Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,559,065 C→T 5.3% A157A (GCC→GCT bcr_2 → Bicyclomycin resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,559,0650CT 5.3% 435.3 / 8.4 140A157A (GCC→GCTbcr_2Bicyclomycin resistance protein
Reads supporting (aligned to +/- strand):  ref base C (54/78);  new base T (4/4);  total (58/82)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 148 total aligned reads displayed.

ATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGAATTAAAGCAGGTGAATTTTAGTAG  >  USA300TCH1516_ALE/2558925‑2559205
                                                                                                                                            |                                                                                                                                            
aTTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGcc                                                                                                                                              <  2:703933/141‑1 (MQ=255)
aTTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGcc                                                                                                                                              <  1:144993/141‑1 (MQ=255)
aTTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGcc                                                                                                                                              <  2:703924/141‑1 (MQ=255)
aTTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGcc                                                                                                                                              >  1:235870/1‑141 (MQ=255)
   cAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGt                                                                                                                                           <  1:610199/141‑1 (MQ=255)
     aGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAACTTTAGTGGCAACGCCCTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTgg                                                                                                                                         <  1:917775/141‑1 (MQ=255)
     aGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTGGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTgg                                                                                                                                         <  1:315425/141‑1 (MQ=255)
       ttttAACTGGTGGTGGCGTCATCGTGATTGCAAAATCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTACTGGTAGTTATCGGCATCATCACTAGTCTTGCACCATTAGCCGGTGGAt                                                                                                                                       <  2:864490/140‑1 (MQ=255)
          tAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAg                                                                                                                                    >  2:1010740/1‑141 (MQ=255)
            aCTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCt                                                                                                                                  >  1:733044/1‑141 (MQ=255)
             cTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTCGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCtt                                                                                                                                 >  3:248531/1‑141 (MQ=255)
             cTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCtt                                                                                                                                 >  2:346372/1‑141 (MQ=255)
                     ggCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTa                                                                                                                         >  1:1041889/1‑141 (MQ=255)
                      gCGTCATCGTGATTGCCAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTAGCTAAATTTTTAGCATCTTTAATGGTAGTAAATGGCATCATCACTATTCTTGCACCATTAGCTGGTGGATTAGCTTTATCCGTAg                                                                                                                        >  3:218988/1‑141 (MQ=255)
                      gCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAg                                                                                                                        <  1:159692/141‑1 (MQ=255)
                         tCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCaa                                                                                                                     >  4:146645/1‑141 (MQ=255)
                         tCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCaa                                                                                                                     >  2:695277/1‑141 (MQ=255)
                            tCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTTATGGTAGTTAATGGCAGCATCACTATTCTTGCACCATTAGCTGGGGGATTAGCTTTATCCGTAGCAActt                                                                                                                  >  4:130326/1‑139 (MQ=255)
                                 aTTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGt                                                                                                             >  1:166965/1‑141 (MQ=255)
                                 aTTGCAAAAGCATCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATCTTTAGAATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTGACCGATGGATTAGCTTTATCCGTAGCAACATGTCga                                                                                                             >  4:293719/1‑140 (MQ=255)
                                  ttGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGtt                                                                                                            <  2:26241/141‑1 (MQ=255)
                                    gccaaaGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTAAATGGCATCATCACTATTCTTGCACCATTAGCTGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCt                                                                                                          >  3:377669/4‑141 (MQ=255)
                                    gCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCt                                                                                                          <  2:261586/141‑1 (MQ=255)
                                       aaaGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTAtt                                                                                                       >  1:632158/1‑141 (MQ=255)
                                         tgCCTCTTCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTCGCCGGTGAATTAGCTTTATCCGTAGCAACATGGCGTTCTAtttt                                                                                                     <  2:942511/140‑1 (MQ=255)
                                               tgctgGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAAt                                                                                               >  3:228854/1‑141 (MQ=255)
                                                gctgGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAAtt                                                                                              >  4:58467/1‑141 (MQ=255)
                                                gctgGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAAtt                                                                                              >  3:250827/1‑141 (MQ=255)
                                                  tgGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAAtttt                                                                                            <  2:368708/141‑1 (MQ=255)
                                                    gTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTaa                                                                                          >  3:158189/1‑141 (MQ=255)
                                                     tGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAAc                                                                                         <  1:217383/141‑1 (MQ=255)
                                                         aaaTTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTAtt                                                                                     >  3:110597/1‑141 (MQ=255)
                                                           aTTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATtgt                                                                                   >  2:356323/1‑141 (MQ=255)
                                                            tttAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATtgtg                                                                                  >  4:23430/1‑141 (MQ=255)
                                                             ttAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTgg                                                                                 <  2:591764/141‑1 (MQ=255)
                                                             ttAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTgg                                                                                 <  1:36873/141‑1 (MQ=255)
                                                              tAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGc                                                                                >  4:106490/1‑141 (MQ=255)
                                                               aGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCTCAATTTTAACTATTGTGGct                                                                               >  4:260280/1‑140 (MQ=255)
                                                                gTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCAc                                                                              <  1:175711/141‑1 (MQ=255)
                                                                gTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCAc                                                                              <  3:46000/141‑1 (MQ=255)
                                                                 tGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATCTTCACAATTTTAACTATTGTGGCACt                                                                             >  4:272419/1‑141 (MQ=255)
                                                                 tGGAAACGCACTCGCTAGATTTTTAGAATCATTAATGGTAGTTAATGGGCTAATAACTATTGTTGAACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCATTAAATTTTAACAAATTTAACTATTGTGGCACt                                                                             >  2:539864/1‑141 (MQ=255)
                                                                  ggCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACtc                                                                            >  2:1031410/1‑141 (MQ=255)
                                                                  ggCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTAAATGGCATCATCACTATTCTTGCACCATTAGCTGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACtc                                                                            >  2:930606/1‑141 (MQ=255)
                                                                   gCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACtca                                                                           <  2:968685/141‑1 (MQ=255)
                                                                   gCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACtca                                                                           <  1:149991/141‑1 (MQ=255)
                                                                     aaCGCACTCGTTAAATTTTTAGCATCTTTAATGGTAGTTAATGTCATCATCACTATTCTTGCACCATTAGCCGGTAGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACAATTGTGGCACtcagc                                                                         <  2:119762/141‑3 (MQ=255)
                                                                     aaCGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACtcatc                                                                         <  2:165035/141‑1 (MQ=255)
                                                                     aaCGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACtcatc                                                                         <  2:528216/141‑1 (MQ=255)
                                                                     aaCGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTCGCTGCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACtcatc                                                                         <  1:19258/141‑1 (MQ=255)
                                                                             cGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAAt                                                                 <  1:407669/141‑1 (MQ=255)
                                                                                 aaaTTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGc                                                             <  3:227995/141‑1 (MQ=255)
                                                                                 aaaTTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGc                                                             <  1:318316/141‑1 (MQ=255)
                                                                                 aaaTTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGc                                                             <  1:760129/141‑1 (MQ=255)
                                                                                   aTTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGt                                                           >  2:837183/1‑141 (MQ=255)
                                                                                   aTTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCAGCATTTTAATTGGCGt                                                           <  1:791582/141‑1 (MQ=255)
                                                                                    tttttAGCATCTTTAATGGTAGTAAATGGCATCATCACTATTCTTGCACCATTAGCTGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTc                                                          <  1:471998/141‑1 (MQ=255)
                                                                                       ttAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCt                                                       >  1:932944/1‑141 (MQ=255)
                                                                                       ttAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCt                                                       <  1:455406/141‑1 (MQ=255)
                                                                                        tAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCtt                                                      >  2:453117/1‑141 (MQ=255)
                                                                                        tAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCtt                                                      <  2:66942/141‑1 (MQ=255)
                                                                                         aGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTGCACAATTTTCACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTtc                                                     <  1:118148/141‑1 (MQ=255)
                                                                                          gCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTtct                                                    <  1:852132/141‑1 (MQ=255)
                                                                                            aTCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCa                                                  >  3:158638/1‑141 (MQ=255)
                                                                                              cTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAAt                                                <  4:53370/141‑1 (MQ=255)
                                                                                                   aTGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCt                                           <  4:54842/141‑1 (MQ=255)
                                                                                                   aTGGTAGTAAATGGCATCATCACTATTCTTGCACCATTAGCTGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCt                                           <  4:377669/141‑1 (MQ=255)
                                                                                                     ggTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTaa                                         <  1:860635/141‑1 (MQ=255)
                                                                                                      gtagtaAATGGCATCATCACTATTCTTGCACCATTAGCTGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTaaa                                        <  4:144278/141‑1 (MQ=255)
                                                                                                      gTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTaaa                                        <  3:436426/141‑1 (MQ=255)
                                                                                                      gTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTaaa                                        <  2:395881/141‑1 (MQ=255)
                                                                                                      gTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTaaa                                        >  2:501584/1‑141 (MQ=255)
                                                                                                        aGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTTTCAATTACCTAAAAc                                      >  3:136432/1‑141 (MQ=255)
                                                                                                        aGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAAc                                      <  3:146645/141‑1 (MQ=255)
                                                                                                        aGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAAc                                      <  3:330452/141‑1 (MQ=255)
                                                                                                         gTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACa                                     <  2:388719/141‑1 (MQ=255)
                                                                                                            aaTGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCt                                  >  1:1040047/1‑141 (MQ=255)
                                                                                                            aaTGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCt                                  <  2:399893/141‑1 (MQ=255)
                                                                                                            aaTGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCt                                  <  2:29534/141‑1 (MQ=255)
                                                                                                            aaTGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATACGTAGCCACTTGGCGTTCTTCTCCCACAACTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCt                                  <  3:317187/141‑1 (MQ=255)
                                                                                                            aaTGGCATCATCACTATGCGGGCAGCAGTATCCTTGGGATTAGCGTGAGCCGGAGCTACAGGGCGTTCTATTTTCACAATCTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCt                                  <  3:384861/141‑1 (MQ=255)
                                                                                                             aTGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTa                                 >  1:340654/1‑141 (MQ=255)
                                                                                                              tGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTaa                                >  2:210758/1‑141 (MQ=255)
                                                                                                                gCATCATCACTATTCTTGCACTATTAGCCGGTTGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAg                              <  2:383607/141‑1 (MQ=255)
                                                                                                                gCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAg                              <  1:42673/141‑1 (MQ=255)
                                                                                                                 catcatCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCCCAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGa                             <  1:452484/141‑1 (MQ=255)
                                                                                                                 catcatCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGa                             <  1:1038109/141‑1 (MQ=255)
                                                                                                                 catcatCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGa                             >  2:210829/1‑141 (MQ=255)
                                                                                                                     atcaCTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGaa                         >  2:1076762/1‑141 (MQ=255)
                                                                                                                     atcaCTACTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGaa                         >  1:860093/1‑141 (MQ=255)
                                                                                                                       caCTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGAAtt                       >  4:48862/1‑141 (MQ=255)
                                                                                                                         cTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTACTTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGAATTaa                     >  1:827186/1‑141 (MQ=255)
                                                                                                                           aTTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGAATTAAAg                   <  2:144078/141‑1 (MQ=255)
                                                                                                                             tCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGAATTAAAGCa                 <  1:501584/141‑1 (MQ=255)
                                                                                                                             tCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGAATTAAAGCa                 >  4:381989/1‑141 (MQ=255)
                                                                                                                                 gCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGAATTAAAGCAGGTg             <  1:117152/141‑1 (MQ=255)
                                                                                                                                    ccATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGAATTAAAGCAGGTGAAt          <  2:776928/141‑1 (MQ=255)
                                                                                                                                        tAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGAATTAAAGCAGGTGAATTTta      <  2:166965/141‑1 (MQ=255)
                                                                                                                                           ccGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTCTTGTGGAACTCATCATTTTTATTGGCGTGGCTTGTCCATACCTTAGAAAATCTAAAGATGAATTAAAGCAGGGGAATTTtagta   >  1:973306/1‑141 (MQ=255)
                                                                                                                                            cGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGAATTAAAGCAGGTGAATTTtagtag  >  2:801863/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGAATTAAAGCAGGTGAATTTTAGTAG  >  USA300TCH1516_ALE/2558925‑2559205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: