Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,176,264 A→G 5.1% intergenic (+26/+5269) ilvA → / ← USA300TCH1516_02069 L‑threonine dehydratase biosynthetic IlvA/tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,176,2640AG 5.1% 408.7 / 7.1 135intergenic (+26/+5269)ilvA/USA300TCH1516_02069L‑threonine dehydratase biosynthetic IlvA/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base A (71/56);  new base G (4/3);  total (75/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

ATTCAACTTAAAGATCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGACATAAAA  >  USA300TCH1516_ALE/2176125‑2176402
                                                                                                                                            |                                                                                                                                           
aTTCAACTTAAAGATCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAAt                                                                                                                                            >  2:110547/1‑141 (MQ=255)
 ttCAACTTAAAGATCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAAtt                                                                                                                                           >  3:145241/1‑141 (MQ=255)
      cTTAAAGATCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGAttt                                                                                                                                      <  2:171047/141‑1 (MQ=255)
             aTCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTg                                                                                                                              >  1:617276/1‑141 (MQ=255)
             aTCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTg                                                                                                                              >  3:100194/1‑141 (MQ=255)
                atgatgATTTAATACAACTCAAACAACGGGTAGAACATTTCGATGCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGaaa                                                                                                                           >  2:594736/1‑141 (MQ=255)
                  gatgatTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAGCAGTCATAAGATGATTTCAATTAGAAatc                                                                                                                         <  4:34317/141‑1 (MQ=38)
                    tgatTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAatcat                                                                                                                       >  3:309868/1‑141 (MQ=255)
                    tgatTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAatcat                                                                                                                       <  2:332190/141‑1 (MQ=255)
                     gatTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAatcatc                                                                                                                      >  3:406147/1‑141 (MQ=255)
                     gatTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAatcatc                                                                                                                      >  2:510057/1‑141 (MQ=255)
                     gatTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAatcatc                                                                                                                      >  2:416897/1‑141 (MQ=255)
                         tAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTGAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTAt                                                                                                                  >  1:853946/1‑141 (MQ=255)
                          aaTACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATg                                                                                                                 <  4:192681/141‑1 (MQ=255)
                          aaTACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATg                                                                                                                 <  1:637285/141‑1 (MQ=255)
                           aTACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAGTTGATTTATAATTGAAATCATCTTATGa                                                                                                                >  3:404528/1‑141 (MQ=255)
                           aTACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGa                                                                                                                >  1:932694/1‑141 (MQ=255)
                            caCAACTCCAACAACCTGTAAATCATTTCGATCCTTCCAATAATTATATTAATGAAAATAAGATGTGATATTCATTGTTAATTTAACACATACTAAGAACATCAGTCATAAATTGATTTCTAATTGAAATCATCTTATGAc                                                                                                               <  2:1019040/140‑1 (MQ=255)
                            atcgttacAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGAc                                                                                                               <  1:178671/134‑1 (MQ=255)
                              cAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAAGCAGTCATAAGATGATTTCAATTAGAAATCAATTTATGACt                                                                                                              <  3:418999/141‑1 (MQ=25)
                              cAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTg                                                                                                             >  4:61660/1‑141 (MQ=255)
                                aCTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCt                                                                                                           >  1:493904/1‑141 (MQ=255)
                                 cTCACACAACGTGTAAATCGTTACGATCCTTCCAATATTAATATAACAAAAACTAAGATGTTATAATCATAGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTACTAATTGACATAATCTAATgcaaactt                                                                                                          >  2:432112/1‑134 (MQ=255)
                                 cTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCtt                                                                                                          >  2:63177/1‑141 (MQ=255)
                                     aacaacGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTtta                                                                                                      >  3:443365/1‑141 (MQ=255)
                                       caacGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTttatt                                                                                                    >  2:327841/1‑141 (MQ=255)
                                        aacGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTttatta                                                                                                   >  2:482814/1‑141 (MQ=255)
                                         acGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTtattat                                                                                                  >  1:917604/1‑141 (MQ=255)
                                         acGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTtattat                                                                                                  >  4:145175/1‑141 (MQ=255)
                                         acGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTtattat                                                                                                  >  2:105053/1‑141 (MQ=255)
                                          cGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAGATGATTTCAATTAGAAATCAATTTATGACTGTttttcttacta                                                                                                 <  1:304750/141‑8 (MQ=21)
                                          cGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATtata                                                                                                 >  2:55904/1‑141 (MQ=255)
                                             gtAAATCGTTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACtt                                                                                              >  1:860097/1‑141 (MQ=255)
                                             gtAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACtt                                                                                              >  2:93627/1‑141 (MQ=255)
                                             gtAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACtt                                                                                              >  1:178711/1‑141 (MQ=255)
                                              tAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACttt                                                                                             <  2:1051845/141‑1 (MQ=255)
                                              tAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACttt                                                                                             <  2:344375/141‑1 (MQ=255)
                                               aaaTCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTa                                                                                            >  2:77098/1‑141 (MQ=255)
                                               aaaTCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTa                                                                                            >  2:15660/1‑141 (MQ=255)
                                                     tttCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTtc                                                                                      <  4:446626/141‑1 (MQ=255)
                                                     tttCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTtc                                                                                      <  1:12964/141‑1 (MQ=255)
                                                      ttCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTtct                                                                                     >  1:394398/1‑141 (MQ=255)
                                                       tCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTtctt                                                                                    >  2:988276/1‑140 (MQ=255)
                                                       tCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTtctc                                                                                    <  1:1032266/141‑1 (MQ=255)
                                                         gATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAatc                                                                                                                         <  2:829411/102‑1 (MQ=255)
                                                              ttCCAATAAATATATTAATGAAAATAAGATGTGCTCTTCATTGTCAATTTAACACATAATACGAAAAACAGTTATAAATATACTTCCAATTGAAATCACCGTATTACTCCTTTTTTTTCAACTTTACAATTGGCGTTTCGt                                                                             >  4:358906/1‑141 (MQ=255)
                                                               tCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGa                                                                                                                                         <  2:747123/81‑1 (MQ=255)
                                                               tCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGa                                                                                                                                         >  1:747123/1‑81 (MQ=255)
                                                               tCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTc                                                                            <  1:628611/141‑1 (MQ=255)
                                                                 cAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAg                                                                          <  4:312304/141‑1 (MQ=255)
                                                                   atatTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGAt                                                                        >  4:230486/1‑141 (MQ=255)
                                                                     atTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTc                                                                      <  1:29008/141‑1 (MQ=255)
                                                                       ttATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCaa                                                                    <  4:114762/141‑1 (MQ=255)
                                                                       ttATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCaa                                                                    <  2:1011380/141‑1 (MQ=255)
                                                                       ttATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCaa                                                                    >  2:261458/1‑141 (MQ=255)
                                                                       ttATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTAAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTTTAGTTTAGAAAGATTCaa                                                                    >  4:244354/1‑141 (MQ=255)
                                                                        tataTTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCaaa                                                                   >  3:125068/1‑141 (MQ=255)
                                                                                tGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCa                                                           <  1:326538/141‑1 (MQ=255)
                                                                                     aTAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCg                                                      >  2:549347/1‑141 (MQ=255)
                                                                                       aaGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGACATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGcc                                                    >  3:376904/1‑141 (MQ=255)
                                                                                        aGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCa                                                   >  1:153076/1‑141 (MQ=255)
                                                                                          aTGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCCGATTCAAACGTTTTCACTTCGCCAAg                                                 >  2:627859/1‑141 (MQ=255)
                                                                                          aTGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAg                                                 >  3:178783/1‑141 (MQ=255)
                                                                                          aTGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAg                                                 >  1:527439/1‑141 (MQ=255)
                                                                                          aTGTTATATACATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTCCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCTGATTCAAACGTTTTCACTTCGCCAAg                                                 >  2:233805/1‑141 (MQ=255)
                                                                                           tGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGc                                                >  2:282748/1‑141 (MQ=255)
                                                                                           tGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGc                                                >  4:209599/1‑141 (MQ=255)
                                                                                             ttATATTCATTGTTAATTTAACACATAGTAAGGAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTTCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTCGTCACTTCGCCCAGccg                                              <  2:384668/141‑2 (MQ=39)
                                                                                             ttATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCa                                              <  4:114882/141‑1 (MQ=255)
                                                                                             ttATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCa                                              <  4:114870/141‑1 (MQ=255)
                                                                                               atatTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCAtc                                            >  4:274079/1‑141 (MQ=255)
                                                                                                 atTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCAtctt                                          <  4:46507/141‑1 (MQ=255)
                                                                                                    cATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCAtctttct                                       <  1:295787/141‑1 (MQ=255)
                                                                                                      ttGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATctttcttt                                     >  3:125870/1‑141 (MQ=255)
                                                                                                       tGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTtg                                    <  2:999764/141‑1 (MQ=255)
                                                                                                              ttAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTTAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGc                             <  3:268213/141‑1 (MQ=255)
                                                                                                              ttAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGc                             >  3:126397/1‑141 (MQ=255)
                                                                                                               tAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCt                            <  3:118092/141‑1 (MQ=255)
                                                                                                                  cacaTAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCtttt                         >  3:54076/1‑141 (MQ=255)
                                                                                                                      tAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTAttt                     <  2:1050861/141‑1 (MQ=255)
                                                                                                                       ataataaaaagCAGTCATAAGATGATTTCAATTAGAAATCAATTTATGACTGTTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTAtttt                    >  1:655153/7‑141 (MQ=18)
                                                                                                                        gTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTg                   <  4:309868/141‑1 (MQ=255)
                                                                                                                         tAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGa                  >  1:139373/1‑141 (MQ=255)
                                                                                                                            gAAACACGGTCATATATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTGCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGt               <  2:289723/141‑1 (MQ=255)
                                                                                                                            gAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGt               <  1:987705/141‑1 (MQ=255)
                                                                                                                            aaaaaaCAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGt               <  2:738287/140‑1 (MQ=255)
                                                                                                                             aaaagCAGTCATAAGATGATTTCAATTAGAAATCAGTTTATGACTGTTTTTTATTATACTTTACATTGCTCGTTTCGTCAGCTTCAAACGTTTTCACTTCGCCAAGCCATCTGTCTTTGTGTTTGCTTTTATATTGACGtt              >  1:934597/1‑141 (MQ=21)
                                                                                                                             aaaagCAGTCATAAGATGATTTCAATTAGAAATCAATTTATGACTGTTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGtt              >  2:808841/1‑141 (MQ=18)
                                                                                                                             aaaaaCAGTCATAAATTGATTTCTAATTGTAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCGGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGtt              <  4:422144/141‑1 (MQ=255)
                                                                                                                             aaaaaCAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGtt              >  2:898603/1‑141 (MQ=255)
                                                                                                                              aaaaCAGTCATAAATTGATTTCTAATTGAAATCATCTCATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGttt             >  2:802570/1‑141 (MQ=255)
                                                                                                                                   cAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGa         >  2:230034/1‑141 (MQ=255)
                                                                                                                                   cAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGa         >  4:35544/1‑141 (MQ=255)
                                                                                                                                    aGTCATAATTTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGTCGTATTAGAc        <  2:13511/141‑1 (MQ=255)
                                                                                                                                    aGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGAc        <  1:321819/141‑1 (MQ=255)
                                                                                                                                    aGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGAc        <  3:230486/141‑1 (MQ=255)
                                                                                                                                    aGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGAc        <  1:740268/141‑1 (MQ=255)
                                                                                                                                      tCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTTACGTTTTaaaaaa      <  4:60136/141‑6 (MQ=255)
                                                                                                                                      tCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTGGACAt      <  3:203799/141‑1 (MQ=255)
                                                                                                                                          aaaTTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGACATaaaa  <  3:299942/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
ATTCAACTTAAAGATCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGACATAAAA  >  USA300TCH1516_ALE/2176125‑2176402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: