Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 9,710 T→C 7.3% intergenic (+15/+72) gyrA → / ← nnrD DNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE9,7100TC 7.3% 452.8 / 19.8 164intergenic (+15/+72)gyrA/nnrDDNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (72/78);  new base C (4/8);  total (77/87)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.29e-01

Only 100 of 167 total aligned reads displayed.

AATGCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACAT  >  USA300TCH1516_ALE/9573‑9850
                                                                                                                                                |                                                                                                                                             
  tGCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCta                                                                                                                                          <  2:297164/141‑1 (MQ=255)
    cAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCtata                                                                                                                                        <  2:186298/141‑1 (MQ=255)
    cAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCtaca                                                                                                                                        <  2:338472/141‑3 (MQ=255)
    cAATGCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCtata                                                                                                                                        <  2:552855/141‑1 (MQ=255)
     aaTTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTatat                                                                                                                                       >  1:653850/1‑141 (MQ=255)
     aaTTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTatat                                                                                                                                       >  1:688030/1‑141 (MQ=255)
      aTTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCtacata                                                                                                                                      <  3:370566/141‑5 (MQ=255)
       ttCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGt                                                                                                                                     <  2:476128/141‑1 (MQ=255)
           tACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAggg                                                                                                                                 >  4:351158/1‑141 (MQ=255)
            aCTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAgggg                                                                                                                                >  1:997634/1‑141 (MQ=255)
            aCTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAgggg                                                                                                                                <  2:866194/141‑1 (MQ=255)
            aCTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAggga                                                                                                                                <  1:113654/141‑2 (MQ=255)
               gAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGt                                                                                                                             <  3:413110/141‑1 (MQ=255)
               gAAGTGAGTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGt                                                                                                                             <  2:224369/141‑1 (MQ=255)
                 aGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCt                                                                                                                           <  1:432935/141‑1 (MQ=255)
                 aGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCt                                                                                                                           <  1:433007/141‑1 (MQ=255)
                 aGTGATGGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCt                                                                                                                           <  2:837236/141‑1 (MQ=255)
                  gTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCtt                                                                                                                          <  1:211969/141‑1 (MQ=255)
                       tgattCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTAtttt                                                                                                                    >  3:45603/1‑141 (MQ=255)
                        gattCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTAttttt                                                                                                                   <  2:283433/141‑1 (MQ=255)
                             agaagaAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCTTAttttttatta                                                                                                              <  1:618374/141‑6 (MQ=38)
                                  aaaaTGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCATATATGTCGGGGAGTCTTATTTTTATGCTAGaaa                                                                                                         >  4:349111/1‑141 (MQ=255)
                                    aaTGATGAATGTTGACGTATTCACGGAAGACAACATTTCATCGATCGTTTTTACGATACTCTACAATAATCCGA‑‑‑‑‑‑ACATGAAAAATAATTAACAATAAGACTTCCCTATATGTAGGGGATTCTTATTTTTATGCTCGAAAGt                                                                                                       <  4:167552/141‑1 (MQ=255)
                                    aaTGATGAAGATGGACGTAAGTAATTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGt                                                                                                       <  1:283622/141‑1 (MQ=255)
                                     atgatgAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTa                                                                                                      >  4:27997/1‑141 (MQ=255)
                                      tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                     <  2:990334/141‑1 (MQ=255)
                                      tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                     <  4:30878/141‑1 (MQ=255)
                                       gatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAAt                                                                                                    >  4:451077/1‑141 (MQ=255)
                                              aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTAc                                                                                             <  1:210719/141‑1 (MQ=255)
                                              aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTAc                                                                                             <  2:199616/141‑1 (MQ=255)
                                                ggACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACta                                                                                           >  1:192107/1‑141 (MQ=255)
                                                    gTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATAtt                                                                                       <  4:217761/141‑1 (MQ=255)
                                                     tATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATAAGTAGGGGAGGCTTATTTCTATGCTAGAAAGTAATGCCTTACTATATTc                                                                                      >  2:954296/1‑141 (MQ=255)
                                                        tGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAAt                                                                                   <  2:293606/141‑1 (MQ=255)
                                                            gTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTCTTATGCTAGAAAGTAATGCTTTACTATATTCAATGAtt                                                                               >  2:489259/1‑141 (MQ=255)
                                                             tAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATACTCAATGATTa                                                                              >  2:691956/1‑141 (MQ=255)
                                                               aGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGt                                                                            <  1:564714/141‑1 (MQ=255)
                                                               aGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGt                                                                            >  2:455014/1‑141 (MQ=255)
                                                                gACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTa                                                                           >  1:100784/1‑141 (MQ=255)
                                                                gACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTa                                                                           >  4:335351/1‑141 (MQ=255)
                                                                  cAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGATGAAGAATAATAAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTGTACTATATTCAATGAt                                                                                <  1:451709/141‑1 (MQ=255)
                                                                    aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa                                                                       <  2:940476/141‑1 (MQ=255)
                                                                    aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa                                                                       >  2:192083/1‑141 (MQ=255)
                                                                    aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATATTTATGCTAGAAAGTAATGCTTTACTATATTAAATTATTAGTAAGGa                                                                       >  4:190925/1‑141 (MQ=255)
                                                                       tttCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTa                                                                    <  4:444285/141‑1 (MQ=255)
                                                                       tttCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATCACTa                                                                    >  2:788497/1‑141 (MQ=255)
                                                                         tCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAAc                                                                  >  1:232149/1‑141 (MQ=255)
                                                                         tCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAAc                                                                  >  1:134828/1‑141 (MQ=255)
                                                                          cATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACt                                                                 >  3:53366/1‑141 (MQ=255)
                                                                           aTGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACtt                                                                <  3:199929/141‑1 (MQ=255)
                                                                           aTGGATCATGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACGATATTCAATGATTAGTAATGACTAACtt                                                                >  3:402831/1‑141 (MQ=255)
                                                                            tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt                                                               >  3:122668/1‑141 (MQ=255)
                                                                            tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt                                                               >  1:177473/1‑141 (MQ=255)
                                                                              gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt                                                             <  3:330685/141‑1 (MQ=255)
                                                                              gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt                                                             >  1:1030712/1‑141 (MQ=255)
                                                                              gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt                                                             <  2:321510/141‑1 (MQ=255)
                                                                               aTCGTGTTGAAGAAGATATACAACAATCATG‑‑‑‑‑‑AGATGGAGAATAATAAAAAATAAGACTACCCTATATGAAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTa                                                            >  2:913163/1‑141 (MQ=255)
                                                                                  gtgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAtt                                                         <  1:709536/141‑1 (MQ=255)
                                                                                  gtgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAtt                                                         <  1:671668/141‑1 (MQ=255)
                                                                                   tgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCNATGATTAGTAATNACTAACTTTCTAATTg                                                        <  1:876416/141‑1 (MQ=255)
                                                                                   tgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTg                                                        <  1:111178/141‑1 (MQ=255)
                                                                                    gtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGt                                                       >  2:457960/1‑141 (MQ=255)
                                                                                      tGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGttt                                                     >  1:489802/1‑141 (MQ=255)
                                                                                       gaagaaGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTc                                                    <  3:180200/141‑1 (MQ=255)
                                                                                         agaagaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCAt                                                  >  2:683224/1‑141 (MQ=255)
                                                                                            agaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGc                                               >  4:60491/1‑141 (MQ=255)
                                                                                                  acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                         <  2:892026/141‑1 (MQ=255)
                                                                                                  acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                         <  2:185885/141‑1 (MQ=255)
                                                                                                  acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                         <  1:271121/141‑1 (MQ=255)
                                                                                                  acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAACAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                         <  1:1041746/141‑1 (MQ=255)
                                                                                                   caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                        >  2:393441/1‑141 (MQ=255)
                                                                                                   caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                        >  3:272818/1‑141 (MQ=255)
                                                                                                     acaaTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAt                                      <  1:833678/141‑1 (MQ=255)
                                                                                                         tcatc‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                  <  2:1026836/141‑1 (MQ=255)
                                                                                                         tcatc‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                  >  2:724733/1‑141 (MQ=255)
                                                                                                         tcatc‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                  >  2:666732/1‑141 (MQ=255)
                                                                                                             c‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGa                              >  2:907586/1‑141 (MQ=255)
                                                                                                             c‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGa                              >  2:736408/1‑141 (MQ=255)
                                                                                                                    acaTGAAGAATAATAAATAATAAGACTTCCCCATATGGAGGGCAGTCTTATTTGATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAGTGTTTCATTGCGTAAGGTATTTCATTGATa                            <  3:327034/139‑1 (MQ=255)
                                                                                                                    aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt                             >  2:125287/1‑141 (MQ=255)
                                                                                                                    aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt                             <  1:323896/141‑1 (MQ=255)
                                                                                                                      aTGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATCGATaa                           >  3:68557/1‑141 (MQ=255)
                                                                                                                        gaagaaTAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGt                         >  2:1067252/1‑141 (MQ=255)
                                                                                                                         aagaaTAATAAAAAATAAGACGTCCCTATATGTAGTGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTc                        <  4:415761/141‑1 (MQ=255)
                                                                                                                          agaaTAATAAAAAATAAGACTTCCCTATATGTAGGGGCGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAATTTTCTAATTGTTTCGTTGCGTAAGGTATTTCATTGATGAGTCt                       >  2:548700/1‑141 (MQ=255)
                                                                                                                          agaaTAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCt                       <  2:389844/141‑1 (MQ=255)
                                                                                                                               aataaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCTTTGCGTAAGGTATTTCATTGATAGGTCTTGAtg                  >  1:682025/1‑141 (MQ=255)
                                                                                                                                ataaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg                 >  1:728157/1‑141 (MQ=255)
                                                                                                                                    aaaaaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggtgg              <  1:94283/141‑1 (MQ=255)
                                                                                                                                       aaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcac           <  3:103367/141‑1 (MQ=255)
                                                                                                                                        aTAAGACTCCCCTACATATAGGGAAGTCTTATTTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcac           >  1:528655/1‑141 (MQ=38)
                                                                                                                                         tAAGACTCCCCTACATATAGGGAAGTCTTATTTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcacc          >  3:328910/1‑141 (MQ=38)
                                                                                                                                           aGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCacaaca       >  2:804462/1‑141 (MQ=255)
                                                                                                                                           aGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca       >  1:520281/1‑141 (MQ=255)
                                                                                                                                           aGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca       >  4:14499/1‑141 (MQ=255)
                                                                                                                                           aGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca       >  1:244719/1‑141 (MQ=255)
                                                                                                                                             aCTTCGCTATATATAGGGGAGTCTTATTTTTATGCTATAAAGTAATTCTTTACTATATTTAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacata     <  4:377369/141‑1 (MQ=255)
                                                                                                                                              cTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatac    <  2:635591/141‑1 (MQ=255)
                                                                                                                                                tCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatacat  >  1:698677/1‑141 (MQ=255)
                                                                                                                                                tCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatacat  >  1:815935/1‑141 (MQ=255)
                                                                                                                                                |                                                                                                                                             
AATGCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACAT  >  USA300TCH1516_ALE/9573‑9850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: