Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,559,841 C→A 8.5% intergenic (+35/+88) bcr_2 → / ← USA300HOU_RS13190 Bicyclomycin resistance protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,559,8410CA 8.5% 400.6 / 15.5 123intergenic (+35/+88)bcr_2/USA300HOU_RS13190Bicyclomycin resistance protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (50/62);  new base A (6/5);  total (56/67)
Fisher's exact test for biased strand distribution p-value = 5.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTGTAGT  >  USA300TCH1516_ALE/2559702‑2559979
                                                                                                                                            |                                                                                                                                          
tttAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatct                                                                                                                                           <  2:35980/141‑1 (MQ=255)
 ttAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatcta                                                                                                                                          <  1:939555/141‑1 (MQ=255)
 ttAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatcta                                                                                                                                          >  4:127013/1‑141 (MQ=255)
 ttAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatcta                                                                                                                                          >  1:31850/1‑141 (MQ=255)
  tAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatctat                                                                                                                                         <  2:244572/141‑1 (MQ=255)
   aaTACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatctatc                                                                                                                                        >  2:339998/1‑141 (MQ=255)
      aCATCACCATATATGATTATTATCTTCATTACAGCCTTTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAATAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTATCAAAGATATCTATCttt                                                                                                                                     >  1:1014644/1‑141 (MQ=255)
       cATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTg                                                                                                                                    <  1:627455/141‑1 (MQ=255)
          cACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCa                                                                                                                                 >  1:507135/1‑141 (MQ=255)
          cACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCa                                                                                                                                 >  2:933653/1‑141 (MQ=255)
             cATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAAAGAAGAACTCTGACAAAGATAGATATCTTTgctagg                                                                                                                               <  4:384784/141‑6 (MQ=37)
             cATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGATAGATATCTTTgctaggg                                                                                                                              <  1:43213/141‑7 (MQ=39)
             cATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAggg                                                                                                                              >  2:990098/1‑141 (MQ=255)
             cATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAggg                                                                                                                              <  3:87745/141‑1 (MQ=255)
             cATATATGATTATTATCGTCATTACAGCCATTCTATTAGTCAGTCTAAAAATGATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAggg                                                                                                                              <  2:821461/141‑1 (MQ=255)
               tatatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGtt                                                                                                                            <  2:221980/141‑1 (MQ=255)
                atatGATTATTATCTTCATTACAGCCATTCTATTAGTCATTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttc                                                                                                                           <  3:452545/141‑1 (MQ=255)
                atatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGATAGATATCTTTGCTAGGGttc                                                                                                                           <  2:150905/141‑1 (MQ=38)
                atatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttc                                                                                                                           <  1:882930/141‑1 (MQ=255)
                 tatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATCTCTAACTTTGTCAGGGttct                                                                                                                          >  1:58897/1‑141 (MQ=255)
                 tatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttct                                                                                                                          <  2:792960/141‑1 (MQ=255)
                 tatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttct                                                                                                                          <  2:351839/141‑1 (MQ=255)
                  atGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttctt                                                                                                                         >  2:710753/1‑141 (MQ=255)
                       tattatCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTa                                                                                                                    <  3:176700/141‑1 (MQ=255)
                             cTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAAtt                                                                                                              >  1:1049215/1‑141 (MQ=255)
                                   tACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATgaga                                                                                                        <  2:494361/141‑1 (MQ=255)
                                   tACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATgaga                                                                                                        <  3:236682/141‑1 (MQ=255)
                                   tACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATgaga                                                                                                        <  4:286180/141‑1 (MQ=255)
                                      aGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCg                                                                                                     >  3:13893/1‑141 (MQ=255)
                                      aGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCg                                                                                                     >  3:168462/1‑141 (MQ=255)
                                        ccATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGaa                                                                                                   >  1:706415/1‑141 (MQ=255)
                                           ttCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCGGGGTTCTTCTTTATGAATTATGAAATCCAATCt                                                                                                >  1:194481/1‑141 (MQ=255)
                                              tATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCa                                                                                             >  2:944259/1‑141 (MQ=255)
                                              tATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCa                                                                                             <  1:164267/141‑1 (MQ=255)
                                                ttAGTCCGTCTACAAAACATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAAc                                                                                           <  2:130118/141‑1 (MQ=255)
                                                ttAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAAc                                                                                           <  1:1057831/141‑1 (MQ=255)
                                                ttAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAAc                                                                                           <  3:223662/141‑1 (MQ=255)
                                                   gtcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaa                                                                                        >  1:535117/1‑141 (MQ=255)
                                                   gtcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaa                                                                                        >  2:532994/1‑141 (MQ=255)
                                                    tcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaaa                                                                                       >  1:714611/1‑141 (MQ=255)
                                                       gtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAAtt                                                                                    <  1:685775/141‑1 (MQ=255)
                                                        tcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTa                                                                                   >  2:223839/1‑141 (MQ=255)
                                                        tcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTa                                                                                   >  3:92644/1‑141 (MQ=255)
                                                        tcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTa                                                                                   <  1:390632/141‑1 (MQ=255)
                                                           aCAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGc                                                                                <  1:245040/141‑1 (MQ=255)
                                                           aCAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGc                                                                                >  3:160151/1‑141 (MQ=255)
                                                            cAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGcc                                                                               >  2:838504/1‑141 (MQ=255)
                                                              aaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCtt                                                                             <  1:419774/141‑1 (MQ=255)
                                                              aaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCtt                                                                             <  2:836358/141‑1 (MQ=255)
                                                               aTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTc                                                                            <  1:295063/141‑1 (MQ=255)
                                                                   tttACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAg                                                                        >  4:410138/1‑141 (MQ=255)
                                                                     tACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCa                                                                      >  3:399500/1‑141 (MQ=255)
                                                                        tttAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAgg                                                                   <  1:842232/141‑1 (MQ=255)
                                                                        tttAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAgg                                                                   <  3:305002/141‑1 (MQ=255)
                                                                        tatAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAAATAGAACCCTAGCAAAGATGTCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGAACGAATCTTCAACTAAAATTACGCCTACAAAACaaag                                                                   <  2:23727/139‑3 (MQ=255)
                                                                          tAAAAAGATTAAAAACCAACATGTCACATAACACTACAACATTATTAGACCCCTAGCAAAGATATCTATCTTTGTCAGGTTTCTTCTTTATGAATTATAAGATCGAATCTTCAACTAAAATTACGCCTTCCTAGCAAAGaa                                                                 <  4:271034/141‑2 (MQ=255)
                                                                           aaaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACa                                                                >  1:789052/1‑141 (MQ=255)
                                                                            aaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACAt                                                               <  2:716485/141‑1 (MQ=255)
                                                                            aaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACAt                                                               >  1:463095/1‑141 (MQ=255)
                                                                            aaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGACATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACAt                                                               <  1:1063207/141‑1 (MQ=255)
                                                                             aaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCATCCAAAATTACGCCTTCATAGCAAGGACAtt                                                              <  2:321431/141‑1 (MQ=255)
                                                                                 aTTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTa                                                          >  3:300156/1‑141 (MQ=255)
                                                                                     aaaaGCAACATGTCGCATAACACTTCAACATAACTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTGCTTCTTTATGAATTAAGAGAGCGAATCTTCAACTAAAATTACGCCTTCATGGTGAGGACATTTCTATTCa                                                      <  2:965093/141‑1 (MQ=255)
                                                                                     aaaaACAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGACTTCATAGCAAGGACATTTCTATTCa                                                      <  2:652164/141‑1 (MQ=255)
                                                                                          cAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAc                                                 >  4:23195/1‑141 (MQ=255)
                                                                                           aaCATGTCGCATAACACTTCAACATAATTAGTACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCTAGGACATTTCTATTCAATCAcg                                                <  2:1083175/141‑2 (MQ=255)
                                                                                           aaCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAcc                                                >  3:83329/1‑141 (MQ=255)
                                                                                            aCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAccc                                               >  2:976977/1‑141 (MQ=255)
                                                                                            aCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAccc                                               >  1:305915/1‑141 (MQ=255)
                                                                                                    cATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAg                                       <  2:1010271/141‑1 (MQ=255)
                                                                                                      tAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGc                                     <  4:312362/141‑1 (MQ=255)
                                                                                                       aaCACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCa                                    >  3:410522/1‑141 (MQ=255)
                                                                                                        acacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCGGGGTTCTTCTTTATGAATTATGAAATCCAATCTTCAACTAAAATTACGCCTTCATATCAAGTACATTTCTATTCAATCACCCTTTAACAGGCAt                                   >  1:924064/1‑141 (MQ=255)
                                                                                                         cacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATc                                  <  2:585800/141‑1 (MQ=255)
                                                                                                         cacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATc                                  <  1:628843/141‑1 (MQ=255)
                                                                                                           cTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCa                                >  4:117044/1‑141 (MQ=255)
                                                                                                             tCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCaaa                              <  1:55108/141‑1 (MQ=255)
                                                                                                                     aaTTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGt                       <  2:285802/141‑1 (MQ=255)
                                                                                                                      aTTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTa                      >  3:263315/1‑141 (MQ=255)
                                                                                                                      aTTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTACTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCAACCAAATTTCtata                      >  2:723263/1‑141 (MQ=255)
                                                                                                                       ttAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTaa                     >  2:710280/1‑141 (MQ=255)
                                                                                                                       ttAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTaa                     <  4:227239/141‑1 (MQ=255)
                                                                                                                       ttAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTaa                     <  4:227231/141‑1 (MQ=255)
                                                                                                                        tAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTaa                                          >  3:147307/1‑119 (MQ=255)
                                                                                                                        tAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTaa                                          <  4:147307/119‑1 (MQ=255)
                                                                                                                            aCCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatatat                >  1:631342/1‑141 (MQ=255)
                                                                                                                            aCCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatatat                <  2:466930/141‑1 (MQ=255)
                                                                                                                             cccTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATAtt               <  1:982570/141‑1 (MQ=255)
                                                                                                                              cctgacAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatat                  <  2:765276/132‑1 (MQ=38)
                                                                                                                              cctgacAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatat                  >  1:765276/6‑137 (MQ=38)
                                                                                                                               ctgacAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATAtttt             >  4:418122/5‑141 (MQ=255)
                                                                                                                                  gCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCa          >  2:768959/1‑141 (MQ=255)
                                                                                                                                   cAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCAc         >  2:618831/1‑141 (MQ=255)
                                                                                                                                   cAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCAc         >  1:56543/1‑141 (MQ=255)
                                                                                                                                    aaaGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACt        >  1:453501/1‑141 (MQ=255)
                                                                                                                                     aaGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACtt       <  2:1049215/141‑1 (MQ=255)
                                                                                                                                      aGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACttt      >  2:228279/1‑140 (MQ=255)
                                                                                                                                      aGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTg      <  3:301212/141‑1 (MQ=255)
                                                                                                                                      aGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGAGCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTg      <  3:86030/141‑1 (MQ=255)
                                                                                                                                       gATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTgt     <  2:162065/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTGTAGT  >  USA300TCH1516_ALE/2559702‑2559979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: