Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,451,633 C→T 5.7% A763A (GCG→GCA odhA ← 2‑oxoglutarate dehydrogenase E1 component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,451,6330CT 5.7% 368.0 / 4.6 117A763A (GCG→GCAodhA2‑oxoglutarate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base C (47/62);  new base T (4/3);  total (51/66)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

GCTTGATATGATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCATATGCATGAGG  >  USA300TCH1516_ALE/1451493‑1451773
                                                                                                                                              |                                                                                                                                            
  ttGATATGATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCATTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGc                                                                                                                                            <  4:211607/141‑1 (MQ=255)
   tGATATGATTCTGTCAAAATTGGCGCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCt                                                                                                                                           <  1:46984/141‑1 (MQ=255)
      tattatTCTGTTAAAATTGGCTTAAATTTACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGTTGACATAACAACTAATGGTCTCATTTTTTCAGAATCTAAACTAGCCTCTtgt                                                                                                                                        <  4:350307/141‑1 (MQ=255)
      tatGATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTtgt                                                                                                                                        >  1:1016572/1‑141 (MQ=255)
       atGATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTtgtg                                                                                                                                       >  4:450079/1‑141 (MQ=255)
         gATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTTGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCa                                                                                                                                     >  4:319374/1‑141 (MQ=255)
         gATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCa                                                                                                                                     >  2:302790/1‑141 (MQ=255)
         gATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCa                                                                                                                                     <  2:852319/141‑1 (MQ=255)
             cTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCa                                                                                                                                 >  2:201331/1‑141 (MQ=255)
              tGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGTTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAAACTAAACTAGCCGCTTGTGCACGCaa                                                                                                                                <  2:164338/141‑1 (MQ=255)
               gTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAAt                                                                                                                               >  1:175152/1‑141 (MQ=255)
               gTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTGGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAAt                                                                                                                               <  4:178258/141‑1 (MQ=255)
               gTCAAAATTGGCTCAAATCCACCAGAAGTAAACTCATCAATTGGTTTGGCAACTGTTTTATTTCTCAATAAGCTTTTTTTTTTTTTTTCAACCAATGGTAGCATTTGTTCATTTTCTAAACTAGCTGCTTGTTAACGCAAt                                                                                                                               >  2:604220/1‑141 (MQ=255)
                tCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATa                                                                                                                              >  2:683492/1‑141 (MQ=255)
                tCAAAATTGGCTCAAATCCACCAGAAGTAAACTCATCAATTGGTTTGGCAACTGTTTTATTTCTCAATAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCTGCTTGTGCACGCAATa                                                                                                                              >  1:992103/1‑141 (MQ=255)
                    aaTTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTg                                                                                                                          <  3:393906/141‑1 (MQ=255)
                      tttgCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCAGTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGa                                                                                                                        <  2:255228/138‑1 (MQ=255)
                      ttGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGa                                                                                                                        <  1:265476/141‑1 (MQ=255)
                      ttGGCTCAAATCCACCAGAAGTAAACTCATCAATTGGTTTGGCAACTGTTTTATTTCTCAATAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCTGCTTGTGCACGCAATAAGTGGa                                                                                                                        <  1:944587/141‑1 (MQ=255)
                       tGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGaa                                                                                                                       <  1:421061/141‑1 (MQ=255)
                       tGGCTCAAATCCACCAGAAGTAAACTCATCAATTGGTTTGGCAACTGTTTTATTTCTCAATAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCTGCTTGTGCACGCAATAAGTGGaa                                                                                                                       >  1:17662/1‑141 (MQ=255)
                           tCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTCATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAaataa                                                                                                                   >  3:371784/1‑141 (MQ=255)
                            cAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAaataat                                                                                                                  <  2:231791/141‑1 (MQ=255)
                            cAAATCCACCAGAAGTAAATTCATCAATTGGTTNTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAaataat                                                                                                                  >  1:906574/1‑141 (MQ=255)
                            cAAATCCAACAGAAGTACACTAACCAATCGGTTTTGCAAGCGTTTTATTTCTCAGTAAGCTTTTTTGGTGACATCACAGCGAATGGTCGCATTTGTTTAGAATCTAACCTAGCCGCGTGTGCACGCAACAAGAGGAaataa                                                                                                                   >  2:1083049/1‑141 (MQ=255)
                             aaaTCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAAtt                                                                                                                 <  4:323472/141‑1 (MQ=255)
                              aaTCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTa                                                                                                                <  1:790344/141‑1 (MQ=255)
                                    ccaGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTTTTTTTTTTTCTACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAATTGGAACTAATTACTTGaa                                                                                                          >  4:6864/1‑141 (MQ=255)
                                      aGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACt                                                                                                        <  4:199320/141‑1 (MQ=255)
                                         aGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTTCACGCAATAAGTGGAGATAATTACTTGAACTAGa                                                                                                     >  1:294715/1‑141 (MQ=255)
                                         aGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGa                                                                                                     <  3:169468/141‑1 (MQ=255)
                                           tAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATa                                                                                                   <  1:573540/141‑1 (MQ=255)
                                             aaTTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAg                                                                                                 <  2:720596/141‑1 (MQ=255)
                                              aTTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGt                                                                                                >  1:1070122/1‑141 (MQ=255)
                                               ctcatcaATTGGTTTGGCAACTGTTTTATTTCTCAATAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCTGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGtt                                                                                               >  1:190634/2‑141 (MQ=255)
                                                         ggTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACtgtg                                                                                     <  2:396658/141‑1 (MQ=255)
                                                         ggTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACtgtg                                                                                     >  2:386958/1‑141 (MQ=255)
                                                         ggTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACtgtg                                                                                     <  1:912216/141‑1 (MQ=255)
                                                         ggTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACtgtg                                                                                     >  1:447805/1‑141 (MQ=255)
                                                          gTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGc                                                                                    <  1:167113/141‑1 (MQ=255)
                                                           ttttGCAACTGTTTTATTTCTCAGTAAGCTTTTTTTTTTCATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTATGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCa                                                                                   >  2:863466/1‑141 (MQ=255)
                                                           ttttGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCa                                                                                   <  2:481565/141‑1 (MQ=255)
                                                            tttGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCaa                                                                                  >  3:162935/1‑141 (MQ=255)
                                                             ttGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAat                                                                                 <  4:38224/141‑1 (MQ=255)
                                                             ttGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAat                                                                                 >  1:747472/1‑141 (MQ=255)
                                                             ttGCAACTGTTCTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAat                                                                                 <  1:766561/141‑1 (MQ=255)
                                                               gCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAatta                                                                               <  3:391826/141‑1 (MQ=255)
                                                               gCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGAAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAatta                                                                               <  3:343081/141‑1 (MQ=255)
                                                                    tGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTc                                                                          <  1:201331/141‑1 (MQ=255)
                                                                      ttttATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTcag                                                                        <  1:57522/141‑1 (MQ=255)
                                                                       tttATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTcagc                                                                       >  4:410405/1‑141 (MQ=255)
                                                                          aTTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCagcagc                                                                    >  4:379727/1‑141 (MQ=255)
                                                                          aTTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCagcagc                                                                    >  1:689680/1‑141 (MQ=255)
                                                                          aTTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCagcagc                                                                    <  1:951384/141‑1 (MQ=255)
                                                                             tctcAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTaa                                                                 >  4:200289/1‑141 (MQ=255)
                                                                             tctcAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTaa                                                                 >  4:74055/1‑141 (MQ=255)
                                                                               tcAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAAtt                                                               >  1:472660/1‑141 (MQ=255)
                                                                               tcAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAAtt                                                               >  1:550038/1‑141 (MQ=255)
                                                                                cAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTg                                                              <  1:190706/141‑1 (MQ=255)
                                                                                  gTAAGCTTTTTGGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGt                                                             <  3:329802/141‑1 (MQ=255)
                                                                                       cTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAAt                                                       >  1:228394/1‑141 (MQ=255)
                                                                                         tttttGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATc                                                      >  1:641227/1‑141 (MQ=255)
                                                                                         tttttGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATc                                                      >  3:349005/1‑141 (MQ=255)
                                                                                            ttGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGct                                                   <  1:857335/141‑1 (MQ=255)
                                                                                               ggTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGATAAATGTAAAAATCGctct                                                 <  2:396710/141‑1 (MQ=255)
                                                                                                gTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTa                                                >  3:62592/1‑141 (MQ=255)
                                                                                                   aCATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATc                                             >  1:515214/1‑141 (MQ=255)
                                                                                                   aCATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATc                                             <  1:712669/141‑1 (MQ=255)
                                                                                                      tAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTg                                          <  2:78194/141‑1 (MQ=255)
                                                                                                        acaacCAATGGTCGCATTTGTTCAGAATCTCAACTAGCCGCTCGCGCACGCCATACGTGGAAATACTTACTAGCACCAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTATTTGTAACACTTGCTCTAAtaataga                                        >  2:589145/1‑135 (MQ=255)
                                                                                                        acaacCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCt                                        >  1:794936/1‑141 (MQ=255)
                                                                                                         caacCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCtg                                       <  1:929174/141‑1 (MQ=255)
                                                                                                         caacCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCGCTAATCTTGCtg                                       >  1:266888/1‑141 (MQ=255)
                                                                                                          aacCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCtga                                      <  2:447805/141‑1 (MQ=255)
                                                                                                              aaTGGTCGCATTTGTTCAGAATCTAAACTAGCTGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGaa                                  <  2:992103/141‑1 (MQ=255)
                                                                                                              aaTGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGaa                                  <  1:302790/141‑1 (MQ=255)
                                                                                                                 ggTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGt                               >  2:412764/1‑141 (MQ=255)
                                                                                                                    cGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCa                            >  1:993363/1‑141 (MQ=255)
                                                                                                                    cGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCa                            >  1:74195/1‑141 (MQ=255)
                                                                                                                     gCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAg                           >  3:75852/1‑141 (MQ=255)
                                                                                                                      cATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAgg                          <  2:461330/141‑1 (MQ=255)
                                                                                                                      cATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAgg                          <  2:994569/141‑1 (MQ=255)
                                                                                                                       aTTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGc                         <  1:444946/141‑1 (MQ=255)
                                                                                                                           gTTCAGAATCTAAACTAGCTGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGTCCtt                     <  2:1074060/141‑1 (MQ=255)
                                                                                                                               aGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGAcc                 <  2:420469/141‑1 (MQ=255)
                                                                                                                               aGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGAcc                 <  1:155705/141‑1 (MQ=255)
                                                                                                                                 aaTCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCt               <  3:200289/141‑1 (MQ=255)
                                                                                                                                  aTCTAAACTAGCCGCTTGTGCACGCAATAAGTGGTAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTc              <  1:1007185/141‑1 (MQ=255)
                                                                                                                                    cTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCat            <  3:276022/141‑1 (MQ=255)
                                                                                                                                     tAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCatg           <  1:805227/141‑2 (MQ=255)
                                                                                                                                     tAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCata           >  2:109870/1‑141 (MQ=255)
                                                                                                                                     tAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCata           <  1:808220/141‑1 (MQ=255)
                                                                                                                                       aaCTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATg                                <  4:73090/118‑1 (MQ=255)
                                                                                                                                       aaCTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATg                                >  3:73090/1‑118 (MQ=255)
                                                                                                                                       aaCTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCatgca         <  3:184965/141‑4 (MQ=255)
                                                                                                                                        aCTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCATATGc        >  4:217612/1‑141 (MQ=255)
                                                                                                                                          tAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCATATGCAt      <  2:492443/141‑1 (MQ=255)
                                                                                                                                            gCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCATATGCATGa    <  2:544314/141‑1 (MQ=255)
                                                                                                                                              cGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCATATGCATGAgg  >  2:613849/1‑141 (MQ=255)
                                                                                                                                              cGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCATATGCATGAgg  >  3:105614/1‑141 (MQ=255)
                                                                                                                                              |                                                                                                                                            
GCTTGATATGATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCATATGCATGAGG  >  USA300TCH1516_ALE/1451493‑1451773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: