Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,789,169 A→T 6.0% V117V (GTT→GTA gapA2 ← Glyceraldehyde‑3‑phosphate dehydrogenase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,789,1690AT 6.0% 363.1 / 10.6 118V117V (GTT→GTAgapA2Glyceraldehyde‑3‑phosphate dehydrogenase 2
Reads supporting (aligned to +/- strand):  ref base A (55/55);  new base T (3/4);  total (58/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 125 total aligned reads displayed.

ACTTTTGCAACTGGACCAATGCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCAACCAATTTAATTTT  >  USA300TCH1516_ALE/1789029‑1789309
                                                                                                                                            |                                                                                                                                            
   tttGCAGCTGGACCAATGCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACt                                                                                                                                           <  2:755775/141‑1 (MQ=255)
   tttGCAACTGGACCAATGCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACt                                                                                                                                           >  2:1015770/1‑141 (MQ=255)
    ttGCAACTGGACCAATGCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACtt                                                                                                                                          <  2:300719/141‑1 (MQ=255)
          cTGGACCAATGCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTgg                                                                                                                                    <  3:332599/141‑1 (MQ=255)
           tGGACCAATGCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGc                                                                                                                                   >  2:184337/1‑141 (MQ=255)
              aCCAATGCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTTCGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCAcc                                                                                                                                <  1:1056378/141‑1 (MQ=255)
              aCCAATGCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCAcc                                                                                                                                <  3:422769/141‑1 (MQ=255)
              aCCAATGCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCAcc                                                                                                                                <  2:111262/141‑1 (MQ=255)
              aCCAATGCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCAcc                                                                                                                                <  1:614998/141‑1 (MQ=255)
              aCCAATGCAAATAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTGTTTGGCAcc                                                                                                                                <  1:956176/141‑1 (MQ=255)
               ccAATGCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAATACTTTTTTGGCACCt                                                                                                                               >  2:511455/1‑141 (MQ=255)
                cAATGCAATTAGTAGTACCAATAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTg                                                                                                                              <  1:283778/141‑1 (MQ=255)
                  aTGCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCt                                                                                                                            <  4:211282/141‑1 (MQ=255)
                    gCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCttt                                                                                                                          <  1:530110/141‑1 (MQ=255)
                        ttAGTAGTACATTAAGCATTACTAAAAATGTCATATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTAACATTTTAACATGTCCACCTTTTTAAGGACCAGTTAACAATACTTTTTTGGCACCTGCTTTAata                                                                                                                      <  2:157776/141‑1 (MQ=255)
                        ttAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAata                                                                                                                      <  1:474441/141‑1 (MQ=255)
                         tagtagTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAatat                                                                                                                     >  4:203057/1‑141 (MQ=255)
                           gtagtaCATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATAtgt                                                                                                                   >  1:478763/1‑141 (MQ=255)
                               taCATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATGATTTACGCCTTTAGCGACCATCTGAACATGTCCACCTTTGGAAGGACCAGTTAACGGGTCTTTTTTGGCTTCTGCTTTTATATGTGCGa                                                                                                               <  2:117273/141‑1 (MQ=255)
                               taCATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGa                                                                                                               <  1:361533/141‑1 (MQ=255)
                                 cATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATATCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATg                                                                                                             <  4:358423/141‑1 (MQ=255)
                                  aTGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATgg                                                                                                            <  3:68880/141‑1 (MQ=255)
                                    gAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCt                                                                                                          >  2:355009/1‑141 (MQ=255)
                                     aaGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCtt                                                                                                         <  2:161512/141‑1 (MQ=255)
                                      aGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTAACATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAATACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCttt                                                                                                        >  2:824916/1‑141 (MQ=255)
                                        cATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTAt                                                                                                      >  2:938283/1‑141 (MQ=255)
                                            aCTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCAcc                                                                                                  >  2:563376/1‑141 (MQ=255)
                                            aCTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCAcc                                                                                                  <  2:514226/141‑1 (MQ=255)
                                              tAAAAATGTCAAATGCTTCTATATCTAATTGGTGATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTATTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCAt                                                                                                >  2:724352/1‑141 (MQ=255)
                                                 aaaTGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGAt                                                                                             >  4:122781/1‑141 (MQ=255)
                                                     gTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTaaa                                                                                         >  1:478283/1‑141 (MQ=255)
                                                      tCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGCTGGCTTTATCACCATGATTAAAt                                                                                        >  1:94249/1‑141 (MQ=255)
                                                      tCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAAt                                                                                        <  4:258090/141‑1 (MQ=255)
                                                      tCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAAt                                                                                        >  2:604998/1‑141 (MQ=255)
                                                      tCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAAt                                                                                        <  2:465197/141‑1 (MQ=255)
                                                      tCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAAt                                                                                        >  3:443974/1‑141 (MQ=255)
                                                       cAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAAtt                                                                                       >  2:646644/1‑141 (MQ=255)
                                                       cAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAAtt                                                                                       >  1:978106/1‑141 (MQ=255)
                                                         aaTGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTa                                                                                     >  2:349537/1‑141 (MQ=255)
                                                          aTGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTAc                                                                                    >  2:835444/1‑141 (MQ=255)
                                                             cTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAg                                                                                 <  1:783657/141‑1 (MQ=255)
                                                                cTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTg                                                                              <  3:324993/141‑1 (MQ=255)
                                                                 tataTCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGc                                                                             <  2:187107/141‑1 (MQ=255)
                                                                   tagcTAATTGGTTATCTTTTACGCCTTTGCCTTGCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCATTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCAt                                                                           <  4:122130/138‑1 (MQ=255)
                                                                    atCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATc                                                                          <  2:933487/141‑1 (MQ=255)
                                                                      cTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCta                                                                        <  1:40195/141‑1 (MQ=255)
                                                                         aTTGGTTATCATTTACGCCTTTAACTAACATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAATACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAg                                                                     <  3:354192/141‑1 (MQ=255)
                                                                          ttGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTTTTTTTTTCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCGATAGc                                                                    >  2:577393/1‑141 (MQ=255)
                                                                          ttGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGc                                                                    <  2:1067457/141‑1 (MQ=255)
                                                                          ttGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGc                                                                    <  3:235802/141‑1 (MQ=255)
                                                                            ggTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCaa                                                                  <  2:583108/141‑1 (MQ=255)
                                                                             gTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGCTTAAATTTACCAGTTGCATCTATAGCAat                                                                 >  2:293430/1‑141 (MQ=255)
                                                                             gTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAat                                                                 >  2:293248/1‑141 (MQ=255)
                                                                             gTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAat                                                                 <  2:902800/141‑1 (MQ=255)
                                                                              ttATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAata                                                                >  1:290414/1‑141 (MQ=255)
                                                                               tATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAatat                                                               >  1:236915/1‑141 (MQ=255)
                                                                                aTCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATc                                                              <  3:382263/141‑1 (MQ=255)
                                                                                 tCATTTACGCCTTTAACTAACATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAATACTTTTTTGGCTCCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCg                                                             >  4:169926/1‑141 (MQ=255)
                                                                                      tACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATc                                                        >  2:805935/1‑141 (MQ=255)
                                                                                        cGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTa                                                      >  3:244610/1‑141 (MQ=255)
                                                                                        cGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTa                                                      <  2:589258/141‑1 (MQ=255)
                                                                                         gCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTaa                                                     >  4:330879/1‑141 (MQ=255)
                                                                                           cTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTTTCTTTTTCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAAtt                                                   >  2:366755/1‑141 (MQ=255)
                                                                                           cTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAAtt                                                   >  3:20795/1‑141 (MQ=255)
                                                                                            tttAACTACCATTTGAGCATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTc                                                  <  2:299597/141‑1 (MQ=255)
                                                                                                    ccATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATg                                          >  2:856422/1‑141 (MQ=255)
                                                                                                     cATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATgg                                         <  1:189154/141‑1 (MQ=255)
                                                                                                      aTTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGc                                        <  1:418530/141‑1 (MQ=255)
                                                                                                           aaCATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGTCACTTGCTTTAATCTGTGTGATGGCTTTATCACCATGATTAACTTCCCCAGTCGCATCTATAGCCGTATCGATCTCTCCTTCTTTCCATCGCAAGtt                                   >  2:47822/1‑141 (MQ=255)
                                                                                                           aaCATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGTGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATCTCGATATCTAATTCTTTCCATGGCAAGtt                                   >  2:47669/1‑141 (MQ=255)
                                                                                                            aCATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGttt                                  <  2:857568/141‑1 (MQ=255)
                                                                                                              aTGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTc                                <  2:142417/141‑1 (MQ=255)
                                                                                                               tGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCa                               >  1:174336/1‑141 (MQ=255)
                                                                                                               tGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCa                               >  1:1079704/1‑141 (MQ=255)
                                                                                                                gTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAg                              >  1:938464/1‑141 (MQ=255)
                                                                                                                gTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAg                              >  1:395369/1‑141 (MQ=255)
                                                                                                                gTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAg                              >  2:1027667/1‑141 (MQ=255)
                                                                                                                gTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAg                              <  2:1028113/141‑1 (MQ=255)
                                                                                                                  ccACCTTTTGAAGGACCAGTTAACAATACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGa                            <  1:824916/141‑1 (MQ=255)
                                                                                                                   cACCTTTTGAAGGACCAGTTAACAAAACTTTTTTTTCTTTTTCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGAt                           >  2:495242/1‑141 (MQ=255)
                                                                                                                    aCCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGAtt                          <  1:601209/141‑1 (MQ=255)
                                                                                                                        tttGAAGGACCAGTTAACAAAACTTTTTTTTTACTTTTTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGa                      >  2:798939/1‑141 (MQ=255)
                                                                                                                         ttGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGAt                     >  2:477839/1‑141 (MQ=255)
                                                                                                                           gAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCa                   <  2:499320/141‑1 (MQ=255)
                                                                                                                           gAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCa                   <  2:420717/141‑1 (MQ=255)
                                                                                                                           gAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCa                   >  1:409906/1‑141 (MQ=255)
                                                                                                                            aaGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAg                  >  1:681647/1‑141 (MQ=255)
                                                                                                                               gACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCaa               >  1:967263/1‑141 (MQ=255)
                                                                                                                                aCCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCAAc              >  4:105977/1‑141 (MQ=255)
                                                                                                                                 ccAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCAAcc             <  3:142367/141‑1 (MQ=255)
                                                                                                                                 ccAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAACAAcc             >  1:974901/1‑141 (MQ=255)
                                                                                                                                    gTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCAACCaat          >  2:994936/1‑141 (MQ=255)
                                                                                                                                     ttAACAAATCTTTTTTGGCACCTGTTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCAACCaatt         <  3:440390/141‑1 (MQ=255)
                                                                                                                                     ttAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCAACCaatt         <  4:410520/141‑1 (MQ=255)
                                                                                                                                     ttAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCAACCaatt         <  4:410495/141‑1 (MQ=255)
                                                                                                                                          aaaaCTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATGCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCAACCaatttaatt    <  2:704462/141‑1 (MQ=255)
                                                                                                                                           aaaCTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCAACCaatttaattt   >  4:358210/1‑141 (MQ=255)
                                                                                                                                           aaaCTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCAACCaatttaattt   >  2:1007377/1‑141 (MQ=255)
                                                                                                                                           aaaCTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTCATGATCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCAACCaatttaattt   <  1:556130/141‑1 (MQ=255)
                                                                                                                                            aaCTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCAACCAATTTAAtttt  <  2:751550/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ACTTTTGCAACTGGACCAATGCAATTAGTAGTACATGAAGCATTACTAAAAATGTCAAATGCTTCTATATCTAATTGGTTATCATTTACGCCTTTAACTACCATTTGAACATGTCCACCTTTTGAAGGACCAGTTAACAAAACTTTTTTGGCACCTGCTTTAATATGTGCGATGGCTTTATCACCATGATTAAATTTACCAGTTGCATCTATAGCAATATCGATATCTAATTCTTTCCATGGCAAGTTTTCAGGATTGCGATCAGCAACCAATTTAATTTT  >  USA300TCH1516_ALE/1789029‑1789309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: