Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 491,299 G→T 6.1% intergenic (+57/‑275) metQ_1 → / → sle1_1 putative D‑methionine‑binding lipoprotein MetQ/N‑acetylmuramoyl‑L‑alanine amidase sle1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE491,2990GT 6.1% 464.7 / 10.5 138intergenic (+57/‑275)metQ_1/sle1_1putative D‑methionine‑binding lipoprotein MetQ/N‑acetylmuramoyl‑L‑alanine amidase sle1
Reads supporting (aligned to +/- strand):  ref base G (67/62);  new base T (7/2);  total (74/64)
Fisher's exact test for biased strand distribution p-value = 1.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

GGAAGATGTCAAAGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATAC  >  USA300TCH1516_ALE/491161‑491438
                                                                                                                                          |                                                                                                                                           
ggAAGATGTCAAAGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGga                                                                                                                                           <  2:67435/141‑1 (MQ=255)
        tCAAAGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATaaaa                                                                                                                                   <  1:286100/141‑1 (MQ=255)
         cAAAGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAat                                                                                                                                  <  1:339586/141‑1 (MQ=255)
          aaaGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAatt                                                                                                                                 >  1:226538/1‑141 (MQ=255)
                 gagagaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCa                                                                                                                          >  2:482984/1‑141 (MQ=255)
                   gagaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAAt                                                                                                                        >  2:283128/1‑141 (MQ=255)
                   gagaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAAt                                                                                                                        >  1:639352/1‑141 (MQ=255)
                   gagaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAAt                                                                                                                        >  1:460150/1‑141 (MQ=255)
                     gaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATag                                                                                                                      <  2:404141/141‑1 (MQ=255)
                     gaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATag                                                                                                                      <  2:1052677/141‑1 (MQ=255)
                       aaCCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATagag                                                                                                                    <  3:336429/141‑1 (MQ=255)
                          cTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGaaa                                                                                                                 >  4:221314/1‑141 (MQ=255)
                           tGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGTCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAg                                                                                                                >  1:102537/1‑141 (MQ=255)
                            gTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAgg                                                                                                               >  1:549361/1‑141 (MQ=255)
                            gTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAgg                                                                                                               >  2:358131/1‑141 (MQ=255)
                            gTTAATTTATCAAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAgg                                                                                                               >  4:131689/1‑141 (MQ=255)
                               aaTTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGAt                                                                                                            <  4:123906/141‑1 (MQ=255)
                                aTTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGAtt                                                                                                           <  2:964616/141‑1 (MQ=255)
                                     tCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAAt                                                                                                      <  2:940976/141‑1 (MQ=255)
                                      cTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAAtt                                                                                                     >  2:409234/1‑140 (MQ=255)
                                      cTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATc                                                                                                     <  1:61154/141‑1 (MQ=255)
                                             tGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTtat                                                                                              <  1:593423/141‑1 (MQ=255)
                                             tGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTtat                                                                                              <  2:905027/141‑1 (MQ=255)
                                             tGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTtat                                                                                              <  2:905119/141‑1 (MQ=255)
                                              gagaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTtata                                                                                             >  1:518105/1‑141 (MQ=255)
                                               agaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTTATAg                                                                                            >  2:718791/1‑141 (MQ=255)
                                                gaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGt                                                                                           >  2:280327/1‑141 (MQ=255)
                                                gaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGt                                                                                           >  2:763428/1‑141 (MQ=255)
                                                  ttAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTCGGGCGTTATTGTTGGAGACTAAAATTCTTTCCAATAGAGAAGGGGATTGTAATCATTTTATAGTGa                                                                                         <  1:973687/141‑1 (MQ=255)
                                                  ttAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGa                                                                                         >  4:166125/1‑141 (MQ=255)
                                                  ttAAAGAAATAGAAACGTCATTAGCAAAATAAATAATATTGCATACAACAAGCAAAGTTCATGCTTATGTTAGTCGGAAGATAATGTTGGAGCCTACAATTATTATCAATCCAGAAAGGGACTTAAATCATTTTATAGTGa                                                                                         <  1:973900/141‑1 (MQ=255)
                                                      ggCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTACATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTGTTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAata                                                                                     >  3:16494/1‑141 (MQ=255)
                                                        cAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAAtatt                                                                                   >  4:108430/1‑141 (MQ=255)
                                                         aaTAGAAACGTCATGAGGAAAATAAATTATATGGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAAtatta                                                                                  <  2:31613/141‑1 (MQ=255)
                                                          aTAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAAtattat                                                                                 <  2:886822/141‑1 (MQ=255)
                                                           tAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATg                                                                                >  1:111236/1‑141 (MQ=255)
                                                           tAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATg                                                                                <  1:283128/141‑1 (MQ=255)
                                                             gAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTTATAGTGAAATATTATGaa                                                                              >  1:1020180/1‑141 (MQ=255)
                                                                aCGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAAtt                                                                           >  1:188079/1‑141 (MQ=255)
                                                                 cGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTg                                                                          <  4:7789/141‑1 (MQ=255)
                                                                 cGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTg                                                                          <  1:325299/141‑1 (MQ=255)
                                                                  gTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGt                                                                         <  3:224920/141‑1 (MQ=255)
                                                                   tCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGta                                                                        <  1:2711/141‑1 (MQ=255)
                                                                    cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                       >  2:993274/1‑141 (MQ=255)
                                                                    cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                       <  3:32101/141‑1 (MQ=255)
                                                                    cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                       >  4:18305/1‑141 (MQ=255)
                                                                    cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                       >  1:124287/1‑141 (MQ=255)
                                                                    cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTACGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                       >  2:84968/1‑141 (MQ=255)
                                                                     aTTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaat                                                                      >  1:532624/1‑141 (MQ=255)
                                                                        aGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTaataat                                                                   <  2:169965/141‑1 (MQ=255)
                                                                        aGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAAGTGTaataat                                                                   >  2:376722/1‑141 (MQ=255)
                                                                        aGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATCTTTGTAGGGCGTTATTGTTGGATAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTaataat                                                                   <  1:375912/141‑1 (MQ=255)
                                                                           aaaaTAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTa                                                                <  1:423623/141‑1 (MQ=255)
                                                                             aataaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGa                                                              >  2:409367/1‑141 (MQ=255)
                                                                               taaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGata                                                            >  2:1041924/1‑141 (MQ=255)
                                                                                aaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGatat                                                           <  3:752/141‑1 (MQ=255)
                                                                                  atTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTg                                                         <  1:48734/141‑1 (MQ=255)
                                                                                  atTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTg                                                         >  2:733269/1‑141 (MQ=255)
                                                                                  atTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTg                                                         <  3:131545/141‑1 (MQ=255)
                                                                                   ttATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGt                                                        <  4:431752/141‑1 (MQ=255)
                                                                                     atatTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTaa                                                      <  3:131698/141‑1 (MQ=255)
                                                                                      tatTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTaaa                                                     <  1:251540/141‑1 (MQ=255)
                                                                                       atTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTaaaa                                                    >  4:136575/1‑141 (MQ=255)
                                                                                        ttGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAAt                                                   >  1:952538/1‑141 (MQ=255)
                                                                                         tGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATc                                                  >  3:454555/1‑141 (MQ=255)
                                                                                         tGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATc                                                  <  1:269087/141‑1 (MQ=255)
                                                                                            gTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCtaa                                               >  1:862810/1‑141 (MQ=255)
                                                                                            gTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCtaa                                               >  1:66578/1‑141 (MQ=255)
                                                                                            gTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCtaa                                               >  3:358635/1‑141 (MQ=255)
                                                                                                 aCAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGt                                          <  1:667591/141‑1 (MQ=255)
                                                                                                      cAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaa                                     <  2:737375/141‑1 (MQ=255)
                                                                                                        aaGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                   >  1:878433/1‑141 (MQ=255)
                                                                                                        aaGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                   >  4:268835/1‑141 (MQ=255)
                                                                                                         aGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaataa                                  <  2:469343/141‑1 (MQ=255)
                                                                                                          gTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTaataat                                 >  1:717143/1‑141 (MQ=255)
                                                                                                            tCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAAttt                               <  2:71694/141‑1 (MQ=255)
                                                                                                             cATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAAtttt                              >  1:906408/1‑141 (MQ=255)
                                                                                                              aTGCTTATGTTTGTAGGGCGTTATTGTTTGACAAAATATTTATTTCCAATAGAGAAAGGGTATGTAATCATGTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTa                             <  2:300226/141‑1 (MQ=255)
                                                                                                              aTGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTa                             >  1:764522/1‑141 (MQ=255)
                                                                                                                gCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATTGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAg                           >  2:618124/1‑141 (MQ=255)
                                                                                                                     tGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTa                      >  2:1069518/1‑141 (MQ=255)
                                                                                                                     tGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTa                      >  4:403266/1‑141 (MQ=255)
                                                                                                                     tGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTa                      <  2:331611/141‑1 (MQ=255)
                                                                                                                       tttGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAAt                    >  1:853895/1‑141 (MQ=255)
                                                                                                                       tttGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAAt                    <  1:1033491/141‑1 (MQ=255)
                                                                                                                        ttGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAAtt                   <  3:208044/141‑1 (MQ=255)
                                                                                                                        ttGTAGGGCGTTATTGTTGAAGAATAAAATTATTTCCAATAGAGAAAGGGTTTGTAATCATATTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATATTTTTAAGGGGTAAtt                   <  4:53108/141‑1 (MQ=255)
                                                                                                                         tGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATta                  >  2:163126/1‑141 (MQ=255)
                                                                                                                          gTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtat                 <  1:30043/141‑1 (MQ=255)
                                                                                                                          gTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtat                 <  1:445895/141‑1 (MQ=255)
                                                                                                                          gTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtat                 <  4:373457/141‑1 (MQ=255)
                                                                                                                          gTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtat                 <  2:518105/141‑1 (MQ=255)
                                                                                                                           tAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtata                >  2:1070771/1‑141 (MQ=255)
                                                                                                                           tAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtata                >  2:960458/1‑141 (MQ=255)
                                                                                                                           tAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtata                >  3:258238/1‑141 (MQ=255)
                                                                                                                            aGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATaa               >  1:783673/1‑141 (MQ=255)
                                                                                                                                 gTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAAttt          <  3:221314/141‑1 (MQ=255)
                                                                                                                                    aTTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTtgat       <  2:629270/141‑1 (MQ=255)
                                                                                                                                      tgttgGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTtgatga     <  1:995972/141‑1 (MQ=255)
                                                                                                                                         tgGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATAc  <  3:308011/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GGAAGATGTCAAAGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATAC  >  USA300TCH1516_ALE/491161‑491438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: