Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,880,668 T→C 5.5% E1517E (GAA→GAG ebh_2 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,880,6680TC 5.5% 431.7 / 11.4 146E1517E (GAA→GAGebh_2Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base T (70/67);  new base C (6/2);  total (76/70)
Fisher's exact test for biased strand distribution p-value = 2.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

Only 100 of 148 total aligned reads displayed.

CACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGC  >  USA300TCH1516_ALE/1880529‑1880808
                                                                                                                                           |                                                                                                                                            
 acGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTc                                                                                                                                            <  1:841723/141‑1 (MQ=255)
   gTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCat                                                                                                                                          >  2:197088/1‑141 (MQ=255)
    tttCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCata                                                                                                                                         >  1:393618/1‑141 (MQ=255)
       cacaTCATGATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTTTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttt                                                                                                                                      >  4:11291/1‑140 (MQ=255)
        acaTCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgt                                                                                                                                     <  2:903915/141‑1 (MQ=255)
        acaTCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgt                                                                                                                                     >  2:608253/1‑141 (MQ=255)
        acaTCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgt                                                                                                                                     <  3:248864/141‑1 (MQ=255)
          atcatTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgttg                                                                                                                                   <  1:67191/141‑1 (MQ=255)
             attattCGCTGTGGGTTGGTCTATGTTTTGTAATGCACGTTTTTCATTATTCTCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCATTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgtgtctt                                                                                                                                >  2:222451/1‑136 (MQ=255)
             attattCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCTTTTTTTTTTTATTGATTTAAAGCATCTTGTTTTTTTTCATCAGTCGCATGTTCCGCTTGCTCTATTTCGCGCTTTTTAGCTTCATATTGCTGCtt                                                                                                                                >  2:532615/1‑141 (MQ=255)
                 ttCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgtt                                                                                                                                    <  4:142168/133‑1 (MQ=255)
                 ttCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATAttgtt                                                                                                                                    >  3:142168/1‑133 (MQ=255)
                  tCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTg                                                                                                                           <  1:986575/141‑1 (MQ=255)
                  tCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTg                                                                                                                           <  2:826172/141‑1 (MQ=255)
                   cGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGc                                                                                                                          >  2:610558/1‑141 (MQ=255)
                   cGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGc                                                                                                                          >  1:1048444/1‑141 (MQ=255)
                     cTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCAt                                                                                                                        <  4:442547/141‑1 (MQ=255)
                       aTTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCAtct                                                                                                                      >  2:392132/1‑141 (MQ=255)
                           cTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTag                                                                                                                  <  2:113120/141‑1 (MQ=255)
                             tGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTagca                                                                                                                >  1:1014219/1‑141 (MQ=255)
                             tGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTagca                                                                                                                >  4:137927/1‑141 (MQ=255)
                             tGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTagca                                                                                                                >  3:317975/1‑141 (MQ=255)
                               aTCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTagcagc                                                                                                              <  1:1002883/141‑1 (MQ=255)
                                  gATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgc                                                                                                           <  1:591143/141‑1 (MQ=255)
                                  gATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgc                                                                                                           <  3:322999/141‑1 (MQ=255)
                                   aTGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgct                                                                                                          <  4:449295/141‑1 (MQ=255)
                                   aTGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAgctgct                                                                                                          <  2:57089/141‑1 (MQ=255)
                                    tGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGctc                                                                                                         >  3:97959/1‑141 (MQ=255)
                                    tGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGctc                                                                                                         >  1:137034/1‑141 (MQ=255)
                                    tGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGctc                                                                                                         <  4:77994/141‑1 (MQ=255)
                                     gTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGctct                                                                                                        <  2:259944/141‑1 (MQ=255)
                                         tGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACa                                                                                                    <  2:671002/141‑1 (MQ=255)
                                          gTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACaa                                                                                                   <  2:63437/141‑1 (MQ=255)
                                           tAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAat                                                                                                  <  2:885875/141‑1 (MQ=255)
                                              tGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATg                                                                                               <  2:966987/141‑1 (MQ=255)
                                               gcaagTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATgt                                                                                              >  2:347684/5‑140 (MQ=255)
                                               gCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATgg                                                                                              >  3:197752/1‑141 (MQ=255)
                                               gCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATgg                                                                                              >  1:624798/1‑141 (MQ=255)
                                               gCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATgg                                                                                              >  1:624967/1‑141 (MQ=255)
                                                 aCGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTc                                                                                            <  4:2737/141‑1 (MQ=255)
                                                     ttttCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGc                                                                                        >  1:770762/1‑141 (MQ=255)
                                                     ttttCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGc                                                                                        >  1:48414/1‑141 (MQ=255)
                                                          attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCTTCTTTCGCATGTTCCGCTTGCTAAATTTCTCGCTTTTTAGCTTCATATTTTTTCTTAACTGCATCTCTAGCACCTGCTCTAATAATATGTTCTGGCGTCAc                                                                                   >  2:30778/1‑141 (MQ=255)
                                                          attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATCGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCAc                                                                                   >  1:646588/1‑141 (MQ=255)
                                                          attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCgctac                                                                                   >  1:266696/1‑137 (MQ=255)
                                                          attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCgctac                                                                                   >  4:417665/1‑137 (MQ=255)
                                                            tattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTa                                                                                 >  1:599773/1‑141 (MQ=255)
                                                             tttttttAATTGATTTAAAGCAACTTGTTTTTTTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCTCGCTTTTTAGCTTTATATTGTTTCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATTGTCTGGCGTCACTaa                                                                                >  4:307760/7‑141 (MQ=255)
                                                              ttCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTaaa                                                                               <  3:264628/141‑1 (MQ=255)
                                                                cGCTAATTGATTTAAAGCAACTTGTTTTTTTTTTTTTTTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCTTCTCTAGCTTCTGCTCTTTCAATATGGTCTGTCGTCACTAAAGc                                                                             >  2:638967/1‑141 (MQ=255)
                                                                cGCTAATTGATTTAAAGCAACTTGTTTTTTTTCTTTTTTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCGCTACTAAAGc                                                                             >  2:553394/1‑141 (MQ=255)
                                                                  cTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCaa                                                                           <  3:169884/141‑1 (MQ=255)
                                                                  cTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCaa                                                                           <  3:137927/141‑1 (MQ=255)
                                                                   tAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAAt                                                                          >  2:750399/1‑141 (MQ=255)
                                                                    aaTTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATg                                                                         <  2:707457/141‑1 (MQ=255)
                                                                        gATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCGCTACTAAAGCAATGCTAt                                                                     <  4:364864/141‑1 (MQ=255)
                                                                        gATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCGCTACTAAAGCAATGCTAt                                                                     <  3:341344/141‑1 (MQ=255)
                                                                          tttAAAGCAACATGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCa                                                                   <  4:278078/141‑1 (MQ=255)
                                                                                gCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCt                                                             <  1:1051728/141‑1 (MQ=255)
                                                                                  aaCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTg                                                           <  2:699913/141‑1 (MQ=255)
                                                                                   aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTTATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                                          >  2:907367/1‑141 (MQ=255)
                                                                                   aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                                          <  2:1027333/141‑1 (MQ=255)
                                                                                   aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                                          <  1:700881/141‑1 (MQ=255)
                                                                                   aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                                          >  1:388911/1‑141 (MQ=255)
                                                                                   aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                                          >  2:235908/1‑141 (MQ=255)
                                                                                   aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                                          >  4:363787/1‑141 (MQ=255)
                                                                                   aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                                          >  3:418040/1‑141 (MQ=255)
                                                                                    cTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCGCTACTAAAGCAATGCTATCAAGCGCTTGAc                                                         >  3:429216/1‑141 (MQ=255)
                                                                                      tGTTTTTCTTCATCAGGCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACtt                                                       >  4:204606/1‑141 (MQ=255)
                                                                                       gTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACttg                                                      >  1:68614/1‑141 (MQ=255)
                                                                                        tttttCTTCATCAGTCGCATGTTCCGCTTGCTCGATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACttgt                                                     <  2:369565/141‑1 (MQ=255)
                                                                                        tttttCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACttgt                                                     <  1:983296/141‑1 (MQ=255)
                                                                                              ttcATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTatc                                               >  2:1071744/1‑141 (MQ=255)
                                                                                               tcatcaGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTatca                                              <  1:755843/141‑1 (MQ=255)
                                                                                                      tCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACtt                                       >  1:531389/1‑141 (MQ=255)
                                                                                                       cGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAAcaag                                      <  1:755526/141‑4 (MQ=255)
                                                                                                        gCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgt                                     <  2:132540/141‑1 (MQ=255)
                                                                                                         cATGTTCCGCTCGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgtt                                    <  3:365245/141‑1 (MQ=255)
                                                                                                          aTGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgttg                                   <  3:339573/141‑1 (MQ=255)
                                                                                                            gTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGAt                                 <  2:736455/141‑1 (MQ=255)
                                                                                                                 gCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGtt                            <  1:13481/141‑1 (MQ=255)
                                                                                                                 gCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGtt                            <  4:328526/141‑1 (MQ=255)
                                                                                                                  cTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAAGATGGTCAGGCGTCACGAAAGCAATGCTAGCAAGCGCGTGACTTGTTGTATCATCAACTGGTTGATTTGTTc                           >  1:103229/1‑141 (MQ=255)
                                                                                                                     gCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtat                        <  1:834011/141‑1 (MQ=255)
                                                                                                                     gCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtat                        >  3:391697/1‑141 (MQ=255)
                                                                                                                      cTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtatt                       <  2:316563/141‑1 (MQ=255)
                                                                                                                        cAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtattat                     >  3:26570/1‑141 (MQ=255)
                                                                                                                        cAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtattat                     <  3:162647/141‑1 (MQ=255)
                                                                                                                        cAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtattat                     >  2:393019/1‑141 (MQ=255)
                                                                                                                         aaTTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTattatt                    <  2:46876/141‑1 (MQ=255)
                                                                                                                          aTTTCGCGCTTTTTAGCTTCATATTGTGGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATTGTCATTCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTc                   >  1:702942/1‑141 (MQ=255)
                                                                                                                                cgcTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAata             >  1:649408/1‑141 (MQ=255)
                                                                                                                                   tttttAGCGTCATTTTGTTGCTTAATTTCATCTCTATCAGCTGCTCTATCGAGATGATCTGTCGTCTCTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCt          <  2:683015/141‑1 (MQ=255)
                                                                                                                                    ttttAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTg         <  1:553955/141‑1 (MQ=255)
                                                                                                                                      ttAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTc       >  2:964200/1‑141 (MQ=255)
                                                                                                                                        aGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCAt     >  1:212231/1‑141 (MQ=255)
                                                                                                                                        aGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCAt     >  4:170061/1‑141 (MQ=255)
                                                                                                                                        aGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCAt     >  1:564915/1‑141 (MQ=255)
                                                                                                                                        aGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCAt     >  2:460270/1‑141 (MQ=255)
                                                                                                                                           ttCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGc  >  3:291294/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CACGTTTCACATCATTATTCGCTATTGCTTGATCGATGTTTTGTAATGCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGC  >  USA300TCH1516_ALE/1880529‑1880808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: