Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 109,531 A→G 5.4% K199K (AAA→AAG)  plc → 1‑phosphatidylinositol phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE109,5310AG 5.4% 446.3 / 10.4 149K199K (AAA→AAG) plc1‑phosphatidylinositol phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (74/67);  new base G (6/2);  total (80/69)
Fisher's exact test for biased strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 151 total aligned reads displayed.

GAAATTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATAAAAAAGTTGAAGCTGTTAA  >  USA300TCH1516_ALE/109394‑109671
                                                                                                                                         |                                                                                                                                            
  aaTTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTgg                                                                                                                                         >  1:552244/1‑141 (MQ=255)
   aTTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGt                                                                                                                                        >  1:440467/1‑141 (MQ=255)
   aTTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGt                                                                                                                                        <  4:242416/141‑1 (MQ=255)
    tttttAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTTTTTTTTTAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATACAATAGAATGGCGGGTACGTACATAAAAAGTGGtt                                                                                                                                       >  2:239684/1‑141 (MQ=255)
     ttttAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTa                                                                                                                                      >  4:53944/1‑141 (MQ=255)
     ttttAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTa                                                                                                                                      >  3:189008/1‑141 (MQ=255)
      tttAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACATGAAGTAATTCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGTGTGTACTTACATAAAAATTgtgtat                                                                                                                                     >  4:42419/1‑136 (MQ=255)
      tttAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTAt                                                                                                                                     <  2:149033/141‑1 (MQ=255)
      tttAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTGTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTAt                                                                                                                                     >  1:214458/1‑141 (MQ=255)
        tAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATgg                                                                                                                                   <  3:216304/141‑1 (MQ=255)
        tAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGATACATACATAAAAAGTGGTTATgg                                                                                                                                   <  4:240272/141‑1 (MQ=255)
            gaATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCt                                                                                                                               <  3:51799/141‑1 (MQ=255)
            gaATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCt                                                                                                                               <  4:253276/141‑1 (MQ=255)
             aaTATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTg                                                                                                                              >  2:243976/1‑141 (MQ=255)
             aaTATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTg                                                                                                                              <  1:228332/141‑1 (MQ=255)
              atatTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGa                                                                                                                             >  1:1067448/1‑141 (MQ=255)
              atatTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGa                                                                                                                             >  2:711848/1‑141 (MQ=255)
              atatTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACATACATAAAGAGTGTTTATGGTGCTGa                                                                                                                             >  2:407628/1‑141 (MQ=255)
               tattattatAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACATACATAAAGAGTGGTTATGGTGCTGac                                                                                                                            <  1:994585/141‑1 (MQ=255)
                 ttattatAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGacac                                                                                                                          <  2:912353/141‑1 (MQ=255)
                 ttattatAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACATACATAAAGAGTGGTTATGGTGCTGacac                                                                                                                          >  1:573583/1‑141 (MQ=255)
                  tattatAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACg                                                                                                                         <  3:96150/141‑1 (MQ=255)
                     tataATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCa                                                                                                                      <  3:53944/141‑1 (MQ=255)
                     aataataAACCGCAATACCAGAATCTTTTTTACACAGGAAGTAAAGCGAATCTTACTTTAAAAGAAACGAAAAGAAAAATTGTCCTATTTAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCa                                                                                                                      <  4:138838/140‑1 (MQ=255)
                      ataataACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAg                                                                                                                     >  2:640418/1‑141 (MQ=255)
                      ataataACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAg                                                                                                                     <  2:340038/141‑1 (MQ=255)
                        aataaCCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGATGACACGTCAGGt                                                                                                                   >  2:604019/1‑141 (MQ=255)
                        aataaCCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACATACATAAAGAGTGGTTATGGTGCTGACACGTCAGGt                                                                                                                   >  3:430571/1‑141 (MQ=255)
                          taaCGCGCAATATAAGAATCTTTTTTACACAGGGAGGAATGGGACTCCTGCTTTAACAGAAACGAAAGGTAAAATTGTCCTATGCGATCGAATGGGGGAAAAAAAAATAAAAAGTGGTTATGGTGCTGACACGTCAGGTAt                                                                                                                 <  2:538209/141‑1 (MQ=255)
                             cccGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCa                                                                                                              >  1:145927/1‑141 (MQ=255)
                             cccGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCa                                                                                                              >  1:1049931/1‑141 (MQ=255)
                             cccGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCa                                                                                                              <  2:170530/141‑1 (MQ=255)
                                gCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATg                                                                                                           >  2:778141/1‑141 (MQ=255)
                                 cAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATgg                                                                                                          >  1:291208/1‑141 (MQ=255)
                                     atCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAg                                                                                                      <  1:101430/141‑1 (MQ=255)
                                       cAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGAc                                                                                                    >  2:911447/1‑141 (MQ=255)
                                         gAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACaa                                                                                                  >  2:200037/1‑141 (MQ=255)
                                         gAATCTTTTTTACACAGGAAGTAAGGCGAATCCTACTTTAAAAGAGACGGAAGGTAAAATTGTCCTATTGAGTAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTTAGGTATTCAATGGTCAGACaa                                                                                                  >  2:96300/1‑141 (MQ=255)
                                               tttttACAAAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTCCGTACATAAAAAGTGGTTATGGTGCTGCCACGTCAGGTATTCACTGGGCAGACACTGCGAc                                                                                            <  4:269653/141‑1 (MQ=255)
                                                ttttACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACa                                                                                           >  1:140081/1‑141 (MQ=255)
                                                  ttACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACAtt                                                                                         >  2:554172/1‑141 (MQ=255)
                                                   tACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACAttt                                                                                        >  3:350707/1‑141 (MQ=255)
                                                      acaGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGaa                                                                                     >  1:530973/1‑141 (MQ=255)
                                                       caGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGaaa                                                                                    >  1:767608/1‑141 (MQ=255)
                                                       caGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGaaa                                                                                    >  2:985025/1‑141 (MQ=255)
                                                        aGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACATACATAAAGAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAAc                                                                                   <  4:174986/141‑1 (MQ=255)
                                                             gTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGaaaa                                                                              <  1:965213/141‑1 (MQ=255)
                                                                 tGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATtaa                                                                          >  2:668359/1‑141 (MQ=255)
                                                                  gCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATtaat                                                                         >  1:830188/1‑141 (MQ=255)
                                                                   cGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATtaata                                                                        >  2:460712/1‑141 (MQ=255)
                                                                    gAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATtaataa                                                                       >  4:299159/1‑141 (MQ=255)
                                                                       tCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATgg                                                                    >  1:968690/1‑141 (MQ=255)
                                                                       tCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTAAGAAAAAAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATgg                                                                    <  2:776341/141‑1 (MQ=255)
                                                                       gccTACTGTAAAAGGAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATgg                                                                    <  4:351669/140‑1 (MQ=255)
                                                                          tACTTTAAAAGAAACGAAAGGTAAAAATGTCCTATTGAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAACGGGCAGACAAGGCGACATTTGAAACGAAAATTAATAATGGTAg                                                                 <  4:31896/141‑1 (MQ=255)
                                                                            cTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCt                                                               <  3:281407/141‑1 (MQ=255)
                                                                             tttAAAATAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCtt                                                              <  2:198213/141‑1 (MQ=255)
                                                                             tttAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCtt                                                              <  1:720459/141‑1 (MQ=255)
                                                                             tttAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAAACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCtt                                                              <  3:144739/141‑1 (MQ=255)
                                                                              ttAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTa                                                             >  3:273252/1‑141 (MQ=255)
                                                                              ttAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTa                                                             <  1:1001551/141‑1 (MQ=255)
                                                                              ttAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTa                                                             <  2:1069012/141‑1 (MQ=255)
                                                                              ttAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTa                                                             <  1:94824/141‑1 (MQ=255)
                                                                              ttAAAAGAAACGAAAGGGAAAATGGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTa                                                             <  2:431784/141‑1 (MQ=255)
                                                                                aaaaGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTaaa                                                           <  2:825045/141‑1 (MQ=255)
                                                                                aaaaGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTaaa                                                           <  2:625424/141‑1 (MQ=255)
                                                                                 aaaGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAAt                                                          >  1:525856/1‑141 (MQ=255)
                                                                                  aaGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAAtt                                                         >  4:166925/1‑141 (MQ=255)
                                                                                   aGAAACGAAAGTAAAAAGTGTTCTATTCAATAGAAAGGGGGGCAGGTACATAATAAGTGGTTATGGAATTGACAGGTAAGGTATTAAATAGGAAGACAAAACGACATTAAAAACGAAAATTAATAATGGTAGCTTaaaaat                                                        >  2:314500/1‑138 (MQ=255)
                                                                                       aCGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTaaaa                                                    >  4:126478/1‑141 (MQ=255)
                                                                                         gAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAATTTTAAAAGt                                                  <  1:953466/141‑1 (MQ=255)
                                                                                         gAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGt                                                  <  1:711848/141‑1 (MQ=255)
                                                                                         gAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGt                                                  <  3:25322/141‑1 (MQ=255)
                                                                                           aaGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGTTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTAc                                                >  2:103657/1‑141 (MQ=255)
                                                                                           aaGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTAc                                                >  2:3733/1‑141 (MQ=255)
                                                                                           aaGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTAc                                                >  2:904601/1‑141 (MQ=255)
                                                                                           aaGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACATACATAAAGAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTAc                                                >  2:294050/1‑141 (MQ=255)
                                                                                             ggTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACaa                                              <  3:210160/141‑1 (MQ=255)
                                                                                              gTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAg                                             >  1:568763/1‑141 (MQ=255)
                                                                                                aaaaTTGTCCTATTCAATAGAATGGGGGGTACATACATAAAGAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGAt                                           >  2:1069479/1‑141 (MQ=255)
                                                                                                 aaaTTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATg                                          >  1:358091/1‑141 (MQ=255)
                                                                                                   aTTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAg                                        >  3:350669/1‑141 (MQ=255)
                                                                                                       tCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGGGtata                                    <  2:660967/141‑1 (MQ=255)
                                                                                                        ccTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATaa                                   <  1:619381/141‑1 (MQ=255)
                                                                                                           aTTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGa                                <  1:700613/141‑1 (MQ=255)
                                                                                                                  aGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTAt                         >  1:34944/1‑141 (MQ=255)
                                                                                                                  aGAATGGGGGGTACGCACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTAt                         <  1:903118/141‑1 (MQ=255)
                                                                                                                   gAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATg                        >  2:674984/1‑141 (MQ=255)
                                                                                                                   gAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATg                        >  1:616652/1‑141 (MQ=255)
                                                                                                                     aTGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGAt                      >  1:599357/1‑141 (MQ=255)
                                                                                                                     aTGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGAt                      >  3:370594/1‑141 (MQ=255)
                                                                                                                      tGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATa                     <  2:530973/141‑1 (MQ=255)
                                                                                                                        gggggTACGTACATATAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATaaa                   <  1:902576/141‑1 (MQ=255)
                                                                                                                        gggggTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGTGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATaaa                   >  3:243423/1‑141 (MQ=255)
                                                                                                                        gggggTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATaaa                   <  4:10876/141‑1 (MQ=255)
                                                                                                                         ggggTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTAAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATAACAAAAATaaaa                  <  2:331252/141‑1 (MQ=255)
                                                                                                                          gggTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATaaaaa                 >  1:265667/1‑141 (MQ=255)
                                                                                                                                  acATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATAAAAAAGTTGAAg         <  1:426102/141‑1 (MQ=255)
                                                                                                                                    aTAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGAAAAATATGATAAAAAAGTTGAAGCt       <  1:704465/141‑1 (MQ=255)
                                                                                                                                        aaaGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATAAAAAAGTTGAAGCTGTTa   >  2:502586/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                            
GAAATTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATAAAAAAGTTGAAGCTGTTAA  >  USA300TCH1516_ALE/109394‑109671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑