BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,354,040	C→T	5.7%	S255S (TCG→TCA)	USA300HOU_RS06735 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,354,040	0	C	T	5.7%	410.1 / 8.9	131	S255S (TCG→TCA)	USA300HOU_RS06735	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (65/57); new base T (6/2); total (72/59)
Fisher's exact test for biased strand distribution p-value = 2.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TAATAACCATAGTGCGTATCGAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAA  >  USA300TCH1516_ALE/1353901‑1354180

taataaCCATAGTGCGTATCGAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACg                                                                                                                                             <  4:293534/141‑1 (MQ=255)

 aataaCCATAGTGCGTATCGAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGa                                                                                                                                            <  2:174574/141‑1 (MQ=255)

 aataaCCATAGTGCGTATCGAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGa                                                                                                                                            <  1:650285/141‑1 (MQ=255)

  ataaCCATAGTGCGTATCGAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGaa                                                                                                                                           <  4:440329/141‑1 (MQ=255)

  ataaCCATAGTGCGTATCGAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGaa                                                                                                                                           <  4:419835/141‑1 (MQ=255)

  ataaCCATAGTGCGTATCGAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATATGATCAACCTGCTCTTTATTTAAAGCGTGTTTAGATAGCACTATGGCTTCGCTAATAATACGTCGTAACGaa                                                                                                                                           <  1:215271/141‑1 (MQ=255)

   taaCCATAGTGCGTATCGAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAg                                                                                                                                          >  1:155560/1‑141 (MQ=255)

    aaCCATAGTGCGTATCGAGCGTAATGGATTATTATTCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACTTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTTATATTACGTCGTTACGAAGa                                                                                                                                         >  4:362283/1‑141 (MQ=255)

    aaCCATAGTGCGTATCGAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGa                                                                                                                                         >  1:983677/1‑141 (MQ=255)

    aaCCATAGTGCGTATCGAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGa                                                                                                                                         <  1:598160/141‑1 (MQ=255)

          aGTGCGTATCGAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTttgt                                                                                                                                   <  1:991795/141‑1 (MQ=255)

                      gCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATc                                                                                                                       <  2:453621/141‑1 (MQ=255)

                       cGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCa                                                                                                                      >  2:329828/1‑141 (MQ=255)

                          aaTGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACa                                                                                                                   <  3:415012/141‑1 (MQ=255)

                          aaTGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACa                                                                                                                   <  1:749667/141‑1 (MQ=255)

                             ggATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACatat                                                                                                                >  1:710376/1‑141 (MQ=255)

                             ggATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACatat                                                                                                                >  1:769587/1‑141 (MQ=255)

                               attattATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATAttt                                                                                                              <  2:504823/141‑1 (MQ=255)

                                  attatGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTAtc                                                                                                           <  3:326317/141‑1 (MQ=255)

                                  attatGCCTTTGATTTTACTTGAATATAGCTGCGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTAtc                                                                                                           >  2:854197/1‑141 (MQ=255)

                                   ttatGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTAtct                                                                                                          <  2:223299/141‑1 (MQ=255)

                                      tGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCtctt                                                                                                       >  2:527197/1‑141 (MQ=255)

                                      tGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCtctt                                                                                                       >  4:119357/1‑141 (MQ=255)

                                           ttGATTTTACTTGAATATAGCTGTGATTTTATCAACCTGTTTTTTTTTTTAAGGGTGTTTTGATAGCTCTATGGCTTCTCTAATAATACGTTGTAAAGAAGATTTTGTTTTAACTGTATCAACATATTTATTtctttcttt                                                                                                  >  2:243909/1‑140 (MQ=255)

                                             gATTTTACTTGAATATAGCTGTGATTTGATCAAGCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAg                                                                                                <  4:345663/141‑1 (MQ=255)

                                             gATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAg                                                                                                <  2:19966/141‑1 (MQ=255)

                                              aTTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGa                                                                                               <  1:863948/141‑1 (MQ=255)

                                              aTTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGa                                                                                               >  3:156865/1‑141 (MQ=255)

                                              aTTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGa                                                                                               <  1:1020904/141‑1 (MQ=255)

                                               ttttACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGaa                                                                                              >  1:740789/1‑141 (MQ=255)

                                                 ttACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAAcc                                                                                            >  1:962893/1‑141 (MQ=255)

                                                  tACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCt                                                                                           <  2:904129/141‑1 (MQ=255)

                                                  tACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCt                                                                                           >  3:327682/1‑141 (MQ=255)

                                                  tACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCt                                                                                           <  1:927287/141‑1 (MQ=255)

                                                   aCTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCtt                                                                                          >  2:791925/1‑141 (MQ=255)

                                                   aCTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCtt                                                                                          >  1:128189/1‑141 (MQ=255)

                                                       gAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAAt                                                                                      <  1:1023827/141‑1 (MQ=255)

                                                       gAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAAt                                                                                      >  1:316231/1‑141 (MQ=255)

                                                          tataGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGat                                                                                   <  2:62893/141‑1 (MQ=255)

                                                              gCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTa                                                                               >  1:678647/1‑141 (MQ=255)

                                                              gCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTa                                                                               >  1:840579/1‑141 (MQ=255)

                                                              gCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTa                                                                               >  1:738405/1‑141 (MQ=255)

                                                              gCTGTGATTTGATCAACCTGTTATTTGTTAAAAGGGGGTTTAGATAGCTCTATGGCTCCCCTAATAAGACGCCGGAATGAAAATTTTGTTGCAACGTAACCAACATAGTTATCTCTTCTCTCAGAACCTAGACTGATATTa                                                                               >  4:197082/1‑141 (MQ=255)

                                                               cTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTaa                                                                              <  4:242965/141‑1 (MQ=255)

                                                               cTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTaa                                                                              <  2:853435/141‑1 (MQ=255)

                                                                tgtgATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAAc                                                                             >  2:658203/1‑141 (MQ=255)

                                                                  tgATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTg                                                                           <  1:44163/141‑1 (MQ=255)

                                                                    aTTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTa                                                                         >  3:341515/1‑141 (MQ=255)

                                                                      ttGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTAcc                                                                       <  1:471591/141‑1 (MQ=255)

                                                                       tGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCa                                                                      >  1:915953/1‑141 (MQ=255)

                                                                        gATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCAt                                                                     >  1:184410/1‑141 (MQ=255)

                                                                          tCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCa                                                                   <  4:29204/141‑1 (MQ=255)

                                                                           cAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCAc                                                                  >  3:116567/1‑141 (MQ=255)

                                                                            aaCCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACt                                                                 <  2:954802/141‑1 (MQ=255)

                                                                              ccTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTaa                                                               <  2:360730/141‑1 (MQ=255)

                                                                               cTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTATATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTaaa                                                              <  2:1009347/141‑1 (MQ=255)

                                                                                 gTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGt                                                            >  4:54101/1‑141 (MQ=255)

                                                                                 gTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGt                                                            >  2:958970/1‑141 (MQ=255)

                                                                                   tCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAATGAAGATTTTGTTGAAACGGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTaa                                                          >  1:662429/1‑141 (MQ=255)

                                                                                   tCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTaa                                                          >  1:595478/1‑141 (MQ=255)

                                                                                      ttatttaAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATctct                                                                                                        <  2:634130/92‑1 (MQ=255)

                                                                                      ttatttaAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATctct                                                                                                        >  1:634130/1‑92 (MQ=255)

                                                                                         tttaAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAaca                                                    <  1:795188/141‑1 (MQ=255)

                                                                                         tttaAAGAGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAaca                                                    >  3:402492/1‑141 (MQ=255)

                                                                                          ttaAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAacac                                                   >  4:37440/1‑141 (MQ=255)

                                                                                           taAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACAcc                                                  >  2:569794/1‑141 (MQ=255)

                                                                                             aaGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCgg                                                >  1:700924/1‑141 (MQ=255)

                                                                                             aaGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACAgg                                                >  1:223099/1‑141 (MQ=255)

                                                                                             aaGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGGCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCgg                                                >  2:663604/1‑141 (MQ=255)

                                                                                              aGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGt                                               >  1:438756/1‑141 (MQ=255)

                                                                                              aGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGt                                               >  2:314578/1‑141 (MQ=255)

                                                                                              aGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGt                                               >  2:940550/1‑141 (MQ=255)

                                                                                              aGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGt                                               >  2:797227/1‑141 (MQ=255)

                                                                                               gggTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTa                                              >  4:224888/1‑141 (MQ=255)

                                                                                                ggTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAATGAAGATTTTGTTGAAACGGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTAc                                             >  1:54963/1‑141 (MQ=255)

                                                                                                  tgtTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACtt                                           >  2:379795/1‑141 (MQ=255)

                                                                                                  tgtTTAGATAGCTCTANGGCTTCGCTAATNATACGTCGTAACGAAGATTTTGTTGAAACTGTATCTACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACtt                                           >  1:759365/1‑141 (MQ=255)

                                                                                                   gtTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACttt                                          >  4:450335/1‑141 (MQ=255)

                                                                                                    ttttGATAGGTCTATGGCTTAGCTAAATATACGGCGTAACGAAGGTTTTGTTGATACTGTATCAACATATTTATCTCTTTCTTCTTAACCTTGAGTGATATTAACTATACCATCCCTAATGTACATAACACCGGTACTTTg                                         <  4:132319/141‑1 (MQ=255)

                                                                                                    tttAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTg                                         <  2:393991/141‑1 (MQ=255)

                                                                                                    tttAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTg                                         <  3:126208/141‑1 (MQ=255)

                                                                                                    tttAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTg                                         <  2:999376/141‑1 (MQ=255)

                                                                                                     ttAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTgg                                        <  2:1054304/141‑1 (MQ=255)

                                                                                                         aTAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACtt                                    >  4:404642/1‑141 (MQ=255)

                                                                                                                 aTGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTttaaagtta                            <  1:985984/141‑1 (MQ=255)

                                                                                                                  tGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTttaaagttaa                           >  3:11288/1‑141 (MQ=255)

                                                                                                                   ggCTTCGCTAATAATACGTCGTAATGAAGATTTTGTTGAAACGGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTttaaagttaaa                          >  1:264053/1‑141 (MQ=255)

                                                                                                                      ttCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTaaagttaaagtt                       <  2:176439/141‑1 (MQ=255)

                                                                                                                       tCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTTTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGttt                      <  1:550205/141‑1 (MQ=255)

                                                                                                                       tCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGttt                      <  1:718907/141‑1 (MQ=255)

                                                                                                                          cTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTCCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGt                   >  1:891431/1‑141 (MQ=255)

                                                                                                                            aataatACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTc                 >  3:93478/1‑141 (MQ=255)

                                                                                                                             ataataCGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGGACCATCACTAAAGTAAATAACACCGGTACTTTAGACTTTAAAGTTAAAGTTTTGTTCa                <  3:424139/141‑1 (MQ=255)

                                                                                                                               aataCGTCGTAACGAAGATTTTGTTGAAGCTGTATCAACATATTTATCTCTTTCTTCAGAACCGTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAATTTTTGTTCAat              <  3:223316/141‑1 (MQ=255)

                                                                                                                               aataCGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAat              >  1:193687/1‑141 (MQ=255)

                                                                                                                                ataCGTCTTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAata             >  4:220935/1‑141 (MQ=255)

                                                                                                                                          aCGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTa   >  1:931133/1‑141 (MQ=255)

                                                                                                                                          aCGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTa   >  1:486142/1‑141 (MQ=255)

                                                                                                                                           cGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCACTATGATCCTTTaa  >  2:1024225/1‑141 (MQ=255)

                                                                                                                                           cGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTaa  >  4:39123/1‑141 (MQ=255)

TAATAACCATAGTGCGTATCGAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAA  >  USA300TCH1516_ALE/1353901‑1354180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`