Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,503,111 A→G 5.8% I234I (ATT→ATC norB_4 ← Quinolone resistance protein NorB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,503,1110AG 5.8% 342.7 / 7.2 115I234I (ATT→ATCnorB_4Quinolone resistance protein NorB
Reads supporting (aligned to +/- strand):  ref base A (48/59);  new base G (4/3);  total (53/62)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

ATTTAACAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTCAGGTGT  >  USA300TCH1516_ALE/1502973‑1503251
                                                                                                                                           |                                                                                                                                             
aTTTAACAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGtaat                                                                                                                                             <  1:679390/141‑1 (MQ=255)
   tAACAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATaaa                                                                                                                                         <  2:45279/141‑1 (MQ=255)
   tAACAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATaaa                                                                                                                                         <  4:354146/141‑1 (MQ=255)
    aaCAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATaaaa                                                                                                                                        >  2:547207/1‑141 (MQ=255)
     aCAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATaaaaa                                                                                                                                       <  1:896370/141‑1 (MQ=255)
     aCAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATaaaaa                                                                                                                                       <  3:437494/141‑1 (MQ=255)
      cAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAaag                                                                                                                                      <  1:334564/141‑1 (MQ=255)
        aaaaGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAaagaa                                                                                                                                    >  1:366249/1‑141 (MQ=255)
          aaGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAACAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGt                                                                                                                                  <  2:935301/141‑1 (MQ=255)
          aaGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGt                                                                                                                                  <  1:4606/141‑1 (MQ=255)
           aGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTg                                                                                                                                 >  1:1015964/1‑141 (MQ=255)
              ttGAAGCTGTTGCACCTGTGTACGCTTTATTTTTAACTAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAgg                                                                                                                              >  1:994288/1‑141 (MQ=255)
              ttGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCATGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAgg                                                                                                                              >  2:365353/1‑141 (MQ=255)
                gAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTCTAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGtt                                                                                                                            >  1:762151/1‑141 (MQ=255)
                gAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGtt                                                                                                                            >  4:377713/1‑141 (MQ=255)
                gAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGtt                                                                                                                            >  1:536078/1‑141 (MQ=255)
                gAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGtt                                                                                                                            >  4:338224/1‑141 (MQ=255)
                gAAGCTGTTGCACCTGTATAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAGCTATAAATAAACTAAAAGATCCAATTGCAATTGCTAATAAAGTGATAAAAAGAAGTGAGGtt                                                                                                                            >  2:571825/1‑141 (MQ=255)
                     tGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACAcc                                                                                                                       <  2:628125/141‑1 (MQ=255)
                     tGTTGAACCTGTGTAAGCTATATTTTTAAATAATTTAACCTAGATACAAAGCTTCATAAAACGCTTTTCAAGAACTATAAATAAACTACACGAACAAATTCAAATAGATAATAAAGTCATAAAAAGAAGTGAggatccaac                                                                                                                       >  2:48099/1‑134 (MQ=255)
                         gCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGCTTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAAt                                                                                                                   >  2:84332/1‑141 (MQ=255)
                          cACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAAtt                                                                                                                  >  1:966153/1‑141 (MQ=255)
                          aaCCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAAtt                                                                                                                  <  2:456981/140‑1 (MQ=255)
                           aCCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTc                                                                                                                 >  4:450013/1‑141 (MQ=255)
                            ccTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCt                                                                                                                <  2:752612/141‑1 (MQ=255)
                            ccTGTGTAAGCTTTATATTTAAATAATTTAAAATAGATTAAAGGATTTGAAGCACGAAAATCAGAAAATATAAATAAAATAAAAGAACCAAATGCAAAAGCTAATAAAGTAATAAAAAGAAGGAAGGTTATACATAATTCt                                                                                                                <  4:353689/141‑1 (MQ=255)
                             cTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTg                                                                                                               >  1:489811/1‑141 (MQ=255)
                                tgtAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTCGCACGCTTTTCACGATCTATCCATTAACAATTAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATc                                                                                                            <  3:386470/141‑1 (MQ=255)
                                tgtAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATc                                                                                                            >  1:430003/1‑141 (MQ=255)
                                tgtAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATc                                                                                                            <  3:125681/141‑1 (MQ=255)
                                     gCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATTGCTAATAAAGTGATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTa                                                                                                       <  1:258062/141‑1 (MQ=255)
                                      cTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAg                                                                                                      >  2:464862/1‑141 (MQ=255)
                                         tATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTaa                                                                                                   >  1:384620/1‑141 (MQ=255)
                                           tttttAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAAt                                                                                                  <  4:453968/141‑1 (MQ=255)
                                           tttttAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAAtt                                                                                                 <  3:414712/141‑1 (MQ=255)
                                             tttAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTaa                                                                                               >  2:276509/1‑141 (MQ=255)
                                               tAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATATAAAGAAGTGAGGTTACAACTAATTTTGATACTTTAGTAATTaaaa                                                                                             >  2:93385/1‑141 (MQ=255)
                                                aaaTAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAat                                                                                            >  2:784663/1‑141 (MQ=255)
                                                aaaTAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAat                                                                                            >  1:146024/1‑141 (MQ=255)
                                                    aatTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATAttt                                                                                        <  1:357440/141‑1 (MQ=255)
                                                     atTTAGGATCGATTAAAGGATTTGTAGCGCTCTCTTCAAGAACTATAAATAAACTAAAATATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTa                                                                                       <  3:202524/141‑1 (MQ=255)
                                                      tttAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATAAAAATATTTaa                                                                                      >  4:445990/1‑141 (MQ=255)
                                                       ttAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGCCCCTTGAGCAAGTAAAATATTTaaa                                                                                     <  2:1087204/141‑1 (MQ=255)
                                                       ttAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTaaa                                                                                     <  1:987687/141‑1 (MQ=255)
                                                            aTCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTgag                                                                                <  1:922957/141‑1 (MQ=255)
                                                               gATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTgaggag                                                                             >  1:851254/1‑141 (MQ=255)
                                                               gATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTgaggag                                                                             <  4:13332/141‑1 (MQ=255)
                                                                aTTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGc                                                                            >  2:1058614/1‑141 (MQ=255)
                                                                aTTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGc                                                                            <  1:178730/141‑1 (MQ=255)
                                                                     aGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATaa                                                                        <  1:259768/141‑1 (MQ=255)
                                                                       gATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGa                                                                     <  2:159974/141‑1 (MQ=255)
                                                                       gATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGa                                                                     <  2:1045051/141‑1 (MQ=255)
                                                                       gATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGa                                                                     <  4:212057/141‑1 (MQ=255)
                                                                        aTTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGAc                                                                    <  2:384753/141‑1 (MQ=255)
                                                                         tttGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACt                                                                   <  3:228979/141‑1 (MQ=255)
                                                                              aGCACGCATTTAAAGAACTAAAAATAAAATAAAAGAAACAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATAAAAAAAAAAAAAAAATATTTAAACTGAGGAGCATAATGACTAAAAg                                                              <  4:227493/141‑1 (MQ=255)
                                                                               gCACGCTTTTCAAGAACTATAAAGAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATGCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGa                                                             <  1:49192/141‑1 (MQ=255)
                                                                                  cGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAAcc                                                          <  2:62617/141‑1 (MQ=255)
                                                                                   gCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCa                                                         <  2:820740/141‑1 (MQ=255)
                                                                                   gCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCa                                                         >  4:26856/1‑141 (MQ=255)
                                                                                   gCTTTTCAAGAACTATAAATAAACTAAAAGATCCAAGTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAACCTGAGGAGCATAATGACTAAAAGAACCa                                                         <  2:820599/141‑1 (MQ=255)
                                                                                    cTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAg                                                        <  1:206900/141‑1 (MQ=255)
                                                                                        tCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTTAGGAGCATAATGACTAAAAGAACCAGACCt                                                    >  1:1017476/1‑141 (MQ=255)
                                                                                        tCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCt                                                    >  1:627521/1‑141 (MQ=255)
                                                                                         cAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCtt                                                   >  2:51734/1‑141 (MQ=255)
                                                                                          aaGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCttt                                                  >  1:706461/1‑141 (MQ=255)
                                                                                          aaGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCttt                                                  <  1:996026/141‑1 (MQ=255)
                                                                                           aGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTa                                                 >  1:672172/1‑141 (MQ=255)
                                                                                              aCTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATg                                              >  2:524771/1‑141 (MQ=255)
                                                                                              aCTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATg                                              >  1:450000/1‑141 (MQ=255)
                                                                                               ttttaaataaaCTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGt                                             >  4:170317/4‑141 (MQ=255)
                                                                                               cTATAAAGAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGt                                             <  2:600263/141‑1 (MQ=255)
                                                                                                 ataaataaACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTTAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCa                                           >  4:322723/1‑141 (MQ=255)
                                                                                                   aaataaaCTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCaaa                                         >  4:162474/1‑141 (MQ=255)
                                                                                                    aataaaCAAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAat                                        <  2:861815/141‑1 (MQ=255)
                                                                                                     ataaaCTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAatt                                       <  4:333565/141‑1 (MQ=255)
                                                                                                        aaCTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAatttat                                    >  2:246247/1‑141 (MQ=255)
                                                                                                         aCTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAatttatt                                   <  2:491880/141‑1 (MQ=255)
                                                                                                          cTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAatttattt                                  >  2:533312/1‑141 (MQ=255)
                                                                                                           tAAAAGATCCAATTGCAATTGCTAATAAAGTGATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAAGTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAAtttattta                                 >  2:255155/1‑141 (MQ=255)
                                                                                                           tAAAAGATCCAATTGCAATAGCTAATAAAGTAAGAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGATCCAGACCTGTAATGTCAAAtttattta                                 <  2:929235/141‑1 (MQ=255)
                                                                                                            aaaaaGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAAtttattta                                 <  2:797713/140‑1 (MQ=255)
                                                                                                              aaaGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTaga                               <  1:788129/141‑1 (MQ=255)
                                                                                                                  aTCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGaaa                           <  1:270338/141‑1 (MQ=255)
                                                                                                                     cAATTGCAAGAGCTATTAAATTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCg                        >  2:222334/1‑141 (MQ=255)
                                                                                                                      aaTTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCga                       <  1:37153/141‑1 (MQ=255)
                                                                                                                        ttGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgatt                     <  1:388136/141‑1 (MQ=255)
                                                                                                                          gCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgattta                   >  2:163772/1‑141 (MQ=255)
                                                                                                                           cAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgatttag                  >  3:299014/1‑141 (MQ=255)
                                                                                                                              ttgCTAATAAAGTGATAAAAAGAAGTGAGGTTTCACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAAGTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgatttagatt               <  1:571825/139‑1 (MQ=255)
                                                                                                                              ttgCTAATAAAGTGATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAAGTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgatttagatt               <  2:573485/139‑1 (MQ=255)
                                                                                                                              tAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgatttagatt               <  1:11819/141‑1 (MQ=255)
                                                                                                                              tAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgatttagatt               <  1:11769/141‑1 (MQ=255)
                                                                                                                                     taaAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTc        >  1:688315/1‑141 (MQ=255)
                                                                                                                                     taaAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTc        <  2:326155/141‑1 (MQ=255)
                                                                                                                                      aaaGTGATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAAGTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTCa       >  2:1059111/1‑141 (MQ=255)
                                                                                                                                        aGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTCAgg     <  1:985818/141‑1 (MQ=255)
                                                                                                                                          taataaAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTCAGgtg   >  1:433785/1‑141 (MQ=255)
                                                                                                                                          taataaAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACAAGACCTATAATGTCAAATTTCTTACAGGAAAAAGAGTTAGGTTTATTTTCAtgtg   >  3:198938/1‑141 (MQ=255)
                                                                                                                                           aataaAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTCAGgtgt  >  2:327671/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                             
ATTTAACAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTCAGGTGT  >  USA300TCH1516_ALE/1502973‑1503251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: