Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,642,186 G→A 5.4% S31S (AGC→AGT nusB ← N utilization substance protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,642,1860GA 5.4% 375.6 / 4.7 119S31S (AGC→AGTnusBN utilization substance protein B
Reads supporting (aligned to +/- strand):  ref base G (66/45);  new base A (2/5);  total (68/51)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

GTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCTTTATTTAATTACTGAAT  >  USA300TCH1516_ALE/1642046‑1642326
                                                                                                                                            |                                                                                                                                            
gTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAg                                                                                                                                              <  1:894296/141‑1 (MQ=255)
    ttAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCAAAGTCTAAATCTGGATTATCGTCTTTAATAAAACTTa                                                                                                                                          <  2:201345/141‑1 (MQ=255)
       ataaACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTCATCg                                                                                                                                       >  1:478194/1‑141 (MQ=255)
         aaaCGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCt                                                                                                                                     <  2:1031077/141‑1 (MQ=255)
          aatgTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCtt                                                                                                                                    >  1:756626/4‑141 (MQ=255)
              tGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCAtt                                                                                                                                <  2:691949/141‑1 (MQ=255)
                cAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTa                                                                                                                              >  1:464792/1‑141 (MQ=255)
                  aTAGTCCAATCTTTTAAATAAGGACTACTTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCACTGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTNATTTATc                                                                                                                            >  2:816084/1‑141 (MQ=255)
                  aTAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATc                                                                                                                            >  4:159316/1‑141 (MQ=255)
                  aTAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATc                                                                                                                            <  1:350760/141‑1 (MQ=255)
                  aTAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATc                                                                                                                            <  1:689088/141‑1 (MQ=255)
                   tAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTaact                                                                                                                           <  3:274587/141‑4 (MQ=255)
                          aTCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATc                                                                                                                    >  1:549474/1‑141 (MQ=255)
                            cTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAAGCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCAc                                                                                                                  <  3:100603/141‑1 (MQ=255)
                                  aaTAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCt                                                                                                            >  1:982973/1‑141 (MQ=255)
                                    tAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTc                                                                                                          >  2:343921/1‑141 (MQ=255)
                                     aaGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCa                                                                                                         <  1:386044/141‑1 (MQ=255)
                                      aGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTCTCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCAt                                                                                                        <  1:551000/141‑1 (MQ=255)
                                       ggACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCAtt                                                                                                       >  3:38990/1‑141 (MQ=255)
                                        gACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCTATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCAttt                                                                                                      >  2:120452/1‑141 (MQ=255)
                                        gACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCAttt                                                                                                      <  1:896286/141‑1 (MQ=255)
                                         aCTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTATTAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTc                                                                                                     <  3:372436/141‑1 (MQ=255)
                                          cTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAACTCACTGTCCTTCCTTTCt                                                                                                    >  1:593558/1‑141 (MQ=255)
                                            aaTTGTCTCGTCTAAGACAGGGTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTaa                                                                                                  >  2:482892/1‑141 (MQ=255)
                                             aTTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAAt                                                                                                 >  1:121226/1‑141 (MQ=255)
                                             aTTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAAt                                                                                                 <  1:4084/141‑1 (MQ=255)
                                              ttGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAAtt                                                                                                >  4:215429/1‑141 (MQ=255)
                                                 tctcGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGaa                                                                                             <  2:311886/141‑1 (MQ=255)
                                                  ctcGTCTAATACAGGTTCGTGACCTTTACCGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGaaa                                                                                            >  2:997893/1‑141 (MQ=255)
                                                      tCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATaaa                                                                                        >  1:903583/1‑141 (MQ=255)
                                                       cTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTCAAATCACTGTCCTTCATTTCTAATTGCAATAAAg                                                                                       >  1:1004611/1‑141 (MQ=255)
                                                          aTACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGttt                                                                                    <  4:43523/141‑1 (MQ=255)
                                                          aTACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGttt                                                                                    >  2:126904/1‑141 (MQ=255)
                                                          aTACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGttt                                                                                    <  3:376720/141‑1 (MQ=255)
                                                          aTACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGttt                                                                                    <  2:110440/141‑1 (MQ=255)
                                                          aTACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGttt                                                                                    <  1:219166/141‑1 (MQ=255)
                                                           tACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTg                                                                                   <  2:53795/141‑1 (MQ=255)
                                                            aCAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGATGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTTTCCTTCATTTCTAATTGAAATAAAGTTTGa                                                                                  >  4:142120/1‑141 (MQ=255)
                                                               ggTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGaaaa                                                                               >  2:766350/1‑141 (MQ=255)
                                                               ggTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGaaaa                                                                               >  2:120953/1‑141 (MQ=255)
                                                               ggTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGaaaa                                                                               >  2:498625/1‑141 (MQ=255)
                                                               ggTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGaaaa                                                                               >  2:916524/1‑141 (MQ=255)
                                                                gTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAg                                                                              >  1:206894/1‑141 (MQ=255)
                                                                  tCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTCCATTTCTAATTGAAATAAAGTTTGAAAAGCt                                                                            <  2:872173/141‑1 (MQ=255)
                                                                    gTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTg                                                                          >  4:368037/1‑141 (MQ=255)
                                                                     tGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGc                                                                         >  2:690501/1‑141 (MQ=255)
                                                                       aCCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCAc                                                                       >  4:180116/1‑141 (MQ=255)
                                                                         cTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTc                                                                     >  2:729204/1‑141 (MQ=255)
                                                                         cTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTc                                                                     >  1:120200/1‑141 (MQ=255)
                                                                            tAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCggg                                                                  <  2:326464/141‑1 (MQ=255)
                                                                             aaCGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGa                                                                 >  1:497602/1‑141 (MQ=255)
                                                                              aCGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTACTTGAAATAAAGTTTGAAAAGCTTGCACTCggggt                                                                <  2:473963/141‑3 (MQ=255)
                                                                              aCGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGAt                                                                <  3:91859/141‑1 (MQ=255)
                                                                              aCGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGAt                                                                <  2:290997/141‑1 (MQ=255)
                                                                              aCGCCAGAAACCAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGAt                                                                <  1:744169/141‑1 (MQ=255)
                                                                                  cAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCtt                                                            >  1:581324/1‑141 (MQ=255)
                                                                                   aGAAACTAGCCAATGAATAAATTCGAAGTCTAACTCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATCTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGGAACGCTTGCACTCGCGATTCtct                                                           >  2:475816/1‑139 (MQ=255)
                                                                                   aGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCttt                                                           >  2:409680/1‑141 (MQ=255)
                                                                                   aGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCttt                                                           >  1:745816/1‑141 (MQ=255)
                                                                                    gAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTa                                                          >  2:306871/1‑141 (MQ=255)
                                                                                      aaCTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACg                                                        <  2:612476/141‑1 (MQ=255)
                                                                                       aCTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGa                                                       >  1:811526/1‑141 (MQ=255)
                                                                                         tAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACt                                                     <  1:130547/141‑1 (MQ=255)
                                                                                          aGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTc                                                    <  1:621308/141‑1 (MQ=255)
                                                                                           gCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCa                                                   >  2:95383/1‑141 (MQ=255)
                                                                                             cAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCAtt                                                 <  3:286861/141‑1 (MQ=255)
                                                                                              aaTGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCAttt                                                >  2:512292/1‑141 (MQ=255)
                                                                                               aTGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAACTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCGCTCGGGATTCTTTACGACTCAtttt                                               <  4:390338/141‑1 (MQ=255)
                                                                                               aTGAATAAATTCAAAGTCTAAATCTGGATTATCGTCTTTAATAAAACTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCAtttt                                               <  4:224864/141‑1 (MQ=255)
                                                                                                 gAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTa                                             >  2:375169/1‑141 (MQ=255)
                                                                                                   aTAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAAc                                           >  4:32041/1‑141 (MQ=255)
                                                                                                       aTTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCGTGCCCTCGGGATTCTTTACGACTCATTTTTAACTCCt                                       >  3:292920/1‑141 (MQ=255)
                                                                                                          caaaGTCTAAATCTGGATTATCGTCTTTAATAAAACTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCa                                    >  3:172285/3‑141 (MQ=255)
                                                                                                          cGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGGTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCa                                    >  1:934915/1‑141 (MQ=255)
                                                                                                          cGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCa                                    >  1:453363/1‑141 (MQ=255)
                                                                                                          cGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCa                                    >  1:21404/1‑141 (MQ=255)
                                                                                                             aGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCGTTCAAAc                                 >  4:407744/1‑141 (MQ=255)
                                                                                                                cTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCCTTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGtt                              <  4:280608/141‑1 (MQ=255)
                                                                                                                cTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGtt                              >  1:134593/1‑141 (MQ=255)
                                                                                                                cTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGtt                              >  1:467773/1‑141 (MQ=255)
                                                                                                                 tAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGttt                             >  2:965085/1‑141 (MQ=255)
                                                                                                                   aaTCTGTATTATCGTCTTTAATAAATCTTATCGCTTCATTTATTGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTGCGTCTCATTTTTAACTCCTTCAAACGTTTGt                           <  2:371910/141‑1 (MQ=255)
                                                                                                                   aaTCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGt                           <  1:257353/141‑1 (MQ=255)
                                                                                                                       tGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTAttt                       >  2:742905/1‑141 (MQ=255)
                                                                                                                        ggATTATCGTCTTTAATAAAACTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTAtttt                      >  1:965111/1‑141 (MQ=255)
                                                                                                                          aTTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTc                    >  1:50138/1‑141 (MQ=255)
                                                                                                                           ttATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCt                   <  1:26508/141‑1 (MQ=255)
                                                                                                                               cGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTACATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTCCGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCtttat               >  1:521554/1‑141 (MQ=255)
                                                                                                                                 tCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCtttattt             <  3:247151/141‑1 (MQ=255)
                                                                                                                                 tCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCtttattt             <  1:583378/141‑1 (MQ=255)
                                                                                                                                  cTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCtttattta            >  4:402367/1‑141 (MQ=255)
                                                                                                                                   tttAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCTTTATTTaa           <  2:756626/141‑1 (MQ=255)
                                                                                                                                    ttAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCTTTATTTAAt          >  3:383495/1‑141 (MQ=255)
                                                                                                                                    ttAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCTTTATTTAAt          <  1:616049/141‑1 (MQ=255)
                                                                                                                                       ataaAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCTTTATTTAATTAc       >  3:240438/1‑141 (MQ=255)
                                                                                                                                       ataaAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCTTTATTTAATTAc       >  1:899063/1‑141 (MQ=255)
                                                                                                                                         aaaGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCTTTATTTAATTACTg     >  4:276387/1‑141 (MQ=255)
                                                                                                                                           aGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCTTTATTTAATTACTGaa   <  2:319528/141‑1 (MQ=255)
                                                                                                                                            gCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCTTTATTTAATTACTGAAt  >  3:69183/1‑141 (MQ=255)
                                                                                                                                            gCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCTTTATTTAATTACTGAAt  >  1:632681/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAAATCACTGTCCTTCATTTCTAATTGAAATAAAGTTTGAAAAGCTTGCACTCGGGATTCTTTACGACTCATTTTTAACTCCTTCAAACGTTTGTATTTTTCTTTATTTAATTACTGAAT  >  USA300TCH1516_ALE/1642046‑1642326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: