Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,880,641 C→A 6.0% A1526A (GCG→GCT ebh_2 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,880,6410CA 6.0% 510.4 / 12.4 149A1526A (GCG→GCTebh_2Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base C (71/69);  new base A (6/3);  total (77/72)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 150 total aligned reads displayed.

TAGTGTAGCAATGCCATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTG  >  USA300TCH1516_ALE/1880503‑1880780
                                                                                                                                          |                                                                                                                                           
tAGTGTAGCAATGCCATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGc                                                                                                                                           <  3:171938/141‑1 (MQ=255)
 attgtAGCAATGCCATTTTTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTTTTTTAAAGCATCTTGTTTTTCTTTATCAGTCGCATGTTCCGCt                                                                                                                                          <  4:293458/139‑1 (MQ=255)
  gtgtAGCAATGCCATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAATCAATTTGTTTTTCTTCATCAGTCGCATGTTCCGCtt                                                                                                                                         >  1:706670/1‑141 (MQ=255)
   tgtAGCAATGCCATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTg                                                                                                                                        >  4:375590/1‑141 (MQ=255)
   tgtAGCAATGCCATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTGCATCAGTCGCATGTTCCGCTTg                                                                                                                                        <  3:173345/141‑1 (MQ=255)
   tgtAGCAATGCCATTTATTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCCATGTTTCGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTATTTTTTCATCAGTCGCATGTTCCGCttc                                                                                                                                        >  4:68686/1‑140 (MQ=255)
     tAGCAATGCCATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCt                                                                                                                                      <  3:383687/141‑1 (MQ=255)
     tAGCAATGCCATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCt                                                                                                                                      >  1:421282/1‑141 (MQ=255)
       gCAATGCCATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCa                                                                                                                                    <  2:147389/141‑1 (MQ=255)
         aaTGCCATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAAt                                                                                                                                  <  1:353854/141‑1 (MQ=255)
          aTGCCATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGTTCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAAtt                                                                                                                                 <  2:368872/141‑1 (MQ=255)
             ccATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTcg                                                                                                                              >  1:125010/1‑141 (MQ=255)
             ccATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTcg                                                                                                                              <  1:533616/141‑1 (MQ=255)
               aTTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTcgcg                                                                                                                            <  2:111363/141‑1 (MQ=255)
               aTTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTcgcg                                                                                                                            <  1:587691/141‑1 (MQ=255)
               aTTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTcgcg                                                                                                                            <  1:389704/141‑1 (MQ=255)
                tttGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCgcgc                                                                                                                           >  3:77994/1‑141 (MQ=255)
                tttGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCgcgc                                                                                                                           >  1:308242/1‑141 (MQ=255)
                 ttGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCt                                                                                                                          <  1:887249/141‑1 (MQ=255)
                 ttGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCAGCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCt                                                                                                                          <  1:135666/141‑1 (MQ=255)
                  tGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTGGCTCAATTTCGCGCtt                                                                                                                         >  3:298063/1‑141 (MQ=255)
                        aaCACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCATTTTCGCGCTTTTTAGc                                                                                                                   >  4:221913/1‑141 (MQ=255)
                        aaCACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGc                                                                                                                   >  2:700881/1‑141 (MQ=255)
                          cacGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCATTTTCGCGCTTTTTAGCtt                                                                                                                 >  2:56047/1‑141 (MQ=255)
                           acGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTc                                                                                                                <  1:841723/141‑1 (MQ=255)
                            cGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCa                                                                                                               <  2:337175/141‑1 (MQ=255)
                             gTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCat                                                                                                              >  2:197088/1‑141 (MQ=255)
                              tttCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCata                                                                                                             >  1:393618/1‑141 (MQ=255)
                                 cacaTCATGATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTTTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttt                                                                                                          >  4:11291/1‑140 (MQ=255)
                                  acaTCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgt                                                                                                         <  3:248864/141‑1 (MQ=255)
                                  acaTCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgt                                                                                                         >  2:608253/1‑141 (MQ=255)
                                  acaTCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgt                                                                                                         <  2:903915/141‑1 (MQ=255)
                                    atcatTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgttg                                                                                                       <  1:67191/141‑1 (MQ=255)
                                    atcatTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgttg                                                                                                       <  1:1043791/141‑1 (MQ=255)
                                       attattCGCTGTGGGTTGGTCTATGTTTTGTAATGCACGTTTTTCATTATTCTCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCATTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgtgtctt                                                                                                    >  2:222451/1‑136 (MQ=255)
                                       attattCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCTTTTTTTTTTTATTGATTTAAAGCATCTTGTTTTTTTTCATCAGTCGCATGTTCCGCTTGCTCTATTTCGCGCTTTTTAGCTTCATATTGCTGCtt                                                                                                    >  2:532615/1‑141 (MQ=255)
                                           ttCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgtt                                                                                                        >  3:142168/1‑133 (MQ=255)
                                           ttCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgtt                                                                                                        <  4:142168/133‑1 (MQ=255)
                                            tCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTg                                                                                               <  1:986575/141‑1 (MQ=255)
                                            tCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTg                                                                                               <  2:826172/141‑1 (MQ=255)
                                             cGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGc                                                                                              >  2:610558/1‑141 (MQ=255)
                                             cGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGc                                                                                              >  1:1048444/1‑141 (MQ=255)
                                               cTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCAt                                                                                            >  3:365137/1‑141 (MQ=255)
                                               cTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCAt                                                                                            <  4:442547/141‑1 (MQ=255)
                                                 aTTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCAtct                                                                                          >  2:392132/1‑141 (MQ=255)
                                                       tGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTagca                                                                                    >  1:1014219/1‑141 (MQ=255)
                                                       tGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTagca                                                                                    >  4:137927/1‑141 (MQ=255)
                                                         aTCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTagcagc                                                                                  <  1:1002883/141‑1 (MQ=255)
                                                            gATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgc                                                                               <  1:591143/141‑1 (MQ=255)
                                                            gATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgc                                                                               <  3:322999/141‑1 (MQ=255)
                                                             aTGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgct                                                                              <  2:57089/141‑1 (MQ=255)
                                                             aTGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgct                                                                              <  4:449292/141‑1 (MQ=255)
                                                              tGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGctc                                                                             <  4:77994/141‑1 (MQ=255)
                                                              tGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGctc                                                                             >  1:351674/1‑141 (MQ=255)
                                                              tGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGctc                                                                             >  1:137034/1‑141 (MQ=255)
                                                               gTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGctct                                                                            <  2:259944/141‑1 (MQ=255)
                                                                   tGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACa                                                                        <  2:671002/141‑1 (MQ=255)
                                                                    gTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACaa                                                                       <  2:63437/141‑1 (MQ=255)
                                                                     tAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAat                                                                      <  2:885875/141‑1 (MQ=255)
                                                                        tGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATg                                                                   <  2:966987/141‑1 (MQ=255)
                                                                         gCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATgg                                                                  >  1:624798/1‑141 (MQ=255)
                                                                         gCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATgg                                                                  >  1:624967/1‑141 (MQ=255)
                                                                         gCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATgg                                                                  >  3:197752/1‑141 (MQ=255)
                                                                               ttttCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGc                                                            >  1:770762/1‑141 (MQ=255)
                                                                                    attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCTTCTTTCGCATGTTCCGCTTGCTAAATTTCTCGCTTTTTAGCTTCATATTTTTTCTTAACTGCATCTCTAGCACCTGCTCTAATAATATGTTCTGGCGTCAc                                                       >  2:30778/1‑141 (MQ=255)
                                                                                    attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATCGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCAc                                                       >  1:646588/1‑141 (MQ=255)
                                                                                    attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCgctac                                                       >  4:302775/1‑137 (MQ=255)
                                                                                    attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCgctac                                                       >  1:266696/1‑137 (MQ=255)
                                                                                       tttttttAATTGATTTAAAGCAACTTGTTTTTTTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCTCGCTTTTTAGCTTTATATTGTTTCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATTGTCTGGCGTCACTaa                                                    >  4:307760/7‑141 (MQ=255)
                                                                                        ttCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTaaa                                                   <  3:264628/141‑1 (MQ=255)
                                                                                          cGCTAATTGATTTAAAGCAACTTGTTTTTTTTTTTTTTTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCTTCTCTAGCTTCTGCTCTTTCAATATGGTCTGTCGTCACTAAAGc                                                 >  2:638967/1‑141 (MQ=255)
                                                                                          cGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGc                                                 <  2:52810/141‑1 (MQ=255)
                                                                                             tAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAAt                                              >  2:750399/1‑141 (MQ=255)
                                                                                              aaTTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATg                                             <  2:707457/141‑1 (MQ=255)
                                                                                                  gATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCGCTACTAAAGCAATGCTAt                                         <  3:341344/141‑1 (MQ=255)
                                                                                                    tttAAAGCAACATGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCa                                       <  4:278078/141‑1 (MQ=255)
                                                                                                        aaGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAgcg                                   <  3:48522/141‑1 (MQ=255)
                                                                                                             aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTTATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                              >  2:907367/1‑141 (MQ=255)
                                                                                                             aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                              >  1:461685/1‑141 (MQ=255)
                                                                                                             aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                              <  1:700881/141‑1 (MQ=255)
                                                                                                             aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                              >  4:363787/1‑141 (MQ=255)
                                                                                                             aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                              >  4:397000/1‑141 (MQ=255)
                                                                                                             aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                              >  3:152818/1‑141 (MQ=255)
                                                                                                             aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                              >  2:235908/1‑141 (MQ=255)
                                                                                                              cTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCGCTACTAAAGCAATGCTATCAAGCGCTTGAc                             >  3:429216/1‑141 (MQ=255)
                                                                                                                tGTTTTTCTTCATCAGGCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACtt                           >  4:204606/1‑141 (MQ=255)
                                                                                                                  tttttCTTCATCAGTCGCATGTTCCGCTTGCTCGATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACttgt                         <  2:369565/141‑1 (MQ=255)
                                                                                                                  tttttCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACttgt                         <  1:983296/141‑1 (MQ=255)
                                                                                                                        ttcATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTatc                   >  2:1071744/1‑141 (MQ=255)
                                                                                                                         tcatcaGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTatca                  >  2:386593/1‑141 (MQ=255)
                                                                                                                         tcatcaGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTatca                  <  1:755843/141‑1 (MQ=255)
                                                                                                                            tcaGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTGCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTAtcatca               >  1:1062966/1‑141 (MQ=255)
                                                                                                                                tCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACtt           >  1:531389/1‑141 (MQ=255)
                                                                                                                                 cGCATGTTCCGCTTGCCCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCACCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCCAGCGCTTGACTTGTTGTACCCTCAACctg          >  2:688455/1‑141 (MQ=255)
                                                                                                                                  gCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgt         <  2:132540/141‑1 (MQ=255)
                                                                                                                                   cATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgtt        <  3:399199/141‑1 (MQ=255)
                                                                                                                                   cATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgtt        <  4:362767/141‑1 (MQ=255)
                                                                                                                                     tGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACACTATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGa      >  3:335211/1‑141 (MQ=255)
                                                                                                                                      gTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGAt     <  2:736455/141‑1 (MQ=255)
                                                                                                                                         ccGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTg  >  2:979980/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TAGTGTAGCAATGCCATTTGTTTCAACACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTG  >  USA300TCH1516_ALE/1880503‑1880780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: