Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,450,291 G→A 5.4% D273D (GAC→GAT)  odhB ← Dihydrolipoyllysine‑residue succinyltransferase component of 2‑oxoglutarate dehydrogenase complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,450,2910GA 5.4% 350.3 / 5.6 112D273D (GAC→GAT) odhBDihydrolipoyllysine‑residue succinyltransferase component of 2‑oxoglutarate dehydrogenase complex
Reads supporting (aligned to +/- strand):  ref base G (54/51);  new base A (3/3);  total (57/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 117 total aligned reads displayed.

ACTGCTAAATTAGCAATTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGCAATTCCATAACATT  >  USA300TCH1516_ALE/1450151‑1450431
                                                                                                                                            |                                                                                                                                            
aCTGCTAAATTAGCAATTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCg                                                                                                                                              <  4:137648/141‑1 (MQ=255)
aCTGCTAAATTAGCAATTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCg                                                                                                                                              >  1:397878/1‑141 (MQ=255)
  tGCTAAATTAGCAATTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTc                                                                                                                                            <  2:188698/141‑1 (MQ=255)
  tGCTAAATTAGCAATTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTc                                                                                                                                            <  2:189300/141‑1 (MQ=255)
   gCTAAATTAGCAATTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCg                                                                                                                                           <  2:81115/141‑1 (MQ=255)
     tAAATTAGCAATTTCTGCTTCGATGTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACCGCTACCCCTATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGAt                                                                                                                                         <  4:386314/141‑1 (MQ=255)
        aTTAGCAATTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTc                                                                                                                                      >  3:73360/1‑141 (MQ=255)
        aTTAGCAATTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTc                                                                                                                                      <  3:397683/141‑1 (MQ=255)
         ttAGCAATTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCt                                                                                                                                     >  1:299929/1‑141 (MQ=255)
           aGCAATTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGc                                                                                                                                   >  1:692600/1‑141 (MQ=255)
            gCAATTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCa                                                                                                                                  <  2:550511/141‑1 (MQ=255)
            gCAATTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCa                                                                                                                                  <  3:290546/141‑1 (MQ=255)
               aTTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTa                                                                                                                               <  2:950902/141‑1 (MQ=255)
                tttCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTaa                                                                                                                              <  3:362741/141‑1 (MQ=255)
                  tCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACt                                                                                                                            <  4:58269/141‑1 (MQ=255)
                     gCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCt                                                                                                                         <  2:500897/141‑1 (MQ=255)
                      cTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTg                                                                                                                        >  3:131112/1‑141 (MQ=255)
                       ttCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTgg                                                                                                                       <  4:39390/141‑1 (MQ=255)
                       ttCGAATTCTGCAATATTCTTTTTATCACAATCTCTTACAAATGGTACTAATTATCCATCATCTGTAGAAACAGCTACACCAATCTCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTgg                                                                                                                       <  4:314317/141‑1 (MQ=255)
                        tCGATTTCTGCAAAATTCTTTTTTTTTTTTTTTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATTTTGTTTCGTAATCATGTCGTCGCCATCGATTTCTACTTTTtcttcttgt                                                                                                                      >  2:602628/1‑138 (MQ=255)
                              tCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACttt                                                                                                                <  1:353044/141‑1 (MQ=255)
                               cTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACtttt                                                                                                               >  3:60319/1‑141 (MQ=255)
                                tGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACttttt                                                                                                              <  2:920834/141‑1 (MQ=255)
                                tGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACttttt                                                                                                              >  2:892010/1‑141 (MQ=255)
                                tGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACttttt                                                                                                              >  2:587311/1‑141 (MQ=255)
                                  cAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACTCCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCa                                                                                                            <  2:481370/141‑1 (MQ=255)
                                         cTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAgctgc                                                                                                     >  1:320724/1‑141 (MQ=255)
                                         cTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAgctgc                                                                                                     <  2:240265/141‑1 (MQ=255)
                                         cTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCATCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAgctgc                                                                                                     >  1:706127/1‑141 (MQ=255)
                                           ttttATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTa                                                                                                   <  2:58386/141‑1 (MQ=255)
                                            tttATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTAc                                                                                                  >  2:64064/1‑141 (MQ=255)
                                             ttATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACa                                                                                                 <  1:817002/141‑1 (MQ=255)
                                             ttATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCATCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACa                                                                                                 <  1:668434/141‑1 (MQ=255)
                                              tATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAg                                                                                                <  4:195427/141‑1 (MQ=255)
                                                  ccaATCTCTGACAAGTGGTACTAATAATCCATTATCTGTAGAAACAGCTACACCAATATCAGAATATTGTTTCGTAATCAGAACGTCGCCTCCGATTTCTGCATTAACTTCTGGATTCTTTTTCAAAGCTTCTCCATAAGc                                                                                            <  4:19763/140‑1 (MQ=255)
                                                  acaGTCTCTTAAAGATTGTACTAGTAATCGATCATCTGTAGAATCAGCTACACCACTATCATAATATTGTTTCGTAATCATGTCGTCGCCGGCGATTTCTACACTGACTTCTAGATACTTTTTCAAAGCTGCTACAGAAGc                                                                                            <  2:463219/141‑1 (MQ=255)
                                                   caATCTCTTACAAATGGTACTAATAATCCATCATCTTTAGAAACAGCTACACCTTTTTCATATTATTGTTTTTTAATTATGTCGTCGCCGTCTATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCt                                                                                           >  3:409852/1‑141 (MQ=255)
                                                   caATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCt                                                                                           >  1:247380/1‑141 (MQ=255)
                                                        tctTACGAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGt                                                                                      <  2:710415/141‑1 (MQ=255)
                                                        tctTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGt                                                                                      <  4:33306/141‑1 (MQ=255)
                                                        tctTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGt                                                                                      <  4:346990/141‑1 (MQ=255)
                                                         ctTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCTATGTTGCTACAGAAGCTTTAGTa                                                                                     >  2:663961/1‑141 (MQ=255)
                                                          ttACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTaa                                                                                    <  3:102121/141‑1 (MQ=255)
                                                           tACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTaaa                                                                                   <  2:284759/141‑1 (MQ=255)
                                                           tACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTaaa                                                                                   >  4:288897/1‑141 (MQ=255)
                                                           tACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTaaa                                                                                   <  4:207493/141‑1 (MQ=255)
                                                           tACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTaaa                                                                                   <  2:640466/141‑1 (MQ=255)
                                                            aCAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAg                                                                                  >  1:960139/1‑141 (MQ=255)
                                                             cAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGa                                                                                 >  4:354172/1‑141 (MQ=255)
                                                              aaaTGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGaa                                                                                <  2:1030858/141‑1 (MQ=255)
                                                               aaTGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAAt                                                                               >  2:261874/1‑141 (MQ=255)
                                                                aTGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATCCTTTTTCCTGTCTGCTTCAGAAGCTTTAGTAAAGAATg                                                                              <  1:253024/141‑1 (MQ=255)
                                                                aTGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATg                                                                              <  1:938231/141‑1 (MQ=255)
                                                                 tGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGa                                                                             >  1:1065179/1‑141 (MQ=255)
                                                                   gTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACa                                                                           >  1:680480/1‑141 (MQ=255)
                                                                     aCTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATa                                                                         >  4:274732/1‑141 (MQ=255)
                                                                        aataatCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAAt                                                                      <  4:284004/141‑1 (MQ=255)
                                                                        aataatCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAAt                                                                      >  3:18936/1‑141 (MQ=255)
                                                                         ataatCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATc                                                                     >  3:47865/1‑141 (MQ=255)
                                                                          taatCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATcc                                                                    <  1:1038028/141‑1 (MQ=255)
                                                                              ccATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAAt                                                                <  2:1031037/141‑1 (MQ=255)
                                                                              ccATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAAt                                                                >  1:152290/1‑141 (MQ=255)
                                                                              ccATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAAt                                                                >  2:176471/1‑141 (MQ=255)
                                                                                atcatcTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAAttt                                                              <  1:394348/141‑1 (MQ=255)
                                                                                 tcatcTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTa                                                             <  1:789651/141‑1 (MQ=255)
                                                                                  catcTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATGATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAATAATGACATAAATCCTAATTTAg                                                            >  3:378623/1‑141 (MQ=255)
                                                                                  catcTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAg                                                            >  2:992462/1‑141 (MQ=255)
                                                                                  catcTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAg                                                            >  3:75769/1‑141 (MQ=255)
                                                                                         aGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTTCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACcatc                                                     >  4:281354/1‑141 (MQ=255)
                                                                                            aaCAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATg                                                  >  3:424807/1‑141 (MQ=255)
                                                                                             aCAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGa                                                 <  2:397878/141‑1 (MQ=255)
                                                                                                   acacCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTc                                           >  2:50287/1‑141 (MQ=255)
                                                                                                    cacCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCa                                          >  2:493676/1‑141 (MQ=255)
                                                                                                      ccAATATCATAATATTGTTTCGTAATCATTTCGTCTCTGTCGTTTTTTGCATTAACTTCTGGATACTTTTTCATAGCTGCTACAGAAGTTTTATTAAAGTATGACATAATTTCTTATTTAGTATCTTCTTGATTTTTCAtt                                        >  2:568297/1‑140 (MQ=255)
                                                                                                        aaTATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATaaa                                      >  2:87855/1‑141 (MQ=255)
                                                                                                         atatCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAAt                                     <  1:105469/141‑1 (MQ=255)
                                                                                                           atCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTg                                   >  1:448207/1‑141 (MQ=255)
                                                                                                            tCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTTGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGt                                  >  1:566910/1‑141 (MQ=255)
                                                                                                            tCATAATATTGTTTCGTAATCATGTCGTCGCCATCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGt                                  <  2:706127/141‑1 (MQ=255)
                                                                                                              ataataTTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGttc                                >  2:227553/1‑141 (MQ=255)
                                                                                                              ataataTTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGttc                                <  2:767633/141‑1 (MQ=255)
                                                                                                              ataataTTGTTTCGTAATCATGTCGTCGCCATCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGttc                                <  2:277392/141‑1 (MQ=255)
                                                                                                               taataTTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGttct                               <  1:95406/141‑1 (MQ=255)
                                                                                                                aataTTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGttctt                              >  2:707669/1‑141 (MQ=255)
                                                                                                                      gTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTAc                        >  3:84012/1‑141 (MQ=255)
                                                                                                                            tAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTAc                  >  4:38752/1‑141 (MQ=255)
                                                                                                                              aTCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGc                >  2:423124/1‑141 (MQ=255)
                                                                                                                                cATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGCaa              >  4:22918/1‑141 (MQ=255)
                                                                                                                                  tGTCGTCGCCGTCGATTTCTGCATTAACTTCTTGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGCAAtt            <  1:778718/141‑1 (MQ=255)
                                                                                                                                  tGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGCAAtt            >  1:619414/1‑141 (MQ=255)
                                                                                                                                  tGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGCAAtt            >  1:619852/1‑141 (MQ=255)
                                                                                                                                   gtcgtcGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGCAATTc           <  3:193226/141‑1 (MQ=255)
                                                                                                                                   gtcgtcGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCACCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGCAATTc           >  1:962348/1‑141 (MQ=255)
                                                                                                                                    tcgtcgCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGCAATTcc          <  2:123340/141‑1 (MQ=255)
                                                                                                                                    tcgtcgCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGCAATTcc          <  3:56756/141‑1 (MQ=255)
                                                                                                                                     cgtcgCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGCAATTCCa         <  3:138931/141‑1 (MQ=255)
                                                                                                                                      gtcgCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGCAATTCCAt        >  1:286851/1‑141 (MQ=255)
                                                                                                                                        cgCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGCAATTCCATaa      >  2:564564/1‑141 (MQ=255)
                                                                                                                                           cGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATACTAATTTAGTACCATCATGATATTTCATAAATTGTTCTTTCTTACGTTTACGCAATTCCATAACAt   >  1:1032798/1‑141 (MQ=255)
                                                                                                                                            gTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAACTTAGTACCACCATGATCTTTCCTAAATTGTTCTTTATTACGTTTACGCAATTCCATAACAtt  >  1:1032924/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ACTGCTAAATTAGCAATTTCTGCTTCGATTTCTGCAAAATTCTTTTTATCACAATCTCTTACAAATGGTACTAATAATCCATCATCTGTAGAAACAGCTACACCAATATCATAATATTGTTTCGTAATCATGTCGTCGCCGTCGATTTCTGCATTAACTTCTGGATACTTTTTCAAAGCTGCTACAGAAGCTTTAGTAAAGAATGACATAAATCCTAATTTAGTACCATCATGATCTTTCATAAATTGTTCTTTCTTACGTTTACGCAATTCCATAACATT  >  USA300TCH1516_ALE/1450151‑1450431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑