Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,763,245 T→A 5.1% V265V (GTA→GTT cysJ ← Sulfite reductase [NADPH] flavoprotein alpha‑component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,763,2450TA 5.1% 428.4 / 8.3 133V265V (GTA→GTTcysJSulfite reductase [NADPH] flavoprotein alpha‑component
Reads supporting (aligned to +/- strand):  ref base T (47/79);  new base A (2/5);  total (49/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 134 total aligned reads displayed.

AACCAATTCAGGGTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAATGATGTCTGCCATCCACTTTTCTGC  >  USA300TCH1516_ALE/2763107‑2763385
                                                                                                                                           |                                                                                                                                            
aaCCAATTCAGGGTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATAc                                                                                                                                            <  2:618821/141‑1 (MQ=255)
aaCCAATTCAGGGTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATAc                                                                                                                                            <  3:392085/141‑1 (MQ=255)
aaCCAATTCAGGGTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATAc                                                                                                                                            <  2:757146/141‑1 (MQ=255)
 aCCAATTCAGGGTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACt                                                                                                                                           <  1:1072210/141‑1 (MQ=255)
  ccAATTCAGGGTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACtt                                                                                                                                          <  1:691808/141‑1 (MQ=255)
  ccAATTCAGGGTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACtt                                                                                                                                          <  4:44643/141‑1 (MQ=255)
   cAATTCAGGGTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTc                                                                                                                                         >  1:866824/1‑141 (MQ=255)
     aTTCAGGGTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTg                                                                                                                                       >  4:33641/1‑141 (MQ=255)
         aGGGTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTg                                                                                                                                   >  1:434154/1‑141 (MQ=255)
         aGGGTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTg                                                                                                                                   >  2:43629/1‑141 (MQ=255)
          gggTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTgg                                                                                                                                  >  2:642987/1‑141 (MQ=255)
          gggTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTgg                                                                                                                                  >  3:21039/1‑141 (MQ=255)
           ggTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGt                                                                                                                                 >  2:873556/1‑141 (MQ=255)
            gTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTTACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTa                                                                                                                                >  4:144101/1‑141 (MQ=255)
            gTCGTTGTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTa                                                                                                                                <  1:352058/141‑1 (MQ=255)
              cGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATg                                                                                                                              <  2:73980/141‑1 (MQ=255)
               gTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATgg                                                                                                                             >  1:763309/1‑141 (MQ=255)
                ttttGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGga                                                                                                                            <  1:298692/141‑1 (MQ=255)
                 tttGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGgat                                                                                                                           <  1:770117/141‑1 (MQ=255)
                 ttcgcagTACTGATACTATACAATCACCTGGTTCATATTATTCACTAAAAAAATAAAAAAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGCTATTCTCTTATACTTCTGCTTGGTATGgat                                                                                                                           <  2:273249/135‑1 (MQ=255)
                   tGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGgattt                                                                                                                         <  3:337713/141‑1 (MQ=255)
                      ggTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGgatttgat                                                                                                                      <  1:588499/141‑1 (MQ=255)
                        tAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAAAACTTCTGCTTGGTATGgatttaattt                                                                                                                    <  1:205473/141‑1 (MQ=255)
                         aaTGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAACTTCTATATGTCGTGTTTCTTTCTTTGAATCGGTACCATTTAAATTGATGTTCGCTAATACTTCTGCTTGGTCTGGCTTTGATTTa                                                                                                                   >  1:1050788/1‑141 (MQ=255)
                           tGCTACTATACAATCTCCTGGTTCATATTATTCACTAAAATCATCAAATAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGa                                                                                                                 <  4:45799/141‑1 (MQ=255)
                           tGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGa                                                                                                                 >  4:285034/1‑141 (MQ=255)
                           tGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGa                                                                                                                 <  4:369248/141‑1 (MQ=255)
                               actaTACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGGTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAAAACTTCTGCTTGGTATGGATTTAATTTAGAatat                                                                                                             <  1:915502/141‑1 (MQ=255)
                                ctaTACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATAtt                                                                                                            <  2:901017/141‑1 (MQ=255)
                                 tataCAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATAttt                                                                                                           <  2:457967/141‑1 (MQ=255)
                                   taCAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATAtttct                                                                                                         <  1:372945/141‑1 (MQ=255)
                                   taCAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATAtttct                                                                                                         <  4:37405/141‑1 (MQ=255)
                                           ccTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTg                                                                                                 >  4:8227/1‑141 (MQ=255)
                                               gTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTGTTTGTGTTGAACGTTACTGTTTATGTTGAAATTCTCGAATACTGCTTCTTGGTATGGATTGTATTTAGAATATTTCTTTTCTTTGGCAg                                                                                             >  1:918976/1‑141 (MQ=255)
                                               gTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAg                                                                                             <  3:116122/141‑1 (MQ=255)
                                                ttCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGa                                                                                            <  3:274402/141‑1 (MQ=255)
                                                    tatGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTa                                                                                        <  2:203482/141‑1 (MQ=255)
                                                     atGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTCAGAATATTTCTTTTCTTTGGCAGACTTaa                                                                                       <  1:735458/141‑1 (MQ=255)
                                                       gATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAAtt                                                                                     <  1:95636/141‑1 (MQ=255)
                                                          tCACTAAAATCATCAAGTAAAAATTCTATTTGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGAt                                                                                  <  1:780816/141‑1 (MQ=255)
                                                            aCTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTGAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTc                                                                                <  3:314997/141‑1 (MQ=255)
                                                             cTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCa                                                                               >  2:563255/1‑141 (MQ=255)
                                                               aaaaTCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAACTGATATTCGCTCATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACt                                                                             <  4:81892/141‑1 (MQ=255)
                                                                 aaTCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTa                                                                           >  2:819452/1‑141 (MQ=255)
                                                                  atcatcAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTAt                                                                          >  2:763937/1‑141 (MQ=255)
                                                                  atcatcAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTAt                                                                          >  1:409556/1‑141 (MQ=255)
                                                                     atcaAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCAc                                                                       <  2:664910/141‑1 (MQ=255)
                                                                      tcaAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCAcc                                                                      <  1:173511/141‑1 (MQ=255)
                                                                       caAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCg                                                                     <  1:161935/141‑1 (MQ=255)
                                                                          gTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGAtt                                                                  <  4:171292/141‑1 (MQ=255)
                                                                          gTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGAtt                                                                  >  1:150902/1‑141 (MQ=255)
                                                                             aaaaTTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAAAACTTCTGCTTGGTATGGATTTAATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCAc                                                               <  2:980983/141‑1 (MQ=255)
                                                                              aaaTTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTACTACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACt                                                              <  2:874539/141‑1 (MQ=255)
                                                                              aaaTTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACt                                                              <  1:743280/141‑1 (MQ=255)
                                                                              aaaTTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACt                                                              <  2:193324/141‑1 (MQ=255)
                                                                               aaTTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAAAACTTCTGCTTGGTATGGATTTAATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACtt                                                             >  3:67820/1‑141 (MQ=255)
                                                                                        tGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCt                                                    >  1:92185/1‑141 (MQ=255)
                                                                                        tGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCt                                                    <  1:131142/141‑1 (MQ=255)
                                                                                        tGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCt                                                    <  2:114930/141‑1 (MQ=255)
                                                                                        tGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAAAACTTCTGCTTGGTATGGATTTAATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCt                                                    <  4:262653/141‑1 (MQ=255)
                                                                                           cGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCt                                                 >  3:397975/1‑141 (MQ=255)
                                                                                            gtgtTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTTATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTg                                                <  2:409865/141‑1 (MQ=255)
                                                                                            gtgtTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTg                                                <  2:478208/141‑1 (MQ=255)
                                                                                            gtgtTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTg                                                <  2:189281/141‑1 (MQ=255)
                                                                                               tttCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGAtg                                             <  2:218627/141‑1 (MQ=255)
                                                                                                ttCTTTATTTGAAGCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGAtgt                                            <  2:709725/141‑1 (MQ=255)
                                                                                                   tttATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGt                                         <  3:402848/141‑1 (MQ=255)
                                                                                                   tttATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGCATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACTTTATGATGTGGt                                         >  2:686031/1‑141 (MQ=255)
                                                                                                   tttATTTGAATCGGTACCATTTAAATTGATATTCGCTAAAACTTCTGCTTGGTATGGATTTAATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGAtgtagt                                         <  1:912494/141‑4 (MQ=255)
                                                                                                     tATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTAt                                       >  1:529199/1‑141 (MQ=255)
                                                                                                         ttGAATCGGTACCATTTAAATTGATATCCGCTAATACTTCTGCGTGGTATGCTTTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGGTTCACTTATCACCGAGTCACTTTGACTACCTTCTGATCTGGTAACaa                                    >  4:360625/1‑141 (MQ=255)
                                                                                                          tGAATCGGTACCGTTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGGTTTGATTTAGAATATTTCTTTTCTTTAGCAGACTTAATTGTTTCACTTATCACCGATTCACTTTGAATACCTTCTGATGCGGTATCaat                                   <  2:706110/141‑1 (MQ=255)
                                                                                                          tGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCaat                                   <  1:388673/141‑1 (MQ=255)
                                                                                                          tGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCaat                                   <  1:392068/141‑1 (MQ=255)
                                                                                                          tGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCaat                                   <  3:389051/141‑1 (MQ=255)
                                                                                                          tGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGCATTTGATTTAGAATATTCCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCaat                                   <  4:391819/141‑1 (MQ=255)
                                                                                                            aaTCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCaataa                                 >  4:379917/1‑141 (MQ=255)
                                                                                                               cGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAatat                              <  2:291767/141‑1 (MQ=255)
                                                                                                               cGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAatat                              <  4:411708/141‑1 (MQ=255)
                                                                                                                ggTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATAtt                             >  1:100579/1‑141 (MQ=255)
                                                                                                                 gTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTa                            >  1:484534/1‑141 (MQ=255)
                                                                                                                 gTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTa                            <  1:166123/141‑1 (MQ=255)
                                                                                                                  tACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTaa                           <  1:334802/141‑1 (MQ=255)
                                                                                                                  tACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTaa                           >  1:221757/1‑141 (MQ=255)
                                                                                                                  tACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTaa                           >  2:825294/1‑141 (MQ=255)
                                                                                                                  tACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTaa                           <  1:334979/141‑1 (MQ=255)
                                                                                                                   aCCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAat                          <  4:216303/141‑1 (MQ=255)
                                                                                                                    ccATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAatg                         >  2:800186/1‑141 (MQ=255)
                                                                                                                     cATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAatga                        <  1:893213/141‑1 (MQ=255)
                                                                                                                        ttAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAATGATGt                     <  2:570902/141‑1 (MQ=255)
                                                                                                                          aaaTTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAATGATGTCt                   <  3:315167/141‑1 (MQ=255)
                                                                                                                            aTTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAATGATGTCTGc                 <  3:258284/141‑1 (MQ=255)
                                                                                                                               gATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAATGATGTCTGCCAt              >  1:144467/1‑141 (MQ=255)
                                                                                                                               gATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAATGATGTCTGCCAt              >  1:27879/1‑141 (MQ=255)
                                                                                                                                atatTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAATGATGTCTGCCATc             <  2:345360/141‑1 (MQ=255)
                                                                                                                                  atTCGCTACTACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAATGATGTCTGCCATCCa           <  2:527299/141‑1 (MQ=255)
                                                                                                                                     cGCTAAAACTTCTGCTTGGTATGGATTTAATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTAGTATCAATCATATTAATGATGTCTGCCATCCACtt        >  3:427465/1‑141 (MQ=255)
                                                                                                                                       cTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTGACTTATCACCGAGTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAATGATGTCTGCCATCCACtttt      >  3:42094/1‑141 (MQ=255)
                                                                                                                                          aTACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAATGATGTCTGCCATCCACTTTTCTg   <  2:996638/141‑1 (MQ=255)
                                                                                                                                           tACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAATGATGTCTGCCATCCACTTTTCTGc  <  2:121109/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AACCAATTCAGGGTCGTTTTGCGGTAATGCTACTATACAATCTCCTGGTTCATATGATTCACTAAAATCATCAAGTAAAAATTCTATATGTCGTGTTTCTTTATTTGAATCGGTACCATTTAAATTGATATTCGCTAATACTTCTGCTTGGTATGGATTTGATTTAGAATATTTCTTTTCTTTGGCAGACTTAATTGATTCACTTATCACCGATTCACTTTGAATACCTTCTGATGTGGTATCAATAATATTAATGATGTCTGCCATCCACTTTTCTGC  >  USA300TCH1516_ALE/2763107‑2763385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: