Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,016,103 G→A 5.4% Y207Y (TAC→TAT tagH_2 ← Teichoic acids export ATP‑binding protein TagH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,016,1030GA 5.4% 410.0 / 8.0 128Y207Y (TAC→TATtagH_2Teichoic acids export ATP‑binding protein TagH
Reads supporting (aligned to +/- strand):  ref base G (68/53);  new base A (4/3);  total (72/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 128 total aligned reads displayed.

TTTTCCAATCTAAATCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGTCAATAACATGATTTA  >  USA300TCH1516_ALE/2015964‑2016239
                                                                                                                                           |                                                                                                                                        
ttttCCAATCTAAATCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGt                                                                                                                                         <  1:415448/141‑1 (MQ=255)
ttttCCAATCTAAATCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGCTCATGTTCTTGAAAATATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGt                                                                                                                                         >  3:42589/1‑141 (MQ=255)
 gttCCAAACGAAAGCAAAGTTTTCAATTTCTTCTTTTGTGATTAGACTTAAGCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTATTAAAAAAACATGTGAAAACAAAGCTATGTa                                                                                                                                        >  2:976784/2‑141 (MQ=255)
   tCCAATCTAAATCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGt                                                                                                                                      >  3:236598/1‑141 (MQ=255)
   tCCAATCTAAATCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGt                                                                                                                                      >  2:467897/1‑141 (MQ=255)
    cttttCTATATCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGtt                                                                                                                                     >  2:331506/5‑141 (MQ=255)
    ccAATCTAAATCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGtt                                                                                                                                     >  2:402094/1‑141 (MQ=255)
     cAATCTAAATCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTa                                                                                                                                    <  3:450424/141‑1 (MQ=255)
         ttttttCAAAGTTTTGAATTTCTTCTATTTAGTTTACACTTACTCGATCACGTTCATGTTCTTTAAAAGATGTTTTATTTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACAAAGCTATGTAGTTACTCa                                                                                                                                >  2:53872/6‑141 (MQ=255)
           aaaTCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCTCGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACt                                                                                                                              <  2:578182/141‑1 (MQ=255)
           aaaTCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACt                                                                                                                              <  4:454100/141‑1 (MQ=255)
           aaaTCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACt                                                                                                                              <  1:638347/141‑1 (MQ=255)
            aaTCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACtt                                                                                                                             >  3:145377/1‑141 (MQ=255)
             aTCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTtg                                                                                                                            <  3:363027/141‑1 (MQ=255)
              tCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTtgt                                                                                                                           <  3:128680/141‑1 (MQ=255)
               cAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTtgtg                                                                                                                          >  2:301943/1‑141 (MQ=255)
                 aaGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGaa                                                                                                                        <  4:178089/141‑1 (MQ=255)
                  aGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGaaa                                                                                                                       >  2:543902/1‑141 (MQ=255)
                   gTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAAt                                                                                                                      >  1:747676/1‑141 (MQ=255)
                    ttttCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAAtt                                                                                                                     >  2:175416/1‑141 (MQ=255)
                    ttttCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAAtt                                                                                                                     >  3:66223/1‑141 (MQ=255)
                     tttCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAAttt                                                                                                                    <  3:12599/141‑1 (MQ=255)
                      ttCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAAtttt                                                                                                                   <  2:778673/141‑1 (MQ=255)
                       tCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTa                                                                                                                  >  1:934856/1‑141 (MQ=255)
                       tCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTa                                                                                                                  >  2:609535/1‑141 (MQ=255)
                       tCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTa                                                                                                                  >  1:741948/1‑141 (MQ=255)
                       tCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTa                                                                                                                  >  1:1054354/1‑141 (MQ=255)
                       tCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTa                                                                                                                  <  1:616066/141‑1 (MQ=255)
                        cAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTAt                                                                                                                 <  1:252576/141‑1 (MQ=255)
                         aaTTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATc                                                                                                                >  1:437911/1‑141 (MQ=255)
                               tttttttTATTTTAGCCTTAATCGTTCACGTTCATGTTCTTTAAAAGAGGGAATAACTTTTTTAAGTGACCCTTCCATTCTTAATTTTTCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATatc                                                                                                          <  2:331546/138‑1 (MQ=255)
                               ttcttTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATatc                                                                                                          >  2:480367/1‑141 (MQ=255)
                               ttcttTAGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAGATTTTATCAATatc                                                                                                          >  4:374739/1‑141 (MQ=255)
                                tcttTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATatca                                                                                                         <  3:284150/141‑1 (MQ=255)
                                tcttTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATatca                                                                                                         <  4:308260/141‑1 (MQ=255)
                                   tttGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATAtcatca                                                                                                      >  2:736329/1‑141 (MQ=255)
                                    ttGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCAc                                                                                                     >  3:52017/1‑141 (MQ=255)
                                         tttAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAAtt                                                                                                <  1:463590/141‑1 (MQ=255)
                                            aGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGac                                                                                             >  1:280873/1‑141 (MQ=255)
                                             gACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGaca                                                                                            <  4:230403/141‑1 (MQ=255)
                                              aCTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGacac                                                                                           >  1:308859/1‑141 (MQ=255)
                                              aCTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGacac                                                                                           <  2:570899/141‑1 (MQ=255)
                                                  aaTCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATc                                                                                       >  2:836586/1‑141 (MQ=255)
                                                      gATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTa                                                                                   <  1:2020/141‑1 (MQ=255)
                                                       aTCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTGAATTGACCATTTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTaa                                                                                  >  1:702864/1‑141 (MQ=255)
                                                         cACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAat                                                                                <  2:217857/141‑1 (MQ=255)
                                                         cACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAat                                                                                >  2:217408/1‑141 (MQ=255)
                                                         cACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAat                                                                                <  1:87346/141‑1 (MQ=255)
                                                                   ttCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAat                                                                      >  1:95922/1‑141 (MQ=255)
                                                                      tttAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAAtata                                                                   <  3:397409/141‑1 (MQ=255)
                                                                         aaaaGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATc                                                                <  1:84108/141‑1 (MQ=255)
                                                                           aaGATGGAATATCTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCAt                                                              <  2:431546/141‑1 (MQ=255)
                                                                           aaGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCAt                                                              >  1:624337/1‑141 (MQ=255)
                                                                             gATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCAttt                                                            >  1:937166/1‑141 (MQ=255)
                                                                             gATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAAAGATGCTATATAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCAttt                                                            >  1:565856/1‑141 (MQ=255)
                                                                               tGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGt                                                          <  1:981561/141‑1 (MQ=255)
                                                                                ggAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGtt                                                         >  1:121504/1‑141 (MQ=255)
                                                                                         tGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATc                                                <  1:1021718/141‑1 (MQ=255)
                                                                                          gTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTCTCAATCTCCTAACCAATAGACACACTCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCg                                               >  1:99180/1‑141 (MQ=255)
                                                                                          gTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCg                                               >  2:409707/1‑141 (MQ=255)
                                                                                           tttAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTa                                                                                   >  1:1005021/1‑104 (MQ=255)
                                                                                           tttAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGc                                              >  2:1041286/1‑141 (MQ=255)
                                                                                           tttAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGc                                              >  3:222552/1‑141 (MQ=255)
                                                                                           tttAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGc                                              >  3:222531/1‑141 (MQ=255)
                                                                                           tttAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGc                                              >  2:285935/1‑141 (MQ=255)
                                                                                           tttAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGc                                              <  2:987308/141‑1 (MQ=255)
                                                                                               aGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGt                                          >  3:136734/1‑141 (MQ=255)
                                                                                                gTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGtt                                         >  4:393545/1‑141 (MQ=255)
                                                                                                   aCCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCa                                      <  2:1029522/141‑1 (MQ=255)
                                                                                                   aCCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCa                                      <  1:900852/141‑1 (MQ=255)
                                                                                                   aCCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCa                                      <  1:107978/141‑1 (MQ=255)
                                                                                                       ttCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATaaa                                  >  2:876845/1‑141 (MQ=255)
                                                                                                        tCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAAt                                 <  2:904723/141‑1 (MQ=255)
                                                                                                          cATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATtg                               <  1:1007740/141‑1 (MQ=255)
                                                                                                          cATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATtg                               <  1:282049/141‑1 (MQ=255)
                                                                                                              cTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTgtggt                           >  2:793282/1‑141 (MQ=255)
                                                                                                               ttAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTgtggtg                          <  2:521925/141‑1 (MQ=255)
                                                                                                                    tGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTaaa                     >  2:406026/1‑141 (MQ=255)
                                                                                                                    tGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGAGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTaaa                     >  1:573267/1‑141 (MQ=255)
                                                                                                                     gACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAAt                    >  1:586902/1‑141 (MQ=255)
                                                                                                                     gACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATAGCACGTTCCATAAATTGTGGTGTTAAAt                    >  2:874678/1‑141 (MQ=255)
                                                                                                                       ccATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAg                  >  4:338782/1‑141 (MQ=255)
                                                                                                                        cATGTGAAAACCATGCTATATAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTTTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTCAATAGt                 >  2:1038473/1‑141 (MQ=255)
                                                                                                                        cATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCCCGTTCCATAAATTGTGGTGTTAAATAGt                 >  4:236952/1‑141 (MQ=255)
                                                                                                                        cATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGt                 >  1:990851/1‑141 (MQ=255)
                                                                                                                          tgtgAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGTCa               <  3:348712/141‑1 (MQ=255)
                                                                                                                          tgtgAAAACCAAGCTATATAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAAt                                 <  1:35717/123‑1 (MQ=255)
                                                                                                                          tgtgAAAACCAAGCTATATAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAAt                                 >  2:35717/1‑123 (MQ=255)
                                                                                                                           gtgAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGTCaa              >  1:999280/1‑141 (MQ=255)
                                                                                                                           gtgAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGTCaa              <  2:299236/141‑1 (MQ=255)
                                                                                                                            tgAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGTCAAt             <  2:265019/141‑1 (MQ=255)
                                                                                                                               aaaCCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGTCAATAAc          >  3:393501/1‑141 (MQ=255)
                                                                                                                               aaaCCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGTCAATAAc          >  1:479232/1‑141 (MQ=255)
                                                                                                                                aaCCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGTCAATAACa         <  3:256915/141‑1 (MQ=255)
                                                                                                                                 aCCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGTCAATAACAt        >  2:645965/1‑141 (MQ=255)
                                                                                                                                  ccAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAACTGTGGTGTTAAATAGTCAATAACATg       >  2:860944/1‑141 (MQ=255)
                                                                                                                                    aaGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGTCAATAACATGAt     <  2:1006745/141‑1 (MQ=255)
                                                                                                                                    aaGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGTCAATAACATGAt     >  2:699262/1‑141 (MQ=255)
                                                                                                                                      gCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGTCAATAACATGAttt   >  2:696538/1‑141 (MQ=255)
                                                                                                                                       cTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCGCGTTCCATAAATTGTGGTGTTAAATAGTAAATAACATGATTTa  >  1:854453/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                        
TTTTCCAATCTAAATCAAAGTTTTCAATTTCTTCTTTTGAGTTTAGACTTAATCGATCACGTTCATGTTCTTTAAAAGATGGAATAACTTGTTTAAGTGACCCTTCCATTCTTAATTGACCATGTGAAAACCAAGCTATGTAGTTACTCACTTGTGAAATTTTATCAATATCATCACCAATTGACACAATCGTTAAATTATTTTCAATATAATCATTTGTTAATTCAATCGCACGTTCCATAAATTGTGGTGTTAAATAGTCAATAACATGATTTA  >  USA300TCH1516_ALE/2015964‑2016239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: