Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,540,113 A→G 6.7% D11D (GAT→GAC ndk ← Nucleoside diphosphate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,540,1130AG 6.7% 405.2 / 12.0 143D11D (GAT→GACndkNucleoside diphosphate kinase
Reads supporting (aligned to +/- strand):  ref base A (65/67);  new base G (3/7);  total (68/75)
Fisher's exact test for biased strand distribution p-value = 3.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

TAAAATGGTTTACCTTGGTGTTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACTTTTCATAAATACTACGTGTTTCTTGAAC  >  USA300TCH1516_ALE/1539973‑1540253
                                                                                                                                            |                                                                                                                                            
taAAATGGTTTACCTTGGTGTTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCa                                                                                                                                              >  1:266168/1‑141 (MQ=255)
  aaaTGGTTTACCTTGGTGTTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATc                                                                                                                                            >  4:46521/1‑141 (MQ=255)
  aaaTGGTTTACCTTGGTGTTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATc                                                                                                                                            >  1:712353/1‑141 (MQ=255)
  aaaTGGTTTACCTTGGTGTTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATc                                                                                                                                            >  2:420836/1‑141 (MQ=255)
  aaaTGGTTTACCTTGGTGTTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCCACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCAtt                                                                                                                                            >  4:391891/1‑140 (MQ=255)
     cggTTTGCCTTGGTGTTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCGTCTgg                                                                                                                                         <  3:34633/140‑1 (MQ=255)
      ggTTTACCTTGGTGTTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGt                                                                                                                                        >  4:456896/1‑141 (MQ=255)
       gTTTACCTTGGTGTTCACCATACTGTGTTTCAGCAAGTTCCATTGGTACTTGCATTACTTTACAACCGACAAGTTTTAGTCCTTTTATTTCAATTCTTGAACTTAATTCAAAAATTAGTTTTCTTTGTACTGCATATGGtt                                                                                                                                       >  4:409760/1‑141 (MQ=255)
       gTTTACCTTGGTGTTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGtt                                                                                                                                       <  3:387803/141‑1 (MQ=255)
            ccTTGGTGTTCACCATACTGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATCCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATc                                                                                                                                  <  2:107694/141‑1 (MQ=255)
              ttGGTGTTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCAt                                                                                                                                <  3:74089/141‑1 (MQ=255)
               tGGTGTTCACCATAATGTGTTTCAGCAAGTTTCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCAtt                                                                                                                               <  2:461970/141‑1 (MQ=255)
                ggTGTTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTa                                                                                                                              >  1:359537/1‑141 (MQ=255)
                  tgtTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTaaa                                                                                                                            >  1:894236/1‑141 (MQ=255)
                  tgtTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTaaa                                                                                                                            >  1:3345/1‑141 (MQ=255)
                   gtTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTaaaa                                                                                                                           >  2:789318/1‑141 (MQ=255)
                      cACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTCCTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATNAAAAATg                                                                                                                        >  2:812004/1‑141 (MQ=255)
                      cACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCGTCTGGTTTAATCATTAAAAATg                                                                                                                        <  1:116321/141‑1 (MQ=255)
                      cACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATg                                                                                                                        >  2:24472/1‑141 (MQ=255)
                       aCCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGATTTAATCATTAAAAAtaa                                                                                                                       >  2:748712/1‑139 (MQ=255)
                          aTAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACg                                                                                                                    <  3:51450/141‑1 (MQ=255)
                           tAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGt                                                                                                                   <  4:412450/141‑1 (MQ=255)
                            atcgtgtTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACATGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGtt                                                                                                                  <  3:183879/138‑1 (MQ=255)
                               gtgtTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTTAATTCTTGAAATTATTTCACTAATTAGATTTCTTTGTACTGGATCTGGTTTAATCATTAAAAATGTACGTTCCa                                                                                                               >  3:224168/1‑141 (MQ=255)
                               gtgtTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCa                                                                                                               <  1:1029486/141‑1 (MQ=255)
                                 gtTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACt                                                                                                             <  1:24472/141‑1 (MQ=255)
                                   ttCAGCCAGTTCCATTCGTCCTCCCCTTAATTTACCACCGACAAGTCTTAGTCCTTTTCTTTCAATTCTTGACATCACTTCACCAATTCCCTTTCTTTGTCCTGCATCTGCTTTAACCATTAACACTGTACCTTCCCCttt                                                                                                           >  2:765644/1‑141 (MQ=255)
                                     cAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGCCAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTAt                                                                                                         >  3:46941/1‑141 (MQ=255)
                                      aGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATg                                                                                                        <  3:264067/141‑1 (MQ=255)
                                         aaGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTAt                                                                                                     >  1:429146/1‑141 (MQ=255)
                                               cATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCt                                                                                               >  4:54632/1‑141 (MQ=255)
                                               cATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCt                                                                                               <  4:149581/141‑1 (MQ=255)
                                                aTTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCtg                                                                                              >  1:437650/1‑141 (MQ=255)
                                                aTTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCtg                                                                                              <  2:542932/141‑1 (MQ=255)
                                                 tgggTACTTGCATTAATTTACCACCGAAAAGTTTTATTCATTTTCTTTCAATTTTTCATATAACTTGACCAATTAGATTTCTTTGTATTGCGTCTGGTATAATCATTAAAAATGTTCGTTCCATTTTATGTATCCCCgtgt                                                                                             >  4:263470/3‑141 (MQ=255)
                                                  tGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGAAAAAAAAAAAAAAATGTACGTTCCACTTTATGTATCCCCCtgtg                                                                                            <  2:240337/141‑1 (MQ=255)
                                                    gTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCGTCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGtt                                                                                          <  2:677449/141‑1 (MQ=255)
                                                     tACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGttt                                                                                         <  2:984011/141‑1 (MQ=255)
                                                     tACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGttt                                                                                         <  1:409886/141‑1 (MQ=255)
                                                       cTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTaa                                                                                       >  1:946354/1‑141 (MQ=255)
                                                       cTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTaa                                                                                       <  1:502415/141‑1 (MQ=255)
                                                         tGCATTATTTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATa                                                                                     <  3:412028/141‑1 (MQ=255)
                                                               aaTTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAGTCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTa                                                                               >  1:742611/1‑141 (MQ=255)
                                                                  ttACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGt                                                                            <  3:55923/141‑1 (MQ=255)
                                                                   tACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCGTCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGta                                                                           <  1:590782/141‑1 (MQ=255)
                                                                   tACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGtt                                                                                                                  <  2:363287/102‑1 (MQ=255)
                                                                   tACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGtt                                                                                                                  >  1:363287/1‑102 (MQ=255)
                                                                   tACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGta                                                                           >  1:841444/1‑141 (MQ=255)
                                                                     ccaccGACAAGTTTTAGTCCTTTTCTTTCAATTCTTTAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGtata                                                                         >  4:291376/1‑141 (MQ=255)
                                                                      caccGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTatat                                                                        <  2:720772/141‑1 (MQ=255)
                                                                         cGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTAc                                                                     <  3:322042/141‑1 (MQ=255)
                                                                         cGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTAc                                                                     >  4:377647/1‑141 (MQ=255)
                                                                          gACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACt                                                                    <  3:392996/141‑1 (MQ=255)
                                                                            cAAGTTTTAGTCCTTTTGTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCTTCTGGTTTAATCATTAAAAATGTACGTTCCTCTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACtat                                                                  <  2:504116/141‑1 (MQ=255)
                                                                             aaGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACtatt                                                                 <  2:479450/141‑1 (MQ=255)
                                                                              aGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACtatta                                                                >  2:604486/1‑141 (MQ=255)
                                                                               gTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACtattat                                                               >  2:120315/1‑141 (MQ=255)
                                                                               gTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACtattat                                                               >  1:587634/1‑141 (MQ=255)
                                                                                ttttAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATtata                                                              <  1:104388/141‑1 (MQ=255)
                                                                                  ttAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATtatata                                                            >  3:16548/1‑141 (MQ=255)
                                                                                   tAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTatatat                                                           >  1:760260/1‑141 (MQ=255)
                                                                                    aGTCCTTTTCTTTCCATTCTTGAAATTTCTTCACCAATTACATTTCTTTGTACTGCATCTGCTCTAATCACTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTANTAGGTTTATGTANATTACTATTATATAtt                                                          <  3:456319/141‑1 (MQ=255)
                                                                                      tCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTaa                                                        >  2:509669/1‑141 (MQ=255)
                                                                                         ttttCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAg                                                     <  2:87153/141‑1 (MQ=255)
                                                                                            tctttcAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGtt                                                  <  2:396976/141‑1 (MQ=255)
                                                                                                tcAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTa                                              <  2:437650/141‑1 (MQ=255)
                                                                                                tcAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTa                                              <  1:371538/141‑1 (MQ=255)
                                                                                                tcAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTa                                              <  3:438450/141‑1 (MQ=255)
                                                                                                tcAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTa                                              <  1:273652/141‑1 (MQ=255)
                                                                                                     tCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAgcg                                         <  1:793236/141‑1 (MQ=255)
                                                                                                       ttGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCt                                       >  2:1065955/1‑141 (MQ=255)
                                                                                                       ttGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCt                                       >  1:707663/1‑141 (MQ=255)
                                                                                                        tGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGTTTGGTAAAGCGCtt                                      >  4:66881/1‑141 (MQ=255)
                                                                                                        tGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCtt                                      >  1:853/1‑141 (MQ=255)
                                                                                                        tGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCtt                                      <  2:593308/141‑1 (MQ=255)
                                                                                                         gAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCttt                                     >  2:903953/1‑141 (MQ=255)
                                                                                                          aaaTTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTc                                    <  2:170423/141‑1 (MQ=255)
                                                                                                             ttACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAAt                                 <  2:132711/141‑1 (MQ=255)
                                                                                                              tACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATa                                >  1:459697/1‑141 (MQ=255)
                                                                                                              tACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATa                                >  2:68240/1‑141 (MQ=255)
                                                                                                                cTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACt                              <  3:159709/141‑1 (MQ=255)
                                                                                                                cTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACt                              <  1:257049/141‑1 (MQ=255)
                                                                                                                cTTCACCAATCACTTTCCCTTGTACGGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACt                              <  1:874377/141‑1 (MQ=255)
                                                                                                                 ttCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTACAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATAGATTAACAGGTTGGTAAAGCGCTTTCAATGCtt                             >  1:621042/1‑141 (MQ=255)
                                                                                                                  tCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTa                                                                                                          >  1:53800/1‑63 (MQ=255)
                                                                                                                  tCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTa                                                                                                          <  2:53800/63‑1 (MQ=255)
                                                                                                                  tCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACttt                            >  1:144833/1‑141 (MQ=255)
                                                                                                                   cACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACtttt                           >  4:422493/1‑141 (MQ=255)
                                                                                                                       aaTTAGATTTCTTTGTACTGCGTCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACTTTTCATa                       <  1:517947/141‑1 (MQ=255)
                                                                                                                          tAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACTTTTCATAAAt                    >  2:417529/1‑141 (MQ=255)
                                                                                                                          tAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACTTTTCATAAAt                    <  2:1046086/141‑1 (MQ=255)
                                                                                                                             aTTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACTTTTCATAAAtact                 <  1:604486/141‑1 (MQ=255)
                                                                                                                               ttCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACTTTTCATAAAtactac               <  2:172143/141‑1 (MQ=255)
                                                                                                                               ttCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACTTTTCATAAAtactac               <  3:423681/141‑1 (MQ=255)
                                                                                                                                tCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTACCATGTTGGTAAAGCGCTTTCAATTCTTTTCATAAATACTACg              <  4:438318/141‑1 (MQ=255)
                                                                                                                                  tttGTTCTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACTTTTCATAAATACTACgtg            <  2:119533/141‑1 (MQ=255)
                                                                                                                                      tACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACTTTTCATAAATACTACGTGtttt        >  4:389401/1‑140 (MQ=255)
                                                                                                                                        cTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCGCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACTTTTCATAAATACTACGTGTTTCtt      >  1:1027162/1‑141 (MQ=255)
                                                                                                                                         tGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAATCGCTTTCAATACTTTTCATAAATACTACGTGTTTCTTg     <  1:573773/141‑1 (MQ=255)
                                                                                                                                           cATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACTTTTCATAAATACTACGTGTTTCTTGaa   >  3:134995/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TAAAATGGTTTACCTTGGTGTTCACCATAATGTGTTTCAGCAAGTTCCATTGGTACTTGCATTAATTTACCACCGACAAGTTTTAGTCCTTTTCTTTCAATTCTTGAAATTACTTCACCAATTAGATTTCTTTGTACTGCATCTGGTTTAATCATTAAAAATGTACGTTCCACTTTATGTATCCCCCTGTGTTTAATAGGTTTATGTATATTACTATTATATATTAACAGGTTGGTAAAGCGCTTTCAATACTTTTCATAAATACTACGTGTTTCTTGAAC  >  USA300TCH1516_ALE/1539973‑1540253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: