=255)
                                                                        tAGTAATTCGATTGTTAATACATTACTTTCATAGAATAGCTACGACCATCATAATAGTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATc                                                                    <  1:644260/141‑1 (MQ=255)
                                                                                cGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTAc                                                            <  1:371647/141‑1 (MQ=255)
                                                                                cGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTCCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTAc                                                            <  1:695818/141‑1 (MQ=255)
                                                                                  aTTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCt                                                          <  3:273495/141‑1 (MQ=255)
                                                                                  aTTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATCAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCt                                                          >  4:315769/1‑141 (MQ=255)
                                                                                     gTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGt                                                       <  2:1042032/141‑1 (MQ=255)
                                                                                        aaTACATCACTTTCATAAAATAACTATAACCATCATAATACTTCTCAAATCTCGTCAAAATAAAGAAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACAAAATAATATGTAGTATCTTGtttt                                                    >  3:121214/1‑141 (MQ=255)
                                                                                           aCATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGa                                                 >  1:4469/1‑141 (MQ=255)
                                                                                             aTTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGaat                                               <  4:227670/141‑1 (MQ=255)
                                                                                                 cTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATg                                           <  2:33292/141‑1 (MQ=255)
                                                                                                  tttCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGt                                          <  3:336960/141‑1 (MQ=255)
                                                                                                    tCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTaa                                        >  2:958232/1‑141 (MQ=255)
                                                                                                    tCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTaa                                        >  2:442035/1‑141 (MQ=255)
                                                                                                     cATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAAt                                       <  2:720922/141‑1 (MQ=255)
                                                                                                      aTAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAAtt                                      >  1:395875/1‑141 (MQ=255)
                                                                                                         aaaTAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTta                                   <  1:330212/141‑1 (MQ=255)
                                                                                                           aTAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTtata                                 >  1:731298/1‑141 (MQ=255)
                                                                                                           aTAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTtata                                 >  3:396697/1‑141 (MQ=255)
                                                                                                           aTAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTtata                                 >  4:101979/1‑141 (MQ=255)
                                                                                                            tAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTatat                                <  1:1006269/141‑1 (MQ=255)
                                                                                                             aaCTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATatc                               <  2:1067895/141‑1 (MQ=255)
                                                                                                               cTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATatcat                             >  1:868818/1‑141 (MQ=255)
                                                                                                                 aCAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGAAAATACAGCACACATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATAtcatca                           <  2:933889/141‑1 (MQ=255)
                                                                                                                      catcatAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAGATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGc                      <  2:952838/141‑1 (MQ=255)
                                                                                                                      catcatAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGc                      <  1:802886/141‑1 (MQ=255)
                                                                                                                        tcatAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTa                    >  1:197275/1‑141 (MQ=255)
                                                                                                                         catAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTAc                   <  2:552808/141‑1 (MQ=255)
                                                                                                                          ataataCTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACa                  <  1:735921/141‑1 (MQ=255)
                                                                                                                           taataCTTCTCAAATCTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTATCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTTCaa                 <  1:224328/141‑1 (MQ=255)
                                                                                                                            aataCTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACaaa                >  1:648525/1‑141 (MQ=255)
                                                                                                                              taCTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATg              <  2:189177/141‑1 (MQ=255)
                                                                                                                               aCTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGa             <  1:508426/141‑1 (MQ=255)
                                                                                                                               aCTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGa             <  4:400922/141‑1 (MQ=255)
                                                                                                                                 ttCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGc           <  2:860160/141‑1 (MQ=255)
                                                                                                                                   ctcAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAg         <  4:261149/141‑1 (MQ=255)
                                                                                                                                    tcAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGt        >  3:15606/1‑141 (MQ=255)
                                                                                                                                     cAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGtt       <  2:618811/141‑1 (MQ=255)
                                                                                                                                     cAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGtt       <  2:618470/141‑1 (MQ=255)
                                                                                                                                     aaaaTGTAGTCAAAGTAAAGTATAAACAGCACAAATTAACATTTTTAAATATATAATATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACTGCTACATATGAGCAGtt       <  4:225402/140‑1 (MQ=255)
                                                                                                                                      aaaTGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGttt      <  3:166742/141‑1 (MQ=255)
                                                                                                                                       aaTGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTc     >  1:368454/1‑141 (MQ=255)
                                                                                                                                        aTGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACTTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCa    <  2:945615/141‑1 (MQ=255)
                                                                                                                                          gtcgtcAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCata  >  2:235098/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CATCTCCATATTAAAAACTTAAATAGTGCATTCCATTTAAACTAAACATCGATATTCCTACCAATATAGACTTAGTAATTCGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATA  >  USA300TCH1516_ALE/1345972‑1346250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: