Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,504,143 T→C 5.9% E383E (GAA→GAG steT ← Serine/threonine exchanger SteT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,504,1430TC 5.9% 338.2 / 9.0 119E383E (GAA→GAGsteTSerine/threonine exchanger SteT
Reads supporting (aligned to +/- strand):  ref base T (62/49);  new base C (4/3);  total (66/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 122 total aligned reads displayed.

ACTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTT  >  USA300TCH1516_ALE/1504003‑1504282
                                                                                                                                            |                                                                                                                                            
aCTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGt                                                                                                                                              <  1:229978/141‑1 (MQ=255)
   ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                           >  1:650753/1‑141 (MQ=255)
   ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                           >  1:1087433/1‑141 (MQ=255)
   ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                           >  1:192132/1‑141 (MQ=255)
   ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                           >  1:259384/1‑141 (MQ=255)
   ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                           >  1:408223/1‑141 (MQ=255)
     tataCCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCat                                                                                                                                         >  2:662759/1‑141 (MQ=255)
       taCCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCatat                                                                                                                                       <  2:544815/141‑1 (MQ=255)
         ccAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATAttt                                                                                                                                     >  2:450130/1‑141 (MQ=255)
         ccAAGTGCTGCTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATAttt                                                                                                                                     >  1:367734/1‑141 (MQ=255)
           aaGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTgg                                                                                                                                   >  2:678084/1‑141 (MQ=255)
            aGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGt                                                                                                                                  >  1:793945/1‑141 (MQ=255)
             gTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGtt                                                                                                                                 <  3:320321/141‑1 (MQ=255)
             gTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGtt                                                                                                                                 >  1:44913/1‑141 (MQ=255)
             gTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGtt                                                                                                                                 <  1:329296/141‑1 (MQ=255)
                 tGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACg                                                                                                                             >  4:164144/1‑141 (MQ=255)
                  gTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGt                                                                                                                            <  2:916121/141‑1 (MQ=255)
                  gTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATCAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGt                                                                                                                            >  4:123162/1‑141 (MQ=255)
                   ttattaGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGtt                                                                                                                           <  1:41295/141‑1 (MQ=255)
                   ttattaGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGtt                                                                                                                           <  1:638362/141‑1 (MQ=255)
                        aGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCtt                                                                                                                      >  2:226188/1‑141 (MQ=255)
                          aaTTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTaa                                                                                                                    >  3:199976/1‑141 (MQ=255)
                          aaTTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATCAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGTTTTCTTaa                                                                                                                    >  1:1050395/1‑141 (MQ=255)
                          aaTTCCAATGATTGATAATATAAAATGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAACGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTaa                                                                                                                    <  4:411540/141‑1 (MQ=255)
                           aTTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCCGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTaaa                                                                                                                   >  1:461967/1‑141 (MQ=255)
                             tCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAat                                                                                                                 >  3:406652/1‑141 (MQ=255)
                               cAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAatta                                                                                                               <  1:124976/141‑1 (MQ=255)
                               cAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAatta                                                                                                               >  2:651244/1‑141 (MQ=255)
                               cAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCACATTTGGTTCACGTTTTCTTAAAatta                                                                                                               >  2:386183/1‑141 (MQ=255)
                                   gATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTAc                                                                                                           >  1:507770/1‑141 (MQ=255)
                                    aTTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTAcc                                                                                                          <  1:1082750/141‑1 (MQ=255)
                                      tGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGc                                                                                                        <  1:51982/141‑1 (MQ=255)
                                      tGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGc                                                                                                        >  1:751886/1‑141 (MQ=255)
                                         tAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGcaac                                                                                                     >  2:681587/1‑141 (MQ=255)
                                          aaTATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGcaaca                                                                                                    <  1:724588/141‑1 (MQ=255)
                                            tataAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACaaa                                                                                                  <  2:44913/141‑1 (MQ=255)
                                             ataAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAAt                                                                                                 <  3:373424/141‑1 (MQ=255)
                                              taAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATg                                                                                                <  4:364818/141‑1 (MQ=255)
                                               aaaTTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACCAATGa                                                                                               <  4:116096/141‑1 (MQ=255)
                                                aaTTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGAc                                                                                              <  3:356365/141‑1 (MQ=255)
                                                aaTTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGAc                                                                                              >  2:566191/1‑141 (MQ=255)
                                                aaTTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGAc                                                                                              >  2:393951/1‑141 (MQ=255)
                                                  ttGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACAt                                                                                            <  2:550179/141‑1 (MQ=255)
                                                      ttAAACAGTGTATTAATTAATACAAATGATACTGCCAAAATAGAAATTAAAGGTATGAACGGATATAAAGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACaa                                                                                        <  4:429788/140‑1 (MQ=255)
                                                      gtAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGGCATACaa                                                                                        <  4:143643/141‑1 (MQ=255)
                                                       tAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAAt                                                                                       >  2:190905/1‑141 (MQ=255)
                                                       tAAACAGTGTATTAATCAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGTTTTCTTAAAATTATTACAGCAACAAATGACATACAAt                                                                                       <  4:113951/141‑1 (MQ=255)
                                                        aaaCAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATa                                                                                      >  4:77405/1‑141 (MQ=255)
                                                         aaCAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAg                                                                                     >  2:452120/1‑141 (MQ=255)
                                                         aaCAGTGTATTAATTAATACAAATGATCCTGCAAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGGTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATac                                                                                     <  2:835752/141‑2 (MQ=255)
                                                          aCAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGa                                                                                    <  4:365310/141‑1 (MQ=255)
                                                             gtgtATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGaaca                                                                                 <  2:952006/141‑1 (MQ=255)
                                                              tgtATTAATTAAAACGAATGGTCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGaacaa                                                                                >  4:204531/1‑141 (MQ=255)
                                                              tgtATTAATCAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGTTTTCTTAAAATTATTACAGCAACAAATGACATACAATAGaacaa                                                                                >  1:367876/1‑141 (MQ=255)
                                                                 attaattaATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTCCTTTATATGGTCGTACAATATTTGGTTCACGATTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCa                                                                             <  3:388569/141‑1 (MQ=255)
                                                                 attaattaATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCa                                                                             >  2:241012/1‑141 (MQ=255)
                                                                  ttaattaaTACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCaa                                                                            >  2:145591/1‑141 (MQ=255)
                                                                  ttaattaaTACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCaa                                                                            <  1:554321/141‑1 (MQ=255)
                                                                  ttaattaaTACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCaa                                                                            <  3:361371/141‑1 (MQ=255)
                                                                  ttaattaaTACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCaa                                                                            >  1:415225/1‑141 (MQ=255)
                                                                      ttaatACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATaa                                                                        <  2:554027/141‑1 (MQ=255)
                                                                          tACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACaaa                                                                    >  2:285653/1‑141 (MQ=255)
                                                                           aCAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAg                                                                   >  4:124308/1‑141 (MQ=255)
                                                                              aaTGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGAtt                                                                <  3:440193/141‑1 (MQ=255)
                                                                              aaTGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGAtt                                                                <  1:815184/141‑1 (MQ=255)
                                                                               aTGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTa                                                               >  1:318215/1‑141 (MQ=255)
                                                                                tGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTaa                                                              >  1:819962/1‑141 (MQ=255)
                                                                                  aTCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACa                                                            <  1:811994/141‑1 (MQ=255)
                                                                                  aTCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGtt                                                                                                                           <  2:755239/78‑1 (MQ=255)
                                                                                  aTCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGtt                                                                                                                           >  1:755239/1‑78 (MQ=255)
                                                                                    ccTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACata                                                          <  2:872088/141‑1 (MQ=255)
                                                                                    ccTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACata                                                          <  4:372941/141‑1 (MQ=255)
                                                                                     cTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACatat                                                         >  1:534391/1‑141 (MQ=255)
                                                                                     cTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACatat                                                         >  1:998499/1‑141 (MQ=255)
                                                                                            aaTAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTAGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTATAAt                                                  >  4:416071/1‑141 (MQ=255)
                                                                                                gCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCTCATCAATTTACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTa                                              >  3:87598/1‑141 (MQ=255)
                                                                                                gCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTa                                              >  2:1031004/1‑141 (MQ=255)
                                                                                                gCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTa                                              >  2:946546/1‑141 (MQ=255)
                                                                                                gCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTa                                              >  4:244894/1‑141 (MQ=255)
                                                                                                  aaTTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATc                                            <  3:89416/141‑1 (MQ=255)
                                                                                                            aTGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTccc                                 <  3:189423/141‑1 (MQ=255)
                                                                                                            aTGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTccc                                 <  1:199642/141‑1 (MQ=255)
                                                                                                               aTCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCAtt                              <  2:609532/141‑1 (MQ=255)
                                                                                                                 cGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGa                            >  2:346584/1‑141 (MQ=255)
                                                                                                                   gATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGAca                          >  2:846250/1‑141 (MQ=255)
                                                                                                                   gATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGAca                          >  3:341654/1‑141 (MQ=255)
                                                                                                                     tataACGGTACTTTATATGGACGCTCCATATTTGGTTCACGTTTTCTTAAAATTATTACAGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATGTTTGTTATTGTATCAAATGCTCCCATTGAcatc                        <  2:952616/141‑1 (MQ=255)
                                                                                                                         aCGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCatga                    <  1:205366/141‑1 (MQ=255)
                                                                                                                         aCGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCatga                    >  3:192452/1‑141 (MQ=255)
                                                                                                                         aCGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCatga                    >  1:1012104/1‑141 (MQ=255)
                                                                                                                          cGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCatgat                   <  4:398433/141‑1 (MQ=255)
                                                                                                                          cGGTACTCTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAATGCGCCCCATTGACATCatgat                   <  2:1087433/141‑1 (MQ=255)
                                                                                                                                 ttATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCg            >  3:427120/1‑141 (MQ=255)
                                                                                                                                  tataTGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGa           <  2:456386/141‑1 (MQ=255)
                                                                                                                                  tataTGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGa           <  2:408223/141‑1 (MQ=255)
                                                                                                                                  tataTGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGa           <  2:192132/141‑1 (MQ=255)
                                                                                                                                     atGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCTTGATGATAGCGATta        <  2:923411/141‑1 (MQ=255)
                                                                                                                                     atGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATta        <  3:150668/141‑1 (MQ=255)
                                                                                                                                         tCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCCCCCATTGACATCATGATGATAGCGATTATAAg    >  2:1053772/1‑141 (MQ=255)
                                                                                                                                           gTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGtt  >  2:881445/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ACTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTT  >  USA300TCH1516_ALE/1504003‑1504282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: