BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	110,651	C→T	9.2%	D170D (GAC→GAT)	USA300HOU_RS00515 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	110,651	0	C	T	9.2%	416.9 / 22.4	149	D170D (GAC→GAT)	USA300HOU_RS00515	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base C (73/62); new base T (7/7); total (80/69)
Fisher's exact test for biased strand distribution p-value = 7.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 150 total aligned reads displayed.

AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGT  >  USA300TCH1516_ALE/110511‑110789

aaaaGATATCCGGTTAAAATGGGCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGAGTTTAAAAATTTGAAAAATTATAAAGAc                                                                                                                                             >  4:102041/1‑141 (MQ=255)

 aaaGATATCCGGTTAAAATGGAAGAAAAAAAAATCAAAAAAACAAAAGAAATTAAAAAAAAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACg                                                                                                                                            <  2:360694/141‑1 (MQ=18)

   aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGga                                                                                                                                          >  2:637828/1‑141 (MQ=255)

    gATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgag                                                                                                                                         >  3:238465/1‑141 (MQ=255)

     atatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgaga                                                                                                                                        >  1:347514/1‑141 (MQ=255)

         ccGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGAAGATAtt                                                                                                                                    >  2:940507/1‑141 (MQ=255)

           ggTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGAATTTAAAAATTTGAAAAATTATAAAAACGGAGAAATTTc                                                                                                                                  >  2:523062/1‑141 (MQ=255)

            gTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCa                                                                                                                                 >  1:864032/1‑141 (MQ=255)

                aaaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGTAGATATTTCAtata                                                                                                                             >  4:217948/1‑141 (MQ=255)

                     gTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCa                                                                                                                        >  2:403747/1‑141 (MQ=255)

                     gTCGATAATAAAATCAATCCAACAAAAAAAATTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCa                                                                                                                        <  4:58222/141‑1 (MQ=255)

                     agaaataataAAATCAAACCAACAAAAAAAATAAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCa                                                                                                                        <  4:277884/137‑1 (MQ=17)

                       cGATAATAAAATCATTCCAACAAAAGAAATTAAATATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                      <  1:47680/141‑1 (MQ=255)

                       cGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                      <  3:53036/141‑1 (MQ=255)

                           aataaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTg                                                                                                                  <  1:426927/141‑1 (MQ=255)

                             taaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                >  3:74776/1‑141 (MQ=255)

                             acaaaTCAGACCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATATGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                <  2:856610/139‑1 (MQ=255)

                               aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                              >  3:83937/1‑141 (MQ=255)

                               aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                              >  4:298870/1‑141 (MQ=255)

                               aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                              <  2:162656/141‑1 (MQ=255)

                               aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                              >  1:882023/1‑141 (MQ=255)

                                    aTTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTAt                                                                                                        <  3:48190/141‑1 (MQ=255)

                                       ccAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCg                                                                                                     >  1:123587/1‑141 (MQ=255)

                                         aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGATCTTTGAACAATATGGCGACTTTAAAAATTTGAAAAAATATAAAGACGAAGATAATTCATATAATACAGAGGGGACGAG‑TGATTctac                                                                                                   >  2:1078897/1‑138 (MQ=17)

                                           caaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTa                                                                                                 <  3:116205/141‑1 (MQ=255)

                                               aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAata                                                                                             <  2:336476/141‑1 (MQ=255)

                                               aGAAATTAAAGAAGAAAAAAAAAAAAAAAGAATCGAAAAATTTAAGTTTTTTGTTCAATATTGCGAATTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTTATTCGGCTAAat                                                                                              <  4:362279/141‑1 (MQ=17)

                                                  aaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCa                                                                                          >  2:921279/1‑141 (MQ=255)

                                                  aaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCa                                                                                          <  1:710481/141‑1 (MQ=255)

                                                     tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAAtt                                                                                       <  2:98220/141‑1 (MQ=255)

                                                     tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAAtt                                                                                       >  1:123531/1‑141 (MQ=255)

                                                     tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAAtt                                                                                       >  2:68829/1‑141 (MQ=255)

                                                      aaaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTa                                                                                      >  2:78679/1‑141 (MQ=255)

                                                        aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAAc                                                                                    >  3:415769/1‑141 (MQ=255)

                                                              aaaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAatga                                                                              <  2:292505/141‑1 (MQ=255)

                                                              aaaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAatga                                                                              >  3:326691/1‑141 (MQ=255)

                                                               aaaaTAAAAAAAGAAATCGAAAACTCTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAatgat                                                                             >  2:684640/1‑141 (MQ=255)

                                                                  aTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATgatgat                                                                          <  1:170259/141‑1 (MQ=255)

                                                                   tAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGAtt                                                                         >  1:873458/1‑141 (MQ=255)

                                                                    aaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                        <  1:75035/141‑1 (MQ=255)

                                                                     aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                       >  1:451867/1‑141 (MQ=255)

                                                                       aaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATaa                                                                     >  4:411443/1‑141 (MQ=255)

                                                                           gAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTa                                                                 <  1:228108/141‑1 (MQ=255)

                                                                               tCGAAAACTTTAAGTTCTTTGTACAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCAGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAc                                                             >  4:266937/1‑141 (MQ=255)

                                                                                cGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                            >  2:513448/1‑141 (MQ=255)

                                                                                 gAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAaaaaaa                                                           <  2:123587/141‑1 (MQ=255)

                                                                                 gAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACaa                                                           >  3:97353/1‑141 (MQ=255)

                                                                                     aCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                       >  1:241497/1‑141 (MQ=255)

                                                                                       tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                     <  2:964959/141‑1 (MQ=255)

                                                                                       tttAAGTTCTTTGTGCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                     <  1:568398/141‑1 (MQ=255)

                                                                                        ttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                    <  3:195689/141‑1 (MQ=255)

                                                                                        ttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGAGCCGAG‑TTATTCGGCTAAATATGAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                    >  3:321853/1‑141 (MQ=255)

                                                                                          aaGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                  >  3:143894/1‑141 (MQ=255)

                                                                                           aGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaaa                                                 >  2:297462/1‑141 (MQ=255)

                                                                                           aGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaaa                                                 >  1:932935/1‑141 (MQ=255)

                                                                                            gTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAg                                                <  3:2228/141‑1 (MQ=255)

                                                                                                  tGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatga                                          <  4:289477/141‑1 (MQ=255)

                                                                                                    ttCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                        <  3:387780/141‑1 (MQ=255)

                                                                                                     tCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgatat                                       >  1:192504/1‑141 (MQ=255)

                                                                                                      cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                                      <  2:877006/141‑1 (MQ=255)

                                                                                                      cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                                      >  3:376107/1‑141 (MQ=255)

                                                                                                      cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                                      >  2:671424/1‑141 (MQ=255)

                                                                                                        atatGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAcc                                    <  2:968273/141‑1 (MQ=255)

                                                                                                         tatGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAcc                                    <  2:906511/140‑1 (MQ=255)

                                                                                                         tatGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAcc                                    >  1:906511/1‑140 (MQ=255)

                                                                                                             gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAGTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                               >  4:437589/1‑141 (MQ=255)

                                                                                                             gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                               <  1:447574/141‑1 (MQ=255)

                                                                                                               gACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGt                             >  1:876126/1‑141 (MQ=255)

                                                                                                                 cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                           <  1:798377/141‑1 (MQ=255)

                                                                                                                 cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                           <  2:250708/141‑1 (MQ=255)

                                                                                                                 cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                           >  4:355963/1‑141 (MQ=255)

                                                                                                                        aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCa                    <  2:806287/141‑1 (MQ=255)

                                                                                                                          tttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                  <  4:21792/141‑1 (MQ=255)

                                                                                                                            tGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAGACACGAGTAAAGCTCCAAAGt                >  1:826540/1‑141 (MQ=255)

                                                                                                                             gAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt               <  3:119020/141‑1 (MQ=255)

                                                                                                                             aatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt               >  4:50053/5‑141 (MQ=255)

                                                                                                                              atgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa              <  2:297027/138‑1 (MQ=255)

                                                                                                                              atgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa              <  2:1055603/138‑1 (MQ=255)

                                                                                                                              atgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa              <  3:29057/138‑1 (MQ=255)

                                                                                                                               tgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt             >  1:770865/3‑141 (MQ=255)

                                                                                                                               aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt             <  4:179289/141‑1 (MQ=255)

                                                                                                                                aaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAtt            >  2:576138/1‑141 (MQ=255)

                                                                                                                                aaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAtt            >  1:458095/1‑141 (MQ=255)

                                                                                                                                  aTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt          >  3:30162/1‑141 (MQ=255)

                                                                                                                                  aTTATAAAGACGGAGATATTTCATATAAACCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGGAAAGCTCCAAAGTTATTGt          <  3:421563/141‑1 (MQ=255)

                                                                                                                                   ttATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtt         <  4:145481/141‑1 (MQ=255)

                                                                                                                                    tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTa        <  2:136780/141‑1 (MQ=255)

                                                                                                                                    tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCGCCAAAGTTATTGTTa        >  1:901596/1‑141 (MQ=255)

                                                                                                                                     ataAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa       <  3:171481/141‑1 (MQ=255)

                                                                                                                                      taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa      >  1:641364/1‑141 (MQ=255)

                                                                                                                                      taAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa      <  1:34510/141‑1 (MQ=255)

                                                                                                                                      taAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa      <  2:451867/141‑1 (MQ=255)

                                                                                                                                      taAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa      <  3:439940/141‑1 (MQ=255)

                                                                                                                                       aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaaa     >  1:411210/1‑141 (MQ=255)

                                                                                                                                       aaaGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGATaaaa     >  2:1034999/1‑141 (MQ=255)

                                                                                                                                         aGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAgg   >  3:361780/1‑141 (MQ=255)

                                                                                                                                         aGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAgg   <  1:627628/141‑1 (MQ=255)

                                                                                                                                         aGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAgg   <  2:604755/141‑1 (MQ=255)

                                                                                                                                         aGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACGAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAgg   <  1:510714/141‑1 (MQ=255)

                                                                                                                                          gACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt  >  3:154881/1‑141 (MQ=255)

AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAG‑TTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGT  >  USA300TCH1516_ALE/110511‑110789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`