Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,413,272 T→A 5.1% I490F (ATT→TTT)  nphR ← Transcriptional activator NphR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,413,2720TA 5.1% 509.8 / 14.3 156I490F (ATT→TTT) nphRTranscriptional activator NphR
Reads supporting (aligned to +/- strand):  ref base T (73/75);  new base A (4/4);  total (77/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01

Only 100 of 160 total aligned reads displayed.

ATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTATTAGATGATCAATTT  >  USA300TCH1516_ALE/2413132‑2413412
                                                                                                                                            |                                                                                                                                            
aTTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAat                                                                                                                                              >  1:55209/1‑141 (MQ=255)
aTTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAat                                                                                                                                              >  1:229116/1‑141 (MQ=255)
    aGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTTGATTCACTTATAATTGACATGTGCATAATAAGTCTTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCa                                                                                                                                          >  2:777759/1‑141 (MQ=255)
    aGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCa                                                                                                                                          >  2:777764/1‑141 (MQ=255)
       atatTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGAc                                                                                                                                       >  3:449048/1‑141 (MQ=255)
        tatTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACa                                                                                                                                      <  2:109276/141‑1 (MQ=255)
         atTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACAt                                                                                                                                     <  4:172882/141‑1 (MQ=255)
              tGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGa                                                                                                                                >  2:507193/1‑141 (MQ=255)
              tGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGa                                                                                                                                <  1:469920/141‑1 (MQ=255)
              tGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCCTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATCACTATCAGACAATGTGa                                                                                                                                >  2:102123/1‑141 (MQ=255)
                aTAAAAATGAATATTGATAATCTAGTCAAATTTATTTCTTTGCTAGCTTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGTAACCTTATTAAAATCAGACATTGTGAtt                                                                                                                              <  2:113631/141‑1 (MQ=255)
                  aaaaaTGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGAtttt                                                                                                                            <  1:1018868/141‑1 (MQ=255)
                    aaaTGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGAtttttt                                                                                                                          >  2:880609/1‑141 (MQ=255)
                        gAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTCCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCttt                                                                                                                      >  1:749537/1‑141 (MQ=255)
                         aaTATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATAAGACATTGTGATTTTTTCtttt                                                                                                                     >  4:162972/1‑141 (MQ=255)
                              tGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTc                                                                                                                <  1:991474/141‑1 (MQ=255)
                              tGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTc                                                                                                                <  1:121048/141‑1 (MQ=255)
                               gATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCa                                                                                                               <  3:286105/141‑1 (MQ=255)
                                aTAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCat                                                                                                              <  2:453889/141‑1 (MQ=255)
                                     cTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGCCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATtc                                                                                                         <  4:413948/141‑1 (MQ=255)
                                         ttAAATTTATTTCTTTGCTCTATTCACTTATAATTGACATGTGCATAATAATTCCTCCAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTCATTTTTTCTTTTAAGTCATTATTCTCAt                                                                                                     <  4:45370/141‑1 (MQ=255)
                                         ttAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCAt                                                                                                     <  1:483765/141‑1 (MQ=255)
                                           aaaTTTATTTCTTTGCTCGATTCACTTATAATTNACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCTTTATTCNCATTa                                                                                                   >  1:133098/1‑141 (MQ=255)
                                            aaTTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTaa                                                                                                  <  1:372798/141‑1 (MQ=255)
                                            aaTTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTaa                                                                                                  >  1:522652/1‑141 (MQ=255)
                                             atttatttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAAt                                                                                                 <  2:96332/141‑1 (MQ=255)
                                                  tttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTTTTTTTTTTCATTATTCTCATTAATCAAAt                                                                                            >  2:825881/1‑141 (MQ=255)
                                                  tttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAATTCATTATTTACATTAATCAAAt                                                                                            >  2:726532/1‑141 (MQ=255)
                                                  tttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAAt                                                                                            >  3:133512/1‑141 (MQ=255)
                                                  tttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAAt                                                                                            >  1:624564/1‑141 (MQ=255)
                                                  tttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAAt                                                                                            <  3:260666/141‑1 (MQ=255)
                                                  tttCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAAt                                                                                            <  3:399036/141‑1 (MQ=255)
                                                    tCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCt                                                                                          <  1:379933/141‑1 (MQ=255)
                                                       ttGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTTTTTTTTTTCATTATTCTCATTAATCAAATCTACa                                                                                       >  2:414966/1‑141 (MQ=255)
                                                        tGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACAt                                                                                      >  2:398932/1‑141 (MQ=255)
                                                          cTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATca                                                                                    >  3:113396/1‑141 (MQ=255)
                                                          cTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATca                                                                                    >  1:530800/1‑141 (MQ=255)
                                                            cGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATcaca                                                                                  <  2:339211/141‑1 (MQ=255)
                                                                 cACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATaa                                                                             <  2:200941/141‑1 (MQ=255)
                                                                  aCTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAAt                                                                            <  2:473929/141‑1 (MQ=255)
                                                                   cTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAAtt                                                                           >  1:959460/1‑141 (MQ=255)
                                                                   cTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAAtt                                                                           <  4:335313/141‑1 (MQ=255)
                                                                    ttATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAAttt                                                                          >  4:6503/1‑141 (MQ=255)
                                                                    ttATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAAttt                                                                          <  3:407747/141‑1 (MQ=255)
                                                                    ttATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATCCTCATTAATCAAATCTACATCTCATATAAttt                                                                          >  4:408621/1‑141 (MQ=255)
                                                                       taATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGTAACCTTATTAAAATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAg                                                                       <  1:560116/141‑1 (MQ=255)
                                                                           tGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACg                                                                   <  1:154132/141‑1 (MQ=255)
                                                                            gACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTTTTTTTTTTTTTTATTCTCATTAATCAAATCTACATCACTTATAATTTTAGAACGt                                                                  >  2:1008967/1‑141 (MQ=255)
                                                                            gACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTCATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGt                                                                  <  3:386330/141‑1 (MQ=255)
                                                                             aCATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTg                                                                 <  2:341232/141‑1 (MQ=255)
                                                                              cATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGc                                                                >  3:56547/1‑141 (MQ=255)
                                                                                 gtgCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAAt                                                             >  4:207907/1‑141 (MQ=255)
                                                                                   gCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAg                                                           >  1:179152/1‑141 (MQ=255)
                                                                                      taataaGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGaca                                                        >  2:640276/1‑141 (MQ=255)
                                                                                       aataaGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTCATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGacac                                                       <  4:201932/141‑1 (MQ=255)
                                                                                         taaGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCt                                                     <  1:775097/141‑1 (MQ=255)
                                                                                           aGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGt                                                   <  1:1048598/141‑1 (MQ=255)
                                                                                            gTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGtt                                                  >  1:507215/1‑141 (MQ=255)
                                                                                             tCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTc                                                 >  1:297251/1‑141 (MQ=255)
                                                                                             tCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTc                                                 >  1:1029842/1‑141 (MQ=255)
                                                                                             tCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTc                                                 <  3:20927/141‑1 (MQ=255)
                                                                                               cTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTa                                               >  1:815111/1‑141 (MQ=255)
                                                                                                tCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTAt                                              >  1:505276/1‑141 (MQ=255)
                                                                                                tCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTAt                                              >  1:722486/1‑141 (MQ=255)
                                                                                                tCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTAt                                              <  2:895478/141‑1 (MQ=255)
                                                                                                  gAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTAttt                                            >  4:57801/1‑141 (MQ=255)
                                                                                                  gAAATTTTGTATAATCTGTCAAAAGTTCAGAAACCTTATTAATAACAGACATTGTCATTTCTTCCTTTAAGTCAATAATCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTAttt                                            <  1:647244/141‑1 (MQ=255)
                                                                                                   aaaTTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTg                                           >  1:268938/1‑141 (MQ=255)
                                                                                                    aaTTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTTTTTTTTTTTTTTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTCTTTGt                                          >  2:800708/1‑141 (MQ=255)
                                                                                                     aTTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTc                                         <  3:306180/141‑1 (MQ=255)
                                                                                                        ttGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTa                                      >  2:349118/1‑141 (MQ=255)
                                                                                                         tGTATAATTTGTCAAAAGTTCAGTAACCTTATTAAAATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACATGCAATAGACACCTGTTCTATTTGTCTTat                                     >  2:799713/1‑140 (MQ=255)
                                                                                                         tGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAg                                     <  1:812209/141‑1 (MQ=255)
                                                                                                          gTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGc                                    <  1:5007/141‑1 (MQ=255)
                                                                                                          gTATAATTTGTCAAAAGNTCAGAAACCTNATTANTATCAGACACTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGc                                    >  1:634876/1‑141 (MQ=255)
                                                                                                           tataATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCg                                   <  2:160975/141‑1 (MQ=255)
                                                                                                            ataATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCgg                                  <  3:349812/141‑1 (MQ=255)
                                                                                                             taATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGt                                 <  2:556510/141‑1 (MQ=255)
                                                                                                               aTTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTaa                               <  2:1063113/141‑1 (MQ=255)
                                                                                                                  tGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGAt                            <  1:608677/141‑1 (MQ=255)
                                                                                                                    tCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATaa                          >  3:404652/1‑141 (MQ=255)
                                                                                                                        aaGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTATGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATa                      >  2:605552/1‑141 (MQ=255)
                                                                                                                         aGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAg                     <  1:86730/141‑1 (MQ=255)
                                                                                                                         aGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAg                     <  1:900899/141‑1 (MQ=255)
                                                                                                                          gTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGt                    <  4:107368/141‑1 (MQ=255)
                                                                                                                           ttCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTc                   >  3:214285/1‑141 (MQ=255)
                                                                                                                              aGAAACCGTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTAt                >  1:859697/1‑141 (MQ=255)
                                                                                                                               gAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTAtt               >  4:18883/1‑141 (MQ=255)
                                                                                                                               gAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTAtt               >  2:305911/1‑141 (MQ=255)
                                                                                                                               gAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTAtt               >  2:706018/1‑141 (MQ=255)
                                                                                                                                   ccTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTATTAgat           >  1:558883/1‑141 (MQ=255)
                                                                                                                                     ttattaATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTATTAgatga         <  2:959460/141‑1 (MQ=255)
                                                                                                                                       attaATATCAGACATTGTGATTTTTTCTTTTAATTCATTATTCACATTGATCAAATCTACATCACATATAATTGTAGAACGTGCAATAGACACCTGTTCTATTGGTGTTAGCGGTAAGATAAAATAGTCTACTAGTTCATc       >  4:214809/1‑141 (MQ=255)
                                                                                                                                       attaATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTATTAGATGATc       >  3:53256/1‑141 (MQ=255)
                                                                                                                                       attaATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTATTAGATGATc       <  2:270825/141‑1 (MQ=255)
                                                                                                                                       attaATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTATTAGATGATc       <  2:30962/141‑1 (MQ=255)
                                                                                                                                        ttaATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTATTAGATGATCa      <  3:338225/141‑1 (MQ=255)
                                                                                                                                         taATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTATTAGATGATCaa     >  3:333679/1‑141 (MQ=255)
                                                                                                                                            tatCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTATTAGATGATCAAttt  >  3:120440/1‑141 (MQ=255)
                                                                                                                                            tatCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACAACTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTATTAGATGATCAAttt  >  1:593382/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ATTAAGTATATTTATGATAAAAATGAATATTGATAATCTAGTTAAATTTATTTCTTTGCTCGATTCACTTATAATTGACATGTGCATAATAAGTCCTCGAAATTTTGTATAATTTGTCAAAAGTTCAGAAACCTTATTAATATCAGACATTGTGATTTTTTCTTTTAAGTCATTATTCTCATTAATCAAATCTACATCACATATAATTTTAGAACGTGCAATAGACACCTGTTCTATTTGTCTTAGCGGTAAGATAAAATAGTCTATTAGATGATCAATTT  >  USA300TCH1516_ALE/2413132‑2413412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: