Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,005,544 G→A 5.1% S40S (AGC→AGT)  ghrB_2 ← Glyoxylate/hydroxypyruvate reductase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,005,5440GA 5.1% 420.9 / 7.0 135S40S (AGC→AGT) ghrB_2Glyoxylate/hydroxypyruvate reductase B
Reads supporting (aligned to +/- strand):  ref base G (51/77);  new base A (3/4);  total (54/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 136 total aligned reads displayed.

TGATCTGCAATATAATCTAACGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGTTTTTAAGTT  >  USA300TCH1516_ALE/2005404‑2005682
                                                                                                                                            |                                                                                                                                          
 gATCTGCAATATAATCTAACGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCTCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGc                                                                                                                                           >  1:223301/1‑141 (MQ=255)
    cTGCAATATAATCTAACGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGctct                                                                                                                                        <  1:17308/141‑1 (MQ=255)
        aaTATAATCTAACGGTAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTTGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCACTCTCAgg                                                                                                                                    <  2:874941/141‑1 (MQ=255)
        aaTATAATCTAACGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAgg                                                                                                                                    <  2:525939/141‑1 (MQ=255)
         atatAATCTAACGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGt                                                                                                                                   >  1:740467/1‑141 (MQ=255)
              aTCTAACGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTc                                                                                                                              >  1:1003405/1‑141 (MQ=255)
              aTCTAACGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTc                                                                                                                              >  1:529842/1‑141 (MQ=255)
              aTCTAACGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTc                                                                                                                              >  1:18268/1‑141 (MQ=255)
                cTAACGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTg                                                                                                                            >  3:183773/1‑141 (MQ=255)
                cTAACGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTATg                                                                                                                            >  2:301864/1‑141 (MQ=255)
                 tAACGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGa                                                                                                                           <  3:279795/141‑1 (MQ=255)
                 tAACGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGACATAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGa                                                                                                                           <  2:669915/141‑1 (MQ=255)
                   aCGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATg                                                                                                                         >  2:899670/1‑141 (MQ=255)
                      gCAATGTATTTACACCCGTTGAAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCtt                                                                                                                      >  1:526052/1‑141 (MQ=255)
                       cAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCttt                                                                                                                     <  4:240894/141‑1 (MQ=255)
                       cAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCttt                                                                                                                     <  3:307199/141‑1 (MQ=255)
                        aaTGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCtttt                                                                                                                    >  2:935572/1‑141 (MQ=255)
                          tGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCtttttt                                                                                                                  >  1:375373/1‑141 (MQ=255)
                          tGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCtttttt                                                                                                                  >  2:564091/1‑141 (MQ=255)
                            tATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTtat                                                                                                                <  3:48861/141‑1 (MQ=255)
                             agtgacacCCGTTGCAAACCATGCAATCCATTTTAACTTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCGGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTtata                                                                                                               <  1:288522/137‑1 (MQ=255)
                             aTTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTtata                                                                                                               <  1:947607/141‑1 (MQ=255)
                              tttACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATaa                                                                                                              >  2:527937/1‑141 (MQ=255)
                                tACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATaaaa                                                                                                            <  2:1002876/141‑1 (MQ=255)
                                 acacCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAAt                                                                                                           >  1:93338/1‑141 (MQ=255)
                                    cccGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTa                                                                                                        <  2:462379/141‑1 (MQ=255)
                                    cccGTTGCAAACCATACAATCCATTTTAAATTCGGGCAACGTTGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCACTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTa                                                                                                        <  1:903106/141‑1 (MQ=255)
                                      cGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTaaa                                                                                                      >  1:646090/1‑141 (MQ=255)
                                       gTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTaaaa                                                                                                     <  1:1080845/141‑1 (MQ=255)
                                          gCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAaaatct                                                                                                  >  2:783219/1‑141 (MQ=255)
                                           cAAACCATGCAATCCATTTTAAATTCGGGCTAAGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTATTATATCTAAGTCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAaaatata                                                                                                 <  4:130650/141‑1 (MQ=255)
                                            aaaCCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAaaatctaa                                                                                                <  3:285077/141‑1 (MQ=255)
                                              aCCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAaaatctaaat                                                                                              <  3:185874/141‑1 (MQ=255)
                                              aCCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAaaatctaaat                                                                                              >  1:619453/1‑141 (MQ=255)
                                              aCCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAaaatctaaat                                                                                              >  1:63727/1‑141 (MQ=255)
                                                 aTGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTg                                                                                           <  3:165955/141‑1 (MQ=255)
                                                 aTGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTg                                                                                           <  4:396317/141‑1 (MQ=255)
                                                  tGCAATCCATTTTCAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCTTAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGa                                                                                          <  2:149128/141‑1 (MQ=255)
                                                     aaTCCATTTTAAATTCGGGCAACGTTGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCACTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGaaaa                                                                                       >  1:664676/1‑141 (MQ=255)
                                                     aaTCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGaaaa                                                                                       >  2:466207/1‑141 (MQ=255)
                                                       tCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGc                                                                                     <  4:361867/141‑1 (MQ=255)
                                                               aaaTTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTtgttgt                                                                             <  2:425238/141‑1 (MQ=255)
                                                                  ttCGGGCAACGTCGTAAAGATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAAt                                                                          >  1:1071925/1‑141 (MQ=255)
                                                                  ttCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAAt                                                                          >  2:1029561/1‑141 (MQ=255)
                                                                   tCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAAtt                                                                         <  2:1051561/141‑1 (MQ=255)
                                                                    cGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTc                                                                        >  1:819414/1‑141 (MQ=255)
                                                                    cGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTc                                                                        >  1:993359/1‑141 (MQ=255)
                                                                     gggCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCa                                                                                                                                      <  2:771877/78‑1 (MQ=255)
                                                                     gggCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCa                                                                                                                                      >  1:771877/1‑78 (MQ=255)
                                                                       gCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTACTTCAGt                                                                     >  2:483264/1‑141 (MQ=255)
                                                                         aaCGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGttt                                                                   <  4:373087/141‑1 (MQ=255)
                                                                           cgtcgtAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCa                                                                 <  4:297814/141‑1 (MQ=255)
                                                                           cgtcgtAAAAAGGCCTCATTGATACCGCCATCATAACCAGCTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCa                                                                 <  2:93105/141‑1 (MQ=255)
                                                                             tcgtAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAAc                                                               <  2:973799/141‑1 (MQ=255)
                                                                               gtAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACtt                                                             >  1:184843/1‑141 (MQ=255)
                                                                               gtAAAAATGCCTCATTGATACCGCCAGCATACCCCACTAATATATCTAAATCAACCAAGTCGCTCTCAGGCATTTCTGATGCTTTTTTATACAATTCAAAATCTAAATCTGAAAAGCGTTGTTATATTCCAGGTTCAACtc                                                             >  2:741904/1‑140 (MQ=255)
                                                                                       gCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATa                                                     <  3:230105/141‑1 (MQ=255)
                                                                                       gCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATa                                                     <  1:1017120/141‑1 (MQ=255)
                                                                                        ccTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTTTAATTTAGGTTCAACTTCACGCATAc                                                    >  1:283473/1‑141 (MQ=255)
                                                                                        ccTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATAc                                                    >  3:281531/1‑141 (MQ=255)
                                                                                          tCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGa                                                  <  1:331284/141‑1 (MQ=255)
                                                                                                aTACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCACTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATGCAGTTTCAACTTCACGCATACGATTTAAc                                            >  1:983644/1‑141 (MQ=255)
                                                                                                   ccGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAAccct                                         <  1:1030917/141‑2 (MQ=255)
                                                                                                   ccGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCca                                         >  1:801908/1‑141 (MQ=255)
                                                                                                   ccGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCca                                         <  2:890586/141‑1 (MQ=255)
                                                                                                     gCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCcaac                                       <  2:801908/141‑1 (MQ=255)
                                                                                                      ccATCATAACCAACTAATATATCTAAAGCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCcaaca                                      <  1:593231/141‑1 (MQ=255)
                                                                                                       catcatAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCcaacaa                                     <  3:262277/141‑1 (MQ=255)
                                                                                                       catcatAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCcaacaa                                     <  3:147711/141‑1 (MQ=255)
                                                                                                         tcatAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACt                                   <  3:235136/141‑1 (MQ=255)
                                                                                                         tcatAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACt                                   >  1:261683/1‑141 (MQ=255)
                                                                                                          catAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACtt                                  <  2:130843/141‑1 (MQ=255)
                                                                                                            tAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAGAGCTTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTc                                <  2:432781/141‑1 (MQ=255)
                                                                                                             aaCCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCa                               <  1:1063876/141‑1 (MQ=255)
                                                                                                             aaCCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCa                               >  1:309211/1‑141 (MQ=255)
                                                                                                              aCCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCat                              >  1:794007/1‑141 (MQ=255)
                                                                                                               ccAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCata                             >  1:721814/1‑141 (MQ=255)
                                                                                                                 aaCTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCatata                           >  2:138035/1‑141 (MQ=255)
                                                                                                                   cTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATg                         >  2:34383/1‑141 (MQ=255)
                                                                                                                      atatatCTAAATCAGCCAAGTCACTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCa                      <  1:215250/141‑1 (MQ=255)
                                                                                                                         tatCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCAccc                   <  2:129599/141‑1 (MQ=255)
                                                                                                                         tatCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCAccc                   >  3:73381/1‑141 (MQ=255)
                                                                                                                          atCTAAATCAGCCGAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCAcccc                  <  2:299763/141‑1 (MQ=255)
                                                                                                                          atCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCAcccc                  <  4:122388/141‑1 (MQ=255)
                                                                                                                              aaaTCAGCCAAGTCGCTCTCAGGTATATCTGATGCTTTTTTATAAAATTTAAAAACTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCaaaa              <  2:861526/141‑1 (MQ=255)
                                                                                                                                aTCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGt                        >  3:103105/1‑129 (MQ=255)
                                                                                                                                aTCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGt                        <  4:103105/129‑1 (MQ=255)
                                                                                                                                  cAGCCAAGTCGCTCTCCAGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTCACAACTTCCCGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGtt          <  4:154736/141‑1 (MQ=255)
                                                                                                                                    cccAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGtttt        <  2:85107/140‑1 (MQ=255)
                                                                                                                                     ccAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTTTATTATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGttttt       >  2:23932/1‑141 (MQ=255)
                                                                                                                                     ccAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGttttt       <  2:134108/141‑1 (MQ=255)
                                                                                                                                     ccAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGttttt       >  1:904748/1‑141 (MQ=255)
                                                                                                                                     ccAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGttttt       <  2:412052/141‑1 (MQ=255)
                                                                                                                                     ccAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTGCATATATGTCACCCCAAAATGttttt       >  2:858102/1‑141 (MQ=255)
                                                                                                                                      cAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGTTTTTa      >  1:959133/1‑141 (MQ=255)
                                                                                                                                        aGTCGCTCACAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTACAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGTTTTTAAg    >  3:369507/1‑141 (MQ=255)
                                                                                                                                         gTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGTTTTTAAGt   <  2:1079776/141‑1 (MQ=255)
                                                                                                                                          tCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGTTTTTAAGtt  <  1:731132/141‑1 (MQ=255)
                                                                                                                                          tCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGTTTTTAAGtt  <  1:731221/141‑1 (MQ=255)
                                                                                                                                          tCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGTTTTTAAGtt  <  2:18268/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TGATCTGCAATATAATCTAACGGCAATGTATTTACACCCGTTGCAAACCATGCAATCCATTTTAAATTCGGGCAACGTCGTAAAAATGCCTCATTGATACCGCCATCATAACCAACTAATATATCTAAATCAGCCAAGTCGCTCTCAGGTATTTCTGATGCTTTTTTATAAAATTTAAAATCTAAATCTGAAAAGCGTTGTTGTAATTCAGTTTCAACTTCACGCATACGATTTAACCCAACAACTTTCATATATGTCACCCCAAAATGTTTTTAAGTT  >  USA300TCH1516_ALE/2005404‑2005682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑