| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,524,206 | A→C | 5.0% | A142A (GCT→GCG) | nasD ← | Nitrite reductase [NAD(P)H] |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,524,206 | 0 | A | C | 5.0% | 463.2 / 13.4 | 155 | A142A (GCT→GCG) | nasD | Nitrite reductase [NAD(P)H] |
| Reads supporting (aligned to +/- strand): ref base A (68/76); new base C (3/5); total (74/81) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
Only 100 of 160 total aligned reads displayed.
GCTTTACGGTCTAGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATGATACTTCAATACCATT > USA300TCH1516_ALE/2524066‑2524346 | gCTTTACGGTCTAGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTa > 1:1004029/1‑141 (MQ=255)gCTTTACGGTCTAGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTa > 2:372988/1‑141 (MQ=255) tACGGTCTAGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTATTCTTatcta > 2:522036/1‑137 (MQ=255) tACGGTCTAGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCaa > 1:464560/1‑141 (MQ=255) aCGGTCTAGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAAt < 1:780641/141‑1 (MQ=255) aCGGTCTAGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAAt < 2:458432/141‑1 (MQ=255) cGGTCTAGTTGCATTTCCATCAACCATTCATTTATATTTTTTTCTGTTACTTCCATACCTTGATCTAATAAACTACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAAtt > 2:285151/1‑140 (MQ=255) cGGTCTAGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGCTCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTCCTTTTTAGTCTTAGCAAtt > 2:177731/1‑140 (MQ=255) cGGTCTAGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTATTAGCAATg > 3:430867/1‑141 (MQ=255) ggTCTAGCTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAAGCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTCGCAATGt < 1:175316/141‑1 (MQ=255) gTCTAGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGtt > 3:221376/1‑141 (MQ=255) tAGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTtcat < 2:149702/141‑1 (MQ=255) tAGTTGCACTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTtcat < 4:364501/141‑1 (MQ=255) aGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTtcatc < 1:217640/141‑1 (MQ=255) aGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTtcatc < 2:792233/141‑1 (MQ=255) ttGCATTTCCATCAACCATTCAGCTAGATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTcatcat < 2:901467/141‑1 (MQ=255) ttGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTcatcat < 3:407272/141‑1 (MQ=255) gCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACTCTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCAttt > 2:324758/1‑141 (MQ=255) gCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACTCTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCAttt > 2:790802/1‑141 (MQ=255) ttCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTc > 4:33750/1‑141 (MQ=255) tCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTat < 2:385392/141‑1 (MQ=255) cAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTATCAATGTTCATCATTTGTTCAGTatc > 4:24895/1‑141 (MQ=255) ccATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTatcatc < 4:283650/141‑1 (MQ=255) cATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTAtcatca < 3:142406/141‑1 (MQ=255) cATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTAtcatca > 1:933048/1‑141 (MQ=255) aGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGt < 1:736233/141‑1 (MQ=255) gCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCATTATCATCAAGCGtt > 4:307132/1‑141 (MQ=255) aaaTGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTtctc < 2:551890/141‑1 (MQ=255) aaTGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCGTCATTTGTTCAGTATCATCAAGCGTTCTcc < 4:280948/141‑1 (MQ=255) aaTGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTtctct < 1:1031774/141‑2 (MQ=255) aaTGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTcc > 1:122559/1‑141 (MQ=255) tGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTccaa < 1:896550/141‑1 (MQ=255) tAACACCGTTACTCCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTccaacc < 1:69242/141‑1 (MQ=255) acacCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTccaaccaa > 1:969797/1‑141 (MQ=255) cacCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAAt > 4:100541/1‑141 (MQ=255) cacCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAAt < 2:75631/141‑1 (MQ=255) acCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTTCAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAAtt < 1:537405/141‑1 (MQ=255) acCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAAtt > 3:132511/1‑141 (MQ=255) ccGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTa < 4:53466/141‑1 (MQ=255) ccGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTa > 1:966819/1‑141 (MQ=255) tACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACt < 3:318087/141‑1 (MQ=255) tACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATg < 1:595811/141‑1 (MQ=255) tACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATg < 3:116062/141‑1 (MQ=255) cTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCg < 4:406945/141‑1 (MQ=255) tGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGaa < 1:363035/141‑1 (MQ=255) tCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACct > 1:39708/1‑141 (MQ=255) cTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACctg > 1:306535/1‑141 (MQ=255) ataaACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCCTTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACctggaa > 1:514430/1‑141 (MQ=255) ataaACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACctggaa > 2:772692/1‑141 (MQ=255) ataaACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACctggaa > 2:993513/1‑141 (MQ=255) taaACCACGTGCACACTCTAAACCTAGTACTCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACGCTCGGTAATGTCGAACctggaac < 4:422051/141‑1 (MQ=255) taaACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACctggaac < 3:445611/141‑1 (MQ=255) taaACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACctggaac < 1:598135/141‑1 (MQ=255) aCCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAGTTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACctggaactgg < 4:121168/141‑1 (MQ=255) aCGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAAt > 1:244282/1‑141 (MQ=255) aCGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAAt > 1:192197/1‑141 (MQ=255) cGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATg > 1:660154/1‑141 (MQ=255) cGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATg > 3:293927/1‑141 (MQ=255) tGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGAc < 1:753284/141‑1 (MQ=255) cacTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAAt > 2:1075945/1‑141 (MQ=255) cctctAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATg < 1:341136/140‑1 (MQ=255) acTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATg < 2:1070050/141‑1 (MQ=255) ctAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCtt > 2:334725/1‑141 (MQ=255) tAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCttt < 1:613619/141‑1 (MQ=255) aaaCCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTa > 2:734348/1‑141 (MQ=255) aaaCCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTa < 3:31473/141‑1 (MQ=255) aaaCCTAGTAAGCCTCCACCAATTACAATTGCTTTCTTTTTATTCTTCGCAATGTTCATCATTTGTTCAGTATCATCAATCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTa < 1:10851/141‑1 (MQ=255) aCCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTTGTTTTTTCATTGTTCATCATTTGTTCAGTATCATCAAGGGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGCACGCGCATGACGAATGCTTTAGa > 2:380577/1‑141 (MQ=255) cTAGTAATTGTCCGCCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAGCCAATTACGCGCTGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAAc < 2:40150/141‑1 (MQ=255) cTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAAc < 2:915917/141‑1 (MQ=255) cTAGTAAGCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTCGCAATGTTCATCATTTGTTCAGTATCATCAATCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAAc > 2:714685/1‑141 (MQ=255) tAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGATTGCTTTaaaaaaaaa < 2:680110/141‑6 (MQ=255) tcctccACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGc < 4:180973/141‑1 (MQ=255) tcctccACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCCAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGc < 2:932731/141‑1 (MQ=255) cctccACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCa > 3:105032/1‑141 (MQ=255) tccACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCaaa > 4:219585/1‑141 (MQ=255) aaTTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCaa < 3:216691/141‑1 (MQ=255) aTTACAATTGCTTTCTTTTTAGTCTTCGCAATGTTCATCATTTGTTCAGTATCATCAATCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAAt > 2:536682/1‑141 (MQ=255) aTTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAAt > 1:766419/1‑141 (MQ=255) tACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAAttt > 3:127492/1‑141 (MQ=255) tACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAAttt > 3:166746/1‑141 (MQ=255) aaTTGCTTTCTTTTTAGTCTTCGCAATGTTCATCATTTGTTCAGTATCATCAATCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATc < 1:637284/141‑1 (MQ=255) aTTGCTTTCTTTTTTGTTTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCg > 4:167706/1‑141 (MQ=255) aTTGCTTTCTTTTTAGTTTTAGCAATGTTCATCATTTGTTCAGTATCATCAATCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCg > 2:876844/1‑141 (MQ=255) ttGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGt < 2:821376/141‑1 (MQ=255) gCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGAAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTAt < 2:941862/141‑1 (MQ=255) ctttctttTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATg > 1:420222/1‑141 (MQ=255) ctttctttTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATg > 1:1015153/1‑141 (MQ=255) tctttTTAGTCTTCGCAATGTTCATCATTTGTTCAGTATCATCAATCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATGATa > 2:607321/1‑141 (MQ=255) ctttTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATGATAc > 2:576484/1‑141 (MQ=255) ttttAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATGATACtt > 2:351785/1‑141 (MQ=255) attCTTTGTAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATGATACTTCAAt > 2:104047/3‑141 (MQ=255) aGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATGATACTTCAAt > 2:128396/1‑141 (MQ=255) gTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATGATACTTCAATa > 1:397855/1‑141 (MQ=255) gTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATGATACTTCAATa > 1:556818/1‑141 (MQ=255) gTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATGATACTTCAATa < 2:435663/141‑1 (MQ=255) tCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATGATACTTCAATAc < 1:486103/141‑1 (MQ=255) tCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACATGTCGCAAAAATCAATTTATCGTATGATACTTCAATAc < 2:965099/141‑1 (MQ=255) ttAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATGATACTTCAATACCa < 3:17604/141‑1 (MQ=255) aGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATGATACTTCAATACCAtt > 4:264013/1‑141 (MQ=255) | GCTTTACGGTCTAGTTGCATTTCCATCAACCATTCAGCTAAATGTAACACCGTTACTTCCATACCTTGATCTAATAAACCACGTGCACACTCTAAACCTAGTAATCCTCCACCAATTACAATTGCTTTCTTTTTAGTCTTAGCAATGTTCATCATTTGTTCAGTATCATCAAGCGTTCTCCAACCAATTACACTCGGTAATGTCGAACCTGGAACTGGAATGACGAATGCTTTAGAACCTGTCGCAAAAATCAATTTATCGTATGATACTTCAATACCATT > USA300TCH1516_ALE/2524066‑2524346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |