BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,654,172	T→A	5.0%	G145G (GGT→GGA)	USA300HOU_RS13650 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,654,172	0	T	A	5.0%	450.1 / 12.5	139	G145G (GGT→GGA)	USA300HOU_RS13650	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (68/64); new base A (2/5); total (70/69)
Fisher's exact test for biased strand distribution p-value = 2.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.14e-01

Only 100 of 142 total aligned reads displayed.

TATTATGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGA  >  USA300TCH1516_ALE/2654034‑2654309

tattatGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTCAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGgtgt                                                                                                                                         >  1:103248/1‑141 (MQ=255)

     tGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTAccc                                                                                                                                    <  2:350235/141‑1 (MQ=255)

      gACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTAcccc                                                                                                                                   >  4:154396/1‑141 (MQ=255)

       ccTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGGTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCa                                                                                                                                  <  2:1011840/140‑1 (MQ=255)

        cTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAt                                                                                                                                 >  1:298549/1‑141 (MQ=255)

           cAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAtttt                                                                                                                              <  3:136600/141‑1 (MQ=255)

           cAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAtttt                                                                                                                              >  2:116568/1‑141 (MQ=255)

           cAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAtttt                                                                                                                              >  1:780533/1‑141 (MQ=255)

            aGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAttttt                                                                                                                             >  2:736614/1‑141 (MQ=255)

             ggtggtGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTa                                                                                                                            >  1:886586/1‑141 (MQ=255)

             ggaggtgCGTCAACCCTTCCTCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTa                                                                                                                            <  2:73686/138‑1 (MQ=255)

                  tgCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGc                                                                                                                       >  2:698159/1‑141 (MQ=255)

                    cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                     <  1:356072/141‑1 (MQ=255)

                    cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                     >  1:724541/1‑141 (MQ=255)

                    cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                     >  1:622767/1‑141 (MQ=255)

                     gTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGAt                                                                                                                    >  1:738066/1‑141 (MQ=255)

                         aCCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTc                                                                                                                <  4:283004/141‑1 (MQ=255)

                         aCCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTc                                                                                                                >  2:282334/1‑141 (MQ=255)

                            aTTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCAtt                                                                                                             <  2:533735/141‑1 (MQ=255)

                            aTTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCAtt                                                                                                             >  1:84525/1‑141 (MQ=255)

                            aTTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCAtt                                                                                                             <  3:238943/141‑1 (MQ=255)

                             ttCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTa                                                                                                            >  3:4162/1‑141 (MQ=255)

                              tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCCACCCTGTTTCTACAATTCGATCGTTTCATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                           <  1:488338/141‑1 (MQ=255)

                              tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                           >  3:148367/1‑141 (MQ=255)

                              tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                           <  1:947317/141‑1 (MQ=255)

                              tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                           <  1:991300/141‑1 (MQ=255)

                               cgtcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATg                                                                                                          >  3:398535/3‑141 (MQ=255)

                                 tcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGAt                                                                                                        <  3:173297/141‑1 (MQ=255)

                                   aaaGTTTCAACTTACCGTATTGGCTGAGCGCTCTCATATTAGTCGTCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTg                                                                                                      <  2:126801/141‑1 (MQ=255)

                                     aGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCg                                                                                                    >  1:625725/1‑141 (MQ=255)

                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                      >  2:822589/1‑135 (MQ=255)

                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                      <  1:822589/135‑1 (MQ=255)

                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                      <  1:822697/135‑1 (MQ=255)

                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCCTTATGATTGCGGTct                                                                                                >  4:81240/1‑141 (MQ=255)

                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTct                                                                                                <  2:684092/141‑1 (MQ=255)

                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTct                                                                                                >  1:178647/1‑141 (MQ=255)

                                         tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGCct                                                                                                >  3:258562/1‑141 (MQ=255)

                                          cAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTcta                                                                                               >  2:768659/1‑141 (MQ=255)

                                          cAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGActa                                                                                               >  1:539437/1‑141 (MQ=255)

                                            aCTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTctact                                                                                             <  3:178707/141‑1 (MQ=255)

                                              ttACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTAt                                                                                           >  1:444396/1‑141 (MQ=255)

                                                     aTTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCacaa                                                                                    <  1:990426/141‑1 (MQ=255)

                                                      ttGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTCATTGCGATTGTCATTATGATTGCGGGCTACTATTTCacaac                                                                                   >  2:106226/1‑141 (MQ=255)

                                                      ttGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCacaac                                                                                   >  2:325646/1‑141 (MQ=255)

                                                       tGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCacaaca                                                                                  >  3:190151/1‑141 (MQ=255)

                                                               cgcACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTCCTATTTCACAACAAGTTATCt                                                                          >  3:1154/1‑141 (MQ=255)

                                                               cgcACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCt                                                                          >  3:79472/1‑141 (MQ=255)

                                                                    tCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGAtt                                                                     >  3:77484/1‑141 (MQ=255)

                                                                       tatTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTa                                                                  <  2:139240/141‑1 (MQ=255)

                                                                        atTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTAc                                                                 <  2:964018/141‑1 (MQ=255)

                                                                          tAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTg                                                               <  2:1080244/141‑1 (MQ=255)

                                                                             tCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCg                                                            <  1:422127/141‑1 (MQ=255)

                                                                                ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTGCGGTCTACTATTTCACAACGAGTCATCTTGATTTTACTGCCGCta                                                         <  1:410927/141‑1 (MQ=255)

                                                                                ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTGCGGTCTACTATTTCACAACGAGTCATCTTGATTTTACTGCCGCta                                                         <  1:1046421/141‑1 (MQ=255)

                                                                                ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCtc                                                         <  1:1030610/141‑2 (MQ=255)

                                                                                    tATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCtaataa                                                     <  2:467099/141‑1 (MQ=255)

                                                                                     aTTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATTATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTaataat                                                    <  3:214764/141‑1 (MQ=255)

                                                                                     aTTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTaataat                                                    >  2:300783/1‑141 (MQ=255)

                                                                                     aTTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTaataat                                                    >  2:806459/1‑141 (MQ=255)

                                                                                      ttttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatg                                                   >  4:300818/1‑141 (MQ=255)

                                                                                      ttttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatg                                                   >  3:591/1‑141 (MQ=255)

                                                                                        ttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatgat                                                 <  1:766676/141‑1 (MQ=255)

                                                                                         tagaaaCAGTGTTGCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatgatg                                                >  1:228137/5‑141 (MQ=255)

                                                                                          aGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGc                                               <  1:385709/141‑1 (MQ=255)

                                                                                           gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                              >  2:592010/1‑141 (MQ=255)

                                                                                           gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                              >  1:707687/1‑141 (MQ=255)

                                                                                           gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                              <  1:724916/141‑1 (MQ=255)

                                                                                           gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                              <  3:402081/141‑1 (MQ=255)

                                                                                              acacTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAg                                           <  2:411678/141‑1 (MQ=255)

                                                                                              acacTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGACTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAg                                           <  2:539437/141‑1 (MQ=255)

                                                                                                 cTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGAtt                                        >  1:662063/1‑141 (MQ=255)

                                                                                                   gTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCa                                      <  4:364490/141‑1 (MQ=255)

                                                                                                    tttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCAt                                     >  4:72772/1‑141 (MQ=255)

                                                                                                    tttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCAt                                     <  1:944274/141‑1 (MQ=255)

                                                                                                    tttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCAt                                     <  1:311521/141‑1 (MQ=255)

                                                                                                      tCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATaa                                   <  1:518813/141‑1 (MQ=255)

                                                                                                        tAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGc                                 <  4:127933/141‑1 (MQ=255)

                                                                                                         aaaaTTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTCCCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCa                                <  1:942558/141‑1 (MQ=255)

                                                                                                          aaaTTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAg                               >  3:345261/1‑141 (MQ=255)

                                                                                                              tCGCTCGTTTAATTTCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAAt                           <  2:340212/141‑1 (MQ=255)

                                                                                                               cGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATc                          >  2:451789/1‑141 (MQ=255)

                                                                                                               cGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATc                          <  1:415019/141‑1 (MQ=255)

                                                                                                                   cGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTa                      >  2:105480/1‑141 (MQ=255)

                                                                                                                    gTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTAt                     <  1:53838/141‑1 (MQ=255)

                                                                                                                    gTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTAt                     <  2:1014765/141‑1 (MQ=255)

                                                                                                                        aaTTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCAcc                 <  1:619872/141‑1 (MQ=255)

                                                                                                                          ttGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTg               >  3:372882/1‑141 (MQ=255)

                                                                                                                          ttGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTg               <  2:625967/141‑1 (MQ=255)

                                                                                                                           tGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATGATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTgg              <  2:933884/141‑1 (MQ=255)

                                                                                                                           tGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTgg              >  2:993280/1‑141 (MQ=255)

                                                                                                                             cTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAt            <  1:785981/141‑1 (MQ=255)

                                                                                                                              tgtgCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAtg           >  2:162629/1‑141 (MQ=255)

                                                                                                                               gtgCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACTTGGAtgg          >  4:316344/1‑141 (MQ=255)

                                                                                                                                 gCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAtggtg        <  1:880663/141‑1 (MQ=255)

                                                                                                                                   ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt      <  2:586348/141‑1 (MQ=255)

                                                                                                                                   ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt      <  1:282334/141‑1 (MQ=255)

                                                                                                                                   ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt      <  1:251530/141‑1 (MQ=255)

                                                                                                                                    tGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGtt     <  4:381261/141‑1 (MQ=255)

                                                                                                                                    tGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGtt     <  2:994056/141‑1 (MQ=255)

                                                                                                                                       cGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGa  >  1:194854/1‑141 (MQ=255)

TATTATGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGA  >  USA300TCH1516_ALE/2654034‑2654309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`