Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 109,964 T→A 5.1% intergenic (+43/‑178) plc → / → USA300HOU_RS00515 1‑phosphatidylinositol phosphodiesterase/putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE109,9640TA 5.1% 403.4 / 10.3 131intergenic (+43/‑178)plc/USA300HOU_RS005151‑phosphatidylinositol phosphodiesterase/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (64/60);  new base A (4/3);  total (68/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01

Only 100 of 134 total aligned reads displayed.

GGAAAGCTAGAACGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATAAATTGATAAATATATGTAAT  >  USA300TCH1516_ALE/109824‑110101
                                                                                                                                             |                                                                                                                                         
ggAAAGCTAGAACGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGAt                                                                                                                                           >  4:137765/1‑141 (MQ=255)
ggAAAGCTAGAACGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGAt                                                                                                                                           >  2:445666/1‑141 (MQ=255)
  aaaGCTAGAACGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGAATGAAAATAGTTAGCTTc                                                                                                                                         >  4:38528/1‑141 (MQ=255)
     gCTAGAACGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTaa                                                                                                                                      <  3:427095/141‑1 (MQ=255)
     gCTAGAACGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTaa                                                                                                                                      <  1:1010469/141‑1 (MQ=255)
      cTAGAACGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAat                                                                                                                                     <  1:42275/141‑1 (MQ=255)
      cTAGAACGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAat                                                                                                                                     <  2:179792/141‑1 (MQ=255)
        aGAACGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAatta                                                                                                                                   >  4:426455/1‑141 (MQ=255)
          aaCGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAattatt                                                                                                                                 >  2:740128/1‑141 (MQ=255)
          aaCGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAagtatt                                                                                                                                 <  2:837054/141‑1 (MQ=255)
           aCGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTAttt                                                                                                                                >  4:5869/1‑141 (MQ=255)
               gTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTAGTGAAAATAATTAGAATCTAACTATTTTCa                                                                                                                             <  1:118242/141‑1 (MQ=39)
               gTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCac                                                                                                                            <  4:339147/141‑1 (MQ=255)
               gTTGGCTGATTGTGGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATATTCac                                                                                                                            <  2:212801/141‑1 (MQ=255)
                  ggCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCactac                                                                                                                         <  2:8107/141‑1 (MQ=255)
                         tgttgACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTAGTGAAAATAATTAGAATCTAACTATTTTCATACTCGtctt                                                                                                                  <  1:53774/141‑4 (MQ=37)
                          gttgACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTAGTGAAAATAATTAGAATCTAACTATTTTCATACTCGtctta                                                                                                                 <  1:734241/141‑5 (MQ=37)
                              aCTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTAttt                                                                                                             >  1:245323/1‑141 (MQ=255)
                              aCTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTAttt                                                                                                             >  1:821956/1‑141 (MQ=255)
                                  tGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGaa                                                                                                         >  2:237789/1‑141 (MQ=255)
                                   gCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGaaa                                                                                                        <  1:1043647/141‑1 (MQ=255)
                                    cAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGaaaa                                                                                                       >  3:185980/1‑141 (MQ=255)
                                    cAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGaaaa                                                                                                       >  4:168647/1‑141 (MQ=255)
                                     aGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAAt                                                                                                      <  1:1072900/141‑1 (MQ=255)
                                        atatACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAg                                                                                                   <  1:668505/141‑1 (MQ=255)
                                          ataCGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGta                                                                                                 >  3:221160/1‑141 (MQ=255)
                                          ataCGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGta                                                                                                 <  3:47115/141‑1 (MQ=255)
                                            aCGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGtaat                                                                                               >  1:593008/1‑141 (MQ=255)
                                             cGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTCTGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGtaata                                                                                              >  3:455453/1‑141 (MQ=255)
                                             cGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGtaata                                                                                              <  1:765903/141‑1 (MQ=255)
                                              gTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGtaataa                                                                                             >  2:527563/1‑141 (MQ=255)
                                               tGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTaataat                                                                                            <  4:175799/141‑1 (MQ=255)
                                                  ccTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTca                                                                                         <  2:246057/141‑1 (MQ=255)
                                                   cTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTcaa                                                                                        >  2:980352/1‑141 (MQ=255)
                                                   cTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAACAATTcaa                                                                                        <  1:857998/141‑1 (MQ=255)
                                                       ttatGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCTATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTAGTTTCACTCCTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAat                                                                                    <  4:306013/140‑1 (MQ=255)
                                                        tatGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAata                                                                                   >  4:145229/1‑141 (MQ=255)
                                                        tatGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAata                                                                                   <  1:17595/141‑1 (MQ=255)
                                                         atgatgATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAatat                                                                                  >  2:1040062/1‑141 (MQ=255)
                                                          tgatgaTATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGACAATAAGTAATAATTCAACAAtatt                                                                                 >  1:555026/1‑141 (MQ=255)
                                                           gatgatATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAAtatta                                                                                <  3:145341/141‑1 (MQ=255)
                                                           gatgatATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAAtatta                                                                                <  3:180748/141‑1 (MQ=255)
                                                            atgatATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAAtattat                                                                               >  4:19099/1‑141 (MQ=255)
                                                               atatCGTAAGTGAAATTATAGATAGTAATACATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATaaa                                                                            >  2:976840/1‑141 (MQ=255)
                                                                    gTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGaa                                                                       >  4:422173/1‑141 (MQ=255)
                                                                    gTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGaa                                                                       <  1:111090/141‑1 (MQ=255)
                                                                    gTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGaa                                                                       >  2:504010/1‑141 (MQ=255)
                                                                     tAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAAc                                                                      <  2:403683/141‑1 (MQ=255)
                                                                      aaGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACa                                                                     >  1:571057/1‑141 (MQ=255)
                                                                      aaGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACa                                                                     >  2:1064782/1‑141 (MQ=255)
                                                                         tGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGAt                                                                  >  2:582779/1‑141 (MQ=255)
                                                                           aaaTTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAATTAATATTTCCACAATATTATAAATTGAACAGAttt                                                                >  2:444947/1‑140 (MQ=255)
                                                                            aaTTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGAttgt                                                               >  1:544519/1‑141 (MQ=255)
                                                                               tataGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGAttgtttg                                                            <  3:426455/141‑1 (MQ=255)
                                                                               tataGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGAttgtttg                                                            <  2:601301/141‑1 (MQ=255)
                                                                                atagatagTAATAAATAAGGATTCAATAATGATATTAAGACTAATATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGAttgtttgt                                                           <  3:346978/141‑1 (MQ=255)
                                                                                atagatagTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACGCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGAttgtttgt                                                           <  1:625218/141‑1 (MQ=255)
                                                                                 tagatagTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTtgtg                                                          <  2:955127/141‑1 (MQ=255)
                                                                                  agatagTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGa                                                         >  1:1049750/1‑141 (MQ=255)
                                                                                  agatagTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGa                                                         <  1:42845/141‑1 (MQ=255)
                                                                                  agatagTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGa                                                         >  1:188242/1‑141 (MQ=255)
                                                                                  agatagTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGa                                                         <  2:716222/141‑1 (MQ=255)
                                                                                  agatagTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGa                                                         >  2:734858/1‑141 (MQ=255)
                                                                                  agataaTAATAAAAAAGGAATCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTAGAAAATAAAAAAAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGa                                                         <  2:408573/141‑1 (MQ=255)
                                                                                     tagTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAAt                                                      <  4:323578/141‑1 (MQ=255)
                                                                                       gTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAAttt                                                    <  1:476336/141‑1 (MQ=255)
                                                                                           taaataaGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGa                                                >  1:834659/1‑141 (MQ=255)
                                                                                            aaataaGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGat                                               <  2:276912/141‑1 (MQ=255)
                                                                                             aataaGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGata                                              >  1:713892/1‑141 (MQ=255)
                                                                                               taaGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGataat                                            >  1:13800/1‑141 (MQ=255)
                                                                                                 aGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAatat                                          >  1:986457/1‑141 (MQ=255)
                                                                                                 aGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAatat                                          >  2:593272/1‑141 (MQ=255)
                                                                                                 aGGATTCAATAACGACATTATGACGAGTATTAAAAAAGTTAGAATCTAATTATTTTCACTACTCGTTTTTAATTTGTAACATAGTAATAAAACAACAATATAATAAATTGAACAGATATTTTATAAAAATTCtaataaaaa                                          >  2:857012/1‑138 (MQ=255)
                                                                                                  ggATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATAtt                                         >  1:986934/1‑141 (MQ=255)
                                                                                                    aTTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTaa                                       >  1:321647/1‑141 (MQ=255)
                                                                                                    aTTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGGGAAATTTTTGATAATATTaa                                       <  4:241357/141‑1 (MQ=255)
                                                                                                       cAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGt                                    >  3:427094/1‑141 (MQ=255)
                                                                                                                 atTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTTAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGATAAAAAgtgt                          >  2:411504/1‑141 (MQ=255)
                                                                                                                 atTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAgtgt                          >  3:68384/1‑141 (MQ=255)
                                                                                                                   tAAGACGAGTATGAAAATATTTAGATTGTAATGGGTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTta                        <  1:762577/141‑1 (MQ=255)
                                                                                                                     aaaCGAGTAGTGAAAATAATTAGAATCTAACTATTTTCATACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATATTTGATAATATTAAAGTGAAAAAGTGTtata                      >  2:256684/3‑141 (MQ=37)
                                                                                                                     aGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTtata                      <  3:112538/141‑1 (MQ=255)
                                                                                                                      gACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAAGTTTTGATAATATTAAAGTGAAAAAGTGTTATaa                     >  3:327023/1‑141 (MQ=255)
                                                                                                                       aCGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTTTTTTTTTTTTTAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATaaa                    >  4:68448/1‑141 (MQ=255)
                                                                                                                       aCGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATaaa                    >  2:636875/1‑141 (MQ=255)
                                                                                                                       aCGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATaaa                    >  1:319546/1‑141 (MQ=255)
                                                                                                                       aCGAGTATGAAAATAGATAGATTCTAATTATTTTCACTACTCGTTTTTATTTAGTAAAACAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGATAAAGTGTTATaac                    >  2:491428/1‑140 (MQ=255)
                                                                                                                         gAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATAAAtt                  <  1:980352/141‑1 (MQ=255)
                                                                                                                           gTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATAAATTGa                <  4:211673/141‑1 (MQ=255)
                                                                                                                           gTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATAAATTGa                <  1:305852/141‑1 (MQ=255)
                                                                                                                             aTGAAAATAGTTAGATTCTAATTATTTTTACTACTCGTTTTTATTTTGAAAATAAGCAATAATTCAACAATATTATAAATTAAACAGATTGTTTGTGAAAATTTTGATAATAATAAAAAGAAAAAGTGTTATAAATTGATa              <  2:935814/141‑1 (MQ=255)
                                                                                                                             aTGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGCAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATAAATTGATa              <  2:805980/141‑1 (MQ=255)
                                                                                                                                 aaaaTAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATAAATTGATAAat           >  1:1072224/1‑141 (MQ=255)
                                                                                                                                  aaaTAGTTAGATTATAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATAAATTGATAAata          <  2:680907/141‑1 (MQ=255)
                                                                                                                                   aaTAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATAAATTGATAAatat         >  1:233269/1‑141 (MQ=255)
                                                                                                                                   aaTAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTAGATAATATTAAAGTGAAAAAGTGTTATAAATTGATAAatat         >  4:257043/1‑141 (MQ=255)
                                                                                                                                       gTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATAAATTGATAAATATATGt     <  3:5869/141‑1 (MQ=255)
                                                                                                                                       gTTAGATTATAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAAAAAAAAAGAAAAAAAGTGTTATAAATTGATAAATATATGt     <  2:303108/141‑1 (MQ=255)
                                                                                                                                         tAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGCAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGTTAATATTAAAGTGAACAAGTGTTATAAATTGATAAATATATGTaa   >  2:630755/1‑141 (MQ=255)
                                                                                                                                          aGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATAAATTGATAAATATATGTAAt  <  1:445666/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                         
GGAAAGCTAGAACGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTAAGTGAAATTATAGATAGTAATAAATAAGGATTCAATAATGATATTAAGACGAGTATGAAAATAGTTAGATTCTAATTATTTTCACTACTCGTTTTTATTTTGAAAATAAGTAATAATTCAACAATATTATAAATTGAACAGATTGTTTGTGAAATTTTTGATAATATTAAAGTGAAAAAGTGTTATAAATTGATAAATATATGTAAT  >  USA300TCH1516_ALE/109824‑110101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: