Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,642,644 T→A 5.1% intergenic (‑74/+65) USA300HOU_RS13595 ← / ← yccS hypothetical protein/Inner membrane protein YccS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,642,6440TA 5.1% 448.2 / 10.5 142intergenic (‑74/+65)USA300HOU_RS13595/yccShypothetical protein/Inner membrane protein YccS
Reads supporting (aligned to +/- strand):  ref base T (63/69);  new base A (4/3);  total (70/72)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

Only 100 of 150 total aligned reads displayed.

CTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCTGATTGAATATAATTATACTGT  >  USA300TCH1516_ALE/2642504‑2642784
                                                                                                                                             |                                                                                                                                            
cTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACAt                                                                                                                                              <  2:61400/141‑1 (MQ=255)
      ggcGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATTAAACATATTAAACACAttttttt                                                                                                                                        >  2:56232/3‑141 (MQ=255)
           ttAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACt                                                                                                                                   <  1:887835/141‑1 (MQ=255)
             aaTTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTcc                                                                                                                                 <  2:982536/141‑1 (MQ=255)
             aaTTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTcc                                                                                                                                 <  2:843305/141‑1 (MQ=255)
               ttACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCTTTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCg                                                                                                                               <  2:472269/141‑1 (MQ=255)
               ttACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCg                                                                                                                               <  2:767922/141‑1 (MQ=255)
                 aCTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTc                                                                                                                             >  1:775725/1‑141 (MQ=255)
                 aCTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTc                                                                                                                             >  2:723965/1‑141 (MQ=255)
                 aCTTCAAATTCTTTCCATTTTTCGGGGTAACCTTCCATTGTAAAGCGCATGTTCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTc                                                                                                                             <  1:431929/141‑1 (MQ=255)
                   ttCAAATTCTTTCCATTTTTCGGGGTAACCTTTTATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCat                                                                                                                           >  2:123564/1‑141 (MQ=255)
                    tCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCata                                                                                                                          <  2:333305/141‑1 (MQ=255)
                       aaTTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCataata                                                                                                                       <  1:668051/141‑1 (MQ=255)
                          tCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACt                                                                                                                    >  4:17936/1‑141 (MQ=255)
                           cTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACtt                                                                                                                   >  1:130753/1‑141 (MQ=255)
                               ccATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGa                                                                                                               <  2:1039244/141‑1 (MQ=255)
                               ccATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGa                                                                                                               <  1:899974/141‑1 (MQ=255)
                               ccATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGa                                                                                                               <  3:424362/141‑1 (MQ=255)
                               ccATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGa                                                                                                               <  4:443844/141‑1 (MQ=255)
                                 aTTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACa                                                                                                             <  1:578908/141‑1 (MQ=255)
                                 aTTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACa                                                                                                             <  1:174082/141‑1 (MQ=255)
                                  tttttCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGCCATAATAACTTCAGACaa                                                                                                            >  1:889050/1‑141 (MQ=255)
                                      tCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGt                                                                                                        <  1:90950/141‑1 (MQ=255)
                                                   tCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGa                                                                                           <  3:335903/141‑1 (MQ=255)
                                                   tCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGa                                                                                           <  3:335966/141‑1 (MQ=255)
                                                    cATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGAt                                                                                          <  2:674971/141‑1 (MQ=255)
                                                    cATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACAATTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGAt                                                                                          >  4:165387/1‑141 (MQ=255)
                                                      ttGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATaa                                                                                        >  1:523276/1‑141 (MQ=255)
                                                      aatgaaaGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATaa                                                                                        >  4:390326/5‑141 (MQ=255)
                                                          aaaGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAatat                                                                                    >  4:337780/1‑141 (MQ=255)
                                                          aaaGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACACATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAatat                                                                                    >  2:836852/1‑141 (MQ=255)
                                                            aGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATTCCATCATAATTATAAAATGAAACATACTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCCGACAAACGTTAGTCTGACCTGATAAATAttt                                                                                  <  2:359508/141‑1 (MQ=255)
                                                             gggCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTa                                                                                 <  3:408194/141‑1 (MQ=255)
                                                                cATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAAtt                                                                              >  4:107977/1‑141 (MQ=255)
                                                                cATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAAtt                                                                              <  2:172037/141‑1 (MQ=255)
                                                                cATTTACTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACAATTTTTTAGCCTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTTAtt                                                                              <  4:273535/141‑1 (MQ=255)
                                                                 aTTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAAttt                                                                             >  1:405250/1‑141 (MQ=255)
                                                                  tttCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAAtttt                                                                            >  3:346408/1‑141 (MQ=255)
                                                                  tttCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAAtttt                                                                            >  3:342380/1‑141 (MQ=255)
                                                                  tttCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCGGATAAATATTTAAtttt                                                                            >  3:3567/1‑141 (MQ=255)
                                                                   ttCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATACATATTTAATTTTa                                                                           >  2:626270/1‑141 (MQ=255)
                                                                    tCCTTAACCTCCAATATGTTATAATTCATGTTATATCCCACCATAATTATAAAATGAAACATATTCAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTNATTTTaa                                                                          >  2:812135/1‑141 (MQ=255)
                                                                    tCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTaa                                                                          >  1:665308/1‑141 (MQ=255)
                                                                       ttAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACg                                                                       <  1:54410/141‑1 (MQ=255)
                                                                       ttAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACg                                                                       <  2:386089/141‑1 (MQ=255)
                                                                         aaCCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCTCGTCATAATAACTTCAAACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGtt                                                                     >  2:1082384/1‑141 (MQ=255)
                                                                         aaCCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGtt                                                                     >  4:77201/1‑141 (MQ=255)
                                                                              ccAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACTTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCCTGt                                                                >  2:635508/1‑141 (MQ=255)
                                                                              ccAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGt                                                                >  2:359043/1‑141 (MQ=255)
                                                                               cAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGtc                                                               >  1:522087/1‑141 (MQ=255)
                                                                                 atatGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGtctc                                                             <  2:775725/141‑1 (MQ=255)
                                                                                   atGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTATTTTCTTTTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGtctctc                                                           >  2:966676/1‑141 (MQ=255)
                                                                                   atGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACAATTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTTATTTTAAACGTTCTTGtctctc                                                           <  4:379083/141‑1 (MQ=255)
                                                                                    tGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGtctctct                                                          <  1:16319/141‑1 (MQ=255)
                                                                                     gTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGtctctctc                                                         <  4:81233/141‑1 (MQ=255)
                                                                                     gTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGtctctctc                                                         >  1:723460/1‑141 (MQ=255)
                                                                                     gTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGtctctctc                                                         <  1:812018/141‑1 (MQ=255)
                                                                                      ttATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCa                                                        >  3:66982/1‑141 (MQ=255)
                                                                                       tataATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCAc                                                       <  2:165008/141‑1 (MQ=255)
                                                                                       tataATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCAc                                                       >  1:65970/1‑141 (MQ=255)
                                                                                       tataATTCATGTTATATACCACAATAATTATAAAATGAAACATATTAAACACATTGTTTTAAACTCCCGACATAATAACTTCAGGCGAACGATGGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCAc                                                       >  3:130342/1‑141 (MQ=255)
                                                                                        ataATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACg                                                      <  1:317542/141‑1 (MQ=255)
                                                                                        ataATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACg                                                      <  1:639488/141‑1 (MQ=255)
                                                                                        ataATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACg                                                      <  2:890963/141‑1 (MQ=255)
                                                                                         taATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTTTTACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTATCTCTCAcc                                                      >  2:366419/1‑140 (MQ=255)
                                                                                             tCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAAc                                                 >  1:73974/1‑141 (MQ=255)
                                                                                             tCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAAc                                                 >  1:74141/1‑141 (MQ=255)
                                                                                              cATGTTATATACCACCATAATTATAAAATGAAAAATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAAcc                                                >  1:562168/1‑141 (MQ=255)
                                                                                                tGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCAt                                              >  2:878911/1‑141 (MQ=255)
                                                                                                tGTTATATACCACCATAAGTATAAAAGGAAACATAATAAACTCACTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGTCCTGATAAATATATAATTTTAAACGTTCTTGTTTCTCTCACGaaatctat                                              >  4:104058/1‑136 (MQ=255)
                                                                                                 gTTATATACCACCATAATTATAAAATGAAACATATTAAACACCTTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCAtt                                             >  1:1080004/1‑141 (MQ=255)
                                                                                                 gTTATATACCACCATAATTATAAAATGAAACATATTAAACACAATTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTTATTTTAAACGTTCTTGTCTCTCTCACGAAAACCAtt                                             >  2:302578/1‑141 (MQ=255)
                                                                                                  ttATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATtg                                            >  1:122344/1‑141 (MQ=255)
                                                                                                     tatgccaccaTAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTgtgt                                         >  2:276303/5‑141 (MQ=255)
                                                                                                        accaccATAATTATAAAATGAAACATATTAAACACATTTTTTTTTTTTTTTTTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATAGTGTTGa                                      >  2:809813/1‑141 (MQ=255)
                                                                                                         ccaccaTAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGaa                                     >  1:350400/1‑141 (MQ=255)
                                                                                                           accaTAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAata                                   >  3:387190/1‑141 (MQ=255)
                                                                                                             caTAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTTAATAtt                                 >  2:693261/1‑141 (MQ=255)
                                                                                                              aTAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATtc                                <  3:118907/141‑1 (MQ=255)
                                                                                                              aTAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATtc                                <  3:107969/141‑1 (MQ=255)
                                                                                                                 aTTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATtcatc                             <  2:483727/141‑1 (MQ=255)
                                                                                                                 aTTATAAAATGAAACATATTAAACACAATTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTTATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATtcatc                             >  2:214893/1‑141 (MQ=255)
                                                                                                                    ataAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCAtt                          <  1:972754/141‑1 (MQ=255)
                                                                                                                       aaaTGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCt                       >  1:752941/1‑141 (MQ=255)
                                                                                                                         aTGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAAACATTGTGTTGAATATTCATCATTGCTGa                     >  2:871122/1‑141 (MQ=255)
                                                                                                                          tGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCTGAt                    <  3:437770/141‑1 (MQ=255)
                                                                                                                           gAAACATATTAAACACATTTTTTTAGACTCCCGTCATAGTAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCTGAtt                   >  1:575986/1‑141 (MQ=255)
                                                                                                                           gAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCTGAtt                   <  2:667960/141‑1 (MQ=255)
                                                                                                                           gAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCTGAtt                   <  2:67865/141‑1 (MQ=255)
                                                                                                                             aaCATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCTGATTGa                 <  3:401493/141‑1 (MQ=255)
                                                                                                                             aaCATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCTGATTGa                 <  1:62186/141‑1 (MQ=255)
                                                                                                                               cATATTAAACACATTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCTGATTGAata              >  4:426072/1‑141 (MQ=255)
                                                                                                                                  atTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCTGATTGAAtata            <  2:891709/141‑1 (MQ=255)
                                                                                                                                    tAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCTGATTGAATATAAt          <  3:55707/141‑1 (MQ=255)
                                                                                                                                    tAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCTGATTGAATATAAt          <  1:760879/141‑1 (MQ=255)
                                                                                                                                         acaTTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCTGATTGAATATAATTATAc     >  2:711982/1‑141 (MQ=255)
                                                                                                                                           aTTTTNTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAANATTCATCATTGCTGATTGAATATAATTATACtg   <  1:136750/141‑1 (MQ=255)
                                                                                                                                             tttttttAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTg                                       >  3:213263/1‑104 (MQ=255)
                                                                                                                                             tttttttAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTg                                       <  4:213263/104‑1 (MQ=255)
                                                                                                                                             tttttttAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTg                                       <  4:213368/104‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
CTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTCACGAAAACCATTGTGTTGAATATTCATCATTGCTGATTGAATATAATTATACTGT  >  USA300TCH1516_ALE/2642504‑2642784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: