Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000731 12,012 A→G 5.4% intergenic (+175/+48) USA300HOU_pUSA300HOUMR0014 → / ← USA300HOU_pUSA300HOUMR0015 recombinase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073112,0120AG 5.4% 733.0 / 21.9 244intergenic (+175/+48)USA300HOU_pUSA300HOUMR0014/USA300HOU_pUSA300HOUMR0015recombinase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (102/128);  new base G (2/11);  total (105/139)
Fisher's exact test for biased strand distribution p-value = 4.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-01

Only 100 of 250 total aligned reads displayed.

AAATAATTTTATATATCATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGACTGGCATAA  >  CP000731/11872‑12151
                                                                                                                                            |                                                                                                                                           
 aataatTTTATATATCATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACaa                                                                                                                                            >  2:890887/1‑141 (MQ=255)
  ataatTTTATATATCATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCTTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACaaa                                                                                                                                           >  1:503959/1‑141 (MQ=255)
    aatTTTATATATCATTAACATACAAAGACTTTGATTCAATATCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACAAAAAAAAAAAGCGTACAAAtt                                                                                                                                         <  4:355286/141‑1 (MQ=255)
      ttttATATATCATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCg                                                                                                                                       <  1:203421/141‑1 (MQ=255)
      ttttATATATCATTAACATACAAAGACTTTGATNCAATGTCACTCGATGTAAAAGAGACGTTTTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACANATTCg                                                                                                                                       >  1:390047/1‑141 (MQ=255)
         tatataTCATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACGAATTTGTAc                                                                                                                                    <  3:128496/141‑1 (MQ=255)
         tatataTCATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAAGATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTAc                                                                                                                                    <  1:578429/141‑1 (MQ=255)
          atatatCATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACg                                                                                                                                   <  2:149300/141‑1 (MQ=255)
          atatatCATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACg                                                                                                                                   <  1:1061524/141‑1 (MQ=255)
          atatatCATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACg                                                                                                                                   <  2:937249/141‑1 (MQ=255)
           tatatCATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGc                                                                                                                                  <  1:149470/141‑1 (MQ=255)
            atatCATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATACTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCt                                                                                                                                 <  1:1083840/141‑1 (MQ=255)
               tCATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCtttt                                                                                                                              >  2:532323/1‑141 (MQ=255)
                cATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCttttt                                                                                                                             >  2:507359/1‑141 (MQ=255)
                    aaCATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATACTCACACAAGCTGAATGCGTACAAATTCGTACGCTTTTTtaat                                                                                                                         <  2:48713/141‑1 (MQ=255)
                      aaTACAAACACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCTTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACGAATTTGTACGCttttttcttgt                                                                                                                       <  2:541269/140‑6 (MQ=39)
                       aTACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTtaatata                                                                                                                      >  2:34064/1‑141 (MQ=255)
                            aaGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATAcc                                                                                                                 <  3:154052/141‑1 (MQ=255)
                            aaGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATAcc                                                                                                                 <  2:211312/141‑1 (MQ=255)
                            aaGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATAcc                                                                                                                 <  2:663491/141‑1 (MQ=255)
                               aCTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGt                                                                                                              <  1:246083/141‑1 (MQ=255)
                               aCTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGt                                                                                                              <  1:319264/141‑1 (MQ=255)
                                 tttGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGttt                                                                                                            >  2:420237/1‑141 (MQ=255)
                                  ttGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTc                                                                                                           >  2:99577/1‑141 (MQ=255)
                                  ttGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTc                                                                                                           <  2:834991/141‑1 (MQ=255)
                                    gATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCAc                                                                                                         >  1:654900/1‑141 (MQ=255)
                                          aTGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGa                                                                                                   <  1:185362/141‑1 (MQ=255)
                                               aCTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGtt                                                                                              <  3:310273/141‑1 (MQ=255)
                                                  cGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGGAAAAAGATTACAAATTCGGACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTa                                                                                           >  1:376327/1‑141 (MQ=255)
                                                    aTGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAg                                                                                         <  4:443469/141‑1 (MQ=255)
                                                     tGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGt                                                                                        <  4:21853/141‑1 (MQ=255)
                                                        aaaaGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATc                                                                                     <  3:14060/141‑1 (MQ=255)
                                                         aaaGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCt                                                                                    <  2:950040/141‑1 (MQ=255)
                                                          aaGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCtt                                                                                   >  1:325330/1‑141 (MQ=255)
                                                            gagaCGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTtca                                                                                 >  4:234908/1‑141 (MQ=255)
                                                              gaCGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAACGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTtcatc                                                                               >  2:129062/1‑141 (MQ=255)
                                                                cGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTcatcat                                                                             >  3:21149/1‑141 (MQ=255)
                                                                cGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTcatcat                                                                             <  2:581513/141‑1 (MQ=255)
                                                                cGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTcatcat                                                                             <  2:362454/141‑1 (MQ=255)
                                                                 gTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCAtt                                                                            <  2:44620/141‑1 (MQ=255)
                                                                    cTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAAc                                                                         >  1:674741/1‑141 (MQ=255)
                                                                     tGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACt                                                                        <  2:411090/141‑1 (MQ=255)
                                                                      gACAGCGTCTAAAATCTAATAATATTTTCCTGTTATGCGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACTCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCAGTAAcgg                                                                       <  4:376387/141‑3 (MQ=255)
                                                                         aGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGaaa                                                                    <  1:256571/141‑1 (MQ=255)
                                                                           cGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACt                                                                  <  3:364689/141‑1 (MQ=255)
                                                                               tAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCGTTAACTGAAACTACAg                                                              <  4:393272/141‑1 (MQ=255)
                                                                                aaaaTCTCATAATATTTTCCTGTTATGAGATTTTACTAATAACACAAGAAAAAATCGTACAAATTCGTACGCTTTTTTAATACAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGt                                                             >  2:245557/1‑141 (MQ=255)
                                                                                aaaaTCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGt                                                             >  3:44718/1‑141 (MQ=255)
                                                                                aaaaTCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGt                                                             <  1:296249/141‑1 (MQ=255)
                                                                                aaaaTCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGt                                                             >  4:326400/1‑141 (MQ=255)
                                                                                 aaaTCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGta                                                            <  2:327737/141‑1 (MQ=255)
                                                                                 aaaTCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGta                                                            <  1:517951/141‑1 (MQ=255)
                                                                                 aaaTCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGta                                                            <  2:402200/141‑1 (MQ=255)
                                                                                  aaTCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGtaa                                                           >  1:269472/1‑141 (MQ=255)
                                                                                  aaTCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGtaa                                                           >  1:307431/1‑141 (MQ=255)
                                                                                  aaTCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGtaa                                                           >  1:951136/1‑141 (MQ=255)
                                                                                   aTCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGtaat                                                          <  1:880788/141‑1 (MQ=255)
                                                                                    tctcATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGtaata                                                         >  4:389284/1‑141 (MQ=255)
                                                                                        ataataTTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATAc                                                     >  2:471148/1‑141 (MQ=255)
                                                                                        ataataTTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGAACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATAc                                                     >  1:930899/1‑141 (MQ=255)
                                                                                          aataTTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTc                                                   >  3:369218/1‑141 (MQ=255)
                                                                                           atatTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCg                                                  >  2:728524/1‑141 (MQ=255)
                                                                                             atTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTc                                                <  1:835391/141‑1 (MQ=255)
                                                                                              ttttCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTca                                               <  1:895790/141‑1 (MQ=255)
                                                                                              ttttCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTca                                               <  1:365984/141‑1 (MQ=255)
                                                                                              ttttCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTca                                               <  3:417282/141‑1 (MQ=255)
                                                                                              ttttCCGGTTATGAGATTTTAATAATAACACAAGAAACAAGCGTAAAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCAATATTCTTCGTAGCTACAGTAATAATACTCGTca                                               <  4:225669/141‑1 (MQ=255)
                                                                                                ttCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCTTcaac                                             >  2:1014151/1‑141 (MQ=255)
                                                                                                ttCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTcaac                                             <  2:795382/141‑1 (MQ=255)
                                                                                                ttCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTcaac                                             >  2:62237/1‑141 (MQ=255)
                                                                                                  ccTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTcaacaa                                           >  3:322527/1‑141 (MQ=255)
                                                                                                  ccTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTcaacaa                                           >  1:1010216/1‑141 (MQ=255)
                                                                                                   cTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAat                                          >  1:1030059/1‑141 (MQ=255)
                                                                                                   cTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTAGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAat                                          >  3:164802/1‑141 (MQ=255)
                                                                                                    tGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAata                                         <  3:222736/141‑1 (MQ=255)
                                                                                                       tATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTa                                      >  1:628233/1‑141 (MQ=255)
                                                                                                          gagaTTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGta                                   <  4:21149/141‑1 (MQ=255)
                                                                                                          gagaTTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGta                                   <  2:1056144/141‑1 (MQ=255)
                                                                                                          gagaTTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGta                                   >  3:393275/1‑141 (MQ=255)
                                                                                                             aTTTTAATAATAACACACGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATAATCGCCACCACTATTAGTATAg                                >  2:973204/1‑141 (MQ=255)
                                                                                                             aTTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAg                                <  1:411826/141‑1 (MQ=255)
                                                                                                              ttttAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGt                               <  3:107032/141‑1 (MQ=255)
                                                                                                               tttAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGtt                              <  4:112583/141‑1 (MQ=255)
                                                                                                                  aataataaCACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTaaa                           <  1:687619/141‑1 (MQ=255)
                                                                                                                    taataaCACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAAt                         <  1:562956/141‑1 (MQ=255)
                                                                                                                     aataaCACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATa                        <  2:77842/141‑1 (MQ=255)
                                                                                                                       taaCACAAGAAAAAACCGTACAAACTCGTACGCTTTTCTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTAGCTTCATCATTAACTGAAACTACAGTATTAATACTCGTCAACAATATCAGTATAGATAAAATAGa                      >  2:310768/1‑141 (MQ=255)
                                                                                                                                aaaaaaGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAg             <  2:572906/141‑1 (MQ=255)
                                                                                                                                  aaaaGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATAATTAAATGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGa           <  1:432409/141‑1 (MQ=255)
                                                                                                                                   aaaGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGAc          >  2:85216/1‑141 (MQ=255)
                                                                                                                                     aGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGACTg        <  1:456843/141‑1 (MQ=255)
                                                                                                                                      gCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGACTgg       <  2:76813/141‑1 (MQ=255)
                                                                                                                                      gCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGACTgg       >  1:61840/1‑141 (MQ=255)
                                                                                                                                       cGTACGAATTTGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGACTGGc      >  1:760989/1‑141 (MQ=255)
                                                                                                                                       cGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGACTGGc      <  4:140774/141‑1 (MQ=255)
                                                                                                                                       cGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGACAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGACTGGc      <  4:295644/141‑1 (MQ=255)
                                                                                                                                        gTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGACTGGCa     >  3:444876/1‑141 (MQ=255)
                                                                                                                                           cAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGACTGGCATaa  <  1:795578/141‑1 (MQ=255)
                                                                                                                                           cAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGACTGGCATaa  <  1:984101/141‑1 (MQ=255)
                                                                                                                                           cAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGACTGGCATaa  <  4:68660/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
AAATAATTTTATATATCATTAACATACAAAGACTTTGATTCAATGTCACTCGATGTAAAAGAGACGTTCTGACAGCGTCTAAAATCTCATAATATTTTCCTGTTATGAGATTTTAATAATAACACAAGAAAAAAGCGTACAAATTCGTACGCTTTTTTAATATAATACCTGTTTCACTAATGAACGTTTTAAGTATCTTCATCATTAACTGAAACTACAGTAATAATACTCGTCAACAATATTAGTATAGTTAAAATAGAAAGACTTAGGACTGGCATAA  >  CP000731/11872‑12151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: