BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	178,248	T→G	5.3%	Y75D (TAT→GAT)	capD →	UDP‑glucose 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	178,248	0	T	G	5.3%	410.0 / 10.0	130	Y75D (TAT→GAT)	capD	UDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand): ref base T (52/71); new base G (4/3); total (56/74)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 137 total aligned reads displayed.

TATTAAAGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTAAAAAAGTCATATGT  >  USA300TCH1516_ALE/178109‑178388

tatTAAAGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAAGAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTtta                                                                                                                                             <  1:565286/141‑1 (MQ=255)

 atTAAAGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTttat                                                                                                                                            <  2:653311/141‑1 (MQ=255)

    aaaGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTAc                                                                                                                                         >  1:804499/1‑141 (MQ=255)

    aaaGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTAc                                                                                                                                         >  1:695718/1‑141 (MQ=255)

    aaaGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTAc                                                                                                                                         >  1:696779/1‑141 (MQ=255)

    aaaGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAAATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTAc                                                                                                                                         >  2:196605/1‑141 (MQ=255)

     aaGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACg                                                                                                                                        >  3:142006/1‑141 (MQ=255)

     aaGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACg                                                                                                                                        <  3:315791/141‑1 (MQ=255)

     aaGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACg                                                                                                                                        <  1:1064438/141‑1 (MQ=255)

      aGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGt                                                                                                                                       <  2:686624/141‑1 (MQ=255)

      aGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGt                                                                                                                                       >  2:502332/1‑141 (MQ=255)

          aTTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTc                                                                                                                                   <  2:987141/141‑1 (MQ=255)

           ttCGTATTTTTTCACGCAATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAGATTAATGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAATAGCGATGCGAGATGTTTATTACGTATTcc                                                                                                                                  <  2:675696/141‑1 (MQ=255)

             cGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCAt                                                                                                                                <  3:359317/141‑1 (MQ=255)

              gTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATg                                                                                                                               >  3:243348/1‑141 (MQ=255)

                  tttttCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATgcagc                                                                                                                           <  2:26486/141‑1 (MQ=255)

                  tttttCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATgcagc                                                                                                                           <  3:32655/141‑1 (MQ=255)

                  tttttCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATgcagc                                                                                                                           <  2:960115/141‑1 (MQ=255)

                   ttttCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATgcagca                                                                                                                          >  2:579967/1‑141 (MQ=255)

                      tCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCt                                                                                                                       <  3:312028/141‑1 (MQ=255)

                      tCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCt                                                                                                                       <  2:851327/141‑1 (MQ=255)

                      tCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCt                                                                                                                       <  3:439785/141‑1 (MQ=255)

                        aCGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCttt                                                                                                                     >  2:485565/1‑141 (MQ=255)

                        aCGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCtgt                                                                                                                     >  3:226129/1‑139 (MQ=255)

                        aCGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGAttt                                                                                                                     >  4:327921/1‑141 (MQ=255)

                         cgcgATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTa                                                                                                                    >  2:751839/1‑141 (MQ=255)

                          gcgATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTaa                                                                                                                   >  1:553078/1‑141 (MQ=255)

                                     aaaCAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCg                                                                                                        >  4:426584/1‑141 (MQ=255)

                                         aaGATGACATTCGAAAAAAATATAATAAATCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCAt                                                                                                    >  2:594760/1‑141 (MQ=255)

                                          aGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTATAAAGCAAGTGCCGTCAtg                                                                                                   >  1:772178/1‑141 (MQ=255)

                                           gATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCAtgt                                                                                                  >  3:179883/1‑141 (MQ=255)

                                           gATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCAtgt                                                                                                  >  2:785248/1‑141 (MQ=255)

                                            aTGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCAtgtg                                                                                                 <  1:495084/141‑1 (MQ=255)

                                             agACAATCAAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGa                                                                                                <  2:703609/140‑1 (MQ=255)

                                                 aTTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTc                                                                                            <  2:51230/141‑1 (MQ=255)

                                                 aTTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTc                                                                                            <  1:489857/141‑1 (MQ=255)

                                                   acGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCtt                                                                                          <  2:459108/140‑1 (MQ=255)

                                                      aaaaaaaTATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTcc                                                                                       <  3:156820/141‑1 (MQ=255)

                                                      aaaaaaaTATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTcc                                                                                       <  2:1048347/141‑1 (MQ=255)

                                                        aaaaaaATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAg                                                                                     >  1:576583/1‑141 (MQ=255)

                                                         aaaaTATAATAGTTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGt                                                                                    <  4:136901/141‑1 (MQ=255)

                                                           aaTATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTg                                                                                  <  3:218110/141‑1 (MQ=255)

                                                            atatAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGa                                                                                 <  2:842043/141‑1 (MQ=255)

                                                            atatAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGa                                                                                 <  1:558561/141‑1 (MQ=255)

                                                            atatAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGa                                                                                 <  1:130830/141‑1 (MQ=255)

                                                              ataataATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAgg                                                                               <  2:800174/141‑1 (MQ=255)

                                                              ataataATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAgg                                                                               <  4:396100/141‑1 (MQ=255)

                                                              ataataATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTACAGTTGAgg                                                                               >  1:274813/1‑141 (MQ=255)

                                                                 ataatTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAg                                                                            <  4:94374/141‑1 (MQ=255)

                                                                      taaaaaTTAAAGTTCTACAGTGGTGATGTGCGTGAGAGTCAAAGTGTAGAAACAGCTATGCGAGATGTTTATTACGTATTCCATGCAGCAGATTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAg                                                                       <  4:164193/139‑1 (MQ=255)

                                                                        aaaaTTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAg                                                                                                             >  1:877597/1‑101 (MQ=255)

                                                                         aaaTTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACa                                                                    <  2:691749/141‑1 (MQ=255)

                                                                           aTTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACaaa                                                                  <  1:57833/141‑1 (MQ=255)

                                                                            ttAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAat                                                                 >  3:217496/1‑141 (MQ=255)

                                                                            ttAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAat                                                                 <  3:71418/141‑1 (MQ=255)

                                                                            ttAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAat                                                                 >  1:443217/1‑141 (MQ=255)

                                                                                aGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAAtatta                                                             <  2:525843/141‑1 (MQ=255)

                                                                                   tCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTg                                                          <  2:259491/141‑1 (MQ=255)

                                                                                    cTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTgg                                                         <  2:116943/141‑1 (MQ=255)

                                                                                     tACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGt                                                        <  1:132579/141‑1 (MQ=255)

                                                                                       cATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTAc                                                      <  3:30368/141‑1 (MQ=255)

                                                                                          tGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGa                                                   <  2:162299/141‑1 (MQ=255)

                                                                                          tGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGa                                                   <  3:146611/141‑1 (MQ=255)

                                                                                           ggTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGaa                                                  >  4:89637/1‑141 (MQ=255)

                                                                                            gTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCCGTTGGGGCAGTGAACACAAATATTATTGGTACAGaaa                                                 <  3:217271/141‑1 (MQ=255)

                                                                                               aTGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATg                                              <  2:933736/141‑1 (MQ=255)

                                                                                                  tgCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCt                                           <  2:511512/141‑1 (MQ=255)

                                                                                                    cGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTa                                         >  1:64452/1‑141 (MQ=255)

                                                                                                         tAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAg                                    <  2:851371/141‑1 (MQ=255)

                                                                                                            tCACAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGc                                 <  1:325607/141‑1 (MQ=255)

                                                                                                             cAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCt                                >  1:1025660/1‑141 (MQ=255)

                                                                                                               aaGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTAt                              >  1:251301/1‑141 (MQ=255)

                                                                                                               aaGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTAt                              <  3:22017/141‑1 (MQ=255)

                                                                                                               aaGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTAt                              <  4:243348/141‑1 (MQ=255)

                                                                                                               aaGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAACGTCTTACAAAGTGCTAt                              >  2:317933/1‑141 (MQ=255)

                                                                                                                aGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTAtt                             >  1:468947/1‑141 (MQ=255)

                                                                                                                aGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTAtt                             >  2:764167/1‑141 (MQ=255)

                                                                                                                aGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTAtt                             >  2:65444/1‑141 (MQ=255)

                                                                                                                 gtgtAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATtc                            <  1:1004136/141‑1 (MQ=255)

                                                                                                                 gtgtAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATtc                            <  2:292049/141‑1 (MQ=255)

                                                                                                                 gtgtAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATtc                            <  2:387943/141‑1 (MQ=255)

                                                                                                                     aGAAACAGCAATGCGAGATGTTTA‑TACGTAGTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAATATGTCTTACAAAGTGCTATTCATCaa                       <  4:213745/141‑1 (MQ=255)

                                                                                                                       aaaCAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCaaa                      >  1:630505/1‑141 (MQ=255)

                                                                                                                        aaCAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCaaaa                     >  2:600570/1‑141 (MQ=255)

                                                                                                                         aCAGCAATGCGAGATGTTTATTGCGTAGTCCGTGCAGGAGGTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAAt                    <  2:363457/141‑1 (MQ=255)

                                                                                                                         aCAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAAt                    <  4:146176/141‑1 (MQ=255)

                                                                                                                           aGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGt                  >  1:99644/1‑141 (MQ=255)

                                                                                                                           aGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGt                  >  4:142383/1‑141 (MQ=255)

                                                                                                                           aGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGt                  <  1:632226/141‑1 (MQ=255)

                                                                                                                            gCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGtt                 <  1:407794/141‑1 (MQ=255)

                                                                                                                                tGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTaaaa             >  4:135419/1‑141 (MQ=255)

                                                                                                                                tGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTaaaa             >  3:143038/1‑141 (MQ=255)

                                                                                                                                tGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTaaaa             >  4:232816/1‑141 (MQ=255)

                                                                                                                                 gCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTaaaaa            >  1:294653/1‑141 (MQ=255)

                                                                                                                                    agaTGTTTATTACGTATTCCATGCAGCAGCATTAAAAAAAGTGCCGTCATGTGAATTCTTTCCAGAAGAGGCAGTGAAGACAAATATTATTGGTACAAAAAATGTCTTACAAATTGCTATTCAAAAAAAAAAAAAAAAAGt         <  2:236699/141‑1 (MQ=255)

                                                                                                                                    agaTGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGAGCTATTCATCAAAATGTTAAAAAAGt         >  2:860601/1‑141 (MQ=255)

                                                                                                                                     gaTGTTTATTACGTATTCCATGCAGCAGCTGTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTAAAAAAGTc        >  2:975225/1‑141 (MQ=255)

                                                                                                                                        gTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTAAAAAAGTCata     >  3:88970/1‑141 (MQ=255)

                                                                                                                                          ttattaCGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTAAAAAAGTCATATg   <  2:570127/141‑1 (MQ=255)

                                                                                                                                           tattaCGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTGCCAGTAGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAAAAAAAAAAAAGTCATATGt  <  2:932356/141‑1 (MQ=255)

TATTAAAGAAATTCGTATTTTTTCACGCGATGAGAAAAAACAAGATGACATTCGAAAAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTGAAGACAAATATTATTGGTACAGAAAATGTCTTACAAAGTGCTATTCATCAAAATGTTAAAAAAGTCATATGT  >  USA300TCH1516_ALE/178109‑178388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`