Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 99,276 G→C 5.8% intergenic (+70/+2) USA300HOU_RS00465 → / ← tetA_1 hypothetical protein/Tetracycline resistance protein, class C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE99,2760GC 5.8% 452.8 / 17.2 138intergenic (+70/+2)USA300HOU_RS00465/tetA_1hypothetical protein/Tetracycline resistance protein, class C
Reads supporting (aligned to +/- strand):  ref base G (67/63);  new base C (3/5);  total (70/68)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01

Only 100 of 139 total aligned reads displayed.

TCTAGACTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCTAAAAA  >  USA300TCH1516_ALE/99137‑99415
                                                                                                                                           |                                                                                                                                           
tCTAGACTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACgg                                                                                                                                            <  2:627599/141‑1 (MQ=255)
  tAGACTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTc                                                                                                                                          <  2:427375/141‑1 (MQ=255)
   aGACTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCa                                                                                                                                         >  2:1022250/1‑141 (MQ=255)
   aGACTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCa                                                                                                                                         >  3:198645/1‑141 (MQ=255)
     aCTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCAct                                                                                                                                       <  4:66411/141‑1 (MQ=255)
     aCTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCAct                                                                                                                                       <  1:223847/141‑1 (MQ=255)
     aCTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCAct                                                                                                                                       <  4:113310/141‑1 (MQ=255)
     aCTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGCTAAAGAAGAGTGACGGTCAct                                                                                                                                       <  2:308479/141‑1 (MQ=255)
      cTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCActc                                                                                                                                      >  1:942052/1‑141 (MQ=255)
         tACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACtcttc                                                                                                                                   <  4:35946/141‑1 (MQ=255)
         tACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTCACtcttc                                                                                                                                   <  3:192376/141‑1 (MQ=255)
           ccGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTcttctt                                                                                                                                 >  2:803361/1‑141 (MQ=255)
           ccGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTcttctt                                                                                                                                 >  2:144849/1‑141 (MQ=255)
           ccGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTcttctt                                                                                                                                 >  3:34898/1‑141 (MQ=255)
                  aacaaATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGc                                                                                                                          <  2:12754/141‑1 (MQ=255)
                       aTCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGTCGGTCACTCTTCTTTATGTGCAtatt                                                                                                                     <  1:796212/141‑1 (MQ=255)
                       aTCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCAtatt                                                                                                                     <  1:443891/141‑1 (MQ=255)
                       aTCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCAtatt                                                                                                                     >  4:444703/1‑141 (MQ=255)
                            gcTGATGTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGGGAAGTGTGGGTGCAGAGAGAACTATGCCCATCGATAAAGGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCAtattttatt                                                                                                                <  2:256540/140‑1 (MQ=255)
                            aCTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCAtattttatt                                                                                                                >  2:464435/1‑141 (MQ=255)
                             cTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCAtattttattt                                                                                                               <  3:105824/141‑1 (MQ=255)
                             cTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCAtattttattt                                                                                                               <  2:908462/141‑1 (MQ=255)
                             cTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCAtattttattt                                                                                                               >  3:62157/1‑141 (MQ=255)
                              tGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATattttatttt                                                                                                              <  1:537040/141‑1 (MQ=255)
                              tGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGACAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATattttatttt                                                                                                              <  2:573232/141‑1 (MQ=255)
                               gATTTATTTAAGACCATAAAAAATGATTTGCAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCAATCGATAAATGGTCGCTTGTTAACGAAGAGTGACGGTCACTCTTATTTCTGTGAATATTTTATTTTg                                                                                                             >  2:514449/1‑141 (MQ=255)
                               gATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTg                                                                                                             <  3:448872/141‑1 (MQ=255)
                                atttatttAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGc                                                                                                            >  4:391915/1‑141 (MQ=255)
                                atttatttAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGc                                                                                                            >  2:160047/1‑141 (MQ=255)
                                atttattgGAGACCATAAATAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACGGAGCCCGTCGATAAATGGTCGCTTGTTAAAGAAGAGTGTCGGTCATTCTTCTTTATGTGCATATTTTATTTTGc                                                                                                            <  2:130422/141‑1 (MQ=255)
                                  ttatttaAGACCATAAAAAATGATTTGAAACAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCt                                                                                                          <  4:184742/141‑1 (MQ=255)
                                  ttatttaAGACCATAAAAAATGATTTGAAAAAGTGGAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCt                                                                                                          <  4:315506/141‑1 (MQ=255)
                                    agttaAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGt                                                                                                        <  4:303281/139‑1 (MQ=255)
                                     tttaAGACCGGAAAAACTGATTTGCGCAAGCGAAGAAGTGACGGGTGGGGGCAGTGAGAACTAAGCCGATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTGGCCTGtt                                                                                                       >  4:281183/1‑141 (MQ=255)
                                     tttaAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGtt                                                                                                       >  2:789224/1‑141 (MQ=255)
                                     tttaAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGCCACTCTTCTTTATGTGCATATTTTATTTTGCCTGtt                                                                                                       >  1:985747/1‑141 (MQ=255)
                                        aaGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTg                                                                                                    <  1:728599/141‑1 (MQ=255)
                                         aGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGt                                                                                                   >  3:257877/1‑141 (MQ=255)
                                            ccATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTaa                                                                                                <  1:868235/141‑1 (MQ=255)
                                             cATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAAc                                                                                               >  3:106656/1‑141 (MQ=255)
                                               tAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACaa                                                                                             <  1:996789/141‑1 (MQ=255)
                                                  aaaaTGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAagca                                                                                          <  3:211648/141‑1 (MQ=255)
                                                  aaaaTGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAagca                                                                                          >  1:971725/1‑141 (MQ=255)
                                                    aaTGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAagcagc                                                                                        >  3:294863/1‑141 (MQ=255)
                                                     aTGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAgcagca                                                                                       >  2:687137/1‑141 (MQ=255)
                                                      tGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGcagcag                                                                                      <  2:741811/141‑1 (MQ=255)
                                                       gATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAgt                                                                                     >  3:168671/1‑141 (MQ=255)
                                                        aTTTGAAAAAGTGGAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAgtg                                                                                    >  2:963868/1‑141 (MQ=255)
                                                        aTTTGAAAAAGTGAAGTAGAGAAGTGTGGGTGCAGTGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAgtt                                                                                    >  2:336845/1‑140 (MQ=255)
                                                         tttGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAgtgt                                                                                   <  1:167064/141‑1 (MQ=255)
                                                         tttGAAAAAGTGAAGTAGTGAAGTGCGGGTGCAGAGAGAAATACGCCCAAACATCAAACCTAAACAGTTAACGAAGTGTGTCGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAgtgt                                                                                   <  3:42342/141‑1 (MQ=255)
                                                           tGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTaa                                                                                 <  2:249462/141‑1 (MQ=255)
                                                           tGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTaa                                                                                 <  1:455924/141‑1 (MQ=255)
                                                               aaaGTGAAGTAGGGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACaaa                                                                             <  2:649254/141‑1 (MQ=255)
                                                                  gtgaagtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAatat                                                                          >  3:327829/1‑141 (MQ=255)
                                                                  gtgaagtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAatat                                                                          >  1:1065593/1‑141 (MQ=255)
                                                                  gtgaagtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAatat                                                                          >  1:541307/1‑141 (MQ=255)
                                                                   tgaagtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTGTGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATg                                                                         >  2:862567/1‑141 (MQ=255)
                                                                       gtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTa                                                                     <  1:407527/141‑1 (MQ=255)
                                                                       gtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTa                                                                     >  4:203879/1‑141 (MQ=255)
                                                                       gtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTa                                                                     <  2:893917/141‑1 (MQ=255)
                                                                       gtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTa                                                                     <  2:545006/141‑1 (MQ=255)
                                                                       gtagtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTAAACAAGCAGCAGTGTAACAAATATGAGTa                                                                     <  2:231689/141‑1 (MQ=255)
                                                                          gtgaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAgg                                                                  <  1:90378/141‑1 (MQ=255)
                                                                          gtgaagtGTCGGTGCAGAGAGAACTCAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTCATGTGCATATTTAATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAgg                                                                  <  2:839827/141‑1 (MQ=255)
                                                                            gaagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAAAGTGACGGTCAACCTTCTTTATGTGCATATTGTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACGAATATGAGTAAGGAt                                                                >  4:406094/1‑141 (MQ=255)
                                                                             aagtGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATa                                                               >  2:405926/1‑141 (MQ=255)
                                                                                      tGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGta                                                      >  1:121760/1‑141 (MQ=255)
                                                                                         agagagAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTataa                                                   <  1:64468/141‑1 (MQ=255)
                                                                                                aCTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTc                                                                                                                                          <  2:29847/47‑1 (MQ=255)
                                                                                                aCTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACCGTc                                                                                                                                          >  1:29847/1‑47 (MQ=255)
                                                                                                  tAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCAGTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGACAAAATGAGTATAATATAGAAAc                                          >  3:378662/1‑141 (MQ=255)
                                                                                                          tCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTa                                  >  1:607657/1‑141 (MQ=255)
                                                                                                           cGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCCCTCTGCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATAAAGAAACCGAATTTAt                                 >  4:381313/1‑141 (MQ=255)
                                                                                                            gATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATc                                <  4:156250/141‑1 (MQ=255)
                                                                                                            gATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATc                                >  1:685545/1‑141 (MQ=255)
                                                                                                               aaaTGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCAtt                             >  1:677234/1‑141 (MQ=255)
                                                                                                                  tGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAAt                          >  2:857783/1‑141 (MQ=255)
                                                                                                                  tGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAAt                          >  1:90624/1‑141 (MQ=255)
                                                                                                                  tGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAAt                          >  1:97412/1‑141 (MQ=255)
                                                                                                                   ggTCGCTTGTTAAAGAAGAGTGACGGTCACTCATCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAAtt                         <  3:407335/141‑1 (MQ=255)
                                                                                                                      cGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCa                      <  3:444703/141‑1 (MQ=255)
                                                                                                                       gCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCAt                     <  3:329582/141‑1 (MQ=255)
                                                                                                                          tGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTaa                  <  2:835995/141‑1 (MQ=255)
                                                                                                                           gTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAAt                 <  1:42679/141‑1 (MQ=255)
                                                                                                                             tAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATcc               >  4:239411/1‑141 (MQ=255)
                                                                                                                                agaagaGTGACGGTCACTCTTCTTTATGTGCATATTTTATATTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATTCATc            >  3:146703/1‑141 (MQ=255)
                                                                                                                                    gagTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTct        >  1:533980/1‑140 (MQ=255)
                                                                                                                                    gagTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTcc        >  4:78595/1‑141 (MQ=255)
                                                                                                                                    gagTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTcc        >  1:389934/1‑141 (MQ=255)
                                                                                                                                    gagTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTcc        >  1:1085763/1‑141 (MQ=255)
                                                                                                                                    gagTGACCGTCACTCTTCTTTATGTGCATATTTTATTTTGCTTGTTTTGTTACCAAGCAGCAGTGTAATAAATATGTGTAAGGAAAAAAAGAGTATAATATAGAAACCGAATTTATCATCAATTTCATTAACACATCttac        >  4:141775/1‑139 (MQ=255)
                                                                                                                                     agTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCt       <  3:230678/141‑1 (MQ=255)
                                                                                                                                     agTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCt       <  1:1077007/141‑1 (MQ=255)
                                                                                                                                     agTGACCGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCt       <  4:220552/141‑1 (MQ=255)
                                                                                                                                      gTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCTa      <  2:380782/141‑1 (MQ=255)
                                                                                                                                      gTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCTa      >  1:303674/1‑141 (MQ=255)
                                                                                                                                        gACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCTaaa    >  2:876837/1‑141 (MQ=255)
                                                                                                                                        gACGGTCACTCTTCTTNATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTCTCATTAATTTCCTTAATCCATCTTCCTaaa    >  1:863798/1‑141 (MQ=255)
                                                                                                                                          cGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCTaaaaa  <  3:419975/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TCTAGACTTTACCGAAGCAACAAATCTCACTGATTTATTTAAGACCATAAAAAATGATTTGAAAAAGTGAAGTAGTGAAGTGTGGGTGCAGAGAGAACTAAGCCCATCGATAAATGGTCGCTTGTTAAAGAAGAGTGACGGTCACTCTTCTTTATGTGCATATTTTATTTTGCCTGTTTTGTTAACAAGCAGCAGTGTAACAAATATGAGTAAGGATAAAATGAGTATAATATAGAAACCGAATTTATCATTAATTTCATTAATCCATCTTCCTAAAAA  >  USA300TCH1516_ALE/99137‑99415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: