Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,519,965 A→G 6.1% T363T (ACA→ACG ponA → Penicillin‑binding protein 1A/1B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,519,9650AG 6.1% 354.9 / 9.9 120T363T (ACA→ACGponAPenicillin‑binding protein 1A/1B
Reads supporting (aligned to +/- strand):  ref base A (47/65);  new base G (2/5);  total (49/71)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01

Only 100 of 127 total aligned reads displayed.

AAAGATGAAAATTTAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCTTAGCGTATGGACCTGCCAT  >  USA300TCH1516_ALE/1519825‑1520105
                                                                                                                                            |                                                                                                                                            
aaaGATGAAAATTTAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGcaaca                                                                                                                                              <  3:179598/141‑1 (MQ=255)
 aaaaTGAAAATTTAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAAAAGAAAAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGcaacaa                                                                                                                                             <  4:189802/138‑1 (MQ=255)
 aaGATGACAATTTAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGAAATTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGcaacaa                                                                                                                                             <  2:1030318/141‑1 (MQ=255)
 aaGATGAAAATTTAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGcaacaa                                                                                                                                             <  2:1030513/141‑1 (MQ=255)
   gATGAAAATTTAGGTAATGTATTACAAAATGGTATTAAAATTTATACAAACATGGAAAAAAAAAAAAAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAAtt                                                                                                                                           <  2:291187/141‑1 (MQ=255)
       aaaaTTTAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACGATTCTTg                                                                                                                                       <  4:163269/141‑1 (MQ=255)
         aaTTTAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGAt                                                                                                                                     >  2:982660/1‑141 (MQ=255)
         aaTTTAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGAt                                                                                                                                     >  3:443053/1‑141 (MQ=255)
            ttAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGt                                                                                                                                  <  2:811243/141‑1 (MQ=255)
             tAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTa                                                                                                                                 <  1:801209/141‑1 (MQ=255)
             tAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTa                                                                                                                                 <  1:646100/141‑1 (MQ=255)
               ggTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTaaa                                                                                                                               >  1:100632/1‑141 (MQ=255)
               ggTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTaaa                                                                                                                               >  2:701759/1‑141 (MQ=255)
                gTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTaaaa                                                                                                                              <  4:27537/141‑1 (MQ=255)
                         tACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGtt                                                                                                                     <  1:360307/141‑1 (MQ=255)
                              aGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGtt                                                                                                                >  1:1012830/1‑141 (MQ=255)
                                 ggTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCt                                                                                                             <  1:874105/141‑1 (MQ=255)
                                   tATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCtat                                                                                                           <  1:33135/141‑1 (MQ=255)
                                    aTTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCtata                                                                                                          <  2:921027/141‑1 (MQ=255)
                                     ttAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTatat                                                                                                         <  3:172032/141‑1 (MQ=255)
                                            ttATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATCAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCtggtgg                                                                                                  <  1:934270/141‑1 (MQ=255)
                                            ttATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCtggtgg                                                                                                  <  2:981171/141‑1 (MQ=255)
                                            ttATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCtggtgg                                                                                                  <  1:451495/141‑1 (MQ=255)
                                             tataCAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGa                                                                                                 <  2:596112/141‑1 (MQ=255)
                                              ataCAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGAc                                                                                                >  1:606169/1‑141 (MQ=255)
                                              ataCAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGAc                                                                                                >  3:407219/1‑141 (MQ=255)
                                              ataCAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGAc                                                                                                >  3:351089/1‑141 (MQ=255)
                                               taCAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACg                                                                                               <  1:703496/141‑1 (MQ=255)
                                                aCAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGt                                                                                              <  1:492253/141‑1 (MQ=255)
                                                aCAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGt                                                                                              >  1:115257/1‑141 (MQ=255)
                                                 cAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTg                                                                                             <  2:159424/141‑1 (MQ=255)
                                                 cAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTg                                                                                             <  1:120719/141‑1 (MQ=255)
                                                   aaCATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGAt                                                                                           <  2:537838/141‑1 (MQ=255)
                                                   aaCATGAATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTTGACGTGAt                                                                                           <  2:914440/141‑1 (MQ=255)
                                                       tGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCa                                                                                       <  1:116133/141‑1 (MQ=255)
                                                        ggATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCaa                                                                                      >  2:994281/1‑141 (MQ=255)
                                                         gATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCaaa                                                                                     >  4:402956/1‑141 (MQ=255)
                                                          aTAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAg                                                                                    <  1:354174/141‑1 (MQ=255)
                                                           tAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGa                                                                                   >  3:167096/1‑141 (MQ=255)
                                                             aaGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGAcg                                                                                 <  3:266100/141‑1 (MQ=255)
                                                               gATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGAcgtc                                                                               >  4:302838/1‑141 (MQ=255)
                                                                 tGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGAcgtcgt                                                                             >  2:1009031/1‑141 (MQ=255)
                                                                 tGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGAcgtcgt                                                                             >  4:38466/1‑141 (MQ=255)
                                                                   ttCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTa                                                                           >  1:546939/1‑141 (MQ=255)
                                                                       aaaaaCATTACAAAATGCTGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAg                                                                       <  3:109687/141‑1 (MQ=255)
                                                                        aaaaCATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGa                                                                      >  2:27896/1‑141 (MQ=255)
                                                                          aaCATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGaaa                                                                    <  2:535357/141‑1 (MQ=255)
                                                                           aCATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAAc                                                                   <  1:466480/141‑1 (MQ=255)
                                                                           aCATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAAc                                                                   >  2:451932/1‑141 (MQ=255)
                                                                             aTTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACGATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCa                                                                 <  1:437235/141‑1 (MQ=255)
                                                                             aTTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATGCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCa                                                                 <  1:1027253/141‑1 (MQ=255)
                                                                               tACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAg                                                               <  2:772752/141‑1 (MQ=255)
                                                                               tACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAg                                                               <  1:440774/141‑1 (MQ=255)
                                                                               tACAAAATGATGTTGATAATGGTAGCCTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAg                                                               <  2:1074603/141‑1 (MQ=255)
                                                                               tACAAAATGATGTTGATAATAGTAGCTTCTACAAGAAGTAAGAACAACAAGTTGGTGCAACAATGCTTGATAGTAAGACTGGTAGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGCTAACAGTAACCAAg                                                               <  2:335692/141‑1 (MQ=255)
                                                                                aCAAAATGATGTTGATAATGGTAGCTTCTACCAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGc                                                              >  1:126746/1‑141 (MQ=255)
                                                                                aCAAAATGATGTTGATAATGGTAGCTTCTACAAGACTAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGCGGTTTAGTTGCTATATCTGGTGGACGTGATTTCCAAGACGTCGTTAACAGAAACCAAGc                                                              >  2:246207/1‑141 (MQ=255)
                                                                                 cAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCa                                                             >  2:680640/1‑141 (MQ=255)
                                                                                  aaaaTGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCaa                                                            >  1:696638/1‑141 (MQ=255)
                                                                                         tgTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATcc                                                     <  3:65719/141‑1 (MQ=255)
                                                                                          gTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCt                                                    <  2:564531/141‑1 (MQ=255)
                                                                                          gTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCt                                                    <  2:564633/141‑1 (MQ=255)
                                                                                                aaTGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCt                                              <  4:49482/141‑1 (MQ=255)
                                                                                                  tGGTAGCTTCTACAAGAATTAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCtcc                                            <  2:482679/141‑3 (MQ=255)
                                                                                                   ggTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACt                                           <  2:5730/141‑1 (MQ=255)
                                                                                                      atcTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACGATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTggg                                        >  2:1024596/3‑140 (MQ=255)
                                                                                                      aGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGt                                        <  1:793841/141‑1 (MQ=255)
                                                                                                       gCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACGATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGtt                                       <  1:468887/141‑1 (MQ=255)
                                                                                                         ttCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCa                                     <  1:335630/141‑1 (MQ=255)
                                                                                                         ttCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCa                                     >  4:446155/1‑141 (MQ=255)
                                                                                                          tCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCAt                                    <  3:283305/141‑1 (MQ=255)
                                                                                                           cTACAAGAATAGAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATc                                   >  2:12481/1‑141 (MQ=255)
                                                                                                           cTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGATTCATc                                   >  2:974171/1‑141 (MQ=255)
                                                                                                             aCAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCtt                                 >  2:750135/1‑141 (MQ=255)
                                                                                                               aaGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTa                               <  1:278761/141‑1 (MQ=255)
                                                                                                               aaGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTa                               <  1:695184/141‑1 (MQ=255)
                                                                                                                 gAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTaaa                             <  1:1073044/141‑1 (MQ=255)
                                                                                                                  aaTAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTaaaa                            <  1:253763/141‑1 (MQ=255)
                                                                                                                    tAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAAcc                          <  2:339343/141‑1 (MQ=255)
                                                                                                                     aaaGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCt                         >  2:141134/1‑141 (MQ=255)
                                                                                                                      aaGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCtt                        >  4:288295/1‑141 (MQ=255)
                                                                                                                       aGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCttt                       <  1:982499/141‑1 (MQ=255)
                                                                                                                       aGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCttt                       <  2:946800/141‑1 (MQ=255)
                                                                                                                        gACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTc                      <  4:414801/141‑1 (MQ=255)
                                                                                                                        gACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTc                      >  2:761753/1‑141 (MQ=255)
                                                                                                                        gACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTc                      >  4:408792/1‑141 (MQ=255)
                                                                                                                         aCCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCt                     <  2:235742/141‑1 (MQ=255)
                                                                                                                          ccAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCtt                    >  1:985406/1‑141 (MQ=255)
                                                                                                                           caacaaGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCTTa                   >  1:260739/1‑141 (MQ=255)
                                                                                                                              caaGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCTTAGCg                <  3:412253/141‑1 (MQ=255)
                                                                                                                              caaGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCTTAGCg                <  4:5486/141‑1 (MQ=255)
                                                                                                                               aaGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCTTAGCGt               >  4:179191/1‑141 (MQ=255)
                                                                                                                                aGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCTTAGCGTa              <  2:713364/141‑1 (MQ=255)
                                                                                                                                aGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCTTAGCGTa              >  1:70807/1‑141 (MQ=255)
                                                                                                                                aGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCTTAGCGTa              <  1:972740/141‑1 (MQ=255)
                                                                                                                                 gTTGGTGCAACAAGTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGTACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTNGTTCATCTTTAANACCTTTCTTAGCGTAt             <  3:331119/141‑1 (MQ=255)
                                                                                                                                  ttGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCTTAGCGTATg            >  1:155167/1‑141 (MQ=255)
                                                                                                                                       gCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCTTAGCGTATGGACCt       <  1:32227/141‑1 (MQ=255)
                                                                                                                                       gCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCTTAGCGTATGGACCt       <  2:213702/141‑1 (MQ=255)
                                                                                                                                         aacaaTTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCTTAGCGTATGGACCTGc     <  4:351089/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AAAGATGAAAATTTAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTAAAACCTTTCTTAGCGTATGGACCTGCCAT  >  USA300TCH1516_ALE/1519825‑1520105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: