BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,680,195	C→T	5.5%	L96L (TTG→TTA)	cdd ←	Cytidine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,680,195	0	C	T	5.5%	336.2 / 6.6	108	L96L (TTG→TTA)	cdd	Cytidine deaminase
Reads supporting (aligned to +/- strand): ref base C (54/48); new base T (3/3); total (57/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

Only 100 of 109 total aligned reads displayed.

ATCCTGATTTATGTTCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCGTTCAGCACATATCGATAATGG  >  USA300TCH1516_ALE/1680056‑1680335

aTCCTGATTTATGTTCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCa                                                                                                                                             >  1:483902/1‑141 (MQ=255)

  ccTGATTTATGTTCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCaaa                                                                                                                                           <  2:113684/141‑1 (MQ=255)

  ccTGATTTATGTTCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCaaa                                                                                                                                           <  2:113496/141‑1 (MQ=255)

   cTGATTTATGTTCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCaaaa                                                                                                                                          >  2:484764/1‑141 (MQ=255)

    tGATTTATGTTCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAAc                                                                                                                                         >  1:1011349/1‑141 (MQ=255)

     gATTTATGTTCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACt                                                                                                                                        >  3:209200/1‑141 (MQ=255)

     gATTTATGTTCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACt                                                                                                                                        >  2:873319/1‑141 (MQ=255)

         tATGTTCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTgac                                                                                                                                    <  1:825679/141‑1 (MQ=255)

         tATGTTCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTgac                                                                                                                                    <  2:895302/141‑1 (MQ=255)

          aTGTTCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTATGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTgacg                                                                                                                                   >  1:549430/1‑141 (MQ=255)

             ttCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACa                                                                                                                                >  1:159330/1‑141 (MQ=255)

             ttCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACa                                                                                                                                >  2:766599/1‑141 (MQ=255)

               ctttCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTTAAAACTTGACGACATg                                                                                                                              <  4:393599/138‑1 (MQ=255)

                tGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGc                                                                                                                             <  3:256083/141‑1 (MQ=255)

                 gTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGca                                                                                                                            <  1:1035222/141‑1 (MQ=255)

                  tCATTTATTCTAAATCCTTTCCTGAAAATCCAAATTGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGcac                                                                                                                           <  3:203505/141‑1 (MQ=255)

                   tatctATTCTAAATCCTTTCCTGAAAATCCAAATGGTCGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGcacc                                                                                                                          <  2:458435/137‑1 (MQ=255)

                   cATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGcacc                                                                                                                          <  4:176950/141‑1 (MQ=255)

                    aTTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTTAAAACTTGACGACATGcacca                                                                                                                         >  4:179560/1‑141 (MQ=255)

                        aTTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAg                                                                                                                     <  2:1082961/141‑1 (MQ=255)

                         ttCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAgg                                                                                                                    <  1:1026923/141‑1 (MQ=255)

                         ttCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAgg                                                                                                                    >  1:323289/1‑141 (MQ=255)

                         ttCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAgg                                                                                                                    >  2:489235/1‑141 (MQ=255)

                         ttCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAgg                                                                                                                    >  2:489129/1‑141 (MQ=255)

                           cTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGtg                                                                                                                  >  2:225860/1‑141 (MQ=255)

                             aaaTCCTTTCCTGAAAATCCAAATGGTAGTAACTCGGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGtgat                                                                                                                >  3:102866/1‑141 (MQ=255)

                              aaTCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGtgatg                                                                                                               >  2:564997/1‑141 (MQ=255)

                                tCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGAc                                                                                                             >  1:365028/1‑141 (MQ=255)

                                tCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGAc                                                                                                             >  2:946932/1‑141 (MQ=255)

                                 ccTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACg                                                                                                            <  1:804465/141‑1 (MQ=255)

                                  cTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACgg                                                                                                           <  1:699551/141‑1 (MQ=255)

                                     tCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGttt                                                                                                        >  3:240365/1‑141 (MQ=255)

                                     tCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGttt                                                                                                        >  2:878383/1‑141 (MQ=255)

                                       cTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTAt                                                                                                      >  1:361037/1‑141 (MQ=255)

                                        tGAAAATCCAAATGGTAGTACCTCTGCGACTGTCATCATAACCATATCTCCTTTATGCTTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCTCAAGGTGATGACGGTTTATc                                                                                                     >  1:411729/1‑141 (MQ=255)

                                           aaaTCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGc                                                                                                  <  3:162117/141‑1 (MQ=255)

                                            aaTCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCa                                                                                                 >  2:269884/1‑141 (MQ=255)

                                               ccAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCt                                                                                              <  1:344396/141‑1 (MQ=255)

                                                cAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACTAGGCAATGACTGTTTATCTGCATCTa                                                                                             >  4:433125/1‑141 (MQ=255)

                                                cAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTa                                                                                             <  2:1016805/141‑1 (MQ=255)

                                                  aaTGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACg                                                                                           >  4:83558/1‑141 (MQ=255)

                                                    tGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGt                                                                                         <  1:308075/141‑1 (MQ=255)

                                                    tGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGt                                                                                         <  2:374369/141‑1 (MQ=255)

                                                     ggTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGtt                                                                                        <  2:506749/141‑1 (MQ=255)

                                                      gtagtaACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTGGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTa                                                                                       <  1:10031/141‑1 (MQ=255)

                                                       tagtaACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTAc                                                                                      >  1:856813/1‑141 (MQ=255)

                                                       tagtaACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTAc                                                                                      <  4:452277/141‑1 (MQ=255)

                                                         gtaACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAg                                                                                    <  2:115644/141‑1 (MQ=255)

                                                          taACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGt                                                                                   >  3:260560/1‑141 (MQ=255)

                                                            aCTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTGATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTa                                                                                 <  1:582904/141‑1 (MQ=255)

                                                            aCTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTa                                                                                 <  1:533330/141‑1 (MQ=255)

                                                             ctctGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTAt                                                                                <  1:159302/141‑1 (MQ=255)

                                                               ctGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTCCAGCTATTg                                                                              >  1:97207/1‑141 (MQ=255)

                                                                       gTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCaaaa                                                                      >  1:32967/1‑141 (MQ=255)

                                                                       gTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCaaaa                                                                      >  3:198797/1‑141 (MQ=255)

                                                                           tcatAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCAc                                                                  >  4:286965/1‑141 (MQ=255)

                                                                               agtcATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCTGATAATTTCTTCGAATCTTGACGACGTGTACCACAAGGTGATGGCTGTTTATCTGCATCTGCTGATACAGTTATTGGTTCTAAATCACCTgg                                                              <  4:29952/138‑1 (MQ=255)

                                                                               aaCCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTgg                                                              <  3:7422/141‑1 (MQ=255)

                                                                                aCCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTggc                                                             <  1:1032465/141‑2 (MQ=255)

                                                                                 ccATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTc                                                            <  2:867716/141‑1 (MQ=255)

                                                                                 ccATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGCTTACAGTTATTGATTCAAAATCACCTGGTc                                                            <  2:975366/141‑1 (MQ=255)

                                                                                 ccATATCTCCTGTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGAACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTc                                                            <  2:133000/141‑1 (MQ=255)

                                                                                   gcatCTACGTTATGATTTGTCATATACACCGGCATATCAACAGCACTTAATTGCTTCACAACTTGACGACGTGCACCACGAGGTGATGACGGTTAATCTGCATCTACGGTTACAGTTATTGCTCCAAAATCACCTGGTCtc                                                          <  4:249589/139‑2 (MQ=255)

                                                                                   atatCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTg                                                          <  1:311596/141‑1 (MQ=255)

                                                                                    tatCTCCTTTATGCTTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATCCACCACAACGTGATGACGGTTTAGCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTTGTCTGt                                                         <  4:116827/141‑1 (MQ=255)

                                                                                    tatCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTTAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGt                                                         >  1:252714/1‑141 (MQ=255)

                                                                                        tcCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTTAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATcc                                                     <  3:280611/141‑1 (MQ=255)

                                                                                         ccTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCt                                                    <  1:141192/141‑1 (MQ=255)

                                                                                            ttATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTga                                                 >  3:46107/1‑141 (MQ=255)

                                                                                            ttATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTga                                                 <  1:759898/141‑1 (MQ=255)

                                                                                              ttGATTAGGCATATCCACATGCATATCGTCATCACATGATCCCTACAAAACTTCACGACATGCACCACAAGGTGATGACGCTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTgaga                                               <  1:168526/140‑1 (MQ=255)

                                                                                                 aTTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAAt                                            >  2:553123/1‑141 (MQ=255)

                                                                                                 aTTTGTCATATACACAGGCATATCATCATCACATAATTCCCTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGaaag                                            <  2:716930/141‑2 (MQ=255)

                                                                                                  tttGTCATATACACAGGCATATCATCATCACATAATTCCTTTAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAAtt                                           >  3:43362/1‑141 (MQ=255)

                                                                                                   ttGTCATATACACAGGCATATCATCATCATATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTTAAAATCACCTGGTCTGTATCCTTGATAAATTg                                          >  4:326082/1‑141 (MQ=255)

                                                                                                    tGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGc                                         >  4:92002/1‑141 (MQ=255)

                                                                                                    tGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGc                                         >  1:392385/1‑141 (MQ=255)

                                                                                                      tCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCg                                       >  1:164243/1‑141 (MQ=255)

                                                                                                       cATATACACAGGCATATCATCATCACATAATTCCTTTAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGa                                      <  1:41265/141‑1 (MQ=255)

                                                                                                        atatACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGAt                                     <  4:235560/141‑1 (MQ=255)

                                                                                                         tataCACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATa                                    >  2:428721/1‑141 (MQ=255)

                                                                                                            acacAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCa                                 >  2:788678/1‑141 (MQ=255)

                                                                                                              acaGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCaaa                               >  1:138682/1‑141 (MQ=255)

                                                                                                              acaGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTAGCCTTGAGAAATTGCCGATACCaaa                               >  4:448868/1‑141 (MQ=255)

                                                                                                               caGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAAc                              >  1:759200/1‑141 (MQ=255)

                                                                                                                  gCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAg                           >  3:205827/1‑141 (MQ=255)

                                                                                                                      atcatcatcACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCg                       >  1:1041769/1‑141 (MQ=255)

                                                                                                                       tcatcatcaCATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCGt                      >  3:384627/1‑141 (MQ=255)

                                                                                                                       tcatcatcaCATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCGt                      >  4:179073/1‑141 (MQ=255)

                                                                                                                          tcatcaCATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATATACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCGTTCa                   >  2:171276/1‑141 (MQ=255)

                                                                                                                            atcaCATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCGTTCAGc                 <  1:905575/141‑1 (MQ=255)

                                                                                                                             tcaCATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCGTTCAGca                >  2:334035/1‑141 (MQ=255)

                                                                                                                              cacaTAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCGTTCAGcac               >  3:342201/1‑141 (MQ=255)

                                                                                                                                caTAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCGTTCAGCACat             <  2:990132/141‑1 (MQ=255)

                                                                                                                                caTAATGCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGATAAATTGCCGATACCAAACTAGCTCGTTCAGCACat             <  4:38952/141‑1 (MQ=255)

                                                                                                                                 aTAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCGTTCAGCACata            <  4:379675/141‑1 (MQ=255)

                                                                                                                                  tAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCGTTCAGCACatat           >  1:550809/1‑141 (MQ=255)

                                                                                                                                      tCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCCGTATCCTTGAGAAATTGCCGATACCAAACCAGCTCGTTCAGCACATATCGAt       <  2:411729/141‑1 (MQ=255)

                                                                                                                                         ttCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCGTTCAGCACATATCGATAAt    >  2:893394/1‑141 (MQ=255)

                                                                                                                                         ttCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCGTTCAGCACATATCGATAAt    <  3:175292/141‑1 (MQ=255)

ATCCTGATTTATGTTCTGTCATTTATTCTAAATCCTTTCCTGAAAATCCAAATGGTAGTAACTCTGCGACTGTCATCATAACCATATCTCCTTTATGATTTGTCATATACACAGGCATATCATCATCACATAATTCCTTCAAAACTTGACGACATGCACCACAAGGTGATGACGGTTTATCTGCATCTACGGTTACAGTTATTGATTCAAAATCACCTGGTCTGTATCCTTGAGAAATTGCCGATACCAAACTAGCTCGTTCAGCACATATCGATAATGG  >  USA300TCH1516_ALE/1680056‑1680335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`