Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,413,192 C→A 5.4% S516S (TCG→TCT) ‡ nphR ← Transcriptional activator NphR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,413,1920CA 5.4% 402.6 / 7.8 121S516S (TCG→TCT) ‡nphRTranscriptional activator NphR
Reads supporting (aligned to +/- strand):  ref base C (54/59);  new base A (4/3);  total (59/62)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 124 total aligned reads displayed.

AAATGACACATTGTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACA  >  USA300TCH1516_ALE/2413053‑2413332
                                                                                                                                           |                                                                                                                                            
aaaTGACACATTGTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCg                                                                                                                                             >  1:506773/1‑141 (MQ=255)
  aTGACACATTGTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGAt                                                                                                                                           <  1:389680/141‑1 (MQ=255)
   tGACACATTGTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGAtt                                                                                                                                          >  3:268634/1‑141 (MQ=255)
     acacATTGTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCa                                                                                                                                        <  1:15428/141‑1 (MQ=255)
     acacATTGTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCa                                                                                                                                        >  2:808231/1‑141 (MQ=255)
     acacATTGTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCa                                                                                                                                        <  1:653469/141‑1 (MQ=255)
       acaTTGTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACt                                                                                                                                      <  3:86690/141‑1 (MQ=255)
       acaTTGTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCATCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACt                                                                                                                                      >  3:125764/1‑141 (MQ=255)
       aaaTTATACTGTATGATTAAACTTCGATACAATCATCGTATCATTTTTATAAATAACAATACCTCGTAATTTATTAAGTATAAATAAAAAAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACt                                                                                                                                      <  4:414892/139‑1 (MQ=255)
        caTTGTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACtt                                                                                                                                     <  2:499880/141‑1 (MQ=255)
          ttGTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTtat                                                                                                                                   <  3:185643/141‑1 (MQ=255)
           tGTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTCAAATTTATTTCTTTGCTAGCTTCACTtata                                                                                                                                  >  1:113631/1‑141 (MQ=255)
            gTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATaa                                                                                                                                 <  3:105518/141‑1 (MQ=255)
             tACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAAt                                                                                                                                >  3:201932/1‑141 (MQ=255)
              aCTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAAtt                                                                                                                               <  2:14392/141‑1 (MQ=255)
                ttCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGa                                                                                                                             >  1:890978/1‑141 (MQ=255)
                 tCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGAc                                                                                                                            >  1:442281/1‑141 (MQ=255)
                  cATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACa                                                                                                                           <  3:423759/141‑1 (MQ=255)
                   aTGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACAt                                                                                                                          <  2:872854/141‑1 (MQ=255)
                   aTGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACAt                                                                                                                          >  4:407747/1‑141 (MQ=255)
                    tGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACAtg                                                                                                                         >  1:623524/1‑141 (MQ=255)
                                   aCAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCt                                                                                                          <  1:1084086/141‑1 (MQ=255)
                                   aCAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCGCGTTTCACTTATAATTGACATGTGCATAATAAGTCCt                                                                                                          <  3:12131/141‑1 (MQ=255)
                                    cAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTc                                                                                                         <  1:1017486/141‑1 (MQ=255)
                                      atcatcGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGa                                                                                                       >  1:972105/1‑141 (MQ=255)
                                      atcatcGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGa                                                                                                       >  2:700096/1‑141 (MQ=255)
                                      atcatcGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTCAAATTTATTTCTTTGCTAGCTTCACTTATAATTGACATGTGCATAATAAGTCCTCGa                                                                                                       >  2:60961/1‑141 (MQ=255)
                                         atcGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAAt                                                                                                    >  4:338225/1‑141 (MQ=255)
                                              gtCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGt                                                                                               >  3:396320/1‑141 (MQ=255)
                                                 aTTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGtata                                                                                            <  2:665953/141‑1 (MQ=255)
                                                   tttGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAAt                                                                                          <  3:414961/141‑1 (MQ=255)
                                                   tttGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAAt                                                                                          <  3:217553/141‑1 (MQ=255)
                                                          aataaCAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCaa                                                                                   >  2:341819/1‑141 (MQ=255)
                                                           ataaCAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCaaa                                                                                  >  3:381687/1‑141 (MQ=255)
                                                           ataaCAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCaaa                                                                                  >  3:193206/1‑141 (MQ=255)
                                                             aaCAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAg                                                                                >  1:895478/1‑141 (MQ=255)
                                                             aaCAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAg                                                                                <  1:789352/141‑1 (MQ=255)
                                                             aaCAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAg                                                                                >  2:458498/1‑141 (MQ=255)
                                                              aCAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGt                                                                               <  3:19178/141‑1 (MQ=255)
                                                              aCAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGt                                                                               <  2:995073/141‑1 (MQ=255)
                                                                aaTCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTc                                                                             <  1:97949/141‑1 (MQ=255)
                                                                 aTCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCa                                                                            >  3:306050/1‑141 (MQ=255)
                                                                  tCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAg                                                                           >  2:5007/1‑141 (MQ=255)
                                                                       cGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAAcc                                                                      <  4:231850/141‑1 (MQ=255)
                                                                        gTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCt                                                                     <  1:517017/141‑1 (MQ=255)
                                                                         tAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCtt                                                                    <  1:560215/141‑1 (MQ=255)
                                                                          aaTTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCtta                                                                   <  2:933859/141‑1 (MQ=255)
                                                                          aaTTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCtta                                                                   <  1:223276/141‑1 (MQ=255)
                                                                          aaTTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCtta                                                                   <  2:498032/141‑1 (MQ=255)
                                                                           aTTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCttat                                                                  >  3:237381/1‑141 (MQ=255)
                                                                            ttGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCttatt                                                                 <  2:666097/141‑1 (MQ=255)
                                                                              gATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTaa                                                               >  2:151012/1‑141 (MQ=255)
                                                                              gATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTaa                                                               <  2:892932/141‑1 (MQ=255)
                                                                               aTTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAat                                                              >  1:229116/1‑141 (MQ=255)
                                                                               aTTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAat                                                              >  1:55209/1‑141 (MQ=255)
                                                                                   aGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTTGATTCACTTATAATTGACATGTGCATAATAAGTCTTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCa                                                          >  2:777759/1‑141 (MQ=255)
                                                                                   aGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCa                                                          >  2:777764/1‑141 (MQ=255)
                                                                                    gTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAg                                                         <  2:506773/141‑1 (MQ=255)
                                                                                      atatTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGAc                                                       >  3:449048/1‑141 (MQ=255)
                                                                                       tatTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACa                                                      <  2:109276/141‑1 (MQ=255)
                                                                                        atTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACAt                                                     <  4:172882/141‑1 (MQ=255)
                                                                                        atTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACAt                                                     >  1:280138/1‑141 (MQ=255)
                                                                                         tttATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACAtt                                                    <  2:810670/141‑1 (MQ=255)
                                                                                             tGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCCTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATCACTATCAGACAATGTGa                                                >  2:102123/1‑141 (MQ=255)
                                                                                               aTAAAAATGAATATTGATAATCTAGTCAAATTTATTTCTTTGCTAGCTTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGTAACCTTATTAAAATCAGACATTGTGAtt                                              <  2:113631/141‑1 (MQ=255)
                                                                                                tAAAAATGAATATTGATAATCTAGTCAAATTTATTTCTTTGCTAGCTTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGTAACCTTATTAAAATCAGACATTGTGAttt                                             <  1:60961/141‑1 (MQ=255)
                                                                                                 aaaaaTGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGAtttt                                            <  1:1018868/141‑1 (MQ=255)
                                                                                                   aaaTGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGAtttttt                                          >  2:880609/1‑141 (MQ=255)
                                                                                                   aaaTGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGAtttttt                                          >  2:549687/1‑141 (MQ=255)
                                                                                                       gAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTCCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCttt                                      >  1:749537/1‑141 (MQ=255)
                                                                                                           atTGATAATCTAGTCAAATTTATTTCTTTGCTAGCTTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTAAGTAAACATATTAAAATCATACATTGTGATTTTTTCTTTTAAg                                  <  2:322459/141‑1 (MQ=255)
                                                                                                             tGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTc                                <  1:121048/141‑1 (MQ=255)
                                                                                                             tGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTc                                <  1:991474/141‑1 (MQ=255)
                                                                                                               aTAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCat                              <  2:453889/141‑1 (MQ=255)
                                                                                                                    cTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATtc                         <  3:272550/141‑1 (MQ=255)
                                                                                                                    cTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGCCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATtc                         <  4:413948/141‑1 (MQ=255)
                                                                                                                     tAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATtct                        >  4:397361/1‑141 (MQ=255)
                                                                                                                      agtcaaaTTTATTTCTTTGCTAGCTTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGTAACCTTATTAAAATCAGACATTGTGATTTTTTCTTTTAAGTCATTATtctc                       >  2:97533/5‑141 (MQ=255)
                                                                                                                        ttAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCAt                     <  1:483765/141‑1 (MQ=255)
                                                                                                                          aaaTTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTa                   <  3:114646/141‑1 (MQ=255)
                                                                                                                           aaTTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTaa                  <  2:910304/141‑1 (MQ=255)
                                                                                                                           aaTTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTaa                  <  1:372798/141‑1 (MQ=255)
                                                                                                                           aaTTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTaa                  >  1:522652/1‑141 (MQ=255)
                                                                                                                            atttatttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAAt                 <  2:96332/141‑1 (MQ=255)
                                                                                                                             tttatttCTTTGCTAGCTTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGTAACCTTATTAAAATCAGACATTGTGATTTTTTTTTTTTTTTCTTTATTCTCATTAATc                >  4:178104/1‑141 (MQ=255)
                                                                                                                               tatttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCaa              <  2:851259/141‑1 (MQ=255)
                                                                                                                                 tttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTTTTTTTTTTCATTATTCTCATTAATCAAAt            >  2:825881/1‑141 (MQ=255)
                                                                                                                                 tttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAATTCATTATTTACATTAATCAAAt            >  2:726532/1‑141 (MQ=255)
                                                                                                                                 tttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAAt            <  3:260666/141‑1 (MQ=255)
                                                                                                                                 tttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAAt            >  3:133512/1‑141 (MQ=255)
                                                                                                                                 tttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAAt            >  1:624564/1‑141 (MQ=255)
                                                                                                                                 tttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAAt            >  3:320802/1‑141 (MQ=255)
                                                                                                                                   tCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCt          <  1:379933/141‑1 (MQ=255)
                                                                                                                                      ttGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTTTTTTTTTTCATTATTCTCATTAATCAAATCTACa       >  2:414966/1‑141 (MQ=255)
                                                                                                                                       tGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACAt      >  2:398932/1‑141 (MQ=255)
                                                                                                                                        gCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATc     >  1:493855/1‑141 (MQ=255)
                                                                                                                                         cTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATca    >  1:530800/1‑141 (MQ=255)
                                                                                                                                           cGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATcaca  >  1:691303/1‑141 (MQ=255)
                                                                                                                                           cGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATcaca  <  2:339211/141‑1 (MQ=255)
                                                                                                                                           cGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATcaca  <  2:221974/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AAATGACACATTGTACTTCATGTTTGAACTTCGTTACAATCATCGTGTCATTTTGATAAATAACAATCCCTCGTAATTGATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACA  >  USA300TCH1516_ALE/2413053‑2413332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: