Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 336,153 A→G 5.8% G97G (GGA→GGG essC → ESAT‑6 secretion machinery protein EssC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE336,1530AG 5.8% 399.8 / 11.7 137G97G (GGA→GGGessCESAT‑6 secretion machinery protein EssC
Reads supporting (aligned to +/- strand):  ref base A (78/51);  new base G (2/6);  total (80/57)
Fisher's exact test for biased strand distribution p-value = 6.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-01

Only 100 of 139 total aligned reads displayed.

ACTTGGCAAGCGAATCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGTATATTAACTATGAACTACA  >  USA300TCH1516_ALE/336013‑336293
                                                                                                                                            |                                                                                                                                            
  ttGGCAAGCGAATCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGAcc                                                                                                                                            >  3:114495/1‑141 (MQ=255)
  ttGGCAAGCGAATCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGAcc                                                                                                                                            <  1:16661/141‑1 (MQ=255)
    ggCAAGCGAATCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCaa                                                                                                                                          <  2:1080309/141‑1 (MQ=255)
     gCAAGCGAATCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCaaa                                                                                                                                         >  1:921736/1‑141 (MQ=255)
     gCAAGCGAATCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAAATTGACCaaa                                                                                                                                         >  2:74026/1‑141 (MQ=255)
      cAAGCGAATCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGGCCAAAt                                                                                                                                        <  3:345879/141‑1 (MQ=255)
      cAAGCGAATCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAAt                                                                                                                                        >  1:678070/1‑141 (MQ=255)
      cAAGCGAATCATACTTCTATTAATAAGGTGCCGGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCGGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAAt                                                                                                                                        >  4:379569/1‑141 (MQ=255)
       aaGCGAATCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTTGACCAAATg                                                                                                                                       >  2:301488/1‑141 (MQ=255)
       aaGCGAATCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATg                                                                                                                                       >  1:756882/1‑141 (MQ=255)
           gAATCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTa                                                                                                                                   >  3:59203/1‑141 (MQ=255)
           gAATCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTa                                                                                                                                   >  1:354159/1‑141 (MQ=255)
            aaTCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTat                                                                                                                                  <  1:626615/141‑1 (MQ=255)
             aTCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTatg                                                                                                                                 <  4:182070/141‑1 (MQ=255)
             aTCACACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTatg                                                                                                                                 <  3:411810/141‑1 (MQ=255)
                 tACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTAtgatga                                                                                                                             <  2:790210/141‑1 (MQ=255)
                 tACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTAtgatga                                                                                                                             >  2:727063/1‑141 (MQ=255)
                  aCTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATgatgat                                                                                                                            <  2:439222/141‑1 (MQ=255)
                   cTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGata                                                                                                                           <  3:216783/141‑1 (MQ=255)
                    ttCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGatat                                                                                                                          >  2:401571/1‑141 (MQ=255)
                      cTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATgg                                                                                                                        <  4:320371/141‑1 (MQ=255)
                       tATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGt                                                                                                                       >  3:50914/1‑141 (MQ=255)
                         ttAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTa                                                                                                                     <  1:970884/141‑1 (MQ=255)
                         ttAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTa                                                                                                                     >  2:477690/1‑141 (MQ=255)
                          taataaGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTAt                                                                                                                    <  4:408881/141‑1 (MQ=255)
                          taataaGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTAt                                                                                                                    <  4:60904/141‑1 (MQ=255)
                          taataaGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTAt                                                                                                                    >  4:259176/1‑141 (MQ=255)
                            ataaGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACCGAATCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTc                                                                                                                  <  3:96976/141‑1 (MQ=255)
                            ataaGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGGCCAAATGCGTATGATGATATGGTTATTc                                                                                                                  <  2:88600/141‑1 (MQ=255)
                            ataaGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTc                                                                                                                  <  1:644860/141‑1 (MQ=255)
                            ataaGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTc                                                                                                                  <  1:880548/141‑1 (MQ=255)
                             taaGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATGCAGAAGCTAAGTATGTGTTATTTGCGTATCCTTCAATGCTAGATACGATGACGATTGGACCAAATGCGTATGATGATATTGTTATTCa                                                                                                                 >  1:102756/1‑141 (MQ=255)
                             taaGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCa                                                                                                                 >  2:680333/1‑141 (MQ=255)
                             taaGGTGCTTGTTAGAAAAGGGGACCTTGCTGACATTACATTACAGCTGTATAGAGGAGCTGATTGTGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACGATTGGAGCAAATGGGAATGAGGCTATGGTGGTTCa                                                                                                                 >  2:918184/1‑141 (MQ=255)
                                 gTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGc                                                                                                             <  3:257996/141‑1 (MQ=255)
                                 gTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGc                                                                                                             >  3:328223/1‑141 (MQ=255)
                                 gTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGc                                                                                                             >  2:433771/1‑141 (MQ=255)
                                  tGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCt                                                                                                            <  3:273317/141‑1 (MQ=255)
                                  tGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCt                                                                                                            >  1:266955/1‑141 (MQ=255)
                                   gCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCtt                                                                                                           >  2:465789/1‑141 (MQ=255)
                                     ttGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTtga                                                                                                         >  1:771130/1‑141 (MQ=255)
                                       gTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGGCCAAATGCGTATGATGATATGGTTATTCAAAGCTtgatg                                                                                                       <  2:764959/141‑1 (MQ=255)
                                         tAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGaa                                                                                                     <  2:693826/141‑1 (MQ=255)
                                          aGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAAt                                                                                                    <  1:620088/141‑1 (MQ=255)
                                             aaaGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGcc                                                                                                 <  2:192252/141‑1 (MQ=255)
                                             aaaGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGcc                                                                                                 >  1:713110/1‑141 (MQ=255)
                                             aaaGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGcc                                                                                                 >  1:51114/1‑141 (MQ=255)
                                              aaGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCca                                                                                                >  1:140175/1‑141 (MQ=255)
                                                ggagACCTTGCTGTCGTTACATGACAGCATGATACAGAAGCTGGTTAAGCATCTTTTGCGGGTCCGTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATTATTTGTGTATTCAAAGCTTTTTAAATGCcatc                                                                                              >  4:78093/4‑141 (MQ=255)
                                                ggTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCGTTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGGGTATGATGATAGGGTTATTCAACGCTTGATGAATGCcatc                                                                                              >  1:550866/1‑141 (MQ=255)
                                                ggTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCcatc                                                                                              <  1:296879/141‑1 (MQ=255)
                                                ggTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCcatc                                                                                              >  4:397809/1‑141 (MQ=255)
                                                  tGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCcatcat                                                                                            <  2:887841/141‑1 (MQ=255)
                                                   gACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCatt                                                                                           >  2:950513/1‑141 (MQ=255)
                                                         gatgaCATTACATTACTGCTTTATCCAGAGGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGTTGACAATTGGATCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATAATTATTaaa                                                                                     <  4:29912/141‑1 (MQ=255)
                                                             acattacattACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGGCCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGAtt                                                                                 <  2:274183/141‑1 (MQ=255)
                                                               attacattacAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGAtttt                                                                               >  1:777962/1‑141 (MQ=255)
                                                                  acattacaGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTtcaa                                                                            >  2:830496/1‑141 (MQ=255)
                                                                  acattacaGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTtcaa                                                                            >  1:693248/1‑141 (MQ=255)
                                                                    attacaGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTtcaatc                                                                          <  2:600242/141‑1 (MQ=255)
                                                                         aGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATAc                                                                     >  3:186861/1‑141 (MQ=255)
                                                                         aGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATAc                                                                     >  3:40230/1‑141 (MQ=255)
                                                                         aGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATAc                                                                     <  2:619452/141‑1 (MQ=255)
                                                                               ataCAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAAt                                                               >  2:521898/1‑141 (MQ=255)
                                                                                  cAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAACTGCGTATGATGATATGGCTATTCAAAGCTTGATCCATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATcac                                                            >  4:207021/1‑141 (MQ=255)
                                                                                  cAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATcac                                                            <  1:355263/141‑1 (MQ=255)
                                                                                       gCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAAtac                                                       >  2:9904/1‑141 (MQ=255)
                                                                                         tGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAAtacgt                                                     >  1:528329/1‑141 (MQ=255)
                                                                                            ttATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGCTGATATGGTTAGTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAAtacgtacg                                                  >  2:602127/1‑141 (MQ=255)
                                                                                            ttATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGATTGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAAtacgtacg                                                  <  3:349268/141‑1 (MQ=255)
                                                                                            ttATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAAtgcgtacg                                                  <  1:269460/141‑1 (MQ=255)
                                                                                            ttATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAAtacgtacg                                                  >  2:479922/1‑141 (MQ=255)
                                                                                            ttATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAAtacgtacg                                                  <  4:113339/141‑1 (MQ=255)
                                                                                            ttATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAAtacgtacg                                                  >  2:793289/1‑141 (MQ=255)
                                                                                                   tcatTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATACCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATtgtg                                           <  1:477690/141‑1 (MQ=255)
                                                                                                         gCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGAt                                     <  3:38255/141‑1 (MQ=255)
                                                                                                          cGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATa                                    >  1:947511/1‑141 (MQ=255)
                                                                                                          cGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATa                                    >  2:1072460/1‑141 (MQ=255)
                                                                                                          cGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATa                                    >  2:986666/1‑141 (MQ=255)
                                                                                                           gTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAACAATCACAATACGTACGCATTGTCCACGATaa                                   >  1:75501/1‑141 (MQ=255)
                                                                                                              tCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATaaaaa                                <  1:465789/141‑1 (MQ=255)
                                                                                                                cTTCAATTCAAGCTACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATa                              <  2:384103/141‑1 (MQ=255)
                                                                                                                cTTCAATTCAAGATACGATGACAATTGGGCCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATa                              <  2:761896/141‑1 (MQ=255)
                                                                                                                 ttcaattcaaGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATAc                             <  2:1049194/141‑1 (MQ=255)
                                                                                                                        caaGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGAtgtg                      >  2:17980/1‑141 (MQ=255)
                                                                                                                         aaGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATgtgt                     >  2:341487/1‑141 (MQ=255)
                                                                                                                          aGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGta                    >  4:220884/1‑141 (MQ=255)
                                                                                                                          aGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGta                    >  1:79098/1‑141 (MQ=255)
                                                                                                                           gATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGtat                   >  3:209016/1‑141 (MQ=255)
                                                                                                                              aCGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGAGTATAtt                >  2:739778/1‑141 (MQ=255)
                                                                                                                               cGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGTATATTa               >  2:36175/1‑141 (MQ=255)
                                                                                                                               cGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGTATATTa               >  3:205116/1‑141 (MQ=255)
                                                                                                                                 aTGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGTATATTAAc             <  2:1044538/141‑1 (MQ=255)
                                                                                                                                    aCAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGTATATTAACTAt          >  2:516016/1‑141 (MQ=255)
                                                                                                                                     cAATTGGGCCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGTATATTAACTATg         <  3:431061/141‑1 (MQ=255)
                                                                                                                                     cAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGTATATTAACTATg         <  3:383605/141‑1 (MQ=255)
                                                                                                                                       aTTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATAAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGTATATTAACTATGaa       <  3:178141/141‑1 (MQ=255)
                                                                                                                                         tGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGTATATTAACTATGAACt     >  2:1070236/1‑141 (MQ=255)
                                                                                                                                           gACCAAATGCGTATGANGATATGGTTATTNAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGTATATTAACTATGAACTAc   >  1:910228/1‑141 (MQ=255)
                                                                                                                                            aCCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGTATATTAACTATGAACTACa  >  2:77725/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ACTTGGCAAGCGAATCATACTTCTATTAATAAGGTGCTTGTTAGAAAAGGTGACCTTGATGACATTACATTACAGCTTTATACAGAAGCTGATTATGCATCATTTGCGTATCCTTCAATTCAAGATACGATGACAATTGGACCAAATGCGTATGATGATATGGTTATTCAAAGCTTGATGAATGCCATCATTATTAAAGATTTTCAATCAATACAAGAATCACAATACGTACGCATTGTGCACGATAAAAATACAGATGTGTATATTAACTATGAACTACA  >  USA300TCH1516_ALE/336013‑336293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: