Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 211,512 T→A 5.2% intergenic (+70/+258) sfp → / ← yagU 4'‑phosphopantetheinyl transferase sfp/Inner membrane protein YagU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE211,5120TA 5.2% 397.5 / 9.5 126intergenic (+70/+258)sfp/yagU4'‑phosphopantetheinyl transferase sfp/Inner membrane protein YagU
Reads supporting (aligned to +/- strand):  ref base T (61/57);  new base A (3/4);  total (65/61)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

Only 100 of 136 total aligned reads displayed.

GGTATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCATTTGAG  >  USA300TCH1516_ALE/211373‑211651
                                                                                                                                           |                                                                                                                                           
ggTATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGttt                                                                                                                                            >  1:491675/1‑141 (MQ=255)
 gTATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGtttt                                                                                                                                           <  3:421653/141‑1 (MQ=255)
  tATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGttttt                                                                                                                                          >  3:384657/1‑141 (MQ=255)
  tATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGttttt                                                                                                                                          >  2:12389/1‑141 (MQ=255)
  tATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGttttt                                                                                                                                          <  1:995148/141‑1 (MQ=255)
   aTGTTTCTTAGGACAGGCACCCTGGGTTTATAAAAAAGTGTCCGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGtttttt                                                                                                                                         >  1:253561/1‑141 (MQ=255)
     gTTTCTTAGGACATGCACACTGGGGTCATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTa                                                                                                                                       <  4:211775/141‑1 (MQ=255)
        tCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTAttt                                                                                                                                    >  2:290173/1‑140 (MQ=255)
         cTTAAGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGGCGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTgg                                                                                                                                   <  3:329954/141‑1 (MQ=255)
          ttAGGACAGGCACCCTGTGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATGGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTCCTGTTAGATGTTTTTTTATTGGa                                                                                                                                  <  4:15436/141‑1 (MQ=255)
          ttAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGa                                                                                                                                  <  3:247768/141‑1 (MQ=255)
          ttAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGa                                                                                                                                  <  1:167336/141‑1 (MQ=255)
          ttAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGa                                                                                                                                  <  1:504852/141‑1 (MQ=255)
          ttAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGa                                                                                                                                  <  4:334078/141‑1 (MQ=255)
          ttAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGACTAAATGTTTACTGTTAGATGTTTTTTTATTGGa                                                                                                                                  >  1:1005696/1‑141 (MQ=255)
           tAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGaa                                                                                                                                 <  1:906213/141‑1 (MQ=255)
            aGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTTTTGGaaa                                                                                                                                >  2:977820/1‑141 (MQ=255)
             ggACAGGCAACCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGaaaa                                                                                                                               <  4:247017/141‑1 (MQ=255)
                gaGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTATTTTTATTGGAAAAATg                                                                                                                            >  4:349660/2‑141 (MQ=255)
                cAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATg                                                                                                                            <  1:677946/141‑1 (MQ=255)
                  ggCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGaa                                                                                                                          >  1:445470/1‑141 (MQ=255)
                   gCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGaaa                                                                                                                         >  1:992495/1‑141 (MQ=255)
                         tGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGaa                                                                                                                   >  1:580792/1‑141 (MQ=255)
                         tGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGaa                                                                                                                   <  3:227685/141‑1 (MQ=255)
                           gggTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATa                                                                                                                 <  1:290132/141‑1 (MQ=255)
                              ttATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATacac                                                                                                              >  4:258778/1‑141 (MQ=255)
                               tataAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACg                                                                                                             >  3:170649/1‑141 (MQ=255)
                                ataAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGa                                                                                                            <  3:70226/141‑1 (MQ=255)
                                 taAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGAt                                                                                                           >  2:889478/1‑141 (MQ=255)
                                  aaaaaaGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATg                                                                                                          >  4:297488/1‑141 (MQ=255)
                                     aaaGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGAGCTCGTAGTTTAAACGATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGca                                                                                                       <  1:19467/141‑1 (MQ=255)
                                      aaGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGcat                                                                                                      <  3:5027/141‑1 (MQ=255)
                                      aaGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTACTGGAAAAATGAAAGGTGAATACACGATGGcat                                                                                                      <  4:36086/141‑1 (MQ=255)
                                       aGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGcatc                                                                                                     >  1:694643/1‑141 (MQ=255)
                                          gtCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCAtt                                                                                                  >  3:378647/1‑141 (MQ=255)
                                           tCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTc                                                                                                 >  1:160454/1‑141 (MQ=255)
                                            cTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCa                                                                                                >  2:665581/1‑141 (MQ=255)
                                               tATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCaaaa                                                                                             <  4:51388/141‑1 (MQ=255)
                                                 tttCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATc                                                                                           <  3:302401/141‑1 (MQ=255)
                                                   tCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAATCTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCAt                                                                                         >  2:635252/1‑141 (MQ=255)
                                                   tCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCAt                                                                                         <  2:34476/141‑1 (MQ=255)
                                                      gTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTTTTGGATATATGAAAGGTGAATACACGAAGGCATCATTCAAAATTATgtc                                                                                      >  2:29732/1‑139 (MQ=255)
                                                      gTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAAGGAAAGGTGAATACACGATGGCATCATTCAAAATCATGAc                                                                                      >  2:4810/1‑141 (MQ=255)
                                                          ttGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACgaga                                                                                  >  1:114237/1‑141 (MQ=255)
                                                          ttGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACgaga                                                                                  >  2:239549/1‑141 (MQ=255)
                                                           tGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTATTTTTATTTGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGAt                                                                                 <  3:349660/141‑1 (MQ=255)
                                                            gAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGAtt                                                                                >  1:968945/1‑141 (MQ=255)
                                                                 gtTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAaagt                                                                           <  3:88200/141‑1 (MQ=255)
                                                                  ttAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAaagta                                                                          <  1:223769/141‑1 (MQ=255)
                                                                  ttAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAaagta                                                                          <  1:309267/141‑1 (MQ=255)
                                                                  ttAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTATAAAAATAGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAaagta                                                                          <  2:965626/141‑1 (MQ=255)
                                                                       atGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGa                                                                     >  4:304075/1‑141 (MQ=255)
                                                                           cGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAg                                                                 >  3:229236/1‑141 (MQ=255)
                                                                           cGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAg                                                                 >  1:816030/1‑141 (MQ=255)
                                                                           cGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAg                                                                 >  1:425209/1‑141 (MQ=255)
                                                                           cGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGATTACTGGTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAg                                                                 >  1:170285/1‑141 (MQ=255)
                                                                            gTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTTTTTTTTTTATGAAAGGTGAATACACGATGGCATCATTCAAAATAATGAGGAGCTTAAAGTAAGTGATAAgg                                                                >  4:250753/1‑141 (MQ=255)
                                                                             tCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATCAAAGTAAGTGATAAGGt                                                               >  3:24160/1‑141 (MQ=255)
                                                                              cttaCAGTATCATGGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATTTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTa                                                              <  1:61687/138‑1 (MQ=255)
                                                                              cTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTa                                                              >  2:788859/1‑141 (MQ=255)
                                                                                gACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTaaa                                                            >  1:1080643/1‑141 (MQ=255)
                                                                                 aCAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTaaaa                                                           <  2:107404/141‑1 (MQ=255)
                                                                                  cAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTaaaaa                                                          <  1:363270/141‑1 (MQ=255)
                                                                                          ttGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGa                                                  >  4:180234/1‑141 (MQ=255)
                                                                                          ttGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGa                                                  <  1:931249/141‑1 (MQ=255)
                                                                                            gcgcAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGc                                                >  4:167485/1‑141 (MQ=255)
                                                                                                 gTGATTTTAAACTATACTGCCTCAATGTTTTCTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCAGGACGAGATTAAAGTAAGTGTTAAGGTAAAAATATACTGAGCAatat                                           <  3:379475/141‑1 (MQ=255)
                                                                                                 gTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAatat                                           <  1:600461/141‑1 (MQ=255)
                                                                                                  tGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATAtt                                          <  2:9040/141‑1 (MQ=255)
                                                                                                       ttAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTGTTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAAt                                     >  1:894670/1‑141 (MQ=255)
                                                                                                       ttAAACTATACTGCATAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGAATCATTCAAAAACATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATAATGgaaa                                     >  2:526498/1‑140 (MQ=255)
                                                                                                        tAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAAtt                                    <  3:418046/141‑1 (MQ=255)
                                                                                                          aaCTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTAcc                                 <  4:161029/141‑1 (MQ=255)
                                                                                                                tttttcTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGaaaa                            <  2:40122/136‑1 (MQ=255)
                                                                                                                aCTGGCTAAATATTTACTGTTAGATGTTTTTATATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGaaaa                            <  2:178701/141‑1 (MQ=255)
                                                                                                                aCTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGaaaa                            <  2:142109/141‑1 (MQ=255)
                                                                                                                aCTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGaaaa                            <  2:375055/141‑1 (MQ=255)
                                                                                                                aCTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGAGTAAAGTAAGGGATAAGGTAAAAATATACTGAGCAATAATGAAATTACCGaaaa                            >  3:180073/1‑141 (MQ=255)
                                                                                                                 cTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAaat                           <  2:939609/141‑1 (MQ=255)
                                                                                                                  tGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAaata                          <  3:219858/141‑1 (MQ=255)
                                                                                                                   gCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAaataa                         <  2:1010476/141‑1 (MQ=255)
                                                                                                                    ccTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAaataat                        >  1:295492/1‑141 (MQ=255)
                                                                                                                       aaaTGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGt                     >  3:283085/1‑141 (MQ=255)
                                                                                                                        aaTGTTTTCTGTAAGAAGATTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACAGAGAAATAAACAAAAAAACAAATATAATAGtt                    <  2:766875/141‑1 (MQ=255)
                                                                                                                          tGTTTACTGTTAGATGTTTTTTTTTTTTTTTTTTTAAAGGAGAATACACGCTGGCTTTATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTAAGCAATATTGAAATTACCGATTTTATTAGtttt                  >  2:703580/1‑140 (MQ=255)
                                                                                                                          tGTTTACTGTTAGATGTATTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAATGTAAGTGATAAGGTGAAAATATACTGAGCAATATTGAAATTGCCAAAAataatatcttg                  <  2:696612/141‑6 (MQ=255)
                                                                                                                          tGTTCACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGGAGACACGATGGCATCATTCAAAAGCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGATCAATATTGAAATTACCGAAAATAATAGTTTg                  >  4:438554/1‑141 (MQ=255)
                                                                                                                           gTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGGGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGt                 >  3:154542/1‑141 (MQ=255)
                                                                                                                            tttACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGtt                >  1:939728/1‑141 (MQ=255)
                                                                                                                             ttACTGTTAGATGTTTTTTTTTTTTTTTTATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATGAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTa               >  2:309878/1‑141 (MQ=255)
                                                                                                                              tACTGTTAGATGTATTTTTATTGGAAAAATGAAAGGTGAATACATGATGGCATCATTCAAAATCATGACGAGATTAATGTAAGTGATAAGGTGAAAATATACTGAGCAATATTGAAATTGCCAAAAATAATATCTTGTTac              <  1:415987/141‑1 (MQ=255)
                                                                                                                               aCTGTTAGATGTATTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAATGTAAGTGATAAGGTGAAAATATACTGAGCAATATTGAAATTGCCAAAAATAATATCTTGTTacg             >  1:352992/1‑141 (MQ=255)
                                                                                                                                cTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTacga            <  1:711128/141‑1 (MQ=255)
                                                                                                                                 tGTTAGATGTATTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAATGTAAGTGATAAGGTGAAAATATACTGAGCAATATTGAAATTGCCAAAAATAATATCTTGTTacgac           >  1:485488/1‑141 (MQ=255)
                                                                                                                                  gTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATCTACTGCGCAATAGTGAAATTACCGAAAATAATAGTTTGTTacgacg          >  2:555386/1‑141 (MQ=255)
                                                                                                                                  gTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTacgacg          <  1:75742/141‑1 (MQ=255)
                                                                                                                                  gTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGAAAAAAAAAAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTacgacg          <  2:445470/141‑1 (MQ=255)
                                                                                                                                       aTGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCAttt     >  1:682973/1‑141 (MQ=255)
                                                                                                                                       aTGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCAttt     >  2:875502/1‑141 (MQ=255)
                                                                                                                                        tGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCATTTg    <  4:261808/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GGTATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCATTTGAG  >  USA300TCH1516_ALE/211373‑211651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: