Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,203,709 T→C 5.3% K442K (AAA→AAG murF_2 ← UDP‑N‑acetylmuramoyl‑tripeptide‑‑D‑alanyl‑D‑ alanine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,203,7090TC 5.3% 341.8 / 7.1 112K442K (AAA→AAGmurF_2UDP‑N‑acetylmuramoyl‑tripeptide‑‑D‑alanyl‑D‑ alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (52/54);  new base C (4/2);  total (56/56)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 113 total aligned reads displayed.

AAAAGAAAAAGGCAATTTTGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGAATCATAAATATACTTC  >  USA300TCH1516_ALE/2203569‑2203847
                                                                                                                                            |                                                                                                                                          
aaaagaaaaagGCAATTTTGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAAt                                                                                                                                            >  4:366074/1‑141 (MQ=255)
      aaaagGCAATTTTGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATa                                                                                                                                      >  1:280793/1‑141 (MQ=255)
       aaagGCAATTTTGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATAc                                                                                                                                     >  2:90486/1‑141 (MQ=255)
        aagGCAATTTTGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATAcc                                                                                                                                    >  2:78474/1‑141 (MQ=255)
         agGCAATTTTGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCa                                                                                                                                   <  2:433724/141‑1 (MQ=255)
          ggCAATTTTGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCAc                                                                                                                                  >  4:169001/1‑141 (MQ=255)
           gCAATTTTGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACg                                                                                                                                 >  2:849096/1‑141 (MQ=255)
            cAATTTTGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGt                                                                                                                                <  1:382758/141‑1 (MQ=255)
              aTTTTGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCGCTCGACTAATCTCTATGAAATTAAAGCATTTACAACTTCTTCTAACTTCATACCACGTGa                                                                                                                              >  1:1085967/1‑141 (MQ=255)
               ttttGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGAt                                                                                                                             >  2:872679/1‑141 (MQ=255)
                  tGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCt                                                                                                                          <  2:1076412/141‑1 (MQ=255)
                  tGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCt                                                                                                                          >  2:383937/1‑141 (MQ=255)
                   gTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCtt                                                                                                                         >  2:949936/1‑141 (MQ=255)
                   gTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATCTCATACCACGTGATCCtt                                                                                                                         >  1:466400/1‑141 (MQ=255)
                     aTTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTa                                                                                                                       <  4:153817/141‑1 (MQ=255)
                     aTTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTa                                                                                                                       >  2:600520/1‑141 (MQ=255)
                      tttACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTGTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTaa                                                                                                                      >  1:614075/1‑141 (MQ=255)
                           acacaAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCGCTCGACTAATCTCTATGAAATTAAAGCATTTACAACTTCTTCTAACTTCATACCACGTGATCCTTTAACTAAt                                                                                                                 >  3:357092/1‑141 (MQ=255)
                            cacaAACCCACCTCTTGTTTGTAATCTGTTAGTATTAATTCAAATCATTTTTAGAAGTAAAAGGTCCCTCTACTAATCTCTATGAAATTAAAGGATTTACCTCTTCTACTAATTTCATACCACGTGGTCCTTTAACTAAtg                                                                                                                <  4:48261/141‑2 (MQ=255)
                            cacaAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATa                                                                                                                <  2:741699/141‑1 (MQ=255)
                              caAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATaca                                                                                                              >  1:819756/1‑141 (MQ=255)
                                   ccaccTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTc                                                                                                         <  1:754500/141‑1 (MQ=255)
                                   ccaccGCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTc                                                                                                         <  3:332497/141‑1 (MQ=255)
                                      ccTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATg                                                                                                      <  4:10780/141‑1 (MQ=255)
                                         cttcttTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATgcgc                                                                                                   <  1:987702/141‑1 (MQ=255)
                                            cttTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCttt                                                                                                <  2:507296/141‑1 (MQ=255)
                                              ttGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCAAGTGATCCTTTAACTAATACACGGTCATGCGCTTTTa                                                                                              <  3:409856/141‑1 (MQ=255)
                                                gTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTaaa                                                                                            >  3:101850/1‑141 (MQ=255)
                                                gTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTaaa                                                                                            >  2:122513/1‑141 (MQ=255)
                                                   aTCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGTTCATGCGCTTTTAAATct                                                                                         >  2:20552/1‑140 (MQ=255)
                                                    tCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGt                                                                                        <  1:351847/141‑1 (MQ=255)
                                                    tCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGt                                                                                        <  1:850782/141‑1 (MQ=255)
                                                     cTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCATTTAACTAATACACGGTCATGCGCTTTTAAATCGtt                                                                                       <  4:397386/141‑1 (MQ=255)
                                                     cTTTTAGTATTAATTAAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGtt                                                                                       >  1:189600/1‑141 (MQ=255)
                                                         tAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTttatt                                                                                   >  1:608060/1‑141 (MQ=255)
                                                          aGTATTAATTCAAATCATTTTTATAAGTAAAGGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTttatta                                                                                  <  1:586686/141‑1 (MQ=255)
                                                          aGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTttatta                                                                                  <  2:152303/141‑1 (MQ=255)
                                                           gTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTaa                                                                                 <  1:1003193/141‑1 (MQ=255)
                                                           gTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTaa                                                                                 <  1:483565/141‑1 (MQ=255)
                                                             aTTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTaaaa                                                                               >  1:61090/1‑141 (MQ=255)
                                                              ttAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAAc                                                                              <  2:709312/141‑1 (MQ=255)
                                                               tAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACt                                                                             >  1:966755/1‑141 (MQ=255)
                                                                aaTTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACtt                                                                            >  1:86192/1‑141 (MQ=255)
                                                                 aTTCAAATCATTTTTATAAGTAAAAGGTGNCTCAACNANTCTCTAGGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACTACGTGATCATGTAACTAATACACGTTCATGCGCGTTTGAATCGTTTATTAAAACTTc                                                                           >  3:194679/1‑141 (MQ=255)
                                                                   tCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTa                                                                         <  3:102946/141‑1 (MQ=255)
                                                                     aaaTCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATc                                                                       <  3:176803/141‑1 (MQ=255)
                                                                       aTCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCat                                                                     >  1:397247/1‑141 (MQ=255)
                                                                       aTCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTACCat                                                                     >  3:352170/1‑141 (MQ=255)
                                                                        tCATTTTTATAAGAACGCGGGCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCata                                                                    <  1:58409/141‑1 (MQ=255)
                                                                         cATTTTTATAAGTAAAAGGTCGCTCGACTAATCTCTATGAAATTAAAGCATTAACAACTTCTTCTAACTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGCTTGTTAAAACTTCTATCataa                                                                   >  2:280799/1‑140 (MQ=255)
                                                                          aTTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATc                                                                  >  2:528545/1‑141 (MQ=255)
                                                                             tttATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTc                                                               <  1:839814/141‑1 (MQ=255)
                                                                               tataAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCtt                                                             >  2:89653/1‑141 (MQ=255)
                                                                               tataAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCtt                                                             >  1:889561/1‑141 (MQ=255)
                                                                                ataAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCttt                                                            >  2:532503/1‑141 (MQ=255)
                                                                                ataAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCttt                                                            <  2:939813/141‑1 (MQ=255)
                                                                                ataAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCttt                                                            <  2:939817/141‑1 (MQ=255)
                                                                                  aaGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAg                                                          >  4:152342/1‑141 (MQ=255)
                                                                                   aGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGa                                                         >  2:901484/1‑141 (MQ=255)
                                                                                   aGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGa                                                         >  2:307558/1‑141 (MQ=255)
                                                                                     tAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAAt                                                       <  2:824154/141‑1 (MQ=255)
                                                                                      aaaaGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAAtt                                                      <  2:501913/141‑1 (MQ=255)
                                                                                         aGGACCCTCGACTAATCTCTATGAAATTAAAGCAATTACCACTTCTTCTAATGTCAGACCACTTGAGCCTTGAACTAATACACGGTCATGCGCTTTTAAATCGCTGAATAAAACTTCTAACAAATGGTCTTTAGAATTGag                                                   <  4:153376/141‑2 (MQ=255)
                                                                                              ccTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGtgtt                                              <  2:26722/141‑1 (MQ=255)
                                                                                               cTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGtgttg                                             <  1:833696/141‑1 (MQ=255)
                                                                                               cTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTATATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGtgttg                                             <  4:41277/141‑1 (MQ=255)
                                                                                               cTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCATTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGtgttg                                             <  1:1029772/141‑1 (MQ=255)
                                                                                                tCGACTAATCTCTATGAAATTAAAGCATTTACCACCTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGtgttgt                                            >  2:688117/1‑141 (MQ=255)
                                                                                                 cGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGtgttgtc                                           <  1:1029615/141‑2 (MQ=255)
                                                                                                 cGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTtgtg                                           <  1:648920/141‑1 (MQ=255)
                                                                                                     tAATCTCTATGAAATTAAAGTATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCttt                                       <  2:87921/141‑1 (MQ=255)
                                                                                                     tAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCttt                                       <  4:422164/141‑1 (MQ=255)
                                                                                                       aTCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCttttt                                     <  3:366074/141‑1 (MQ=255)
                                                                                                        tctcTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAGCTTCTATCATATCGTCTTTAGAATTGAAGTTTTTTGCTTTTTc                                    >  4:424285/1‑141 (MQ=255)
                                                                                                         ctctATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCg                                   >  1:461509/1‑141 (MQ=255)
                                                                                                           ctATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGAc                                 >  1:602677/1‑141 (MQ=255)
                                                                                                               gAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGt                             <  2:962075/141‑1 (MQ=255)
                                                                                                               gAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGt                             <  2:635117/141‑1 (MQ=255)
                                                                                                                 aaTTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTg                           >  4:238986/1‑141 (MQ=255)
                                                                                                                   ttAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCt                         <  2:326038/141‑1 (MQ=255)
                                                                                                                     aaaGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTgg                       <  3:161264/141‑1 (MQ=255)
                                                                                                                      aaGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGc                      <  2:210238/141‑1 (MQ=255)
                                                                                                                       aGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCttt                                                            >  2:332532/1‑102 (MQ=255)
                                                                                                                       aGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGcc                     >  2:245797/1‑141 (MQ=255)
                                                                                                                        gCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGccc                    <  1:568476/141‑1 (MQ=255)
                                                                                                                        gCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGccc                    <  4:278620/141‑1 (MQ=255)
                                                                                                                        gCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGAAAGAACGACATGTTGCTGGccc                    >  1:555710/1‑141 (MQ=255)
                                                                                                                          aTTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGa                  >  1:231892/1‑141 (MQ=255)
                                                                                                                           tttACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGaa                 >  3:344157/1‑141 (MQ=255)
                                                                                                                           tttACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGaa                 >  3:343386/1‑141 (MQ=255)
                                                                                                                           tttACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGaa                 <  1:814967/141‑1 (MQ=255)
                                                                                                                           tttACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGaa                 <  2:109662/141‑1 (MQ=255)
                                                                                                                             tACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGAATc               <  1:949936/141‑1 (MQ=255)
                                                                                                                               ccACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGACTCAt             >  2:80899/1‑141 (MQ=255)
                                                                                                                               ccACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGAATCAt             >  1:677333/1‑141 (MQ=255)
                                                                                                                               caaCTTCTTCTAACTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGCTTGTTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGAATCAt             >  4:27733/3‑141 (MQ=255)
                                                                                                                                  cttcttCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGAATCATaaa          <  1:1011715/141‑1 (MQ=255)
                                                                                                                                   ttcttcTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGAATCATAAat         >  2:912944/1‑141 (MQ=255)
                                                                                                                                   ttcttcTAACTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGCTTGTTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGAATCATAAat         <  4:357092/141‑1 (MQ=255)
                                                                                                                                        ctAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGAATCATAAATATACt    >  3:167188/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                          
AAAAGAAAAAGGCAATTTTGTATTTACACACAAAACCACCTCTTCTTTGTAATCTTTTAGTATTAATTCAAATCATTTTTATAAGTAAAAGGTCCCTCGACTAATCTCTATGAAATTAAAGCATTTACCACTTCTTCTAATTTCATACCACGTGATCCTTTAACTAATACACGGTCATGCGCTTTTAAATCGTTTATTAAAACTTCTATCATATCGTCTTTAGAATTGAAGTGTTGTGCTTTTTCGACATGTTGCTGGCCCGAATCATAAATATACTTC  >  USA300TCH1516_ALE/2203569‑2203847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: