BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,517,372	C→A	5.9%	D132Y (GAT→TAT)	USA300HOU_RS07405 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,517,372	0	C	A	5.9%	421.1 / 7.6	127	D132Y (GAT→TAT)	USA300HOU_RS07405	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (56/63); new base A (2/6); total (58/69)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

ACCCATTCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTA  >  USA300TCH1516_ALE/1517232‑1517509

tcccATTCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTAGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATc                                                                                                                                           <  2:517675/140‑1 (MQ=255)

acCCATTCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATc                                                                                                                                           <  2:819001/141‑1 (MQ=255)

   cATTCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGt                                                                                                                                        <  1:985945/141‑1 (MQ=255)

    aTTCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTa                                                                                                                                       >  1:1013531/1‑141 (MQ=255)

      tCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTAcc                                                                                                                                     <  1:751211/141‑1 (MQ=255)

      tCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTAcc                                                                                                                                     <  1:996839/141‑1 (MQ=255)

       cTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTAccc                                                                                                                                    >  4:214408/1‑141 (MQ=255)

       cTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTAccc                                                                                                                                    >  2:1042631/1‑141 (MQ=255)

         aaTCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCctc                                                                                                                                  <  3:355423/141‑1 (MQ=255)

         aaTCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCctc                                                                                                                                  <  2:993249/141‑1 (MQ=255)

         aaTCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCctc                                                                                                                                  >  2:515201/1‑141 (MQ=255)

         aaTCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCctc                                                                                                                                  <  2:158524/141‑1 (MQ=255)

          aTCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCctct                                                                                                                                 >  2:494186/1‑141 (MQ=255)

           tCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTTACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCtt                                                                                                                                >  1:803765/1‑141 (MQ=255)

           tCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCtt                                                                                                                                >  1:925349/1‑141 (MQ=255)

           tCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATAAGTACCCTCtt                                                                                                                                <  2:655347/141‑1 (MQ=255)

           tCCTTCATCCAACAGTCGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATAAGTAcc                                                                                                                                     >  2:325526/1‑136 (MQ=255)

           tCCTTCATCCAACAGTCGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATAAGTAcc                                                                                                                                     <  1:325526/136‑1 (MQ=255)

                cATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGtt                                                                                                                           <  4:59167/141‑1 (MQ=255)

                cATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGtt                                                                                                                           <  1:673244/141‑1 (MQ=255)

                cATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGtt                                                                                                                           >  1:594686/1‑141 (MQ=255)

                 aTCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTa                                                                                                                          >  3:165189/1‑141 (MQ=255)

                  tCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTAc                                                                                                                         <  1:101728/141‑1 (MQ=255)

                  tCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCAAAATCTATAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATAAGTACCCTCTTTTGTTAc                                                                                                                         <  2:363111/141‑1 (MQ=255)

                    cAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATAAGTACCCTCTTTTGTTACGc                                                                                                                       <  2:470699/141‑1 (MQ=255)

                         gttgttCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCt                                                                                                                  <  1:465733/141‑1 (MQ=255)

                          ttgttCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTa                                                                                                                 <  4:286173/141‑1 (MQ=255)

                            gttCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAat                                                                                                               <  1:508768/141‑1 (MQ=255)

                              tCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAatat                                                                                                             <  1:35940/141‑1 (MQ=255)

                                 aTTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATAtt                                                                                                          <  1:474480/141‑1 (MQ=255)

                                      aTGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTa                                                                                                     >  1:753327/1‑141 (MQ=255)

                                      aTGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTa                                                                                                     >  2:676035/1‑141 (MQ=255)

                                      aTGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTa                                                                                                     >  1:705033/1‑141 (MQ=255)

                                        gTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTaaa                                                                                                   >  4:323326/1‑141 (MQ=255)

                                         ttttATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAAt                                                                                                  >  3:215096/1‑141 (MQ=255)

                                              taAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCatat                                                                                             <  1:236362/141‑1 (MQ=255)

                                                aaTTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAAACATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTg                                                                                           <  1:226507/141‑1 (MQ=255)

                                                 aTTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGa                                                                                          >  2:793213/1‑141 (MQ=255)

                                                  ttGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGaa                                                                                         >  3:54744/1‑141 (MQ=255)

                                                  ttGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGaa                                                                                         >  1:598196/1‑141 (MQ=255)

                                                   tGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAac                                                                                        <  2:414541/141‑1 (MQ=255)

                                                    gTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAaca                                                                                       >  2:683209/1‑141 (MQ=255)

                                                    gTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAaca                                                                                       <  2:1041799/141‑1 (MQ=255)

                                                      tttAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACt                                                                                     <  2:616081/141‑1 (MQ=255)

                                                       ttAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACtt                                                                                    >  2:471402/1‑141 (MQ=255)

                                                        tAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACttt                                                                                   <  1:838391/141‑1 (MQ=255)

                                                        tAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACttt                                                                                   <  4:37614/141‑1 (MQ=255)

                                                         aaaTAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGTCATCTAATATATTTTGTAAATCATATTGAACACtttt                                                                                  <  2:1042458/141‑1 (MQ=255)

                                                         aaaTAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACtttt                                                                                  <  2:54602/141‑1 (MQ=255)

                                                           ttaatGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTaa                                                                                <  2:447449/140‑1 (MQ=255)

                                                              atGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCt                                                                             <  2:746673/141‑1 (MQ=255)

                                                              atGTACTCCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTGTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCt                                                                             >  1:45052/1‑141 (MQ=255)

                                                               tGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCtt                                                                            <  1:306787/141‑1 (MQ=255)

                                                               tGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCtt                                                                            >  2:398627/1‑141 (MQ=255)

                                                               tGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCtt                                                                            >  1:115906/1‑141 (MQ=255)

                                                                 tACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCtttt                                                                          >  4:375872/1‑141 (MQ=255)

                                                                  aCTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTc                                                                         >  1:211851/1‑141 (MQ=255)

                                                                     tCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATAAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGGACACTTTTAAGCTTTTCtat                                                                      >  2:296385/1‑141 (MQ=255)

                                                                      caagTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCtata                                                                     <  4:169318/138‑1 (MQ=255)

                                                                       agttGCGTCATCTATAAGAATGTTTAATTCATATGATTTGTAACCTTTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCtatat                                                                    <  1:1033253/138‑2 (MQ=255)

                                                                        ggTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTataaa                                                                   >  2:462195/1‑141 (MQ=255)

                                                                        ggTGCGGCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTataaa                                                                   >  2:509252/1‑141 (MQ=255)

                                                                          tGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTataaata                                                                 >  2:155217/1‑141 (MQ=255)

                                                                                    ttAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACt                                                       <  2:1071145/141‑1 (MQ=255)

                                                                                      aaaaaTGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTg                                                     <  2:541294/141‑1 (MQ=255)

                                                                                       aaaaTGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGt                                                    >  1:13074/1‑141 (MQ=255)

                                                                                           tGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGc                                                <  2:288808/141‑1 (MQ=255)

                                                                                           tGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGc                                                <  1:793213/141‑1 (MQ=255)

                                                                                            gTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCt                                               >  1:186325/1‑141 (MQ=255)

                                                                                            gTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCt                                               >  3:423763/1‑141 (MQ=255)

                                                                                            gTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCt                                               <  4:353654/141‑1 (MQ=255)

                                                                                             tttAATTCGAATGATTTGTAACCTGTTACCTAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTGAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCtt                                              <  3:225107/141‑1 (MQ=255)

                                                                                              ttAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTa                                             >  1:397750/1‑141 (MQ=255)

                                                                                                aaTTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTaaa                                           <  1:184505/141‑1 (MQ=255)

                                                                                                aaTTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTaaa                                           >  3:260636/1‑141 (MQ=255)

                                                                                                       aTGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACAt                                    >  4:259662/1‑141 (MQ=255)

                                                                                                        tGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACAtt                                   <  3:17204/141‑1 (MQ=255)

                                                                                                              gTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAg                             >  1:58126/1‑141 (MQ=255)

                                                                                                              gTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAg                             >  1:74821/1‑141 (MQ=255)

                                                                                                                 aCCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGaaa                          >  2:408986/1‑141 (MQ=255)

                                                                                                                  ccTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAg                         <  1:64638/141‑1 (MQ=255)

                                                                                                                  ccTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAg                         <  1:709818/141‑1 (MQ=255)

                                                                                                                  ccTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAg                         <  2:677714/141‑1 (MQ=255)

                                                                                                                    tGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAgtt                       >  1:434016/1‑141 (MQ=255)

                                                                                                                     gTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAgttg                      <  2:424055/141‑1 (MQ=255)

                                                                                                                      ttACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAgttgt                     <  2:4675/141‑1 (MQ=255)

                                                                                                                       tACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAgttgtt                    >  2:998945/1‑141 (MQ=255)

                                                                                                                        aCATGAACTGTTTTAACCATCAGTACCCTCTTTTCTTACGCCATCTAATATATTTTGTAAATCATATTGAATACTTTTAAGCTTTTATATAAATAACTTTCTACTTGTTCGCTTAAAATGCCATTCGACAGACAGTTGTTc                   >  4:183400/1‑141 (MQ=255)

                                                                                                                                 gTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGc          <  1:398627/141‑1 (MQ=255)

                                                                                                                                 gTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGc          <  1:190396/141‑1 (MQ=255)

                                                                                                                                   tttAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCaa        >  2:209717/1‑141 (MQ=255)

                                                                                                                                     tAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATc      >  4:285552/1‑141 (MQ=255)

                                                                                                                                     tAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATc      >  1:260520/1‑141 (MQ=255)

                                                                                                                                      aaCCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCa     >  1:15685/1‑141 (MQ=255)

                                                                                                                                       aCCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAg    >  4:129997/1‑141 (MQ=255)

                                                                                                                                       aCCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAg    >  1:1070474/1‑141 (MQ=255)

                                                                                                                                        ccATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGt   <  3:214408/141‑1 (MQ=255)

                                                                                                                                        ccATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGt   <  4:323570/141‑1 (MQ=255)

                                                                                                                                        ccATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGt   <  1:937861/141‑1 (MQ=255)

                                                                                                                                         cATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTa  >  1:385942/1‑141 (MQ=255)

ACCCATTCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTA  >  USA300TCH1516_ALE/1517232‑1517509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`