Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,627,378 G→T 5.3% I365I (ATC→ATA gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,627,3780GT 5.3% 428.0 / 7.3 128I365I (ATC→ATAgnd6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base G (55/65);  new base T (4/3);  total (59/69)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 133 total aligned reads displayed.

GAACACCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGAATTTT  >  USA300TCH1516_ALE/1627239‑1627518
                                                                                                                                           |                                                                                                                                            
gAACACCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGa                                                                                                                                             <  2:251498/141‑1 (MQ=255)
gAACACCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGa                                                                                                                                             <  1:448475/141‑1 (MQ=255)
gAACACCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGa                                                                                                                                             <  2:70437/141‑1 (MQ=255)
  acacCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATa                                                                                                                                           >  4:46144/1‑141 (MQ=255)
   cacCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATaa                                                                                                                                          <  1:142547/141‑1 (MQ=255)
   cacCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATaa                                                                                                                                          >  2:584796/1‑141 (MQ=255)
   cacCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATaa                                                                                                                                          >  2:591329/1‑141 (MQ=255)
     ccAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATg                                                                                                                                        >  1:265157/1‑141 (MQ=255)
     ccAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATg                                                                                                                                        <  2:1058891/141‑1 (MQ=255)
      cAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGc                                                                                                                                       <  4:341986/141‑1 (MQ=255)
       agatGCGACAACATCACGGAACGCATCTAGATATTCAGTAACGATGTTTTTAAATTAAGCCTCAAGCAATAAGTCAAGTATTACTAGATGATTATCGTAGGCATCTATAATCTTTTGTAAGAAGAGTGCACGTATCATGct                                                                                                                                      >  4:212639/4‑140 (MQ=255)
          tGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAGTCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAAcc                                                                                                                                   >  4:86071/1‑141 (MQ=255)
          tGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAAcc                                                                                                                                   <  4:189280/141‑1 (MQ=255)
           gCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCt                                                                                                                                  <  1:862618/141‑1 (MQ=255)
           gCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCt                                                                                                                                  >  3:270720/1‑141 (MQ=255)
            cGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCtt                                                                                                                                 >  2:807086/1‑141 (MQ=255)
              acaacaTCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTtct                                                                                                                               >  1:37946/1‑141 (MQ=255)
               caacaTCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTtctc                                                                                                                              >  2:856462/1‑141 (MQ=255)
                aacaTCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTtctct                                                                                                                             <  3:117352/141‑1 (MQ=255)
                 acaTCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTtctctc                                                                                                                            <  2:984807/141‑1 (MQ=255)
                   aTCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCa                                                                                                                          <  1:108480/141‑1 (MQ=255)
                    tCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCaa                                                                                                                         <  1:968361/141‑1 (MQ=255)
                        gTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCa                                                                                                                     >  3:209318/1‑141 (MQ=255)
                           aCGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAg                                                                                                                  >  2:350796/1‑141 (MQ=255)
                            cGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGc                                                                                                                 >  1:364332/1‑141 (MQ=255)
                              cATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTa                                                                                                               >  1:448087/1‑141 (MQ=255)
                                 cTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAAt                                                                                                            <  2:753146/141‑1 (MQ=255)
                                   tGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCa                                                                                                          <  2:687961/141‑1 (MQ=255)
                                        ttCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAg                                                                                                     >  4:356049/1‑141 (MQ=255)
                                         tCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATATTTACTCTTTCGCCAGAATTGTGAAAGGATACTGCAACCTTCTCTACAAATAATAGATAAATCACCAAGt                                                                                                    >  3:108774/1‑141 (MQ=255)
                                          cAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGTATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGtt                                                                                                   >  1:992741/1‑141 (MQ=255)
                                            gTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTTTCCAAATCATAGCTAAATCACCAAGtttt                                                                                                 >  4:53188/1‑141 (MQ=255)
                                              aaCGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGTATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTaa                                                                                               >  1:81600/1‑141 (MQ=255)
                                              aaCGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTaa                                                                                               <  1:680602/141‑1 (MQ=255)
                                                 gATGTTTTTAAAGTAAGGATCTAGCAATCAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAAtt                                                                                            <  2:788997/141‑1 (MQ=255)
                                                 gATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTTATCCTTGATTATTATCGTAAGAATCTTTGCTCTTCTGTAAGAATTGTGCACGCATAATGCAACCTTCTCTCCATCTCATGGCTAAATCACCAGGCTTTaaaat                                                                                            <  4:135348/141‑3 (MQ=255)
                                                 gATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAAtt                                                                                            >  1:335785/1‑141 (MQ=255)
                                                 gATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAAtt                                                                                            <  3:444471/141‑1 (MQ=255)
                                                 gATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAAtt                                                                                            <  3:4733/141‑1 (MQ=255)
                                                 gATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAAtt                                                                                            <  4:387455/141‑1 (MQ=255)
                                                   tGTTTTTAAAGTAATGAGTTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCTTCCTTCTCTCCCAATCATAGCTAAATCACCAATTTTTAAATTcc                                                                                          <  4:402337/141‑1 (MQ=255)
                                                    gTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCca                                                                                         >  3:218165/1‑141 (MQ=255)
                                                     tttttAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCcat                                                                                        >  3:20439/1‑141 (MQ=255)
                                                     tcctcaaaGTAAGGATCTAGCAAAATGTTATGTAATCCTGGATCATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGGGCACGGATAGTGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATCCcat                                                                                        >  4:411150/6‑141 (MQ=255)
                                                       tttAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGTATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCcattc                                                                                      <  2:526820/141‑1 (MQ=255)
                                                         tAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCACGTTTTAAATTCcattcat                                                                                    >  2:69331/1‑141 (MQ=255)
                                                                  gATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCAc                                                                           >  1:984073/1‑141 (MQ=255)
                                                                  gATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCAc                                                                           <  1:86398/141‑1 (MQ=255)
                                                                    tCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACtt                                                                         <  1:269324/141‑1 (MQ=255)
                                                                    tCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACtt                                                                         >  1:528614/1‑141 (MQ=255)
                                                                      tAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTGTCCAAGTCGGAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGc                                                                       >  1:213690/1‑141 (MQ=255)
                                                                       aGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCt                                                                      >  1:673446/1‑141 (MQ=255)
                                                                       aGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCt                                                                      >  2:294075/1‑141 (MQ=255)
                                                                           aTAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTAc                                                                  <  2:489170/141‑1 (MQ=255)
                                                                               gTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCAt                                                              >  4:55459/1‑141 (MQ=255)
                                                                                 tttGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCAttt                                                            >  1:716253/1‑141 (MQ=255)
                                                                                    gTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTgcg                                                         <  3:365128/141‑1 (MQ=255)
                                                                                    gTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTgcg                                                         <  1:491917/141‑1 (MQ=255)
                                                                                    gTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTgcg                                                         <  1:961110/141‑1 (MQ=255)
                                                                                    gTAATCCTGGATTATTAGCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAAGCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTgcg                                                         <  1:449574/141‑1 (MQ=255)
                                                                                     tAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTgcgc                                                        <  3:190580/141‑1 (MQ=255)
                                                                                     tAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTgcgc                                                        >  2:208132/1‑141 (MQ=255)
                                                                                      aaTCCTGGATTATTATCATAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGcgcg                                                       <  2:485559/141‑1 (MQ=255)
                                                                                       aTCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTGCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGa                                                      <  1:785877/141‑1 (MQ=255)
                                                                                            tgATTATTATCGTAATCATCTTTGATCTGTTGTAAGAATTGGTCACGTAGAATTCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCt                                                 <  1:99013/140‑1 (MQ=255)
                                                                                            ggATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCt                                                 <  1:1056870/141‑1 (MQ=255)
                                                                                               ttattaTCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTtgt                                              <  1:307879/141‑1 (MQ=255)
                                                                                                   tatCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCAt                                          <  3:371068/141‑1 (MQ=255)
                                                                                                   tatCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTAGACGATACCTTGTGCAt                                          >  1:332437/1‑141 (MQ=255)
                                                                                                    atCGTAAACATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATa                                         >  3:401686/1‑141 (MQ=255)
                                                                                                          aGCATCTTTGATCTTTTGTAAGAATTGTGCACGTATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACa                                   <  4:180011/141‑1 (MQ=255)
                                                                                                          aGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACa                                   <  1:796792/141‑1 (MQ=255)
                                                                                                            cATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACaaa                                 >  2:255336/1‑141 (MQ=255)
                                                                                                             aTCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAAt                                <  2:885916/141‑1 (MQ=255)
                                                                                                             aTCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAAt                                <  3:383293/141‑1 (MQ=255)
                                                                                                             aTCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAAt                                <  1:14992/141‑1 (MQ=255)
                                                                                                              tCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAAtt                               >  1:267651/1‑141 (MQ=255)
                                                                                                                 ttGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTa                            >  1:7326/1‑141 (MQ=255)
                                                                                                                    aTCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTc                         <  3:252860/141‑1 (MQ=255)
                                                                                                                    aTCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTc                         <  2:1051292/141‑1 (MQ=255)
                                                                                                                      cTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCat                       >  2:500037/1‑141 (MQ=255)
                                                                                                                        tttGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCatat                     >  1:387827/1‑141 (MQ=255)
                                                                                                                         ttGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCatata                    <  1:1086280/141‑1 (MQ=255)
                                                                                                                         ttGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCatata                    <  3:181506/141‑1 (MQ=255)
                                                                                                                          tGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCCAGTTTTAAATTCCGTTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCatatat                   <  1:860772/141‑1 (MQ=255)
                                                                                                                          tGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCatatat                   <  3:208744/141‑1 (MQ=255)
                                                                                                                              aGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATg               >  4:199883/1‑141 (MQ=255)
                                                                                                                                 aTTGTGCACGTATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCtt            >  1:471827/1‑141 (MQ=255)
                                                                                                                                   tgtgCACGGATAATGCAACCTTCTCTCCAGATCATATCTAAATCACCAAGTTTTATATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTa          <  2:103463/141‑1 (MQ=255)
                                                                                                                                    gtgCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATCTAATGCTTTAc         >  1:735628/1‑141 (MQ=255)
                                                                                                                                    gtgCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTAc         <  4:292765/141‑1 (MQ=255)
                                                                                                                                    gtgCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTAc         <  2:827979/141‑1 (MQ=255)
                                                                                                                                    gtgCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTAc         <  1:675982/141‑1 (MQ=255)
                                                                                                                                      gCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGa       >  4:18446/1‑141 (MQ=255)
                                                                                                                                       cACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGaa      >  1:206238/1‑141 (MQ=255)
                                                                                                                                         cGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTCGCCCGAAACCTTGTGCATAAGACCAAATTTTACTCATATATAACGCTCCACGAAtt    >  4:414684/1‑141 (MQ=255)
                                                                                                                                           gATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGAAtttt  <  4:306428/141‑1 (MQ=255)
                                                                                                                                           gATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGAAtttt  <  2:448087/141‑1 (MQ=255)
                                                                                                                                           gATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGAAtttt  <  2:447674/141‑1 (MQ=255)
                                                                                                                                           gATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGAAtttt  <  3:26935/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GAACACCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGAATTTT  >  USA300TCH1516_ALE/1627239‑1627518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: