Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,390,631 T→G 5.7% intergenic (+115/‑135) parC → / → alsT DNA topoisomerase 4 subunit A/Amino‑acid carrier protein AlsT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,390,6310TG 5.7% 316.1 / 11.7 105intergenic (+115/‑135)parC/alsTDNA topoisomerase 4 subunit A/Amino‑acid carrier protein AlsT
Reads supporting (aligned to +/- strand):  ref base T (47/52);  new base G (3/3);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 108 total aligned reads displayed.

TAATAGACATGTATATTAGCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAGGGGTTTATTTTGAAA  >  USA300TCH1516_ALE/1390494‑1390771
                                                                                                                                         |                                                                                                                                            
tAATAGACATGTATATTAGCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACt                                                                                                                                           <  2:531446/141‑1 (MQ=255)
 aaTAGACATGTATATTAGCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACtt                                                                                                                                          <  2:664586/141‑1 (MQ=255)
    aGACATGTATATTAGCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAAGGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGa                                                                                                                                       >  3:225894/1‑141 (MQ=255)
    aGAAATGTATATTATCTAAAAACTAGATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGTTATTTATATTATATGCTGACCAATTACTTATCGTTAAATTAGCGATATACtttta                                                                                                                                       <  4:223180/141‑3 (MQ=255)
     gACATGTATATTAGCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGAc                                                                                                                                      <  2:1066937/141‑1 (MQ=255)
       cATGTATATTAGCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCa                                                                                                                                    <  1:503302/141‑1 (MQ=255)
        aTGTATATTAGCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCaa                                                                                                                                   <  1:991273/141‑1 (MQ=255)
          gTATATTAGCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAAc                                                                                                                                 >  1:680842/1‑141 (MQ=255)
          gTATATTAGCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAAc                                                                                                                                 >  1:831498/1‑141 (MQ=255)
              atTAGCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAat                                                                                                                             >  2:734540/1‑141 (MQ=255)
                tatcTAAAAACGATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAatat                                                                                                                           <  2:741548/138‑1 (MQ=255)
                 aGCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAAtata                                                                                                                          >  1:902085/1‑141 (MQ=255)
                 aGCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAAtata                                                                                                                          >  1:129584/1‑141 (MQ=255)
                  gCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATaa                                                                                                                         >  1:306608/1‑141 (MQ=255)
                  gCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATaa                                                                                                                         >  1:113767/1‑141 (MQ=255)
                   cTAAAAACTATATGCAATCTCGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATaaa                                                                                                                        <  2:759243/141‑1 (MQ=255)
                     aaaaaCTATATGCAATCACGAAATTAAATAATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAAtc                                                                                                                      <  2:526697/141‑1 (MQ=255)
                      aaaaCTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAAtct                                                                                                                     >  1:535652/1‑141 (MQ=255)
                      aaaaCTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAAtct                                                                                                                     <  3:28009/141‑1 (MQ=255)
                       aaaCTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAAtctc                                                                                                                    >  1:927505/1‑141 (MQ=255)
                       aaaCTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAAtctc                                                                                                                    <  2:701184/141‑1 (MQ=255)
                        aaCTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATctct                                                                                                                   >  2:147907/1‑141 (MQ=255)
                        aaCTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATctct                                                                                                                   <  1:899875/141‑1 (MQ=255)
                        aaCTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATctct                                                                                                                   <  3:242602/141‑1 (MQ=255)
                         aCTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCtt                                                                                                                  <  2:365886/141‑1 (MQ=255)
                         aCTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCtt                                                                                                                  <  2:550558/141‑1 (MQ=255)
                         aCTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCtt                                                                                                                  >  3:375796/1‑141 (MQ=255)
                            atatGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCttgta                                                                                                               >  2:623209/1‑141 (MQ=255)
                            atatGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCttgta                                                                                                               >  2:958793/1‑141 (MQ=255)
                            atatGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCttgta                                                                                                               <  2:677515/141‑1 (MQ=255)
                            atatGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTAAAGGGATTTATATTAAAGGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTTATATAAATCTCtttga                                                                                                               >  2:264567/1‑138 (MQ=255)
                               tGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCttgtattg                                                                                                            <  1:18294/141‑1 (MQ=255)
                               tGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCttgtattg                                                                                                            >  3:18718/1‑141 (MQ=255)
                               tGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTAGCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCttgtattg                                                                                                            <  3:349465/141‑1 (MQ=255)
                                   aTCAAGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAAAGCTGACCAAGTACTTATCGTTGCATTAGCGATATACTTTGACCACACTAATATAAATCTCTTGTATTGtata                                                                                                        <  1:189034/141‑1 (MQ=255)
                                    tCACGAAATTAATTGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATGAATCTCTTGTATTGTATaa                                                                                                       >  2:467749/1‑141 (MQ=255)
                                          aaTTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAatg                                                                                                 >  4:19493/1‑141 (MQ=255)
                                                gtGATAAAATACGGTAAAGTTACGTTATGACGAAGTTCAAGAGAATTATATTAAATGCTGCCCAAGTACTTATCGTTAAATTAGCGATATACTTTGTCCAAACTAATATAAAACTCTTGAAATGTATAAAAAatgatgttg                                                                                           >  4:243161/2‑141 (MQ=255)
                                                aTGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAatgatgatg                                                                                           <  1:33836/141‑1 (MQ=255)
                                                 tGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGt                                                                                          >  4:217206/1‑141 (MQ=255)
                                                   aTAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTAt                                                                                        <  1:632784/141‑1 (MQ=255)
                                                     aaaaTACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTAtct                                                                                      >  3:168836/1‑141 (MQ=255)
                                                        aTACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCta                                                                                   >  4:451715/1‑141 (MQ=255)
                                                              tAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTa                                                                             <  2:256629/141‑1 (MQ=255)
                                                              tAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATAGACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTa                                                                             <  2:796316/141‑1 (MQ=255)
                                                              tAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATAGACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTa                                                                             <  2:796240/141‑1 (MQ=255)
                                                                  gTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGa                                                                         <  1:358346/141‑1 (MQ=255)
                                                                     aaaTTTTGACTAAATTCAAGTGATTTATATTAAATGCTGACCAAGTACGTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAAGGATGATGTATCTCTATATTTACTGAAgg                                                                      >  2:919312/1‑141 (MQ=255)
                                                                     aaaTTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTGTATATTTACTGAAgg                                                                      >  1:910876/1‑141 (MQ=255)
                                                                     aaaTTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATAGACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAgt                                                                      <  3:246108/141‑2 (MQ=255)
                                                                          ttGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCaa                                                                 >  2:879642/1‑141 (MQ=255)
                                                                           tGACTAAATTCAAGGGATTTATATTAAATGCTGATCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAAt                                                                <  1:1005044/141‑1 (MQ=255)
                                                                            gACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAAtt                                                               >  1:887519/1‑141 (MQ=255)
                                                                             aCTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGACATGATGATGTATCTCTATATTTACTGAAGGTTCAATTc                                                              <  1:572055/141‑1 (MQ=255)
                                                                             aCTAAATTCAAGGGACTCATATGAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTc                                                              <  1:9461/141‑1 (MQ=255)
                                                                                aaaTTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGt                                                           >  3:401015/1‑141 (MQ=255)
                                                                                aaaTTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGt                                                           <  2:497621/141‑1 (MQ=255)
                                                                                aaaTTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGt                                                           <  3:453694/141‑1 (MQ=255)
                                                                                 aaTTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTc                                                          <  1:958793/141‑1 (MQ=255)
                                                                                 aaTTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTc                                                          <  1:479528/141‑1 (MQ=255)
                                                                                 aaTTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTc                                                          <  2:442251/141‑1 (MQ=255)
                                                                                     ccaGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAAt                                                      <  1:376866/139‑1 (MQ=255)
                                                                                       aGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAAttt                                                    >  1:2291/1‑141 (MQ=255)
                                                                                        gggATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAAtttt                                                   >  2:2515/1‑141 (MQ=255)
                                                                                          gATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTtat                                                 <  2:801693/141‑1 (MQ=255)
                                                                                          gATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTtat                                                 <  1:992780/141‑1 (MQ=255)
                                                                                          gATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATAGACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGTTTCAATTCGGTCTAATTTtat                                                 >  1:649007/1‑141 (MQ=255)
                                                                                                 atTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAATGTTCAATTCGGTCTAATTTTATAGAAGGt                                          >  2:895029/1‑141 (MQ=255)
                                                                                                   tAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTAc                                        >  2:481778/1‑141 (MQ=255)
                                                                                                   tAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTAc                                        <  3:29619/141‑1 (MQ=255)
                                                                                                    aaaTGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACa                                       <  3:454142/141‑1 (MQ=255)
                                                                                                    aaaTGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACa                                       <  1:669140/141‑1 (MQ=255)
                                                                                                         cTGACCAAGTACTTATCGTTAAATTAGCGATAGACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGAGGTATCTCTATATTTACTGAAGTTTCAATTCGGTCTAATTTTATAGAAGGTGCATGAtt                                  >  2:355367/1‑141 (MQ=255)
                                                                                                           gACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCa                                >  4:159942/1‑141 (MQ=255)
                                                                                                            aacAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGAATCAt                               <  1:956651/139‑1 (MQ=255)
                                                                                                            aCCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCAt                               >  2:656984/1‑141 (MQ=255)
                                                                                                             ccAAGTACTTATCGTTAAATTAGCGATATCCTTTAACCGAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATg                              >  1:575183/1‑141 (MQ=255)
                                                                                                             accaGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATg                              >  4:50890/4‑141 (MQ=255)
                                                                                                              cAAGTACTTATCGTTAAATTAGCGATATACTTTGACCACACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGt                             >  1:575544/1‑141 (MQ=255)
                                                                                                              cAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGt                             >  1:206905/1‑141 (MQ=255)
                                                                                                                 gTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTaga                          <  2:80754/141‑1 (MQ=255)
                                                                                                                  tACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTagag                         <  2:37072/141‑1 (MQ=255)
                                                                                                                   aCTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAgg                        <  2:902085/141‑1 (MQ=255)
                                                                                                                    cTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGa                       <  2:937252/141‑1 (MQ=255)
                                                                                                                     ttATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGAt                      <  1:824894/141‑1 (MQ=255)
                                                                                                                       aTCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGc                    >  3:439045/1‑141 (MQ=255)
                                                                                                                          gTTATATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAg                 <  1:500233/141‑1 (MQ=255)
                                                                                                                             aaaTTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAgggg              >  3:360454/1‑141 (MQ=255)
                                                                                                                             aaaTTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAgggg              <  2:680842/141‑1 (MQ=255)
                                                                                                                              aaTTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAGGGGt             <  1:94965/141‑1 (MQ=255)
                                                                                                                              aaTTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAGGGGt             >  3:116989/1‑141 (MQ=255)
                                                                                                                               aTTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAGGGGtt            <  1:309996/141‑1 (MQ=255)
                                                                                                                                ttAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAGGGGttt           <  2:831498/141‑1 (MQ=255)
                                                                                                                                  aGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAGGGGTTTAt         >  4:159756/1‑141 (MQ=255)
                                                                                                                                   gCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAGGGGTTTAtt        >  1:678375/1‑141 (MQ=255)
                                                                                                                                    cGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAGGGGTTTAttt       >  2:548003/1‑141 (MQ=255)
                                                                                                                                    cGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAGGGGTTTAttt       >  3:255824/1‑141 (MQ=255)
                                                                                                                                     gATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAGGGGTTTAtttt      <  3:58814/141‑1 (MQ=255)
                                                                                                                                       tataCTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAGGGGTTTATTTTGa    >  4:303702/1‑141 (MQ=255)
                                                                                                                                         taCTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAGGGGTTTATTTTGaaa  >  1:1021649/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                            
TAATAGACATGTATATTAGCTAAAAACTATATGCAATCACGAAATTAAATGATAAAATACAGTAATGTTAAATTTTGACTAAATTCAAGGGATTTATATTAAATGCTGACCAAGTACTTATCGTTAAATTAGCGATATACTTTGACCAAACTAATATAAATCTCTTGTATTGTATAAGAAATGATGATGTATCTCTATATTTACTGAAGGTTCAATTCGGTCTAATTTTATAGAAGGTACATGATTCATGTAGAGGATGCGAGGGGTTTATTTTGAAA  >  USA300TCH1516_ALE/1390494‑1390771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: